FlyBase Allele Report: Dmel\spn-A[093A]

General Information

Symbol

Dmel\spn-A^093A

Species

D. melanogaster

Name

FlyBase ID

FBal0151428

Feature type

allele

Associated gene

Dmel\spn-A

Associated Insertion(s)

Carried in Construct

Also Known As

spn-A⁰⁹³, spnA⁰⁹³, spnA^093A

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Staeva-Vieira et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q70term.

(Staeva-Vieira et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:30,055,669..30,055,669 [-]

Nucleotide change:

C30055669T

Amino acid change:

Q70term | spn-A-PA; Q13term | spn-A-PB

Reported amino acid change:

Q70term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Staeva-Vieira et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Bloom syndrome

modeled by Blm^D2, Blm^N1

(Lafave et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle | female (with Df(3R)X3F)

(Staeva-Vieira et al., 2003)

radiation sensitive

(Staeva-Vieira et al., 2003)

radiation sensitive (with Df(3R)X3F)

(Staeva-Vieira et al., 2003)

radiation sensitive (with spn-A³)

(McVey et al., 2004)

radiation sensitive (with spn-A^048B)

(Staeva-Vieira et al., 2003)

viable (with Df(3R)X3F)

(Staeva-Vieira et al., 2003)

Phenotype Manifest In

dorsal appendage (with Df(3R)X3F)

(Staeva-Vieira et al., 2003)

dorsal appendage (with spn-A¹)

(Bayer et al., 2020)

dorsal appendage | maternal effect (with spn-A¹)

(Ferguson et al., 2012)

egg chorion (with spn-A¹)

(Barbosa et al., 2007)

egg chorion | dorsal (with Df(3R)X3F)

(Staeva-Vieira et al., 2003)

karyosome (with Df(3R)X3F)

(Staeva-Vieira et al., 2003)

karyosome | germline clone

(Staeva-Vieira et al., 2003)

synaptonemal complex (with Df(3R)X3F)

(Staeva-Vieira et al., 2003)

Detailed Description

Statement

Reference

spn-A^093A/spn-A¹ but not spn-A^093A/+ females lay eggs with defective dorsal appendages (fused, narrowly spaced or missing) when compared to controls.

(Bayer et al., 2020)

Eggs laid by spn-A^093A/spn-A¹ females display a range of ventralisation phenotypes, ranging from fusion at the base of the dorsal appendages through to complete lack of the appendages.

(Ferguson et al., 2012)

spn-A¹/spn-A^093A females produce eggs with ventralised eggshells.

(Barbosa et al., 2007)

spn-A³/spn-A^093A males in which excision of an insertion of P{hsw^a} is induced during development are completely viable.

(LaRocque et al., 2007)

spn-A³/spn-A^093A third instar larvae show increased sensitivity to high doses of gamma irradiation compared to controls.

(McVey et al., 2004)

The complete copia element in w^a.hs has an LTR at each end. Following excision of P{hsw^a}, repair by SDSA (synthesis-dependent strand annealing) can lead to excision of this copia element due to annealing at these LTRs, resulting in red eyed progeny. Incomplete SDSA, where joining has occurred independently of annealing of homologous ends, can delete parts of the w ORF in w^a.hs resulting in yellow eyed progeny. The frequency of red eyed progeny (and therefore of complete SDSA) following excision of P{hsw^a}sd^wa using H{PΔ2-3}HoP2.1 is decreased from 6% to zero in a spn-A³/spn-A^093A background, but the frequency of yellow eyed progeny (and therefore of incomplete SDSA) is increased from 10% to 17%. This background does not significantly affect the proportion of hemizygous lethal sd deletions resulting from this excision, but does decrease the proportion of excisions resulting non-lethal deletions (from 7% to 1.7%).

(McVey et al., 2004)

spn-A^093A/Df(3R)X3F germ-line clones produce oocytes who DNA is less organised than the wild-type karyosome. The DNA clusters along the periphery of the nucleus adjacent to the nuclear membrane. Homozygous mutant animals and spn-A^048B/spn-A^093A mutants are sensitive to X ray irradiation when irradiated at either 24-48 or 48-72 hours after egg laying (AEL). Little difference in viability is seen in irradiated at 0-24 hours AEL. The synaptonemal complex in spn-A^093A/Df(3R)X3F ovaries persists longer than in wild-type, suggesting a delay in the resolution of synapsis. Double strand breaks form at the normal time, but their resolution is delayed. When spn-A^093A/Df(3R)X3F eggs are produced ,about 31% of eggs are wild-type in appearance, 69% have a mild ventralisation phenotype, the dorsal appendages are fused. spn-A^048B/spn-A^093A animals are sensitive to X ray irradiation when irradiated at either 24-48 or 48-72 hours after egg laying (AEL). Little difference in viability is seen in irradiated at 0-24 hours AEL.

(Staeva-Vieira et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

spn-A^093A/spn-A³ has radiation sensitive phenotype, enhanceable by DNAlig4¹⁶⁹

(McVey et al., 2004)

NOT Enhanced by

Statement

Reference

spn-A^093A has radiation sensitive phenotype, non-enhanceable by spn-B¹

(Staeva-Vieira et al., 2003)

Suppressed by

Statement

Reference

Df(3R)PolQ^Δ, spn-A^093A/spn-A³ has radiation sensitive | third instar larval stage phenotype, suppressible by PolQ^+tBa

(Beagan et al., 2017)

Df(3R)PolQ^Δ, spn-A^093A/spn-A³ has radiation sensitive | third instar larval stage phenotype, suppressible by PolQ^ATPase-dead

(Beagan et al., 2017)

spn-A^093A/spn-A³ has visible phenotype, suppressible by eIF1A^BH167/eIF-1A[+]

(Li et al., 2014)

NOT suppressed by

Statement

Reference

Df(3R)PolQ^Δ, spn-A^093A/spn-A³ has radiation sensitive | third instar larval stage phenotype, non-suppressible by PolQ^pol-dead

(Beagan et al., 2017)

spn-A^093A has radiation sensitive phenotype, non-suppressible by spn-B¹

(Staeva-Vieira et al., 2003)

Enhancer of

Statement

Reference

spn-A^093A/spn-A³ is an enhancer of chemical sensitive phenotype of mus302^D1/mus302^Z1882

(Carvajal-Garcia et al., 2020)

NOT Enhancer of

Statement

Reference

spn-A^093A is a non-enhancer of radiation sensitive phenotype of spn-B¹

(Staeva-Vieira et al., 2003)

Suppressor of

Statement

Reference

spn-A^093A/spn-A^093A is a suppressor of lethal phenotype of Blm^N1/Blm^D2, mus81^Nhe

(Trowbridge et al., 2007)

NOT Suppressor of

Statement

Reference

spn-A^093A is a non-suppressor of radiation sensitive phenotype of spn-B¹

(Staeva-Vieira et al., 2003)

Other

Statement

Reference

Df(3R)PolQ^Δ, spn-A^093A/spn-A³ has radiation sensitive | third instar larval stage phenotype

(Beagan et al., 2017)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal - all die during pharate adult stage phenotype

(Kuo et al., 2014)

Fancm⁰⁶⁹³/Df(3R)ED6058, Gen^Z5997, mus81^Nhe, spn-A^093A/spn-A³ has partially lethal - majority die phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, Gen^Z5997, spn-A^093A/spn-A³ has lethal - all die during pupal stage phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mus81^Nhe, spn-A^093A/spn-A³ has partially lethal - majority live phenotype

(Kuo et al., 2014)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal - all die during pupal stage phenotype

(Kuo et al., 2014)

PolQ^D5/PolQ^D2, spn-A^093A/spn-A³ has partially lethal phenotype

(Chan et al., 2010)

Blm^N1/Blm^D2, mus312^D1/mus312^Z1973, spn-A^093A/spn-A³ has lethal | pupal stage phenotype

(Andersen et al., 2009)

DNAlig4¹⁶⁹, spn-A^093A/spn-A³ has viable phenotype

(McVey et al., 2004)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

spn-A^093A/spn-A¹ has dorsal appendage phenotype, non-enhanceable by Xrcc2^CC/Xrcc2^CC

(Bayer et al., 2020)

Suppressed by

Statement

Reference

spn-A^093A/spn-A³ has egg chorion phenotype, suppressible by eIF1A^BH167/eIF-1A[+]

(Li et al., 2014)

spn-A^093A/spn-A¹ has dorsal appendage | maternal effect phenotype, suppressible by Lnk^d07478/Lnk^CR642

(Ferguson et al., 2012)

spn-A^093A/spn-A¹ has egg chorion phenotype, suppressible by mei-W68¹/mei-W68¹

(Barbosa et al., 2007)

spn-A^093A/Df(3R)X3F has egg chorion | dorsal phenotype, suppressible by barr^+tMa/vls^+tMa/Df(2L)pr2b/Df(2L)be408

(Staeva-Vieira et al., 2003)

spn-A^093A/Df(3R)X3F has egg chorion | dorsal phenotype, suppressible by mei-W68¹

(Staeva-Vieira et al., 2003)

spn-A^093A/Df(3R)X3F has dorsal appendage phenotype, suppressible by barr^+tMa/vls^+tMa/Df(2L)pr2b/Df(2L)be408

(Staeva-Vieira et al., 2003)

spn-A^093A/Df(3R)X3F has dorsal appendage phenotype, suppressible by mei-W68¹

(Staeva-Vieira et al., 2003)

spn-A^093A/Df(3R)X3F has karyosome phenotype, suppressible by barr^+tMa/vls^+tMa/Df(2L)pr2b/Df(2L)be408

(Staeva-Vieira et al., 2003)

NOT suppressed by

Statement

Reference

spn-A^093A/spn-A¹ has dorsal appendage phenotype, non-suppressible by Xrcc2^CC/Xrcc2^CC

(Bayer et al., 2020)

Other

Statement

Reference

Xrcc2^CC, spn-A^093A has dorsal appendage phenotype

(Bayer et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xrcc2^CC homozygous females in the heterozygous background of spn-A^093A lay eggs with defective dorsal appendages (fused, narrowly spaced, branched or shortened) when compared to controls.

(Bayer et al., 2020)

The hypersensitivity of Df(3R)mus308^Δ/Df(3R)mus308^Δ;spn-A³/spn-A^093A double mutant third instar larvae to ionizing radiation is rescued by combination with either the mus308^+tBa or the mus308^ATPase-dead transgene but not by mus308^pol-dead.

Df(3R)mus308^Δ/Df(3R)mus308^Δ;spn-A³/spn-A^093A mutants show a significant reduction in the frequency of end joining repair events in a P{hsw[a]}-excision repair assay, which can be rescued with mus308^+tBa and mus308^ATPase-dead but not mus308^pol-dead.

(Beagan et al., 2017)

eIF-1A^BH167 dominantly suppresses the eggshell patterning defects in spn-A³/spn-A^093A mutants.

(Li et al., 2014)

The ventralisation phenotype seen in eggs laid by spn-A^093A/spn-A¹ females is suppressed if the females are also mutant for Lnk^CR642/Lnk^d07478.

(Ferguson et al., 2012)

There is a decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hsw^a} in spn-A^093A mus308^D5/spn-A³ mus308^ZIII-2003 double mutant males compared to the percentage seen in spn-A single mutants. The double mutants show a substantial increase in the percentage of deletion-associated repair events isolated compared to controls, as occurs in mus308 single mutants. These males show increased sterility.

There is a decrease in the percentage of end-joining repair products in studies of excision and repair events of P{hsw^a} in spn-A^093A mus308^D5/spn-A^093A mus308^ZIII-3294 double mutant males compared to the percentage seen in spn-A single mutants. The double mutants show an increase in the percentage of deletion-associated repair events isolated compared to controls, as occurs in mus308 single mutants. These males show increased sterility.

(Chan et al., 2010)

The spn-A³/spn-A^093A transheterozygous condition does not suppress the lethality of mus312^D1/mus312^Z1973, mus309^D2/mus309^N1 double mutants.

(Andersen et al., 2009)

The ventralised eggshell phenotype produced by spn-A¹/spn-A^093A females is suppressed by mei-W68¹.

(Barbosa et al., 2007)

The lethality seen in mei-41^29D males in which excision of an insertion of P{hsw^a} is induced during development is completely suppressed by spn-A³/spn-A^093A.

(LaRocque et al., 2007)

At intermediate doses (400-500 rad) of gamma irradiation, Lig4¹⁶⁹ spn-A³/spn-A^093A double mutant larvae show increased sensitivity to irradiation compared to spn-A³/spn-A^093A single mutants.

(McVey et al., 2004)

spn-A³/spn-A^093A suppresses the increase deletions resulting from the H{PΔ2-3}HoP2.1 driven of excision of P{hsw^a}sd^wa due to a mus309^D2/mus309^D3 background.

(McVey et al., 2004)

The addition of homozygous mei-W68¹ completely suppresses the ventralisation phenotype seen in spn-A^093A/Df(3R)X3F mutants. The addition of homozygous lok (Using a combination of Df(2L)pr2b, Df(2L)be408, vls^+tMa and barr^+tMa) completely suppresses the ventralisation phenotype seen in spn-A^093A/Df(3R)X3F mutants. The karyosome also appears normal in these mutants.

(Staeva-Vieira et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

spn-A⁰⁹³

(Khodaverdian et al., 2024, Thomas et al., 2022, Bayer et al., 2020, Fasulo et al., 2020, Beagan et al., 2017, Alexander et al., 2016, Ferguson et al., 2012, Chan et al., 2010, Andersen et al., 2009, Trowbridge et al., 2007, McVey et al., 2004)

spn-A^093A

(Khodaverdian et al., 2024, Carvajal-Garcia et al., 2020, Do et al., 2014, Kuo et al., 2014, Lafave et al., 2014, Barbosa et al., 2007, LaRocque et al., 2007)

spn-A⁹³

(McVey et al., 2004)

spn-A^93A

(Yoo and McKee, 2005)

spnA⁰⁹³

(Li et al., 2014, Yu and McVey, 2010, Klovstad et al., 2008)

spnA^093A

(Wei et al., 2019, McVey et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (25)

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