FlyBase Gene Report: Dmel\vls

General Information

Symbol

Dmel\vls

Species

D. melanogaster

Name

valois

Annotation Symbol

CG10728

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003978

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

valois (vls) encodes a protein that functions as the substrate recognition platform for the protein arginine methyltransferase encoded by csul. Primary substrates of the products of csul and vls are the spliceosomal Sm proteins. The product of vls is also required for proper localization of the product of tud to the posterior pole of the oocyte during germ line specification. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Red Book
Interactive Fly

Also Known As

val, 38B.5

Key Links

Genomic Location

Cytogenetic map

38B2-38B2

Sequence location

2L:20,064,261..20,065,679 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:20064261..20065679

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in cellularization

inferred from high throughput mutant phenotype

(Schupbach and Wieschaus, 1989)

involved_in embryonic pattern specification

inferred from mutant phenotype

(Cavey et al., 2005)

involved_in pole plasm assembly

inferred from mutant phenotype

(Raff et al., 1990)

inferred from mutant phenotype

(Cavey et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in pole plasm assembly

non-traceable author statement

(Williamson and Lehmann, 1996)

traceable author statement

(Wylie, 1999)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Cavey et al., 2005)

located_in P granule

inferred from direct assay

(Anne and Mechler, 2005)

located_in pole plasm

inferred from direct assay

(Anne and Mechler, 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN005323416

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Protein Function (UniProtKB)

Involved in specific localization of cytoplasmic proteins during the formation of pole plasm. Required for synthesis and/or stability of oskar protein (osk) and localization of tudor (tud) in both the nuage and posterior pole of the oocyte. Required for normal posterior localization of osk in later stages of oogenesis and for posterior localization of the vasa (vas) protein during the entire process of pole plasm assembly. May act by regulating the complex that contains the arginine N-methyltransferase csul.

(UniProt, Q9VIP8)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

vls: valois (T. Schupbach)

exhibit a so-called "grandchildless-knirps" phenotype: all eggs lack polar granules and no pole cells are formed; most of the embryos show variable deletions of abdominal segments; whereby segment A4 is deleted most frequently, larger deletions may remove segments A2 through A7; in extreme cases anterior parts of segment A1 become fused to posterior parts of segment A8, but telson elements are always present and relatively normal. In addition, 80-90% of the embryos fail to cellularize normally at the blastoderm stage and die without forming cuticle, or only fragmented pieces of cuticle. Analysis of germline clones indicates that the mutation is germline autonomous (Schupbach and Wieschaus, 1986, Dev. Biol. 113: 443-48).

Summary (Interactive Fly)

scaffolding protein - the substrate recognition platform for the arginine methyltransferase Capsuleen - the primary substrates of Capsuleen and Valois are the spliceosomal Sm proteins - required for accumulation of high levels of Oskar protein, for posterior localization of Oskar in later stages of oogenesis and for posterior localization of the Vasa protein during pole plasm assembly

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\vls for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VIP8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

vls-RA

FBtr0081347

NM_136175

1310

367

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

vls-PA

FBpp0080879

40.6

367

6.73

Q9VIP8

NP_610019

AAF53868

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with csul and tud.

(UniProt, Q9VIP8)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\vls using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

vls_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.43

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

central brain anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | gap expression pattern

(Fisher et al., 2012)

trunk mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

organism | gap expression pattern

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium