FlyBase Gene Report: Dmel\Aralar

General Information

Symbol

Dmel\Aralar

Species

D. melanogaster

Name

mitochondrial aspartate/glutamate carrier

Annotation Symbol

CG2139

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028646

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

mitochondrial aspartate/glutamate carrier (Aralar) encodes a transmembrane transporter that catalyzes the calcium-dependent exchange of cytosolic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. It is involved in the malate-aspartate shuttle and gluconeogenesis. [Date last reviewed: 2024-09-19] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

aralar1, AGC

Key Links

Genomic Location

Cytogenetic map

99F4-99F5

Sequence location

3R:30,445,635..30,455,867 [-]

Recombination map

3-100

RefSeq locus

NT_033777 REGION:30445635..30455867

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (8 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables 3-sulfino-L-alanine: proton, glutamate antiporter activity

inferred from direct assay

(Lunetti et al., 2021)

enables aspartate:glutamate, proton antiporter activity

inferred from direct assay

(Lunetti et al., 2021)

enables gamma-aminobutyric acid:proton symporter activity

inferred from genetic interaction with FLYBASE:Cyfip; FB:FBgn0038320

(Kanellopoulos et al., 2020)

NOT enables gamma-aminobutyric acid transmembrane transporter activity

inferred from direct assay

(Porcelli et al., 2024)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables calcium ion binding

inferred from sequence or structural similarity with UniProtKB:O75746

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR002048

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR039800

(InterPro Project Members, 2004-)

enables identical protein binding

inferred from sequence or structural similarity with UniProtKB:O75746

(Gene Ontology Curators, 2002-)

enables L-aspartate transmembrane transporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000640306

(GO Reference Genome Project, 2011-)

enables L-glutamate transmembrane transporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000640306

(GO Reference Genome Project, 2011-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in gamma-aminobutyric acid transport

inferred from genetic interaction with FLYBASE:Cyfip; FB:FBgn0038320

(Kanellopoulos et al., 2020)

involved_in L-aspartate transmembrane transport

inferred from direct assay

(Lunetti et al., 2021)

involved_in L-glutamate transmembrane transport

inferred from direct assay

(Lunetti et al., 2021)

involved_in malate-aspartate shuttle

inferred from genetic interaction with UniProtKB:Q12482

(Lunetti et al., 2021)

NOT involved_in gamma-aminobutyric acid transport

inferred from direct assay

(Porcelli et al., 2024)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in aspartate transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000640306

(GO Reference Genome Project, 2011-)

involved_in gluconeogenesis

inferred from sequence or structural similarity with UniProtKB:Q9QXX4

(Gene Ontology Curators, 2002-)

involved_in L-glutamate transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000640306

(GO Reference Genome Project, 2011-)

involved_in malate-aspartate shuttle

inferred from biological aspect of ancestor with PANTHER:PTN000640306

(GO Reference Genome Project, 2011-)

involved_in mitochondrial calcium ion transmembrane transport

inferred from electronic annotation with InterPro:IPR039800

(InterPro Project Members, 2004-)

involved_in transmembrane transport

inferred from electronic annotation with InterPro:IPR002067

(InterPro Project Members, 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in mitochondrial inner membrane

inferred by curator from GO:0043490

(Lunetti et al., 2021)

inferred from biological aspect of ancestor with PANTHER:PTN000640304

(GO Reference Genome Project, 2011-)

located_in mitochondrial inner membrane

inferred from sequence or structural similarity with UniProtKB:O75746

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

SLC25 FAMILY OF MITOCHONDRIAL TRANSPORTERS

Pathway (FlyBase)

MALATE-ASPARTATE SHUTTLE

Protein Family (UniProt)

Belongs to the mitochondrial carrier (TC 2.A.29) family. (Q9VA73)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

gamma-aminobutyric acid:proton symporter activity
RHEA 28915: 3-sulfino-L-alanine: proton, glutamate antiporter activity
RHEA 70967: aspartate:glutamate, proton antiporter activity
RHEA 70783:

Summaries

Gene Snapshot

Gene Group (FlyBase)

SLC25 FAMILY OF MITOCHONDRIAL TRANSPORTERS -: The solute carrier family 25 (SLC25) or mitochondrial carrier family (MCF) members shuttle metabolites, nucleotides and cofactors across the inner mitochondrial membrane. (Adapted from FBrf0212481, FBrf0212949 and PMID:23266187).

Pathway (FlyBase)

MALATE-ASPARTATE SHUTTLE -: The malate-aspartate shuttle is composed of two cytosolic enzymes, two mitochondrial enzymes and two transporters. It is used to transport reducing equivalents from the cytosol to the mitochondrion, since cytosolic NADH cannot cross the inner mitochondrial membrane directly. The shuttle regenerates cytosolic NAD(+) to sustain cytosolic oxidative pathways such as glycolysis and provides reducing equivalents in the form of NADH for mitochondrial oxidative phosphorylation. (Adapted from PMID:38945121.)

Protein Function (UniProtKB)

Mitochondrial electrogenic aspartate/glutamate antiporter that favors efflux of aspartate and entry of glutamate and proton within the mitochondria as part of the malate-aspartate shuttle (PubMed:33497735). Also mediates the exchange of L-cysteinesulfinate (3-sulfino-L-alanine) for L-glutamate (PubMed:33497735). Necessary for gamma-aminobutyric acid (GABA) uptake in brain mitochondria in response to increased mitochondrial membrane polarization; does not possess detectable GABA transport activity but role may be indirect (PubMed:32200800, PubMed:38945283).

(UniProt, Q9VA73)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Aralar for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VA73)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Stop-codon suppression (UGA) postulated; FBrf0216884.

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Aralar-RA

FBtr0085694

NM_143538

2770

682

Aralar-RB

FBtr0085693

NM_170487

2945

679

Aralar-RC

FBtr0085692

NM_170485

2809

695

Aralar-RD

FBtr0085695

NM_170486

2340

682

Aralar-RE

FBtr0306586

NM_001260439

2845

707

Aralar-RF

FBtr0330290

NM_001276178

2770

694

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Aralar-PA

FBpp0085056

75.1

682

9.22

NP_651795

AAF57049

Aralar-PB

FBpp0085055

74.9

679

8.84

NP_733366

AAF57050

Aralar-PC

FBpp0085054

76.8

695

9.02

FBpp0085057

75.1

682

9.22

NP_733365

AAN14230

Aralar-PE

FBpp0297541

78.3

707

9.48

FBpp0303322

76.6

694

9.39

A0A0B4KHW3

NP_001263107

AGB96486

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

682 aa isoforms: Aralar-PA, Aralar-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer (via N-terminus).

(UniProt, Q9VA73)

Domain

Upon calcium binding, the EF-hand-containing regulatory N-terminal domain binds to the C-terminal domain, opening a vestibule which allows the substrates to be translocated through the carrier domain. In the absence of calcium, the linker loop domain may close the vestibule, which may prevent substrates from entering the carrier domain.

(UniProt, Q9VA73)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Aralar using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.10

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

primordial germ cell

(Fisher et al., 2012)

embryonic stage 7 -- 8

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

germline cell

(Fisher et al., 2012)

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic proventriculus

(Fisher et al., 2012)

gastric caecum

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Aralar in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Aralar

View exonic expression by tissue for Dmel\Aralar

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028646

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43616/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028646

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028646

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028646

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2139

Flygut - An atlas of the Drosophila adult midgut

FBgn0028646

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Aralar

Stages(s) 1-3

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 13 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Aralar

Transgenic constructs containing regulatory region of Aralar

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene partially disrupted in

Df(3R)slab-3

(Rohrbough et al., 2004)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal

Aralar^{MI07552-TG4.0}

Other Phenotypes

Allele

abnormal developmental rate

Aralar^{MI07552-TG4.0}

some die during P-stage

Aralar^{MI07552-TG4.0}

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (54)

Hsap\SLC25A12

14 of 14

Yes

Hsap\SLC25A13

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (37)

Rnor\Slc25a12

12 of 14

Yes

Rnor\Slc25a13

7 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (38)

Mmus\Slc25a12

13 of 14

Yes

Mmus\Slc25a13

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (33)

Xtro\slc25a12

10 of 13

Yes

Xtro\tspan15

9 of 13

Yes

Xtro\homez

7 of 13

Yes

Xtro\slc25a13

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (55)

Drer\slc25a12

13 of 14

Yes

Drer\slc25a13

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:dkey-150i13.2

1 of 14

Drer\si:dkey-178e17.1

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\slc25a38b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (37)

Cele\K02F3.2

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (36)

Agam\AgaP_AGAP000958

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (54)

Atha\ADNT1

2 of 13

Yes

Atha\AT1G72820

2 of 13

Yes

Atha\AT5G15640

2 of 13

Yes

Atha\AT5G26200

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (27)

Scer\AGC1

9 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Scer\MME1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (15)

Spom\SPAC4G8.08

1 of 12

Yes

Spom\SPAC4G9.20c

1 of 12

Yes

Spom\SPAC8C9.12c

1 of 12

Yes

Spom\SPAC12B10.09

1 of 12

Yes

Spom\SPAC19G12.05

1 of 12

Yes

Spom\SPAC227.03c

1 of 12

Yes

Spom\SPAC328.09

1 of 12

Yes

Spom\SPAC688.09

1 of 12

Yes

Spom\SPAC823.10c

1 of 12

Yes

Spom\SPBC12D12.05c

1 of 12

Yes

Spom\SPBC27B12.09c

1 of 12

Yes

Spom\SPBC29A3.11c

1 of 12

Yes

Spom\SPBC1271.11

1 of 12

Yes

Spom\SPBP23A10.06

1 of 12

Yes

Spom\SPCC1442.03

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Aralar. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (49)

GC1

5 of 13

GC2

5 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

sea

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Pef

1 of 13

Ucp4C

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Aralar^{MI07552-TG4.0}

model of neonatal-onset type II citrullinemia

CEA

(Martelli et al., 2024)

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

SLC25A12; solute carrier family 25 member 12

model of developmental and epileptic encephalopathy 39

IEA

(FlyBase, 2019-)

SLC25A13; solute carrier family 25 member 13

model of neonatal-onset type II citrullinemia

IEA

(FlyBase, 2019-)

model of adult-onset type II citrullinemia

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC25A12; solute carrier family 25 member 12

14 of 14

603667

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 39 WITH LEUKODYSTROPHY; DEE39

developmental and epileptic encephalopathy 39

SLC25A13; solute carrier family 25 member 13

12 of 14

603859

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Homodimer (via N-terminus).

(UniProt, Q9VA73 )

Linkouts

BioGRID - A database of protein and genetic interactions.

68470

DroID - A comprehensive database of gene and protein interactions.

FBgn0028646

MIST (protein-protein) - An integrated Molecular Interaction Database

43616

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

MALATE-ASPARTATE SHUTTLE

External Links

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-100

Cytogenetic map

99F4-99F5

Sequence location

3R:30,445,635..30,455,867 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

99F4-99F5

Limits computationally determined from genome sequence between P{PZ}ncd⁰⁵⁸⁸⁴ and P{lacW}l(3)s2500^s2500

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

99F-99F

(determined by in situ hybridisation)

(Del Arco et al., 2000)

Experimentally Determined Recombination Data

Location

3-100

(FlyBase, 2016)

3-98.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

43727

y¹ w^*; Mi{MIC}Aralar^MI07552

18846

w¹¹¹⁸; PBac{WH}Aralar^f05196/TM6B, Tb¹

79398

y¹ sc^* v¹ sev²¹; P{TKO.GS00685}attP40

79659

y¹ sc^* v¹ sev²¹; P{TOE.GS01219}attP40

56884

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04105}attP40

15960

y¹ w^67c23; P{EPgy2}Aralar^EY06229/TM3, Sb¹ Ser¹

97673

y¹ w^*; TI{CRIMIC.TG4.0}Aralar^{CR02896-TG4.0}/TM3, Sb¹ Ser¹

Kyoto

122065

w¹¹¹⁸; P{EP}Aralar^EP3675

2139R-1

2139R-9

v330528

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (82)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_LD35441

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0028646

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0028646

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lunetti et al., 2021)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Area matching Drosophila EST AA696927. This EST has sequence similarity to C. elegans ADP carrier protein gene. Probable intron in gene represented by EST AA696927.

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: aralar1 CG2139

(Bayraktaroglu, 2000.4.12)

Source for merge of: aralar1 BcDNA:GH07418

(FlyBase, 1996-)

Additional comments

Source for merge of aralar1 BcDNA:GH07418 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Aralar CG2139

(Del Arco et al., 2000)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

AGC

(Porcelli et al., 2024, Lunetti et al., 2021)

Aralar

(Mariano et al., 2023, Lavington et al., 2014)

Aralar1

(Porcelli et al., 2024, Pang et al., 2023, Vedelek et al., 2023, Kanellopoulos et al., 2020)

BcDNA:GH07418

CABP1

(Liu et al., 2018)

CG2139

(Ramnarine et al., 2022, Curcio et al., 2020, Meadows, 2018.4.4, Aradska et al., 2015, Jaspers et al., 2012, Cammarato et al., 2011, Müller et al., 2010, Ni et al., 2010.12.1, Labourier et al., 2002)

DmAralar

(Del Arco et al., 2000)

aralar

(Rohrbough et al., 2004)

Name Synonyms

Aralar1

(Cogni et al., 2015)

aralar1

(Jaspers et al., 2012, Cammarato et al., 2011)

mitochondrial aspartate/glutamate carrier

(Porcelli et al., 2024, Lunetti et al., 2021)

Secondary FlyBase IDs

FBgn0039772
FBgn0061423

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 77 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43616

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VA73

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VA73

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VA73

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028646

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43616/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028646

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028646

FlyMine - An integrated database for Drosophila genomics

FBgn0028646

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2139

MARRVEL_MODEL - MARRVEL (model organism gene)

43616

Linkouts

BioGRID - A database of protein and genetic interactions.

68470

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0028646

DroID - A comprehensive database of gene and protein interactions.

FBgn0028646

DRSC - Results frm RNAi screens

FBgn0028646

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028646

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0028646

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2139

Flygut - An atlas of the Drosophila adult midgut

FBgn0028646

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0028646

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC030731

MIST (protein-protein) - An integrated Molecular Interaction Database

43616

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (75)

The following external sites may use different assemblies or annotations than FlyBase.

-1.10

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