FlyBase Gene Report: Dmel\Cyp6g1

General Information

Symbol

Dmel\Cyp6g1

Species

D. melanogaster

Name

Cytochrome P450 6g1

Annotation Symbol

CG8453

Feature Type

protein_coding_gene

FlyBase ID

FBgn0025454

Gene Model Status

Current

Stock Availability

6 publicly available

Gene Summary

Necessary and sufficient for resistance to insecticides DDT and imidacloprid. May be involved in the metabolism of insect hormones. (UniProt, Q9V674)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

DDT-R, 6g1, Rst(2)DDT, cytochrome P450

Key Links

Genomic Location

Cytogenetic map

48E7-48E7

Sequence location

2R:12,185,667..12,188,431 [+]

Recombination map

2-66

RefSeq locus

NT_033778 REGION:12185667..12188431

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables heme binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401, InterPro:IPR036396

(InterPro Project Members, 2004-)

enables iron ion binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401, InterPro:IPR017972, InterPro:IPR036396

(InterPro Project Members, 2004-)

enables monooxygenase activity

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401, InterPro:IPR036396

(InterPro Project Members, 2004-)

enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002401, InterPro:IPR017972, InterPro:IPR036396

(InterPro Project Members, 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

involved_in insecticide catabolic process

inferred from direct assay

(Joußen et al., 2010, Joußen et al., 2008)

involved_in response to DDT

inferred from mutant phenotype

(Schmidt et al., 2010, Daborn et al., 2007, Le Goff et al., 2003, Daborn et al., 2002)

involved_in response to insecticide

inferred from mutant phenotype

(Harrop et al., 2014, Daborn et al., 2007, Le Goff et al., 2003)

inferred from mutant phenotype

(Daborn et al., 2002, Daborn et al., 2001)

involved_in response to mercury ion

inferred from mutant phenotype

(Rand et al., 2012)

involved_in response to organophosphorus

inferred from mutant phenotype

(Le Goff et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in insecticide catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN001958763

(GO Reference Genome Project, 2011-)

involved_in response to DDT

inferred from biological aspect of ancestor with PANTHER:PTN001958763

(GO Reference Genome Project, 2011-)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

CYTOCHROME P450 - CYP3 CLAN

Protein Family (UniProt)

Belongs to the cytochrome P450 family. (Q9V674)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYTOCHROME P450 - CYP3 CLAN -: Cytochrome P450, or CYP genes, constitute one of the largest families of genes, with representatives in virtually all living organisms. P450 enzymes are heme-thiolate proteins best known for their monooxygenase role, catalyzing the transfer of one atom of molecular oxygen to a substrate and reducing the other to water. The CYP3 clan includes D. melanogaster CYP6, 9, 28, 308-310 and 317 family genes. Few CYP3 enzymes have been characterized, though many have been shown to metabolize xenobiotics and plant natural compounds. (Adapted from FBrf0248421.)

Protein Function (UniProtKB)

Necessary and sufficient for resistance to insecticides DDT and imidacloprid. May be involved in the metabolism of insect hormones.

(UniProt, Q9V674)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Rst(2)DDT: Resistance (2) DDT

Rst(2)DDT is a major dominant gene responsible for the phenotype of a line selectd for resistance to DDT. Median lethal dose of DDT for Rst(2)DDT lines is about 4000 ug/cc of medium; that for sensitive lines is 50-100 ug/cc. Also resistant to BHC (benzene hexachloride) and organophosphorus insecticides such as parathion and malathion. Median lethal dose of parathion is 2 ppm for resistant line and 0.08 ppm for sensitive. Sensitive to phenylthiourea (Ogita, 1958, Botyu-Kagaku 2: 188-204) and phenylthiocarbamide (Dauring and Sunner, 1971, Hereditas 68: 115-22). Shows maternal effect in that progeny of Rst(2)DDT/+ female crossed to +/+ male are more resistant that those of reciprocal cross. Larva more resistant than adult. RK3.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Cyp6g1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V674)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cyp6g1-RA

FBtr0087992

NM_136899

2061

524

Cyp6g1-RB

FBtr0339596

NM_001299406

2074

524

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Cyp6g1-PA

FBpp0087100

59.9

524

8.28

FBpp0308668

59.9

524

8.28

Q9V674

NP_001286335

AHN56133

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

524 aa isoforms: Cyp6g1-PA, Cyp6g1-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cyp6g1 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.52

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

(Arbeitman et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval midgut

Comment: m1-2, m6-9

(Chung et al., 2009)

embryonic/larval fat body

(Chung et al., 2009)

embryonic/larval Malpighian tubule

(Chung et al., 2009)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{Cyp6g1-GAL4.Cs}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval midgut

(Chung et al., 2007, Bogwitz et al., 2005)

embryonic/larval Malpighian tubule

(Chung et al., 2007, Bogwitz et al., 2005)

Reporter: P{Cyp6g1-GAL4.HR}

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval midgut

(Southon et al., 2013, Lye et al., 2012, Chung et al., 2007, Bogwitz et al., 2005)

embryonic/larval fat body

(Southon et al., 2013, Chung et al., 2007, Bogwitz et al., 2005)

embryonic/larval Malpighian tubule

(Southon et al., 2013, Chung et al., 2007, Bogwitz et al., 2005)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Cyp6g1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cyp6g1

View exonic expression by tissue for Dmel\Cyp6g1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025454

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36316/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025454

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025454

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025454

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8453

Flygut - An atlas of the Drosophila adult midgut

FBgn0025454

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cyp6g1

Transgenic constructs containing regulatory region of Cyp6g1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 1 )

Dp(2;3)GV-CH321-22N18

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Cyp6g1^DDT-R

viable, with Scer\GAL4^Act5C.PI

viable, with Scer\GAL4^Mef2.PR

Cyp6g1^GD1945

viable, with Scer\GAL4^pnr-MD237

Cyp6g1^GD1945

Sterility

Allele

fertile, with Scer\GAL4^Act5C.PI

Other Phenotypes

Allele

chemical resistant

chemical resistant, with Scer\GAL4^c754

Cyp6g1^UAS.cDa

chemical resistant, with Scer\GAL4^Cyp6g1.HR

chemical resistant, with Scer\GAL4^elav-C155

Cyp6g1^UAS.cDa

chemical resistant, with Scer\GAL4^hs.PU

Cyp6g1^UAS.cDa

chemical resistant, with Scer\GAL4^αTub84B.PL

Cyp6g1^UAS.cDa

chemical resistant | maternal effect

Cyp6g1^DDT-R

chemical sensitive

Cyp6g1^DDT-R

chemical sensitive, with Scer\GAL4^Act5C.PI

chemical sensitive, with Scer\GAL4^c754

Cyp6g1^GD1945

flightless, with Scer\GAL4^Mef2.PR

Cyp6g1^GD1945

increased fecundity | dominant | female

Cyp6g1^DDT-R

increased rate of larval locomotory behavior, with Scer\GAL4^Cyp6g1.HR

Cyp6g1^UAS.cGa

Phenotype manifest in

Allele

M band, with Scer\GAL4^Mef2.PR

Cyp6g1^GD1945

myofibril, with Scer\GAL4^Mef2.PR

Cyp6g1^GD1945

sarcomere, with Scer\GAL4^Mef2.PR

Cyp6g1^GD1945

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (26)

Hsap\CYP3A5

8 of 14

Yes

Hsap\CYP3A7-CYP3A51P

8 of 14

Yes

Hsap\CYP3A43

8 of 14

Yes

7 of 14

7 of 14

7 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (32)

Rnor\Cyp3a9

10 of 14

Yes

Rnor\Cyp3a18

10 of 14

Yes

Rnor\Cyp3a62

10 of 14

Yes

9 of 14

9 of 14

9 of 14

1 of 14

Yes

Rnor\Cyp3a73

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (37)

Mmus\Cyp3a11

7 of 14

Yes

Mmus\Cyp3a16

7 of 14

Yes

Mmus\Cyp3a25

7 of 14

Yes

Mmus\Cyp3a41a

7 of 14

Yes

Mmus\Cyp3a41b

7 of 14

Yes

Mmus\Cyp3a44

7 of 14

Yes

Mmus\Cyp3a57

7 of 14

Yes

Mmus\Cyp3a59

7 of 14

Yes

Mmus\Tbxas1

7 of 14

Yes

6 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (18)

Xtro\cyp3a4.1

6 of 13

Yes

Xtro\cyp3a4.2

6 of 13

Yes

Xtro\tbxas1

5 of 13

Xtro\LOC100487054

2 of 13

Xtro\cyp3a5

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (25)

Drer\cyp3c1

9 of 14

Yes

Drer\cyp3c3

9 of 14

Yes

Drer\cyp3c4

9 of 14

Yes

Drer\tbxas1

9 of 14

Yes

Drer\cyp3a65

8 of 14

Drer\cyp3c2

2 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-113j14.1

1 of 14

Drer\si:dkey-91i10.3

1 of 14

Drer\zgc:123299

1 of 14

Drer\zgc:136333

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (29)

Cele\cyp-25A1

11 of 14

Yes

Cele\cyp-25A2

11 of 14

Yes

Cele\cyp-25A3

11 of 14

Yes

Cele\cyp-25A4

11 of 14

Yes

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

8 of 14

7 of 14

5 of 14

4 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (70)

Agam\AgaP_AGAP010961

12 of 12

Yes

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

6 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

4 of 12

3 of 12

3 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (37)

Atha\CYP711A1

9 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\ERG11

2 of 13

Yes

Scer\DIT2

1 of 13

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\erg5

3 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Cyp6g1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (62)

12 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

11 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

10 of 13

9 of 13

9 of 13

9 of 13

9 of 13

9 of 13

9 of 13

8 of 13

8 of 13

7 of 13

7 of 13

7 of 13

6 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

dib

1 of 13

sad

1 of 13

shd

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CYP3A5; cytochrome P450 family 3 subfamily A member 5

model of essential hypertension

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CYP3A5; cytochrome P450 family 3 subfamily A member 5

8 of 14

605325

HYPERTENSION, ESSENTIAL

essential hypertension

CYP3A7-CYP3A51P; CYP3A7-CYP3A51P readthrough

8 of 14

CYP3A43; cytochrome P450 family 3 subfamily A member 43

8 of 14

606534

CYP3A4; cytochrome P450 family 3 subfamily A member 4

7 of 14

124010

VITAMIN D-DEPENDENT RICKETS, TYPE 3; VDDR3

CYP3A7; cytochrome P450 family 3 subfamily A member 7

7 of 14

605340

TBXAS1; thromboxane A synthase 1

7 of 14

274180

GHOSAL HEMATODIAPHYSEAL DYSPLASIA; GHDD

Ghosal hematodiaphyseal syndrome

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-RNA

Physical Interaction

Assay

References

Cyp6g1 - mir-310

luminiscence technology

(Seong et al., 2020)

Cyp6g1 - mir-311

luminiscence technology

(Seong et al., 2020)

Cyp6g1 - mir-312

luminiscence technology

(Seong et al., 2020)

Cyp6g1 - mir-313

luminiscence technology

(Seong et al., 2020)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0025454

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-66

Cytogenetic map

48E7-48E7

Sequence location

2R:12,185,667..12,188,431 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

48E7-48E7

Limits computationally determined from genome sequence between P{PZ}SmD3⁰²⁸³³ and P{PZ}Cam⁰³⁹⁰⁹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

48E12-48F1

(Dunkov, 2000.6.29)

Experimentally Determined Recombination Data

Location

2-66

(FlyBase, 2016)

2-67.4

(Comeron et al., 2012)

2-65.0 +/- 0.55

(Daborn et al., 2001)

2-65

(Pacella Norman et al., 1996)

2-56.2

(Dapkus, 1992)

2-64.5-66

Left of (cM)

(Daborn et al., 2001)

Right of (cM)

(Daborn et al., 2001)

Notes

2-56.2 is the map position of DDT resistance in the 91R strain, assuming a single locus is involved. The 95% confidence interval is 2-55.1 to 2-56.7.

(Dapkus, 1992)

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

27863

w¹¹¹⁸; Mi{ET1}Cyp6g1^MB11394

41547

y¹ w^*; Mi{MIC}Cyp6g1^MI07054/CyO

42006

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02407}attP40

8453R-3

v104171

v4616

w¹¹¹⁸; P{GD1945}v4616

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (43)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI07613

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0025454

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0025454

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Festucci-Buselli et al., 2005)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

There is Cyp6g1 copy number variation and transposable element complexity in the Cyp6g1 region in the RK146 strain.

(Schmidt et al., 2010)

High expression of the Cyp6g1 gene does not necessarily confer DDT resistance in D.melanogaster.

(Kuruganti et al., 2007)

Overtranscription of the Cyp6g1 gene alone is necessary and sufficient for resistance to DDT.

(Daborn et al., 2002)

GH03082 is a chimeric cDNA clone containing two cDNAs in a tail-to-tail orientation that correspond to hoip (5' clone, mapped at 30C) and Cyp6g1 (GenBank accession number AF083946; 3' clone, mapped at 48F).

(Prokopenko et al., 2000)

The "RalDDT^R" strain (FBrf0052775) is highly resistant to DDT (and pyrethroid) by (mainly) cytochrome-P450-dependent mixed-function oxidases. FBrf0087658 studies the metabolism of diazinon in this strain in comparison to Canton-S.

(Pisani-Borg et al., 1996)

Relationship to Other Genes

Source for database merge of

Source for merge of: Cyp6g1 Rst(2)DDT

(Daborn et al., 2002)

Source for merge of: Cyp6g1 anon-WO03025223.16 anon-WO03025223.17

(FlyBase, 1996-)

Additional comments

FlyBase curator comment: the relationship between the "Rst(2)DDT-WIS1", "Cyp6g1", "Rst(2)DDT-EMS1" and "Rst(2)DDT-EMS2" genes is unclear. The "Rst(2)DDT-WIS1^WIS1", "Cyp6g1^DDT-R", "Rst(2)DDT-EMS1^EMS1" and "Rst(2)DDT-EMS2^EMS2" DDT resistant strains all map to the same region of the genome, which contains a cluster of P450 genes, but it has not been established whether or not they represent lesions in the same gene.

(Daborn et al., 2001)

Source for merge of Cyp6g1 anon-WO03025223.16 anon-WO03025223.17 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Possible to select new resistant alleles by growing the offspring of irradiated wild-type flies on parathion (Kikkawa, 1968). DDT resistance in the resistant 91R population mapped by Dapkus (1992) to 2-56.2 (95% confidence limit, 2-55.1 to 2-56.2), assuming a single locus. In other studies, with other strains, DDT resistance shown to be due to more than one locus (Crow 1954; Crow, 1957; King and Somme, 1958; Dapkus and Merrell, 1977). The relationship between Rst(2)DDT and Cyp6a2 is unclear.

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (25)

Reported As

Symbol Synonym

6g1

(Kasai and Tomita, 2003, Nelson, 2001.2.5)

CG8453

(Çiçek et al., 2016, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Panhuis and Swanson, 2006, Arbeitman et al., 2004, Jin et al., 2001)

CYP6G1

(Nirusimhan et al., 2022, Li et al., 2015, Harrop et al., 2014, Li et al., 2012, Jones et al., 2010, Hooven et al., 2009, Chen and Li, 2007)

CYP6g1

(Chen et al., 2022, Mahapatra et al., 2010)

Cyp6-like

Cyp6G1

(Kuzin et al., 2014, Li et al., 2008)

Cyp6g1

(He et al., 2025, Karageorgi et al., 2025, Manica-Cattani et al., 2025, Martelli et al., 2025, Serebrovska et al., 2025, Glaser-Schmitt et al., 2024, Tsuji et al., 2024, Xin et al., 2024, Ffrench-Constant, 2023, Fisher et al., 2023, Santos-Cruz et al., 2023, Liu et al., 2022, Amichot and Tarès, 2021, Garud et al., 2021, Anholt, 2020, Cohen et al., 2020, Douris et al., 2020, Haudry et al., 2020, Moschetti et al., 2020, Pu et al., 2020, Scanlan et al., 2020, Seong et al., 2020, Chakraborty et al., 2019, He et al., 2019, Zhang and Zhang, 2019, Akishina et al., 2018, Hammer et al., 2018, Perry and Batterham, 2018, Seong et al., 2018, Signor et al., 2018, Akishina et al., 2017, Denecke et al., 2017, Le Goff and Hilliou, 2017, Seong et al., 2017, Afschar et al., 2016, Cardoso-Moreira et al., 2016, Garud and Petrov, 2016, Hawkes et al., 2016, Denecke et al., 2015, Gellatly et al., 2015, Hales et al., 2015, Sun et al., 2015, Chatterjee et al., 2014, Harrop et al., 2014, Harrop et al., 2014, Hoi et al., 2014, Wan et al., 2014, Cheesman et al., 2013, Kalajdzic et al., 2013, Catalán et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Kalajdzic et al., 2012, Rand et al., 2012, Shah et al., 2012, Sztal et al., 2012, Chung et al., 2011, Müller et al., 2011, Niwa et al., 2011, Perry et al., 2011, Seong et al., 2011, Zhou et al., 2011, Beaver et al., 2010, Cridland and Thornton, 2010, Giraudo et al., 2010, Joußen et al., 2010, Kong et al., 2010, Morra et al., 2010, Narraway et al., 2010, Schmidt et al., 2010, Vázquez-Gómez et al., 2010, Carbone et al., 2009, Emerson et al., 2008, Hutter et al., 2008, Joußen et al., 2008, McCart and Ffrench-Constant, 2008, Turner et al., 2008, Chung et al., 2007, Daborn et al., 2007, FFrench-Constant, 2007, Kuruganti et al., 2007, ffrench-Constant et al., 2006, King-Jones et al., 2006, Panhuis and Swanson, 2006, Festucci-Buselli et al., 2005, Catania et al., 2004, Drnevich et al., 2004, Pyke et al., 2004)

Cyp6gl

(Ceriani et al., 2002)

DDT-R

(Rostant et al., 2017, Ffrench-Constant et al., 2016, Rostant et al., 2015, Good et al., 2014, ffrench-Constant et al., 2006)

DmCyp6g1

(Lin et al., 2023)

Dmel-Cyp6g1

(Harrop et al., 2014)

RDDT

(Berge et al., 1998)

(Pacella Norman et al., 1996)

RI^DDT

RI^II

Rst(2)DDT

(Daborn et al., 2001)

anon-WO03025223.16

anon-WO03025223.17

cyp6g1

(Zhang et al., 2025, Gao et al., 2022, Sharma et al., 2018, Gangishetti et al., 2009, Seugnet et al., 2009, Butcher et al., 2008, Zou et al., 2000)

Name Synonyms

CYP6-like

(Danielson, 1998.8.13)

Cyp6g1

(Cammarato et al., 2011, Pyke et al., 2004)

Cytochrome P450 6g1

Resistance to DDT

Resistance to Insecticide on chromosome 2

Resistance to Insecticide-DDT

cytochrome P450

(Chung et al., 2009, ffrench-Constant et al., 2006)

Secondary FlyBase IDs

FBgn0003288
FBgn0067801
FBgn0067802

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 58 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36316

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V674

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V674

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

B6IDY5

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V674

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025454

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36316/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025454

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025454

FlyMine - An integrated database for Drosophila genomics

FBgn0025454

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8453

MARRVEL_MODEL - MARRVEL (model organism gene)

36316

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0025454

DroID - A comprehensive database of gene and protein interactions.

FBgn0025454

DRSC - Results frm RNAi screens

FBgn0025454

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025454

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0025454

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8453

Flygut - An atlas of the Drosophila adult midgut

FBgn0025454

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

References (221)

The following external sites may use different assemblies or annotations than FlyBase.

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