FlyBase Gene Report: Dmel\se

General Information

Symbol

Dmel\se

Species

D. melanogaster

Name

sepia

Annotation Symbol

CG6781

Feature Type

protein_coding_gene

FlyBase ID

FBgn0086348

Gene Model Status

Current

Stock Availability

34 publicly available

Enzyme Name (EC)

pyrimidodiazepine synthase (1.5.4.1)

glutathione dehydrogenase (ascorbate) (1.8.5.1)

glutathione transferase (2.5.1.18)

Gene Summary

sepia (se) is expressed in the adult eyes and its product is involved in drosopterin synthesis. [Date last reviewed: 2019-02-28] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

GSTO4

Key Links

Genomic Location

Cytogenetic map

66D5-66D5

Sequence location

3L:8,520,552..8,521,489 [+]

Recombination map

3-26

RefSeq locus

NT_037436 REGION:8520552..8521489

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables glutathione dehydrogenase (ascorbate) activity

inferred from direct assay

(Kim et al., 2006)

enables glutathione transferase activity

inferred from direct assay

(Saisawang et al., 2012, Kim et al., 2006)

enables protein homodimerization activity

inferred from physical interaction with FLYBASE:se; FB:FBgn0086348

(Kim et al., 2006)

enables pyrimidodiazepine synthase activity

inferred from direct assay

(Kim et al., 2006)

inferred from genetic interaction with FLYBASE:se; FB:FBgn0086348

(Kim et al., 2006)

inferred from mutant phenotype

(Wiederrecht and Brown, 1984)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables glutathione dehydrogenase (ascorbate) activity

inferred from biological aspect of ancestor with PANTHER:PTN000883725

(GO Reference Genome Project, 2011-)

enables glutathione transferase activity

inferred from electronic annotation with InterPro:IPR005442

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000883725

(GO Reference Genome Project, 2011-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in eye pigment biosynthetic process

inferred from genetic interaction with FLYBASE:se; FB:FBgn0086348

(Kim et al., 2006)

involved_in glutathione metabolic process

inferred from direct assay

(Saisawang et al., 2012)

involved_in pteridine biosynthetic process

inferred from direct assay

(Kim et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in glutathione metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000883725

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN005192534

(GO Reference Genome Project, 2011-)

located_in cytoplasm

inferred from sequence or structural similarity with RGD:70952

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR005442

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

SOLUBLE GLUTATHIONE S-TRANSFERASES

UNCLASSIFIED CH-NH OXIDOREDUCTASES

DEHYDROASCORBATE REDUCTASES

Protein Family (UniProt)

Belongs to the GST superfamily. Omega family. (Q9VSL3)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

glutathione transferase activity

RX + glutathione = an S-substituted glutathione + a halide anion + H(+) (2.5.1.18)

RHEA 16437: pyrimidodiazepine synthase activity

2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido[4,5-b][1,4]diazepin-4-one + glutathione disulfide + H2O = 6-pyruvoyl-5,6,7,8-tetrahydropterin + 2 glutathione (1.5.4.1)

RHEA 10720: glutathione dehydrogenase (ascorbate) activity

L-dehydroascorbate + 2 glutathione = glutathione disulfide + L-ascorbate (1.8.5.1)

RHEA 24424:

Summaries

Gene Snapshot

sepia (se) is expressed in the adult eyes and its product is involved in drosopterin synthesis. [Date last reviewed: 2019-02-28]

Gene Group (FlyBase)

SOLUBLE GLUTATHIONE S-TRANSFERASES -: Glutathione transferases (glutathione S-transferases, GST) catalyze nucleophilic attack by reduced glutathione on nonpolar compounds that contain an electrophilic carbon, nitrogen, or sulfur atom. At least 14 classes of soluble GSTs have been identified in prokaryotes and eukaryotes: Theta, Omega, Sigma and Zeta exist in all eukaryotes. D. melanogaster has two additional classes, Delta and Epsilon. In D. mel, the class is indicated by the letter after the Gst prefix. (Adapted from FBrf0217315 and PMID:15822171).
UNCLASSIFIED CH-NH OXIDOREDUCTASES -: This group comprises CH-NH oxidoreductases that do not classify under other groups in FlyBase.
DEHYDROASCORBATE REDUCTASES -: Dehydroascorbate reductases are part of the glutathione S-transferase superfamily and catalyze the glutathione (GSH)-dependent reduction of dehydroascorbic acid to ascorbic acid (ascorbate). The ability of ascorbate to donate electrons enables it to act as a free radical scavenger and to reduce higher oxidation states of iron to Fe[2+]. The iron reducing activity of ascorbate maintains the reactive centre Fe[2+] of 2-oxoglutarate-dependent dioxygenases thus preventing inactivation. (Adapted from PMID:29567393.)

Protein Function (UniProtKB)

Mediates the conversion of 2-amino-4-oxo-6-pyruvoyl-5,6,7,8-tetrahydropteridine (6-PTP; also named 6-pyruvoyltetrahydropterin) to 2-amino-6-acetyl-3,7,8,9-tetrahydro-3H-pyrimido(4,5-b)[1, 4]diazepin-4-one (pyrimidodiazepine or PDA), a key intermediate in red eye pigment drosopterin biosynthesis.

(UniProt, Q9VSL3)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

se: sepia

Eye color brown at eclosion, darkening to sepia, and becoming black with age. Thought to be the structural gene for the enzyme PDA synthetase which catalyzes the conversion of 6-pyruvoyltetrahydropterin to 2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido [4, 5, 6]-[1, 4] diazepine (=PDA), which is a precursor of the red drosopterin pigments. The enzyme has been partially purified and has a molecular weight of 48,000 daltons (Wiederrecht and Brown, 1984, J. Biol. Chem. 259: 14121-27). Pigment of ocelli normal. Chromatographically, se eyes characterized by having no red pigment [e.g. drosopterin and isodrosopterin (McKay, 1972, DIS 48: 62)] and an accumulation of yellow pigment (Hadorn and Mitchell, 1951, Proc. Nat. Acad. Sci. USA 37: 650-65; Ziegler-Gunder and Hadorn, 1958, Z. Vererbungsl. 89: 235-45). Three yellow pigments identified are 6- derivatives of 2-amino-4-hydroxy-7,8-dihydropteridine; the most abundant is sepiapterin, the 6-lactyl derivative; minor species are isosepiapterin (Viscontini and Mohlmann, 1959, Helv. Chim. Acta 42: 836-41; Forrest, VanBaalen, and Myers, 1959, Arch. Biochem. Biophys. 83: 508-20) and neosepiapterin (Takikawa, 1973, DIS 50: 158; Sugiura, Takikawa, Tsusue, and Goto, 1973, Bull. Chem. Soc. Jpn. 46: 3312-13), which are the 6-proprionyl and 6-acetyl derivatives respectively; the latter compound shown to be D-erythro-neopterin by Katoh and Arai (1974, DIS 51: 70). Other pteridines present in greater-than-normal amounts [e.g. isoxanthopterin II, xanthopterin I, and biopterin (McKay)]. Eye color autonomous in se eye disks transplanted into wildtype hosts (Beadle and Ephrussi, 1936, Genetics 21: 230). pn se flies have 10% amount of sepiapterin of se alone (Lifschytz and Falk, 1969, Genet. Res. 14: 53-61); dor se displays reduced viability (Lucchesi, 1968, Genetics 59: 37-44). RK1.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\se for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VSL3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

se-RA

FBtr0076623

NM_139978

825

243

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

se-PA

FBpp0076349

28.4

243

5.47

Q9VSL3

NP_648235

AAF50405

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q9VSL3)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9VSL3

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\se using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

se_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.53

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\se in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\se

View exonic expression by tissue for Dmel\se

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086348

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38973/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086348

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086348

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086348

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6781

Flygut - An atlas of the Drosophila adult midgut

FBgn0086348

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 23 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 20 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of se

Transgenic constructs containing regulatory region of se

Aberrations (Deficiencies and Duplications) ( 5 )

Inferred from experimentation ( 5 )

Gene not duplicated in

Ts(1Rt;3Lt)102

Gene duplicated in

Dp(3;3)h^M3

(Ingham et al., 1985)

Dp(3;4)hry⁺44

(Ingham et al., 1985)

Gene not disrupted in

Df(3L)hry-i22

(Ingham et al., 1985)

Gene disrupted in

Df(3L)se^pal

(Fanti et al., 2017)

Gene partially disrupted in

Df(3L)se^pal

(Fanti et al., 2017)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

se^41d17

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

se^GD10634

viable, with Scer\GAL4^pnr-MD237

se^GD10634

Other Phenotypes

Allele

abnormal eye color

visible | recessive

Phenotype manifest in

Allele

pigment cell

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (18)

Hsap\GSTO1

13 of 14

Yes

Hsap\GSTO2

13 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Gsto1

13 of 14

Yes

Rnor\Gsto2

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (12)

Mmus\Gsto1

13 of 14

Yes

Mmus\Gsto2

12 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (14)

Xtro\gsto1

10 of 13

Yes

Xtro\LOC100498493

8 of 13

Yes

Xtro\gsto2

6 of 13

Yes

Xtro\nkx2-5

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\stk32a

1 of 13

Danio rerio (Zebrafish) (11)

Drer\gsto1

13 of 14

Yes

Drer\gsto2

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (20)

Cele\gsto-2

12 of 14

Yes

Cele\gsto-3

12 of 14

Yes

Cele\C02D5.4

11 of 14

Cele\gst-44

11 of 14

Cele\gsto-1

11 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (31)

Agam\GSTO1

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (54)

Atha\GSTU6

8 of 13

Yes

Atha\GSTU1

7 of 13

Yes

Atha\GSTU2

7 of 13

Yes

Atha\GSTU3

7 of 13

Yes

Atha\GSTU4

7 of 13

Yes

Atha\GSTU5

7 of 13

Yes

Atha\GSTU7

7 of 13

Yes

Atha\GSTU8

7 of 13

Yes

Atha\GSTU19

7 of 13

Yes

Atha\GSTU20

7 of 13

Yes

Atha\GSTU21

7 of 13

Yes

Atha\GSTU22

7 of 13

Yes

Atha\GSTU23

7 of 13

Yes

Atha\GSTU24

7 of 13

Yes

Atha\GSTU25

7 of 13

Yes

Atha\GSTU26

7 of 13

Yes

Atha\GSTU28

7 of 13

Yes

Atha\ERD9

6 of 13

Yes

Atha\GSTU11

6 of 13

Yes

Atha\GSTU12

6 of 13

Yes

Atha\GSTU13

6 of 13

Yes

Atha\GSTU15

6 of 13

Yes

Atha\GSTU16

6 of 13

Yes

Atha\GSTU18

6 of 13

Yes

Atha\GSTU27

6 of 13

Yes

5 of 13

5 of 13

5 of 13

5 of 13

Yes

Atha\GSTU10

5 of 13

Yes

Atha\GSTU14

5 of 13

Yes

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\GTT2

2 of 13

Yes

Scer\CAM1

1 of 13

Scer\TEF4

1 of 13

Scer\YGR201C

1 of 13

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPCC1281.07c

1 of 12

Yes

Escherichia coli (enterobacterium) (3)

Ecol\sspA

3 of 11

Yes

Ecol\gstB

1 of 11

Ecol\yibF

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:se. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (38)

13 of 13

13 of 13

12 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GSTO1; glutathione S-transferase omega 1

13 of 14

GLUTATHIONE S-TRANSFERASE, OMEGA-1; GSTO1

GSTO2; glutathione S-transferase omega 2

13 of 14

GLUTATHIONE S-TRANSFERASE, OMEGA-2; GSTO2

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q9VSL3 )

Linkouts

BioGRID - A database of protein and genetic interactions.

64383

DroID - A comprehensive database of gene and protein interactions.

FBgn0086348

MIST (protein-protein) - An integrated Molecular Interaction Database

38973

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-26

Cytogenetic map

66D5-66D5

Sequence location

3L:8,520,552..8,521,489 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66D5-66D5

Limits computationally determined from genome sequence between P{PZ}Gug⁰³⁹²⁸&P{EP}CG6745^EP595b and P{lacW}foi^j8E8

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-26

(FlyBase, 2016)

3-20.1

(Comeron et al., 2012)

3-26.0

(University of Buenos Aires, 1951, Fernandez Gianotti, 1950, Bertani, 1947, Andres, 1945)

(Eloff, 1942)

Left of (cM)

Walker

(Furman and Kozhemyakina, 1991)

Sgs3

(Kokoza et al., 1982)

Right of (cM)

Notes

Stocks and Reagents

Stocks (34)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

1668

se¹

55131

se^Kiel

1669

se¹ e¹

4474

w^sey; se¹

93396

ap^56f; se¹

Kyoto

101750

se¹

101752

se^51j

106441

se¹ e¹

101751

se¹ e¹¹

106424

w^*; se¹ e¹

VDRC

v110210

P{KK102589}VIE-260B

v34227

w¹¹¹⁸ P{GD10634}v34227

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (12)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH04924

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0086348

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0086348

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Analysis of variance of developmental time and viability of pteridine pathway mutants in sf, se, Hn, dke and bw, indicated that viability of induced and natural population alleles is the same whereas developmental time tends to be longer for induced mutations as compared to natural population alleles.

(Millan and Najera, 1992)

se mutants are deficient in levels of 'quench spot' and drosopterins. 'Quench spot' is proposed to be a precursor of drosopterins and is formed from the sepiapterin synthase intermediate.

(Dorsett and Jacobson, 1982)

Thought to be the structural gene for the enzyme PDA synthetase which catalyzes the conversion of 6-pyruvoyltetrahydropterin to 2-amino-4-oxo-6-acetyl-7,8-dihydro-3H,9H-pyrimido^4,5,6-^1,4 diazepine (=PDA), which is a precursor of the red drosopterin pigments. The enzyme has been partially purified and has a molecular weight of 48,000 daltons (Wiederrecht and Brown, 1984). Pigment of ocelli normal. Chromatographically, se eyes characterized by having no red pigment <up>e.g. drosopterin and isodrosopterin (McKay, 1972)</up> and an accumulation of yellow pigment (Hadorn and Mitchell, 1951; Ziegler-Gunder and Hadorn, 1958). Three yellow pigments identified are 6- derivatives of 2-amino-4-hydroxy-7,8-dihydropteridine; the most abundant is sepiapterin, the 6-lactyl derivative; minor species are isosepiapterin (Viscontini and Mohlmann, 1959; Forrest, VanBaalen, and Myers, 1959) and neosepiapterin (Takikawa, 1973; Sugiura, Takikawa, Tsusue and Goto, 1973), which are the 6-proprionyl and 6-acetyl derivatives respectively; the latter compound shown to be D-erythro-neopterin by Katoh and Arai (1974). Other pteridines present in greater-than-normal amounts <up>e.g. isoxanthopterin II, xanthopterin I and biopterin (McKay)</up>. Eye color autonomous in se eye discs transplanted into wild-type hosts (Beadle and Ephrussi, 1936). pn se flies have 10% amount of sepiapterin of se alone (Lifschytz and Falk, 1969); dor se displays reduced viability (Lucchesi, 1968).

Relationship to Other Genes

Source for database merge of

Source for merge of: se CG6781

(Kim et al., 2006)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

FlyBase Curator comment: Note that (at least) three genes have been referred to as 'GstO1' (or a slight variant thereof) in the literature: CG6662 (in FBrf0217315), CG6673 (in FBrf0217543) and CG6781/se (in FBrf0191145). FlyBase has used 'GstO1' as the official symbol for CG6662 based on FBrf0217315 as this was the first peer-reviewed research paper to name the gene, and it presents a unified and systematic nomenclature for all Gst genes.

(FlyBase, 1992-)

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

CG6781

(Çiçek et al., 2016, Rosa-Ferreira et al., 2015, Kim et al., 2013, Saisawang et al., 2012, Keleman et al., 2009.8.5, Perkins et al., 2009, Walters et al., 2009, Li et al., 2008, Kim et al., 2007, Kim et al., 2006, Marco et al., 2004)

DmGSTO4

(Wongtrakul et al., 2014)

GSTO1

(Whitbread et al., 2005)

GSTO4

(Saisawang and Ketterman, 2014, Wongtrakul et al., 2014, Saisawang et al., 2012)

PDAS

(Ferré, 2024)

(Li et al., 2024)

Sepia

(Gama-Brambila et al., 2021)

cg6781

(Low et al., 2007)

dmgsto1

(Whitbread et al., 2005)

(Ferré, 2024, Baker et al., 2021, Hagen et al., 2021, Hall et al., 2021, Port et al., 2020, Port et al., 2020, Fanti et al., 2017, Transgenic RNAi Project members, 2017-, Port et al., 2015, Bischof and FlyORF project members, 2014.6.20, Reed, 2014.2.19, Senthilan et al., 2012, Walters et al., 2009, Elias et al., 2008, Kim et al., 2006)

sepia

(Fortuna et al., 2021)

Name Synonyms

CG6781

PDA Synthase

(Kim et al., 2013)

Sepia

(Kumimoto et al., 2013)

pyrimidodiazepine synthase

(Ferré, 2024)

sepia

(Ferré, 2024, Colpitts et al., 2017, Fanti et al., 2017, Enya et al., 2014, Reed, 2014.2.19, Kim et al., 2013, Pandey et al., 2013, Vahl et al., 2013, Maclellan et al., 2012, Walters et al., 2009, Kim et al., 2007, Kim et al., 2006)

Secondary FlyBase IDs

FBgn0003351
FBgn0035905

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 54 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38973

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VSL3

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VSL3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VSL3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0086348

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38973/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0086348

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0086348

FlyMine - An integrated database for Drosophila genomics

FBgn0086348

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6781

MARRVEL_MODEL - MARRVEL (model organism gene)

38973

Linkouts

BioGRID - A database of protein and genetic interactions.

64383

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0086348

DroID - A comprehensive database of gene and protein interactions.

FBgn0086348

DRSC - Results frm RNAi screens

FBgn0086348

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

se_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0086348

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0086348

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6781

Flygut - An atlas of the Drosophila adult midgut

FBgn0086348

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0086348

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

38973

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (120)

The following external sites may use different assemblies or annotations than FlyBase.

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