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Gene Dmel\magu

General Information
Symbol	Dmel\magu	Species	D. melanogaster
Name	magu	Annotation symbol	CG2264
Feature type	protein_coding_gene	FlyBase ID	FBgn0262169
Gene Model Status	Current	Stock availability	12 publicly available
Also Known As	PENT
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	46D9-46E1	Sequence location	2R:5,940,046..5,951,217 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene magu is referred to in FlyBase by the symbol Dmel\magu (CG2264, FBgn0262169). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: heparan sulfate proteoglycan binding. There is experimental evidence that it is involved in the biological process: germ-line stem cell maintenance; regulation of BMP signaling pathway; determination of adult lifespan; positive regulation of BMP signaling pathway. 29 alleles are reported. The phenotypes of these alleles are annotated with: wing vein L5; testis; wing blade; wing vein. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Calcium-binding EF-hand; EF-Hand 1, calcium-binding site; EF-hand-like domain; Kazal domain; SPARC/Testican, calcium-binding domain; Thyroglobulin type-1. Gene sequence location is 2R:5940046..5951217.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 46D9-46E1   Limits computationally determined from genome sequence between P{lacW}Adamk13906&P{EP}Pka-R2EP2162 and P{PZ}14-3-3ζ07103
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\magu for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) magu-RA FBtr0088401  NM_165730   2994   613 magu-RB FBtr0088402  NM_136704   2838   613 magu-RD FBtr0088399  NM_165732   2710   613 magu-RF FBtr0113062  NM_001103784   2715   520
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein magu-PA   FBpp0087489   69.0   613   8.64     NP_724871   AAG22291 magu-PB   FBpp0087490   69.0   613   8.64     NP_610548   AAF58854 magu-PD   FBpp0087487   69.0   613   8.64     NP_724873   AAM71053 magu-PF   FBpp0111975   59.1   520   9.29     NP_001097254   AAM71054
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Thyroglobulin type-1 (IPR000716) EF-hand domain (IPR002048) Kazal domain (IPR002350) EF-hand-like domain (IPR011992) EF-Hand 1, calcium-binding site (IPR018247) SPARC/Testican, calcium-binding domain (IPR019577)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA695774   AA696274   AA696833   AA950777   AE013599 AAF58854   AE013599 AAG22291   AE013599 AAM71053   AE013599 AAM71054   AI109469   AI513738   AI515369   AQ073453   AW943055   AY058615 AAL13844   AY242076 AAO85264   BG633661   BG639211   BH809421   BI165224   BI170102   BI212287   BI215625   BI238477   BI483448   BI578570   BI580914   BP543316   BP543317   BP545615   BP545616   BP546415   BP548097   BP553169   BP553170   BP554155   BP554156   BP554676   BP554677   BT044202   CA806421   CO264591   CO308310   CO312273   CO313273   CO328960   CO329970   CZ471467   CZ471468   CZ472552   CZ475770   CZ477963   CZ478326   CZ480027   CZ484765   EC060530   EC075230   EC083855   EC089084   EC213562   EC225605   EC227852   ES689090   ES689581   ES689632   ES689839   ES690547   ES690556   ES690585   ES690600   GH953225   GH959269   GH984997
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	A4UZB1 Q0E9E0 Q86MK1 Q95TQ2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
colocalizes_with external side of plasma membrane inferred from direct assay (Vuilleumier et al., 2010) extracellular region inferred from direct assay (Vuilleumier et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    8 central nervous system, pupae P8    5 head, virgin 1-day female    7 head, virgin 4-day female    5 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    10 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    3 fat body, white prepupae    2 fat body, pupae P8    11 carcass, larvae L3 wandering    14 carcass, 1-day adult    12 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    37 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    8 central nervous system, pupae P8    5 head, virgin 1-day female    7 head, virgin 4-day female    5 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    10 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    3 fat body, white prepupae    2 fat body, pupae P8    11 carcass, larvae L3 wandering    14 carcass, 1-day adult    12 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female  (37) ovary, mated 4-day female  (30) testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    8 central nervous system, pupae P8    5 head, virgin 1-day female    7 head, virgin 4-day female    5 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    10 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    3 fat body, white prepupae    2 fat body, pupae P8    11 carcass, larvae L3 wandering    14 carcass, 1-day adult    12 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    37 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    8 central nervous system, pupae P8    5 head, virgin 1-day female    7 head, virgin 4-day female    5 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    10 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    3 fat body, white prepupae    2 fat body, pupae P8    11 carcass, larvae L3 wandering    14 carcass, 1-day adult    12 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    37 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    8 central nervous system, pupae P8    5 head, virgin 1-day female    7 head, virgin 4-day female    5 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    10 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    3 fat body, white prepupae    2 fat body, pupae P8    11 carcass, larvae L3 wandering    14 carcass, 1-day adult    12 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    37 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    8 central nervous system, pupae P8    5 head, virgin 1-day female    7 head, virgin 4-day female    5 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    10 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    3 fat body, white prepupae    2 fat body, pupae P8    11 carcass, larvae L3 wandering    14 carcass, 1-day adult    12 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female  (37) ovary, mated 4-day female  30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    8 central nervous system, pupae P8    5 head, virgin 1-day female    7 head, virgin 4-day female    5 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    10 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    3 fat body, white prepupae    2 fat body, pupae P8    11 carcass, larvae L3 wandering    14 carcass, 1-day adult    12 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    37 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    15 central nervous system, larvae L3    8 central nervous system, pupae P8    5 head, virgin 1-day female    7 head, virgin 4-day female    5 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    10 head, mated 4-day male    13 head, mated 20-day male    14 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    5 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    3 fat body, white prepupae    2 fat body, pupae P8    11 carcass, larvae L3 wandering    14 carcass, 1-day adult    12 carcass, 4-day adult    12 carcass, 20-day adult    9 ovary, virgin 4-day female    37 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    25 embryo 04-06hr    36 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    23 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    5 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    16 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    12 pupae 3d postWPP    12 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    17 adult male 30day    15 adult female 01day    26 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (46) embryo 02-04hr    25 embryo 04-06hr  (36) embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    23 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    5 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    16 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    12 pupae 3d postWPP    12 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    17 adult male 30day    15 adult female 01day  26 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    25 embryo 04-06hr    36 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    23 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    5 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    16 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    12 pupae 3d postWPP    12 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    17 adult male 30day    15 adult female 01day    26 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    25 embryo 04-06hr    36 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    23 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    5 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    16 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    12 pupae 3d postWPP    12 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    17 adult male 30day    15 adult female 01day    26 adult female 05day    25 adult female 30day    25 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    25 embryo 04-06hr    36 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    23 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    5 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    16 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    12 pupae 3d postWPP    12 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    17 adult male 30day    15 adult female 01day    26 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (46) embryo 02-04hr    25 embryo 04-06hr  (36) embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    23 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    5 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    16 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    12 pupae 3d postWPP    12 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    17 adult male 30day    15 adult female 01day  26 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    25 embryo 04-06hr    36 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    23 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    5 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    16 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    12 pupae 3d postWPP    12 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    17 adult male 30day    15 adult female 01day    26 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    46 embryo 02-04hr    25 embryo 04-06hr    36 embryo 06-08hr    21 embryo 08-10hr    21 embryo 10-12hr    23 embryo 12-14hr    10 embryo 14-16hr    12 embryo 16-18hr    10 embryo 18-20hr    7 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    4 larva L3 12hr old    5 larva L3 puffstage 1-2    13 larva L3 puffstage 3-6    18 larva L3 puffstage 7-9    16 white prepupae new    11 white prepupae 12hr    13 white prepupae 24hr    20 pupae 2d postWPP    12 pupae 3d postWPP    12 pupae 4d postWPP    19 adult male 01day    17 adult male 05day    17 adult male 30day    15 adult female 01day    26 adult female 05day    25 adult female 30day    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    3 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    17 embryonic S1    2 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    3 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    1 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    1 ovary fGS/OSS    74 ovary OSC    14 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    3 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    17 embryonic S1    2 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    3 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    1 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    1 ovary fGS/OSS  (74) ovary OSC    14 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    3 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    17 embryonic S1    2 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    3 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    1 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    1 ovary fGS/OSS    74 ovary OSC    14 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    3 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    17 embryonic S1    2 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    3 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    1 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    1 ovary fGS/OSS    74 ovary OSC    14 ovary OSS    21 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    3 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    17 embryonic S1    2 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    3 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    1 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    1 ovary fGS/OSS    74 ovary OSC    14 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    3 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    17 embryonic S1    2 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    3 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    1 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    1 ovary fGS/OSS  (74) ovary OSC    14 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    3 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    17 embryonic S1    2 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    3 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    1 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    1 ovary fGS/OSS    74 ovary OSC    14 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    3 embryonic 1182-4H    1 embryonic GM2    0 embryonic Kc167    17 embryonic S1    2 embryonic S3    1 leg disc CME L1    2 wing disc CME-W2    3 wing disc ML-DmD8    5 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    1 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    1 eye-antennal disc ML-DmD11    1 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    0 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    1 ovary fGS/OSS    74 ovary OSC    14 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  (27) Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  27 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    9 cold shock, 4-day adult    6 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    21 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    17 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    23 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable magud00269 maguKG02847b viable (with mague00439) maguf02256 viable (with maguf02256) mague00439 Other Phenotypes Allele decreased cell number | recessive magu2 long lived maguPdL maguTn10\tetO.cLa long lived, with Scer\GAL4D42, Tn10\tetRM2.alt.Scer\UAS.T:Hsim\VP16 maguTn10\tetO.cLa visible | recessive magu2 Phenotype manifest in Allele male germline stem cell | third instar larval stage magudel1 maguKG76 male germline stem cell | third instar larval stage (with mague00439) maguf02256 male germline stem cell | third instar larval stage (with maguf02256) mague00439 testis magudel1 maguKG76 testis (with mague00439) maguf02256 testis (with maguf02256) mague00439 wing blade magu2 wing blade, with Scer\GAL4dpp.blk1 maguScer\UAS.T:SV5\V5 wing vein (with mague00439) maguf02256 wing vein (with maguf02256) mague00439 wing vein L5 magu2
Classical Alleles ( 22 )
For All Classical Alleles Show
Allele of magu Class Mutagen Stocks Known lesion magud00225 P-element activity 1 -- magud00269 1 -- mague00053 piggyBac transposase 1 -- mague00439 piggyBac transposase 1 -- maguEP2277 P-element activity 1 -- maguf02256 piggyBac transposase 1 -- maguG12350 P-element activity 1 -- maguKG02847b P-element activity 1 -- maguMI00293 1 -- maguMI02087 1 -- magu2 0 -- maguA17 0 -- magud01992 P-element activity 0 -- magud06539 0 -- magud07494 P-element activity 0 -- magudel1 FLPase 0 Yes mague02201 piggyBac transposase 0 -- maguKG123 P-element activity 0 Yes maguKG166 P-element activity 0 Yes maguKG76 P-element activity 0 Yes maguKG862 P-element activity 0 Yes maguPdL 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of magu Class Mutagen Stocks Known lesion maguGL01223 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes maguKK108507 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes maguGD5297 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes maguScer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes maguScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes maguScer\UAS.T:SV5\V5 in vitro construct - regulatory fusion 0 Yes maguTn10\tetO.cLa in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(2R)pentA17 (Vuilleumier et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{TRiP.GL01223} NA P{UAS-magu.V} NA P{UAS-magu-GFP} NA P{UAS-magu-Myc} NA characterization construct P{tetO-magu.L} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}maguEP2277 NA P{EP}maguG12350 NA P{PdL}magu NA P{XP}magud00225 NA P{XP}magud00269 NA P{XP}magud01992 NA P{XP}magud06539 NA P{XP}magud07494 NA miscellaneous insertions Mi{MIC}maguMI00293 NA Mi{MIC}maguMI02087 NA P{SUPor-P}maguKG02847b NA PBac{RB}mague00053 NA PBac{RB}mague00439 NA PBac{RB}mague02201 NA PBac{WH}maguf02256 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
heparan sulfate proteoglycan binding inferred from direct assay (Vuilleumier et al., 2010)
Biological Process
CV term Evidence References
determination of adult lifespan inferred from mutant phenotype (Li and Tower, 2009) germ-line stem cell maintenance inferred from mutant phenotype (Zheng et al., 2011) positive regulation of BMP signaling pathway inferred from direct assay (Zheng et al., 2011) regulation of BMP signaling pathway inferred from mutant phenotype (Vuilleumier et al., 2010)
Cellular Component
CV term Evidence References
colocalizes_with external side of plasma membrane inferred from direct assay (Vuilleumier et al., 2010) extracellular region inferred from direct assay (Vuilleumier et al., 2010)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
calcium ion binding inferred from electronic annotation with InterPro:IPR002048, InterPro:IPR011992, InterPro:IPR019577 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
proteinaceous extracellular matrix inferred from electronic annotation with InterPro:IPR019577 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene (Vuilleumier et al., 2008)
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions magu allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0262169 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36048
Orthologs
OrthoDB Orthologs (35) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG602XBX Dipteran inclusive ortholog search EOG6BG94F Insect inclusive ortholog search EOG6GXDHF Arthropod inclusive ortholog search EOG69P8F3 Metazoa inclusive ortholog search EOG6KHFP8
Orthologs in Drosophila Species (EOG602XBX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\magu     Drosophila simulans   Dsim\GD10710 Y   Drosophila sechellia   Dsec\GM21178 Y   Drosophila erecta   Dere\GG24133 Y   Drosophila yakuba   Dyak\GE19332 Y   Drosophila ananassae   Dana\GF12013 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA15334 Y   Drosophila persimilis   Dper\GL11515 Y   Drosophila willistoni   Dwil\GK21959 Y   Drosophila virilis   Dvir\GJ19867 Y   Drosophila mojavensis   Dmoj\GI20098 Y   Drosophila grimshawi   Dgri\GH21645 Y
Orthologs in non-Drosophila Dipterans (EOG6BG94F)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012043   Anopheles gambiae Malaria mosquito  Agam\AGAP007489
Orthologs in non-Dipteran Insects (EOG6GXDHF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB53268   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG004704   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH20666   Atta cephalotes Leafcutter ant  Acep\ACEP17171   Atta cephalotes Leafcutter ant  Acep\ACEP17169   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19376   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19320   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19321   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19231   Linepithema humile Argentine ant  Lhum\LH19959   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18377   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18376   Solenopsis invicta Red fire ant  Sinv\SINV15835   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000745   Bombyx mori Silkmoth  Bmor\BGIBMGA000730   Pediculus humanus Human body louse  Phum\PHUM607640   Tribolium castaneum Red flour beetle  Tcas\TC010525
Orthologs in non-Insect Arthropods (EOG69P8F3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_194402   Ixodes scapularis Deer tick  Isca\ISCW019551
Orthologs in non-Arthropod Metazoa (EOG6KHFP8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\T04F3.2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028184gn
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10710 Drosophila sechellia Dsec\GM21178 Drosophila erecta Dere\GG24133 Drosophila yakuba Dyak\GE19332 Drosophila ananassae Dana\GF12013 Drosophila pseudoobscura pseudoobscura Dpse\GA15334 Drosophila persimilis Dper\GL11515 Drosophila willistoni Dwil\GK21959 Drosophila virilis Dvir\GJ19867 Drosophila mojavensis Dmoj\GI20098 Drosophila grimshawi Dgri\GH21645
Stocks & Reagents
Stocks Listed in FlyBase ( 12 )
Bloomington	17039 w1118; P{EP}maguEP2277 29670 y1 w*; P{EP}maguG12350 30694 y1 w*; Mi{MIC}maguMI00293/CyO 42049 y1 w*; Mi{MIC}maguMI02087/SM6a 41641 y1 sc* v1; P{TRiP.GL01223}attP2 13939 y1 w67c23; P{SUPor-P}KG02847a P{SUPor-P}maguKG02847b
Harvard	- P{XP}magud00225 - P{XP}magud00269 - PBac{RB}mague00053 - PBac{RB}mague00439 - PBac{WH}maguf02256
VDRC	v106494 P{KK108507}VIE-260B
Genomic Clones ( 2 )
	BACR06J01 BACR09J12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 40 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD30894
Other clones	FI05255
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH08680 GH26795 GM04449 GM08483 RE05064 RE11456 RE17346 RE22194 RE34954 RE66350 RE72452 RE75162
Other clones	11682 30435 73678 101527 106425 153668 191678 EK139655 EK228316 EK232048 EK257464 EK291744 EK294257 ESG013b.E21_C03 FGC019G11 FGC032C06 FGH106B09 FGH124A02 FGM222H10 FGM228C10 FGM231B06 GM05418 LD47165 RP004006496 RP004132830 RP004307856
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0262169 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36048
Antibody Information
	polyclonal (Vuilleumier et al., 2010, Zheng et al., 2011)
Other Information
Discoverer
Etymology
	five and "gone") refers to the missing L5 wing vein phenotype. (Vuilleumier et al., 2010) Named 'magu' after an immortal deity from Chinese mythology. (Li and Tower, 2009) The name "pentagone" (derived from "penta" meaning (Vuilleumier et al., 2010)
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: magu CG2264 (Li and Tower, 2009)
Source for database merge of	Source for merge of: magu pent (Vuilleumier et al., 2010)
Additional comments
Other Comments
	magu mutant testes appear thinner, containing fewer germ cells. (Zheng et al., 2011) Overexpression of magu preferentially in adult motor-neurons increases female life span. (Li and Tower, 2009) Ubiquitous overexpression of magu in adult females modulates fecundity. (Li and Tower, 2009)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136704 NM_165730 NM_165732 NM_001103784 RefSeq (Proteins) • NP_610548 NP_724871 NP_724873 NP_001097254 DDBJ / EMBL / GenBank • AA950777 AAF58854 AAG22291 AAL13844 AAM71052 AAM71053 AAM71054 AAO85264 AQ073453 AW943055 AY058615 AY242076 BH809421 CZ471467 CZ471468 CZ472552 CZ475770 CZ477963 CZ478326 CZ480027 CZ484765 Entrez Gene - A searchable database of RefSeq genes. • 36048 UniProtKB/TrEMBL • A4UZB1 Q0E9E0 Q86MK1 Q95TQ2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Thyroglobulin type-1 (IPR000716) EF-hand domain (IPR002048) Kazal domain (IPR002350) EF-hand-like domain (IPR011992) EF-Hand 1, calcium-binding site (IPR018247) SPARC/Testican, calcium-binding domain (IPR019577)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0262169 DRSC - Results from RNAi screens. • FBgn0262169 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0262169 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36048 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36048 modMine - Data generated by the modENCODE project. • FBgn0262169
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG2264 (Vuilleumier et al., 2010, Firth and Baker, 2007, Li and Tower, 2009, Terry et al., 2006, Keleman et al., 2009.8.5, Zheng et al., 2011, Ni et al., 2010.12.1) CT7504 (Hynes and Zhao, 2000) magu (Wasbrough et al., 2010, Zheng et al., 2011) PENT (Szuperák et al., 2011) pent (Ben-Zvi et al., 2011, Vuilleumier et al., 2010, Vuilleumier et al., 2008, Zheng et al., 2011)
Name Synonym	magu   Pentagone (Ben-Zvi et al., 2011) pentagone (Vuilleumier et al., 2010, Vuilleumier et al., 2008)
Secondary FlyBase IDs
	FBgn0033478 FBgn0259114
References ( 32 )
Generate a list of	All references relating to this gene ( 32 )
List References by type	Research paper  ( 12 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) Curated genome annotation  ( 2 )
Recent research papers ( 6 )
	Peterson and O'Connor, 2013, Development 140(3): 649--659 Activin receptor inhibition by Smad2 regulates Drosophila wing disc patterning through BMP-response elements. [FBrf0220378] Weber et al., 2012, PLoS ONE 7(4): e34745 Genome-Wide Association Analysis of Oxidative Stress Resistance in Drosophila melanogaster. [FBrf0218073] Ben-Zvi et al., 2011, Curr. Biol. 21(16): 1391--1396 Expansion-repression mechanism for scaling the dpp activation gradient in Drosophila wing imaginal discs. [FBrf0214705] Hamaratoglu et al., 2011, PLoS Biol. 9(10): e1001182 Dpp signaling activity requires pentagone to scale with tissue size in the growing Drosophila wing imaginal disc. [FBrf0216502] Szuperák et al., 2011, Development 138(4): 715--724 Feedback regulation of Drosophila BMP signaling by the novel extracellular protein Larval Translucida. [FBrf0212881] Zheng et al., 2011, Dev. Biol. 357(1): 202--210 magu is required for germline stem cell self-renewal through BMP signaling in the Drosophila testis. [FBrf0214645] Show all research papers ...