FlyBase Gene Report: Dmel\sad

General Information

Symbol

Dmel\sad

Species

D. melanogaster

Name

shadow

Annotation Symbol

CG14728

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003312

Gene Model Status

Stock Availability

5 publicly available

Gene Summary

shadow (sad) encodes a cytochrome P450 involved in ecdysteroid biosynthesis. It shows a mitochondrial localization and catalyzes the addition of a hydroxyl group to the 2 carbon of the cholesterol ring. sad mutants fail to undergo head involution, dorsal closure or to secrete cuticle. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

Cyp315a1

Key Links

Genomic Location

Cytogenetic map

Sequence location

3R:11,877,235..11,879,754 [+]

Recombination map

3-51

RefSeq locus

NT_033777 REGION:11877235..11879754

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables ecdysteroid 2-hydroxylase activity

inferred from direct assay

(Warren et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables heme binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables iron ion binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in central nervous system development

inferred from mutant phenotype

(Giesen et al., 2003)

involved_in dorsal closure

inferred from mutant phenotype

(Giesen et al., 2003)

involved_in ecdysone biosynthetic process

inferred from direct assay

(Warren et al., 2002)

involved_in head involution

inferred from mutant phenotype

(Giesen et al., 2003)

involved_in midgut development

inferred from mutant phenotype

(Giesen et al., 2003)

involved_in motor neuron axon guidance

inferred from mutant phenotype

(Wright et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in ecdysone biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN004688505

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in mitochondrion

inferred from direct assay

(Talamillo et al., 2008, Petryk et al., 2003)

located_in nucleus

inferred from direct assay

(Talamillo et al., 2008)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

CYTOCHROME P450 - MITO CLAN

Protein Family (UniProt)

Belongs to the cytochrome P450 family. (Q9VGH1)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

shadow (sad) encodes a cytochrome P450 involved in ecdysteroid biosynthesis. It shows a mitochondrial localization and catalyzes the addition of a hydroxyl group to the 2 carbon of the cholesterol ring. sad mutants fail to undergo head involution, dorsal closure or to secrete cuticle. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

CYTOCHROME P450 - MITO CLAN -: Cytochrome P450, or CYP genes, constitute one of the largest families of genes, with representatives in virtually all living organisms. P450 enzymes are heme-thiolate proteins best known for their monooxygenase role, catalyzing the transfer of one atom of molecular oxygen to a substrate and reducing the other to water. The MITO clan includes D. melanogaster CYP12, 49, 301, 302, 314 and 315 family genes. Members are localized to mitochondria and at least some are linked to the ecdysteroid metabolism pathway. (Adapted from FBrf0248421.)

Protein Function (UniProtKB)

Required for CNS development: midline glial cells. Involved in the metabolism of insect hormones: responsible for ecdysteroid C2-hydroxylase activity. May be involved in the breakdown of synthetic insecticides.

(UniProt, Q9VGH1)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

sad: shadow (C. Nusslein-Volhard)

Embryonic lethal; no differentiation of cuticle or head skeleton; posterior of embryo condensed.

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\sad for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0082475

1773

520

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0081949

59.6

520

8.98

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Q9VGH1

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sad using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

sad_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

prothoracic gland

(Chung et al., 2009)

third instar larval stage

prothoracic gland

(Chung et al., 2009)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

prothoracic gland

• mitochondrion

(Talamillo et al., 2008)

oogenesis stage S1 -- S6 & adult stage | female

(Ameku and Niwa, 2016)

(Ameku and Niwa, 2016)

adult stage | female & oogenesis stage S10

main body follicle cell

(Ameku and Niwa, 2016)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in mitochondrion

inferred from direct assay

(Talamillo et al., 2008, Petryk et al., 2003)

located_in nucleus

inferred from direct assay

(Talamillo et al., 2008)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\sad in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\sad

View exonic expression by tissue for Dmel\sad

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003312

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003312

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG14728-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0003312

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of sad

Transgenic constructs containing regulatory region of sad

Aberrations (Deficiencies and Duplications) ( 10 )

Inferred from experimentation ( 10 )

Gene not disrupted in

(Giesen et al., 2003, Tearle and Nusslein-Volhard, 1987)

(Giesen et al., 2003, Giesen, 2001.1.24)

Gene disrupted in

(Christensen et al., 2008.4.15)

(Giesen et al., 2003, Tearle and Nusslein-Volhard, 1987, Jurgens et al., 1984)

(Ryder and Roote, 2004.11.10)

(Ryder and Roote, 2004.11.10)

(Ryder, 2004.4.26)

(Jurgens et al., 1984)

(Jurgens et al., 1984)

(Harbecke and Lengyel, 1995)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage

lethal - all die during embryonic stage | recessive

sad^unspecified

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

sad^GD5635

viable, with Scer\GAL4^Mef2.PR

sad^GD5635

viable, with Scer\GAL4^pnr-MD237

sad^GD5635

Other Phenotypes

Allele

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PR

sad^GD5635

abnormal neuroanatomy (with Df(3R)Exel7310)

sad¹

some die during embryonic stage

some die during embryonic stage | recessive

sad^unspecified

Phenotype manifest in

Allele

axon (with Df(3R)Exel7310)

sad¹

cephalopharyngeal skeleton

embryo | dorsal closure stage

embryo | embryonic stage 14

sad^10D

embryo | posterior

embryonic/first instar larval cuticle

embryonic/larval foregut

sad^unspecified

embryonic/larval hindgut

sad^unspecified

embryonic/larval midgut

sad^unspecified

embryonic/larval neuron | ectopic

sad^unspecified

embryonic/larval tracheal system

sad^10D

embryonic epidermis

sad^unspecified

embryonic midgut constriction

sad^10D

embryonic ventral epidermis

sad^unspecified

sad^unspecified

larval longitudinal connective

sad^unspecified

larval ventral nerve cord

sad^unspecified

midline glial cell | ectopic

sad^unspecified

sad¹

presumptive embryonic/larval central nervous system

presumptive embryonic/larval peripheral nervous system

sad^unspecified

sensory neuron | embryonic stage

sad^unspecified

symmetrical commissure

sad^unspecified

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

6 of 15

Yes

Yes

5 of 15

No

No

5 of 15

No

No

5 of 15

No

No

2 of 15

No

No

2 of 15

No

Yes

2 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (8)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

5 of 15

Yes

No

5 of 15

Yes

No

5 of 15

Yes

Yes

3 of 15

No

Yes

2 of 15

No

No

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Rattus norvegicus (Norway rat) (11)

6 of 13

Yes

Yes

5 of 13

No

No

5 of 13

No

Yes

2 of 13

No

No

2 of 13

No

Yes

2 of 13

No

Yes

Rnor\LOC100909462

2 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

Rnor\LOC100910462

1 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (10)

5 of 12

Yes

Yes

5 of 12

Yes

Yes

Xtro\LOC100145331

5 of 12

Yes

Yes

4 of 12

No

Yes

4 of 12

No

Yes

2 of 12

No

Yes

2 of 12

No

Yes

Xtro\LOC100496109

2 of 12

No

Yes

Xtro\LOC100496967

1 of 12

No

No

Xtro\LOC101731070

1 of 12

No

Yes

Danio rerio (Zebrafish) (15)

6 of 15

Yes

Yes

6 of 15

Yes

Yes

6 of 15

Yes

Yes

Drer\zgc:136333

6 of 15

Yes

Yes

5 of 15

No

Yes

5 of 15

No

Yes

Drer\si:ch211-113j14.1

5 of 15

No

No

Drer\si:dkey-91i10.3

5 of 15

No

Yes

4 of 15

No

No

2 of 15

No

No

2 of 15

No

Yes

2 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (19)

2 of 15

Yes

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

Arabidopsis thaliana (thale-cress) (9)

1 of 9

Yes

No

1 of 9

Yes

No

1 of 9

Yes

No

1 of 9

Yes

No

1 of 9

Yes

Yes

1 of 9

Yes

No

1 of 9

Yes

No

1 of 9

Yes

Yes

1 of 9

Yes

No

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091908FT )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00009905

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506KP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11439

Mayetiola destructor

Hessian fly

Mdes\Mdes011353

Aedes aegypti

Yellow fever mosquito

Anopheles darlingi

American malaria mosquito

Adar\ADAC003254

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06KV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA005496

Danaus plexippus

Monarch butterfly

Dple\DPOGS207804-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL017551

Apis florea

Little honeybee

Aflo\2764106052

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0016b7

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004055

Dendroctonus ponderosae

Mountain pine beetle

Dendroctonus ponderosae

Mountain pine beetle

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM320260

Pediculus humanus

Human body louse

Phum\PHUM250970

Rhodnius prolixus

Kissing bug

Rpro\RPRC006417

Cimex lectularius

Bed bug

Clec\CLEC003857

Cimex lectularius

Bed bug

Clec\CLEC001507

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000973

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI083030

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009774

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_09391

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_14659

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X08ZN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR010715

Strigamia maritima

European centipede

Smar\SMAR007995

Strigamia maritima

European centipede

Smar\SMAR005452

Ixodes scapularis

Black-legged tick

Isca\ISCW004801

Ixodes scapularis

Black-legged tick

Isca\ISCW021866

Stegodyphus mimosarum

African social velvet spider

Smim\KFM63264.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM70608.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur06g05620

Tetranychus urticae

Two-spotted spider mite

Turt\tetur41g00080

Tetranychus urticae

Two-spotted spider mite

Turt\tetur36g00120

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_60760

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_325858

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_325857

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (37)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CYP27C1; cytochrome P450 family 27 subfamily C member 1

6 of 15

CYP24A1; cytochrome P450 family 24 subfamily A member 1

5 of 15

HYPERCALCEMIA, INFANTILE, 1; HCINF1

CYP27A1; cytochrome P450 family 27 subfamily A member 1

5 of 15

CEREBROTENDINOUS XANTHOMATOSIS; CTX

cerebrotendinous xanthomatosis

CYP27B1; cytochrome P450 family 27 subfamily B member 1

5 of 15

VITAMIN D HYDROXYLATION-DEFICIENT RICKETS, TYPE 1A; VDDR1A

rickets

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0003312

MIST (protein-protein) - An integrated Molecular Interaction Database

sad

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Insect hormone biosynthesis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3R

Recombination map

3-51

Cytogenetic map

Sequence location

3R:11,877,235..11,879,754 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

87A1-87A1

Limits computationally determined from genome sequence between P{EP}Lk6^EP886 and P{PZ}svp⁰⁷⁸⁴²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

86F-87A1

(Dunkov, 2000.6.29)

Experimentally Determined Recombination Data

Location

3-51

(FlyBase, 2016, Tearle and Nusslein-Volhard, 1987)

3-48.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

82941

y¹ sc^* v¹ sev²¹; P{TKO.GS05210}attP40

67352

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06457}attP40

2087

ru¹ h¹ Diap1¹ st¹ cu¹ sad¹ sr¹ e^s ca¹/TM6B, Tb¹

v41269

w¹¹¹⁸; P{GD5635}v41269

v41270

w¹¹¹⁸; P{GD5635}v41270

Genomic Clones (25)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LP13573

Other clones

DPiM_LP13573

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003312

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP13359

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003312

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44858

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: sad Cyp315a1

(Petryk, 2002.2.15)

Additional comments

Other Comments

sad encodes a 2-hydroxylase that catalyses the conversion of 2-deoxyecdysone to ecdysone in S2 cells.

(Warren et al., 2002)

Four EMS induced alleles have been identified in a screen for mutations affecting commissure formation in the CNS of the embryo.

(Hummel et al., 1999)

sad mutants display no differentiation of the head skeleton or cuticle.

(Jurgens et al., 1984)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 33 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44858

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VGH1

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003312

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003312

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

sad_1

Flygut - An atlas of the Drosophila adult midgut

FBgn0003312

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44858

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014274

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG14728

modMine - A data warehouse for the modENCODE project

FBgn0003312

Linkouts

DPiM - Drosophila Protein interaction map

FBgn0003312

DroID - A comprehensive database of gene and protein interactions.

FBgn0003312

DRSC - Results frm RNAi screens

FBgn0003312

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0003312

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG14728-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003312

FlyMine - An integrated database for Drosophila genomics

FBgn0003312

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Insect hormone biosynthesis

MIST (protein-protein) - An integrated Molecular Interaction Database

sad

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

315a1

(Nelson, 2001.2.5)

CG14728

(Neuert et al., 2017, Keleman et al., 2009.8.5, Perkins et al., 2009)

CYP315A1

(Seong et al., 2018, Chen and Li, 2007, Rewitz et al., 2007, Borras et al., 2003, Ranson et al., 2002)

Cyp315a1

(Scanlan et al., 2020, Komura-Kawa et al., 2015, Niwa et al., 2011, Rewitz et al., 2007, McGraw et al., 2004, Kasai and Tomita, 2003, Tijet et al., 2001)

Sad

(Liu et al., 2018, Ono et al., 2006)

sad

(Scanlan et al., 2020, Zeng et al., 2020, Tsurumi et al., 2019, Ignesti et al., 2018, Setiawan et al., 2018, Uryu et al., 2018, Zhang et al., 2018, Christesen et al., 2017, Neuert et al., 2017, Transgenic RNAi Project members, 2017-, Di Cara and King-Jones, 2016, Valzania et al., 2016, Borsos et al., 2015, Komura-Kawa et al., 2015, Xie et al., 2015, Danielsen et al., 2014, Good et al., 2014, Neuman et al., 2014, Vandersmissen et al., 2014, Deng and Kerppola, 2013, Kwon et al., 2013, Nfonsam et al., 2012, Shaik et al., 2012, Vamos and Boros, 2012, Napoletano et al., 2011, Niwa et al., 2011, Ou et al., 2011, Chavoshi et al., 2010, Nagoshi et al., 2010, Pankotai et al., 2010, Wright et al., 2010, Xiang et al., 2010, Christensen et al., 2008.4.15, Huang et al., 2008, Mieszczanek et al., 2008, Talamillo et al., 2008, Curtis et al., 2007, McBrayer et al., 2007, Ono et al., 2006)

Name Synonyms

Shadow

(Niwa and Niwa, 2016, Pahi et al., 2015)

cytochrome P450

(Chung et al., 2009)

ecdysteroid hydroxylase

(Curtis et al., 2007)

karmoisin ghost

shadow

(Christensen et al., 2020, Mossman et al., 2019, Di Cara and King-Jones, 2016, Ou et al., 2016, Wieschaus and Nüsslein-Volhard, 2016, Borsos et al., 2015, Vandersmissen et al., 2014, Zhan et al., 2013, Gáliková et al., 2011, Ou et al., 2011, Chavoshi et al., 2010, Pankotai et al., 2010, Wright et al., 2010, Xiang et al., 2010, Huang et al., 2008, Talamillo et al., 2008, McBrayer et al., 2007, Rewitz et al., 2007, Hummel et al., 1999)

Secondary FlyBase IDs

FBgn0037957

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (124)