FlyBase Allele Report: Scer\GAL4[dsx-GAL4]

General Information

Symbol

Scer\GAL4^dsx-GAL4

Species

S. cerevisiae

Name

FlyBase ID

FBal0244775

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

TI{GAL4}dsx^GAL4

Carried in Construct

Also Known As

dsx-GAL4, dsx^GAL4, dsx^GAL4(1)

Allele class

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

Mutagen

gene targeting by homologous recombination

(Robinett et al., 2010)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}dsx^GAL4

Cytology

Description

Sequence encoding a GAL4 driver has been inserted immediately after the translational start codon of dsx in exon 2, which is common to male and female dsx mRNAs. This GAL4 coding sequence is terminated by a single stop codon. The two codons 3' of the GAL4 stop codon encode different amino acids than those normally found in dsx proteins, but otherwise the dsx sequences both 5' and 3' of the GAL4 insertion site are unaltered in the targeted chromosome. This allele is anticipated, barring internal re-initiation of translation, to be a null dsx allele.

(Robinett et al., 2010)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 17

gonad

(Robinett et al., 2010)

first instar larval stage

genital disc

(Robinett et al., 2010)

second instar larval stage

genital disc

(Robinett et al., 2010)

third instar larval stage

genital disc

Comment: somatic cells

(Robinett et al., 2010)

hub cell

(Robinett et al., 2010)

cyst cell of testis

(Robinett et al., 2010)

testis | basal

(Robinett et al., 2010)

adult stage

adult fruitless P1 (male) neuron | subset of adult DM4 lineage clone

(Ren et al., 2016)

adult fruitless pMP-e (female) neuron of adult DM4 lineage clone

(Ren et al., 2016)

adult fruitless pIP-e (male) neuron | subset of adult DL2 lineage clone

(Ren et al., 2016)

adult fruitless pIP-e (female) neuron | subset of adult DL2 lineage clone

(Ren et al., 2016)

adult DM2 lineage neuron | subset of superior medial protocerebrum

(Ren et al., 2016)

muscle cell of mesothoracic pleurosternal muscle 59

(Shirangi et al., 2013)

muscle cell of wing axillary muscle hg1

(Shirangi et al., 2013)

hub cell

(Robinett et al., 2010)

cyst cell of testis

(Robinett et al., 2010)

terminal epithelial cell of testis

(Robinett et al., 2010)

adult fat body

(Robinett et al., 2010)

adult oenocyte

(Robinett et al., 2010)

muscle cell of leg muscle of leg

(Robinett et al., 2010)

tracheole of abdomen | subset

(Robinett et al., 2010)

tracheole of ovary

(Robinett et al., 2010)

adult Malpighian tubule

(Robinett et al., 2010)

adult salivary gland

(Robinett et al., 2010)

proboscis

(Robinett et al., 2010)

tergosternal muscle cell

(Robinett et al., 2010)

hypodermal muscle cell of A1-7

(Robinett et al., 2010)

adult midgut muscle cell

(Robinett et al., 2010)

muscle cell of mesothoracic segment

(Robinett et al., 2010)

adult proventriculus | subset

(Robinett et al., 2010)

adult anterior midgut | subset

(Robinett et al., 2010)

adult posterior hindgut | subset

(Robinett et al., 2010)

crop | subset

(Robinett et al., 2010)

adult cardial valve

(Robinett et al., 2010)

adult enterocyte

(Robinett et al., 2010)

basal cylinder

(Robinett et al., 2010)

maxillary palp

(Robinett et al., 2010)

antennal segment 2 microchaeta

Comment: base

(Robinett et al., 2010)

third segment of antenna

Comment: nonneuronal

(Robinett et al., 2010)

proboscis muscle cell

(Robinett et al., 2010)

prothoracic leg tarsal taste bristle | subset

(Robinett et al., 2010)

prothoracic metatarsus

(Robinett et al., 2010)

prothoracic tarsal segment 2

(Robinett et al., 2010)

prothoracic tarsal segment 3

(Robinett et al., 2010)

prothoracic tarsal segment 4

(Robinett et al., 2010)

prothoracic tarsal segment 5

(Robinett et al., 2010)

gustatory sensory organ | subset

(Robinett et al., 2010)

cercus

Comment: pericuticular

(Robinett et al., 2010)

male genitalia

Comment: pericuticular

(Robinett et al., 2010)

adult abdominal neuromere

(Robinett et al., 2010)

adult lateral horn

(Robinett et al., 2010)

adult doublesex pLN (male) neuron

(Robinett et al., 2010)

adult doublesex msA TN2 (male) neuron

(Robinett et al., 2010)

adult doublesex msB TN2 (male) neuron

(Robinett et al., 2010)

adult doublesex mtA TN2 (male) neuron

(Robinett et al., 2010)

adult doublesex prA TN2 (male) neuron

(Robinett et al., 2010)

adult doublesex prBl TN2 (male) neuron

(Robinett et al., 2010)

adult doublesex prBm TN2 (male) neuron

(Robinett et al., 2010)

adult doublesex prC TN2 (male) neuron

(Robinett et al., 2010)

prothoracic leg tarsal taste bristle

(Robinett et al., 2010)

adult doublesex pC1 (female) neuron

(Wang et al., 2020)

adult doublesex neuron

(Wang et al., 2020, Wang et al., 2020)

adult stage | male

adult fruitless P1 (male) neuron | subset of adult DM6 lineage clone

(Ren et al., 2016)

ejaculatory bulb muscle cell

(Robinett et al., 2010)

ejaculatory duct

(Robinett et al., 2010)

seminal vesicle

(Robinett et al., 2010)

muscle cell of male genital muscle I

(Robinett et al., 2010)

muscle cell of male genital muscle II

(Robinett et al., 2010)

adult adipose system

(Robinett et al., 2010)

sex comb

Comment: pericuticular

(Robinett et al., 2010)

surstylar long bristle

(Robinett et al., 2010)

adult doublesex pC1 (male) neuron

(Robinett et al., 2010)

adult doublesex pC2l (male) neuron

(Robinett et al., 2010)

adult doublesex pC2m (male) neuron

(Robinett et al., 2010)

adult doublesex aDN neuron

(Robinett et al., 2010)

adult doublesex SN (male) neuron

(Robinett et al., 2010)

adult doublesex pCd (male) neuron

(Robinett et al., 2010)

adult subesophageal lateral glial cell

(Robinett et al., 2010)

adult doublesex TN1 (male) neuron

(Robinett et al., 2010)

adult doublesex TN2 (male) neuron

(Robinett et al., 2010)

descending neuron of the posterior brain DNp13

(Robinett et al., 2010)

adult doublesex pMN3 (male) neuron

(Robinett et al., 2010)

Additional Information

Statement

Reference

Drives expression in dsx-expressing neurons, in gonads, and in many other tissues throughtout development.

(FlyBase Curators, 2017)

Expression driven by ScerGAL4^dsx-GAL4 was restricted via a transgenic cell class-lineage intersection (CLIn) system to a subset of three clusters of sexually dimorphic neurons - PMP-e, pIP-e and a cluster in the superior medial protocerebrum - of specific Type II neuroblast lineages using KlacKDR^stg14. All but one (59-70) male and all 5 female adult fruitless pMP-e neurons are part of the DM4 lineage, the remaining male pMP-e neuron is part of the DM6 lineage, 27-34 male and 10-16 female pIP-e neurons are part of the DL2 lineage, and 3-5 male and 4 female neurons in the superior medial protocerebrum cluster are part of the DM2 lineage. In total, ~66% (~202/306) male and ~79% (~44/56) female ScerGAL4^dsx-GAL4 neurons intersect with KlacKDR^stg14.

(Ren et al., 2016)

A dsx GAL4 reporter is used to characterize the expression pattern of dsx at all stages of development, but particularly in larvae and adults. An extremely detailed description of the dsx expression pattern is presented with particular attention to whether the pattern is sexually dimorphic in the various tissues. Included is a detailed description of the expression pattern in the adult CNS and in adult peripheral sensory structures.

(Robinett et al., 2010)

Marker for

adult doublesex neuron

(Wang et al., 2020)

Reflects expression of

dsx

(Wang et al., 2020, Wang et al., 2020, Bloomington Drosophila Stock Center, 2017, Ren et al., 2016, Robinett et al., 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease