FlyBase Gene Report: Dmel\mld

General Information

Symbol

Dmel\mld

Species

D. melanogaster

Name

molting defective

Annotation Symbol

CG34100

Feature Type

protein_coding_gene

FlyBase ID

FBgn0263490

Gene Model Status

Current

Stock Availability

46 publicly available

Gene Summary

molting defective (mld) encodes a nuclear zinc finger protein required for ecdysone biosynthesis. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

DTS-3, l(3)3^DTS

Key Links

Genomic Location

Cytogenetic map

96A9-96A12

Sequence location

3R:24,585,653..24,623,899 [+]

Recombination map

3-85

RefSeq locus

NT_033777 REGION:24585653..24623899

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables transcription cis-regulatory region binding

inferred from biological aspect of ancestor with PANTHER:PTN009094639

(GO Reference Genome Project, 2011-)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR012934

(InterPro Project Members, 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in determination of adult lifespan

inferred from mutant phenotype

(Simon et al., 2003)

involved_in ecdysone biosynthetic process

inferred from mutant phenotype

(Neubueser et al., 2005)

involved_in long-term memory

inferred from mutant phenotype

(Ishimoto et al., 2009)

involved_in positive regulation of circadian sleep/wake cycle, sleep

inferred from mutant phenotype

(Ishimoto and Kitamoto, 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN009094639

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Talamillo et al., 2008, Neubueser et al., 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN009094639

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred from electronic annotation with InterPro:IPR012934

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

molting defective (mld) encodes a nuclear zinc finger protein required for ecdysone biosynthesis. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

C2H2 ZINC FINGER TRANSCRIPTION FACTORS -: Zinc finger C2H2 transcription factors are sequence-specific DNA binding proteins that regulate transcription. They possess DNA-binding domains that are formed from repeated Cys₂His₂ zinc finger motifs. (Adapted from PMID:1835093, FBrf0220103 and FBrf0155739).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

l(3)DTS3

Heterozygous larvae raised at 29 arrested at the third lar- val instar for up to two weeks; exhibit grossly hypertrophied ring glands due to the growth of the prothoracic gland cells. The nuclei possess polytene chromosomes which are as large as those of salivary gland nuclei; their banding pattern is simi- lar but not identical to that of salivary gland polytene chro- mosomes, but the puffing patterns are quite different. Salivary gland chromosomes also hypertrophy and lack the nor- mal ecdysone-induced puffs. Chromosomes in both tissues respond to adminstration of ecdysone by producing the charac- teristic set of puffs (Holden and Ashburner, 1978, Chromosoma 68: 205-27). At 29 imaginal discs unable to evert in situ; however evert normally if cultured in the presence of ecdys- terone or in wild-type hosts. Implantation of a single wild- type ring gland rescues lethality (Holden, Walker, Maroy, Wat- son, White, and Gausz, 1986, Dev. Genet. 6: 153-62).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\mld for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0B4K7S6)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mld-RA

FBtr0111012

NM_001043289

6957

1918

mld-RC

FBtr0304912

NM_001043290

6954

1918

mld-RD

FBtr0304913

NM_001260360

7098

1965

mld-RE

FBtr0304914

NM_143016

7029

1943

mld-RF

FBtr0304915

NM_001260359

6891

1896

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

mld-PA

FBpp0110311

212.9

1918

6.05

FBpp0293451

212.9

1918

6.05

FBpp0293452

218.7

1965

6.20

FBpp0293453

216.3

1943

6.25

FBpp0293454

210.4

1896

6.11

A0A0B4K6J3

NP_001247288

AFH06606

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1918 aa isoforms: mld-PA, mld-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mld using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mld_1
mld_2

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.71

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

pole plasm

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium