FlyBase Gene Report: Dmel\nau

General Information

Symbol

Dmel\nau

Species

D. melanogaster

Name

nautilus

Annotation Symbol

CG10250

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002922

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

nautilus (nau) encodes a protein that belongs to the bHLH family of transcription factors. It is the unique Drosophila ortholog of the mammalian family of bHLH Myogenic Regulatory Factors. It is involved in somatic muscle myogenesis. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

MyoD, Dmyd

Key Links

Genomic Location

Cytogenetic map

95A10-95B1

Sequence location

3R:23,712,814..23,718,357 [-]

Recombination map

3-80

RefSeq locus

NT_033777 REGION:23712814..23718357

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (10 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN000164853

(GO Reference Genome Project, 2011-)

enables protein dimerization activity

inferred from electronic annotation with InterPro:IPR011598, InterPro:IPR036638

(InterPro Project Members, 2004-)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000164853

(GO Reference Genome Project, 2011-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in larval somatic muscle development

inferred from mutant phenotype

(Enriquez et al., 2012)

involved_in muscle organ development

inferred from mutant phenotype

(Wei et al., 2007)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in muscle organ development

inferred from electronic annotation with InterPro:IPR039704

(InterPro Project Members, 2004-)

traceable author statement

(Dworak and Sink, 2002)

involved_in positive regulation of myoblast differentiation

inferred from biological aspect of ancestor with PANTHER:PTN000164853

(GO Reference Genome Project, 2011-)

involved_in regulation of DNA-templated transcription

inferred from electronic annotation with InterPro:IPR039704

(InterPro Project Members, 2004-)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000164853

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Paterson et al., 1991)

located_in nucleus

inferred from direct assay

(Paterson et al., 1991)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000164853

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS -: Basic helix-loop-helix (bHLH) transcription factors are sequence-specific DNA-binding proteins that regulate transcription. They are characterized by a 60 amino acid region comprising a basic DNA binding domain followed by a HLH motif formed from two amphipathic α-helices connected by a loop. bHLH transcription factors form homo- and hetero-dimeric complexes, which bind to a E box consensus sequence. (Adapted from PMID:15186484).

Protein Function (UniProtKB)

May play an important role in the early development of muscle.

(UniProt, P22816)

Summary (Interactive Fly)

transcription factor - myogenic bHLH factor - ortholog of the mammalian family of bHLH Myogenic Regulatory Factors - involved in somatic muscle myogenesis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\nau for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P22816)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

nau-RA

FBtr0084472

NM_057302

1532

332

nau-RB

FBtr0301445

NM_001170231

1520

328

Additional Transcript Data and Comments

Reported size (kB)

1.5 (northern blot)

(Paterson et al., 1991)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

nau-PA

FBpp0083863

36.2

332

8.20

FBpp0290660

35.8

328

8.20

Q4V6U8

NP_001163702

ACZ94997

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

332 (aa); 36 (kD predicted)

(Paterson et al., 1991)

Comments

External Data

Subunit Structure (UniProtKB)

Efficient DNA binding requires dimerization with another bHLH protein.

(UniProt, P22816)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nau using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nau_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.66

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

larval muscle system

(Corbin et al., 1991)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

somatic trunk mesoderm

(Corbin et al., 1991)

embryonic stage 11 -- 12

somatic muscle primordium

(Fisher et al., 2012)

embryonic stage 13

embryonic/larval hypodermal muscle cell | precursor | subset

Comment: dorsal, lateral, and ventral muscle precursors

(Lord et al., 1995)

embryonic stage 13 -- 16

larval somatic muscle cell

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & larval stage

(Paterson et al., 1991)

Additional Descriptive Data

nau is expressed in clusters of cells in the outermost layer of the somatic mesoderm. In neurogenic mutants, nau continues to be expressed in these clusters, but the clusters contain many more cells.

(Corbin et al., 1991)

nau transcripts are detected in embryos and larvae by northern blot analysis. They peak in 9-12hr embryos and then drastically decline.

(Paterson et al., 1991)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

muscle founder cell of muscle cell of dorsal acute muscle 3

(Enriquez et al., 2012)

muscle founder cell of muscle cell of dorsal oblique muscle 5

(Enriquez et al., 2012)

muscle founder cell of dorsal transverse muscle cell

(Enriquez et al., 2012)

abdominal dorsal oblique muscle 3 founder cell

(Enriquez et al., 2012)

muscle founder cell of muscle cell of mesothoracic lateral longitudinal muscle 1

(Enriquez et al., 2012)

muscle founder cell of muscle cell of metathoracic lateral longitudinal muscle 1

(Enriquez et al., 2012)

muscle founder cell of muscle cell of A1-7 lateral longitudinal muscle 1

(Enriquez et al., 2012)

muscle founder cell of muscle cell of mesothoracic dorsal oblique muscle 4

(Enriquez et al., 2012)

muscle founder cell of muscle cell of metathoracic dorsal oblique muscle 4

(Enriquez et al., 2012)

muscle founder cell of muscle cell of A1-7 dorsal oblique muscle 4

(Enriquez et al., 2012)

embryonic stage 11 -- 17

larval muscle system

(Paterson et al., 1991)

organism | segmentally repeated

(Paterson et al., 1991)

embryonic stage 12

muscle founder cell | subset

(Johnson et al., 2013)

embryonic stage 14 -- 17

somatic muscle cell of telson

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral oblique muscle 4 -- muscle cell of abdominal 7 ventral oblique muscle 4

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral acute muscle 2

(Paterson et al., 1991)

ventral oblique muscle cell

(Paterson et al., 1991)

organism | segmentally repeated

(Paterson et al., 1991)

pharyngeal muscle cell

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral oblique muscle 5 -- muscle cell of abdominal 7 ventral oblique muscle 5

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral longitudinal muscle 1

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral acute muscle 1

(Paterson et al., 1991)

anterior spiracle retractor muscle cell

(Paterson et al., 1991)

muscle cell of abdominal 2 ventral oblique muscle 6 -- muscle cell of abdominal 7 ventral oblique muscle 6

(Paterson et al., 1991)

Additional Descriptive Data

nau protein is first detected at embryonic stage 11 by immunolocalization. In each segment, clusters of nuclei on either side of the midline and a smaller lateral cluster are stained. Another small cluster appears later in a dorsal position in the segments from the labium to A7. During stage 11, the number of nau-expressing cells increases 2-3 fold to a total of 30-40 per hemisegment. Differences in the number and pattern of stained cells between the abdominal and more anterior segments are observed. As dorsal closure begins, the level of staining decreases rapidly. Only the nuclei of some muscle precursors and some clusters in the head continue to show high levels of nau protein. After dorsal closure, weak staining is seen in a variety of muscles. Stronger staining persists in the pharyngeal muscles and in muscles of the anterior spiracles and telson.

(Paterson et al., 1991)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Paterson et al., 1991)

located_in nucleus

inferred from direct assay

(Paterson et al., 1991)

Expression Deduced from Reporters

Reporter: P{nau-lacZ.P}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 17

myoblast of larval muscle system

(Paterson et al., 1991)

organism | segmentally repeated

(Paterson et al., 1991)

embryonic stage 14 -- 17

somatic muscle cell of telson

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral acute muscle 2

(Paterson et al., 1991)

muscle cell of abdominal 2 ventral oblique muscle 6 -- muscle cell of abdominal 7 ventral oblique muscle 6

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral oblique muscle 4 -- muscle cell of abdominal 7 ventral oblique muscle 4

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral longitudinal muscle 1

(Paterson et al., 1991)

organism | segmentally repeated

(Paterson et al., 1991)

pharyngeal muscle cell

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral oblique muscle 5 -- muscle cell of abdominal 7 ventral oblique muscle 5

(Paterson et al., 1991)

muscle cell of abdominal 1 ventral acute muscle 1

(Paterson et al., 1991)

anterior spiracle retractor muscle cell

(Paterson et al., 1991)

ventral oblique muscle cell

(Paterson et al., 1991)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\nau in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\nau

View exonic expression by tissue for Dmel\nau

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002922

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42799/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002922

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002922

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002922

Flygut - An atlas of the Drosophila adult midgut

FBgn0002922

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of nau

Transgenic constructs containing regulatory region of nau

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene disrupted in

Df(3R)Exel6195

(Ryder, 2004.4.26)

Df(3R)mbc-15A

(Erickson et al., 1997)

Df(3R)mbc-30

(Erickson et al., 1997)

Df(3R)nau-9

(Keller et al., 1998, Keller et al., 1998)

Df(3R)nau-11a4

(Keller et al., 1998, Keller et al., 1998)

Gene not disrupted in

Df(3R)CA2

(Erickson et al., 1997)

Df(3R)CA15

(Erickson et al., 1997)

Df(3R)mbc-F5.3

(Erickson et al., 1997)

Inferred from location ( 6 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^how-24B

nau^UAS.cKa

lethal | recessive

nau¹⁸⁸

lethal - all die during embryonic stage | recessive

nau^armGFP

lethal - all die during larval stage | recessive

nau^armGFP

partially lethal, with Scer\GAL4^Act5C.PU

nau^RNAi.UAS.cWa

viable

nau¹⁸⁸

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

nau^GD4473

viable, with Scer\GAL4^tin.CΔ4

nau^GD4473

Sterility

Allele

female sterile (with Df(3R)nau-11a4)

sterile | recessive

nau¹⁸⁸

Other Phenotypes

Allele

abnormal locomotor behavior | larval stage

nau^armGFP

flightless, with Scer\GAL4^how-24B

nau^UAS.cKa

visible, with Scer\GAL4^Bx-MS1096

nau^{UAS.ORF.GW.Tag:HA}

Phenotype manifest in

Allele

dorsal vessel wall cell, with Scer\GAL4^how-24B

nau^UAS.cKa

egg

nau^armGFP

egg, with Scer\GAL4^Act.PU

nau^RNAi.UAS.cWa

egg chamber

nau^armGFP

embryonic midgut constriction (with Df(3R)Exel6195)

nau^armGFP

larval somatic muscle cell

larval somatic muscle cell, with Scer\GAL4^Act.PU

nau^RNAi.UAS.cWa

larval somatic muscle cell (with Df(3R)Exel6195)

larval somatic muscle cell | germline clone

nau¹⁸⁸

muscle cell of A1-7 dorsal acute muscle 3

nau¹⁸⁸

muscle cell of A1-7 dorsal acute muscle 3 | embryonic stage

nau¹⁸⁸

muscle cell of A1-7 dorsal oblique muscle 3

nau¹⁸⁸

muscle cell of A1-7 dorsal oblique muscle 4

muscle cell of A1-7 dorsal oblique muscle 4 | germline clone

nau¹⁸⁸

muscle cell of abdominal 1 dorsal acute muscle 3

muscle cell of abdominal 1 dorsal acute muscle 3 | germline clone

nau¹⁸⁸

myoblast, with Scer\GAL4^how-24B

nau^UAS.cKa

presumptive embryonic/larval muscle system, with Scer\GAL4^how-24B, Scer\GAL4^twi.PG

nau^a.UAS

somatic trunk mesoderm, with Scer\GAL4^how-24B

nau^UAS.cKa

wing, with Scer\GAL4^Bx-MS1096

nau^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (49)

Hsap\MYOD1

11 of 14

Yes

Hsap\MYF5

10 of 14

Yes

Hsap\MYF6

6 of 14

Yes

Hsap\MYOG

6 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (43)

Rnor\Myod1

11 of 14

Yes

Rnor\Myf5

10 of 14

Yes

Rnor\Myf6

7 of 14

Yes

Rnor\Myog

6 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (44)

Mmus\Myod1

11 of 14

Yes

Mmus\Myf5

10 of 14

Yes

Mmus\Myf6

7 of 14

Yes

Mmus\Myog

6 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (34)

Xtro\myf5

5 of 13

Yes

Xtro\myf6

4 of 13

Yes

Xtro\myog

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\neurod4

1 of 13

Yes

Xtro\neurod6

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (51)

Drer\myod1

11 of 14

Yes

Drer\myf5

10 of 14

Yes

Drer\myf6

6 of 14

Yes

Drer\myog

6 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-196c10.13

1 of 14

Drer\si:ch211-246m6.4

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (17)

Cele\hlh-1

7 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (22)

Agam\AgaP_AGAP000876

1 of 12

Yes

Agam\AgaP_AGAP000878

1 of 12

Yes

Agam\AgaP_AGAP001448

1 of 12

Yes

Agam\AgaP_AGAP001740

1 of 12

Yes

Agam\AgaP_AGAP001741

1 of 12

Yes

Agam\AgaP_AGAP003739

1 of 12

Yes

Agam\AgaP_AGAP003756

1 of 12

Yes

Agam\AgaP_AGAP003876

1 of 12

Yes

Agam\AgaP_AGAP004299

1 of 12

Yes

Agam\AgaP_AGAP004386

1 of 12

Yes

Agam\AgaP_AGAP004907

1 of 12

Yes

Agam\AgaP_AGAP005930

1 of 12

Yes

Agam\AgaP_AGAP007124

1 of 12

Yes

Agam\AgaP_AGAP007241

1 of 12

Yes

Agam\AgaP_AGAP007696

1 of 12

Yes

Agam\AgaP_AGAP007822

1 of 12

Yes

Agam\AgaP_AGAP007824

1 of 12

Yes

Agam\AgaP_AGAP009227

1 of 12

Yes

Agam\AgaP_AGAP009983

1 of 12

Yes

Agam\AgaP_AGAP011985

1 of 12

Yes

Agam\AgaP_AGAP013335

1 of 12

Yes

Agam\AgaP_AGAP013406

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\UNE10

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:nau. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (22)

1 of 13

amos

1 of 13

ato

1 of 13

cato

1 of 13

crp

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Mnt

1 of 13

net

1 of 13

sage

1 of 13

soul

1 of 13

tap

1 of 13

twi

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MYOD1; myogenic differentiation 1

model of congenital myopathy 17

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MYOD1; myogenic differentiation 1

11 of 14

159970

CONGENITAL MYOPATHY 17; CMYO17

congenital myopathy 17

MYF5; myogenic factor 5

10 of 14

159990

OPHTHALMOPLEGIA, EXTERNAL, WITH RIB AND VERTEBRAL ANOMALIES; EORVA

MYF6; myogenic factor 6

6 of 14

159991

MYOG; myogenin

6 of 14

159980

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

nau - Abd-B

bimolecular fluorescence complementation, fluorescence microscopy

(Baëza et al., 2015)

nau - Antp

bimolecular fluorescence complementation, fluorescence microscopy

(Baëza et al., 2015)

nau - CG12236

two hybrid array

(Shokri et al., 2019)

nau - CG13775

two hybrid array

(Shokri et al., 2019)

nau - CG8314

two hybrid array

(Shokri et al., 2019)

nau - Ets65A

two hybrid array

(Shokri et al., 2019)

nau - Hand

two hybrid

(Tögel et al., 2013)

nau - Scr

bimolecular fluorescence complementation, fluorescence microscopy

(Baëza et al., 2015)

nau - Sox21b

two hybrid array

(Shokri et al., 2019)

nau - Ubx

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018, Baëza et al., 2015)

nau - abd-A

anti tag coimmunoprecipitation, anti tag western blot, bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018, Baëza et al., 2015)

nau - exd

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

nau

enhanceable

(Enriquez et al., 2012)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Efficient DNA binding requires dimerization with another bHLH protein.

(UniProt, P22816 )

Linkouts

BioGRID - A database of protein and genetic interactions.

67737

DroID - A comprehensive database of gene and protein interactions.

FBgn0002922

MIST (protein-protein) - An integrated Molecular Interaction Database

42799

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Myogenesis

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-80

Cytogenetic map

95A10-95B1

Sequence location

3R:23,712,814..23,718,357 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

95A10-95B1

Limits computationally determined from genome sequence between P{PZ}l(3)06906⁰⁶⁹⁰⁶ and P{PZ}l(3)04684⁰⁴⁶⁸⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

95A1-95A10

(determined by in situ hybridisation)

(Ranz et al., 1999)

95A-95A

(determined by in situ hybridisation)

(Abmayr et al., 1992, Michelson et al., 1990)

95A-95B

(determined by in situ hybridisation)

(Paterson et al., 1991)

Experimentally Determined Recombination Data

Location

3-80

(FlyBase, 2016)

3-81.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

66742

w¹¹¹⁸; P{nau-GFP.FPTB}attP40

50607

y¹ v¹; P{TRiP.HMC02974}attP2

31899

y¹ v¹; P{TRiP.JF02187}attP2/TM3, Sb¹

78280

y¹ sc^* v¹ sev²¹; P{TOE.GS02348}attP40

59120

y¹ w^*; Mi{MIC}nau^MI13327 CG10300^MI13327

92177

ru¹ st¹ e¹ nau¹⁸⁸/TM3, P{HZR+6.8Xb}JG1, Sb¹

FlyORF

F001893

M{UAS-nau.ORF.3xHA.GW}ZH-86Fb

NIG-Fly

10250R-1

P{NIG.10250R}1

HMC02974

y¹ v¹; P{TRiP.HMC02974}attP2

VDRC

v51311

w¹¹¹⁸; P{GD4473}v51311

v318146

PBac{fTRG00518.sfGFP-TVPTBF}VK00033

v342131

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01629}attP40/CyO-GFP

More stocks available...

Genomic Clones (24)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (15)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP01012

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0002922

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002922

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Paterson et al., 1991)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Embryos lacking both maternal and zygotic nau function lack a distinct set of muscle fibres. This muscle loss is tolerated, however, such that the loss of both maternal and zygotic nau function does not result in lethality at any stage of development.

(Balagopalan et al., 2001)

nau has a crucial role in embryonic muscle formation.

(Misquitta and Paterson, 1999)

nau is not required for the formation of muscle precursors, but rather plays a role in the differentiation of a subset of muscle precursors into mature muscle fibres in the developing embryo.

(Keller et al., 1998)

Ectopic expression of nau in developing cardioblasts suggests nau induces myogenic differentiation in vivo and may affect the myogenic differentiation program of specific muscle fibres.

(Keller et al., 1997)

wg acts as an inductive signal that influences the expression of nau in the ventral mesoderm.

(Ranganayakulu et al., 1996)

nau RNA expression during development has been studied.

(Abmayr et al., 1992)

Abnormalities in clusters of nau-producing cells appear in the neurogenic mutants, E(spl), N, Dl, bib, neu, mam, and amx, nau and βTub85D, during stage 11, the time of first detectable nau expression. The clusters arise at the correct developmental time and position but contain more cells than wild type clusters. This causes strong ectodermal abnormalities and has effects on mesoderm development.

(Corbin et al., 1991)

Ecol\lacZ reporter gene constructs have demonstrated that the nau gene product is a transiently expressed nuclear protein that serves as a marker for myogenic precursor cells, or a subset of them, in development. These cells may represent muscle founder cells that are crucial in organising and establishing the precise muscle pattern in each segment of the body plan. nau is a distinctive gene and is not a member of a multigene family.

(Paterson et al., 1991)

Isolated from a genomic library at low stringency using a mouse MyoD and rat myogenin probe.

(Michelson et al., 1990)

nau has been cloned and sequenced and the pattern of RNA expression analysed.

(Michelson et al., 1990)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG10250

(Ni et al., 2010.12.1, Perkins et al., 2009)

Dmyd

(Shishido et al., 1993, Paterson et al., 1991)

MYOD

(Morgenstern and Atchley, 1999)

Mdrf

MyoD

(Dubois et al., 2016, Dubois et al., 2007, Furriols et al., 2007, Baylies and Michelson, 2001, Joyner and Martinez-Arias, 2001, Hughes, 1999, Epstein and Bernstein, 1992)

NAU

(Park et al., 2008)

Nau

(Poovathumkadavil and Jagla, 2020, Bischof et al., 2018, Dubois et al., 2016, Baëza et al., 2015, Ettensohn, 2013, Johnson et al., 2013, Shelton et al., 2009)

Name Synonyms

Nautilus

(Lee and Chen, 2019, Dubois et al., 2016, Enriquez et al., 2012, Enriquez et al., 2010, Enriquez et al., 2008)

myogenic determination

(Paterson et al., 1991)

nautilus

(Yi et al., 2025, Baëza et al., 2015, Wang et al., 2015, Johnson et al., 2013, Tögel et al., 2013, Merabet et al., 2011, Huang et al., 2008, Ravulapalli et al., 2008, Dubois et al., 2007, Dubois et al., 2007, Wei et al., 2007, Sandmann et al., 2006, Hossain et al., 2005)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 40 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42799

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P22816

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P22816

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q4V6U8

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P22816

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0002922

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42799/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002922

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002922

FlyMine - An integrated database for Drosophila genomics

FBgn0002922

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10250

MARRVEL_MODEL - MARRVEL (model organism gene)

42799

Linkouts

BioGRID - A database of protein and genetic interactions.

67737

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0002922

DroID - A comprehensive database of gene and protein interactions.

FBgn0002922

DRSC - Results frm RNAi screens

FBgn0002922

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

nau_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002922

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002922

Flygut - An atlas of the Drosophila adult midgut

FBgn0002922

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0002922

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015855

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

42799

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Myogenesis

References (167)

The following external sites may use different assemblies or annotations than FlyBase.

-0.66

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