FlyBase Allele Report: Dmel\HDAC6[KO]

General Information

Symbol

Dmel\HDAC6^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0248834

Feature type

allele

Associated gene

Dmel\HDAC6

Associated Insertion(s)

Carried in Construct

Also Known As

dHDAC6^KO

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Du et al., 2010)

Mutagen

(Du et al., 2010)

(Du et al., 2010)

(Du et al., 2010)

(Du et al., 2010)

ends-in gene targeting

(Du et al., 2010)

Progenitor genotype

Cytology

Description

Mutation in the translation start codon and a frame shift in the HDAC6 open reading frame.

(Du et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

X:15,334,058..15,334,065 [+]

Inserted_sequence:

AGATCT

Comment:

Mutation in ATG codon and frameshift introduced by homologous recombination. AAC[ATG]GT (start codon indicated) replaced with AGATCT.

(Du et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Yan et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Hsap\SNCA^UAS.cFa

(Du et al., 2010)

ameliorates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Xiong et al., 2013)

modeled by Hsap\MAPT^UAS.cWa

(Xiong et al., 2013)

model of obesity

is ameliorated by Hsc70-4^UAS.cEa

(Yan et al., 2017)

is ameliorated by Lsd-2^HMS00629

(Yan et al., 2017)

is ameliorated by Lsd-2^KG00149

(Yan et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

HDAC6^KO males exhibit a reduction in sperm motility and defects in pupal spermatid axoneme.

HDAC6^KO wing disc centrioles are shorter.

(Dobbelaere et al., 2020)

HDAC6^KO mutant larvae show a significantly increased response to gentle touch, as compared to controls.

(Yan et al., 2018)

HDAC6^KO mutant third instar larvae display mild but significant increase in the density of perinuclear microtubule network in muscle cells compared to controls at room temperature and their microtubules (MT) are also more resistant to cold-induced destabilization (the perinuclear MTs are significantly denser in HDAC6^KO mutant muscle cells than in wild-type after cold treatment).

(Mao et al., 2017)

HDAC6^KO mutants do not exhibit macro-morphological changes in synaptic features, with the number of T-bars, synaptic vesicles, or mitochondria per boutonic area, not differing from controls. However, using serial-section election microscopy, HDAC6^KO mutant T-bars exhibit significantly smaller 'top sizes' and fewer tethered synaptic vesicles compared to controls.

At low-frequency stimulation, the excitatory junctional current amplitude in HDAC6^KO mutants is marginally smaller.

(Miskiewicz et al., 2014)

Homozygotes show no obvious morphological abnormalities under normal culture conditions.

Mutants do not show any notable difference to wild-type animals in response to oxidative or heat stress.

Mutant adults do not show defects in climbing ability (assayed up to 20 days after eclosion).

(Du et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

HDAC6^KO has abnormal touch response | larval stage phenotype, non-enhanceable by αTub84B^K40A/αTub84B^K40A

(Yan et al., 2018)

HDAC6^KO has abnormal touch perception phenotype, non-enhanceable by αTub84B^K40A/αTub84B^K40A

(Yan et al., 2018)

Suppressed by

Statement

Reference

HDAC6^KO has short lived | nutrition conditional phenotype, suppressible | RU486 conditional by Scer\GAL4^{Mhc.Switch.PO}/Lsd-2^HMS00629

(Yan et al., 2017)

HDAC6^KO has abnormal locomotor behavior | adult stage | progressive phenotype, suppressible by Lsd-2^KG00149

(Yan et al., 2017)

HDAC6^KO has short lived | nutrition conditional phenotype, suppressible by Lsd-2^KG00149

(Yan et al., 2017)

Enhancer of

Statement

Reference

HDAC6^KO is an enhancer of male semi-sterile phenotype of Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO is an enhancer of decreased fecundity | male phenotype of Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO is an enhancer of abnormal neuroanatomy | adult stage | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF

(Du et al., 2010)

HDAC6^KO is an enhancer of abnormal locomotor behavior | adult stage | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF

(Du et al., 2010)

HDAC6^KO is an enhancer of abnormal neuroanatomy | adult stage | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Du et al., 2010)

NOT Enhancer of

Statement

Reference

HDAC6^KO is a non-enhancer of male semi-sterile phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-enhancer of abnormal touch response | third instar larval stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-enhancer of abnormal locomotor behavior phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-enhancer of decreased fecundity | male phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-enhancer of abnormal touch response | third instar larval stage phenotype of Sirt2^5B-2-35

(Dobbelaere et al., 2020)

Suppressor of

Statement

Reference

HDAC6^KO is a suppressor of abnormal locomotor behavior phenotype of Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO/HDAC6[+] is a suppressor of abnormal locomotor behavior | adult stage phenotype of Hsap\TARDBP^{A315T.UAS.cVa}, Scer\GAL4^elav.PU

(Miskiewicz et al., 2014)

NOT Suppressor of

Statement

Reference

HDAC6^KO is a non-suppressor of abnormal touch response | third instar larval stage phenotype of Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO is a non-suppressor of male semi-sterile phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-suppressor of abnormal touch response | third instar larval stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-suppressor of abnormal locomotor behavior phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-suppressor of decreased fecundity | male phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

Other

Statement

Reference

HDAC6^KO, Sirt2^5B-2-35 has viable phenotype

(Dobbelaere et al., 2020)

Cep97^Δ, HDAC6^KO has viable phenotype

(Dobbelaere et al., 2020)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

HDAC6^KO has wing disc | third instar larval stage phenotype, non-enhanceable by Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO has centriole phenotype, non-enhanceable by Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO has spermatozoon phenotype, non-enhanceable by Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO has spermatid axoneme | P-stage phenotype, non-enhanceable by Cep97^Δ

(Dobbelaere et al., 2020)

Suppressed by

Statement

Reference

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, suppressible by Scer\GAL4^Mhc.PK/Hsc70-4^UAS.cEa

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, suppressible by Scer\GAL4^Mhc.PK/Hsc70-4^UAS.cEa

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, suppressible by Scer\GAL4^Mhc.PK/Lsd-2^HMS00629

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, suppressible by Scer\GAL4^Mhc.PK/Lsd-2^HMS00629

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, suppressible by Lsd-2^HMS00629

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, suppressible by Lsd-2^KG00149

(Yan et al., 2017)

NOT suppressed by

Statement

Reference

HDAC6^KO has spermatozoon phenotype, non-suppressible by Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO has spermatid axoneme | P-stage phenotype, non-suppressible by Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO has wing disc | third instar larval stage phenotype, non-suppressible by Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO has centriole phenotype, non-suppressible by Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by Hsc70-4^K71S.UAS/Scer\GAL4^Mhc.PK

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by Hsc70-4^K71S.UAS/Scer\GAL4^Mhc.PK

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/Hsc70-4^D206S.UAS

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/Hsc70-4^D206S.UAS

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/DnaJ-1^UAS.cKa

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/DnaJ-1^UAS.cKa

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by mTor^GL00156/Scer\GAL4^Mhc.PK

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by mTor^GL00156/Scer\GAL4^Mhc.PK

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/Atg8a^{UASp.EGFP,mCherry}

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/Atg8a^{UASp.EGFP,mCherry}

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/Hsl^UAS.EGFP

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/Hsl^UAS.EGFP

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/bmm^UAS.cUa

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/bmm^UAS.cUa

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/Lpin^HMJ30181

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^Mhc.PK/Lpin^HMJ30181

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by Lsd-1³⁸

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by Lsd-1³⁸

(Yan et al., 2017)

HDAC6^KO has mesothoracic tergotrochanter muscle cell | progressive phenotype, non-suppressible by Scer\GAL4^NP5440/Lsd-2^HMS00629

(Yan et al., 2017)

HDAC6^KO has lipid droplet | adult stage | progressive phenotype, non-suppressible by Scer\GAL4^NP5440/Lsd-2^HMS00629

(Yan et al., 2017)

Enhancer of

Statement

Reference

HDAC6^KO is an enhancer of dopaminergic neuron | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^ple.PF

(Du et al., 2010)

HDAC6^KO is an enhancer of ommatidium | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Du et al., 2010)

HDAC6^KO is an enhancer of eye photoreceptor cell | conditional phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^GMR.PU

(Du et al., 2010)

NOT Enhancer of

Statement

Reference

HDAC6^KO is a non-enhancer of spermatozoon phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-enhancer of spermatid axoneme | P-stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-enhancer of wing disc | third instar larval stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-enhancer of wing disc | third instar larval stage phenotype of Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO is a non-enhancer of centriole phenotype of Sirt2^5B-2-35

(Dobbelaere et al., 2020)

Suppressor of

Statement

Reference

HDAC6^KO is a suppressor of primary spermatocyte | P-stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO/HDAC6[+] is a suppressor of t-bar phenotype of Hsap\TARDBP^{A315T.UAS.cVa}, Scer\GAL4^elav.PU

(Miskiewicz et al., 2014)

HDAC6^KO is a suppressor of microtubule phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^C57

(Xiong et al., 2013)

Df(1)ED7294/HDAC6^KO is a suppressor of microtubule phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^C57

(Xiong et al., 2013)

HDAC6^KO is a suppressor of microtubule phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^C57

(Xiong et al., 2013)

Df(1)ED7294/HDAC6^KO is a suppressor of microtubule phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^C57

(Xiong et al., 2013)

HDAC6^KO is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^elav.PU

(Xiong et al., 2013)

HDAC6^KO is a suppressor of NMJ bouton | larval stage phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^elav.PU

(Xiong et al., 2013)

HDAC6^KO is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Xiong et al., 2013)

HDAC6^KO is a suppressor of NMJ bouton | larval stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^elav.PU

(Xiong et al., 2013)

NOT Suppressor of

Statement

Reference

HDAC6^KO is a non-suppressor of spermatid axoneme | P-stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-suppressor of wing disc | third instar larval stage phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-suppressor of wing disc | third instar larval stage phenotype of Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO is a non-suppressor of centriole phenotype of Sirt2^5B-2-35

(Dobbelaere et al., 2020)

HDAC6^KO is a non-suppressor of spermatozoon phenotype of Cep97^Δ

(Dobbelaere et al., 2020)

HDAC6^KO is a non-suppressor of microtubule phenotype of Scer\GAL4^C57, spas^UAS.cTa

(Mao et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

The decrease in perinuclear microtubule density which is seen in larval muscle cells expressing spas^Scer\UAS.cTa under the control of Scer\GAL4^C57 is not altered by the presence of HDAC6^KO.

The decrease in perinuclear microtubule density which is seen in larval muscle cells expressing Kat60^Scer\UAS.cMa under the control of Scer\GAL4^C57 is not altered by the presence of HDAC6^KO. The microtubule fibres are significantly shorter in the double mutant cells.

(Mao et al., 2014)

Xenogenetic Interactions

Statement

Reference

A HDAC6^KO heterozygous background suppresses the larger synaptic T-bar size found upon expression of Hsap\TARDBP^{A315T.cMa.Scer\UAS} under the control of Scer\GAL4^elav.PU.

A HDAC6^KO heterozygous background suppresses the larger synaptic T-bar size found upon expression of Hsap\TARDBP^{A382T.cMa.Scer\UAS} under the control of Scer\GAL4^elav.PU.

While heterozygosity for HDAC6^KO alone has no effect on activity or negative geotaxis, these conditions both significant suppress the activity and geotaxis defects in flies expressing Hsap\TARDBP^{A315T.cMa.Scer\UAS} under the control of Scer\GAL4^elav.PU.

(Miskiewicz et al., 2014)

HDAC6^KO and HDAC6^KO/Df(1)ED7294 each rescue the defects in perinuclear microtubule density seen in the muscles of animals expressing either Hsap\MAPT^Scer\UAS.cWa or Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^C57.

HDAC6^KO completely suppresses the increase in the number of satellite boutons at the neuromuscular junction (NMJ) and the decrease in average NMJ length caused by expression of either Hsap\MAPT^Scer\UAS.cWa or Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^elav.PU.

(Xiong et al., 2013)

HDAC6^KO enhances the loss of dopaminergic (DA) neurons caused by expression of Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF; the neuron degeneration phenotype is apparent at 10 days after eclosion in the double mutant flies, and 20 days after eclosion it is more severe than that seen in the Hsap\SNCA^Scer\UAS.cFa, Scer\GAL4^ple.PF single mutants.

HDAC6^KO enhances the retinal degeneration phenotype seen in adults expressing Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^GMR.PU.

HDAC6^KO enhances the climbing ability defect caused by expression of Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF; defects are seen 10 days after eclosion in the double mutant flies (defects are not seen until 20 days after eclosion in Hsap\SNCA^Scer\UAS.cFa, Scer\GAL4^ple.PF single mutants) and the defects at 20 days after eclosion are more severe in the double mutants.

HDAC6^KO decreases the number of Lewy body-like Hsap\SNCA positive inclusions seen in the brains of adults expressing Hsap\SNCA^Scer\UAS.cFa under the control of Scer\GAL4^ple.PF such that no inclusions are seen in 10 day old flies and the number of inclusions seen in 20 days old flies is reduced.

(Du et al., 2010)

Complementation and Rescue Data

Rescued by

HDAC6^KO is rescued by Scer\GAL4^Mhc.PK/HDAC6^UAS.cDa

(Yan et al., 2017)

HDAC6^KO is rescued by HDAC6^UAS.cDa/Scer\GAL4^C57

(Xiong et al., 2013)

HDAC6^KO is rescued by Scer\GAL4^C57/HDAC6^H237A.UAS

(Xiong et al., 2013)

Partially rescued by

HDAC6^KO is partially rescued by Scer\GAL4^Mhc.PK/HDAC6^N.UAS

(Yan et al., 2017)

HDAC6^KO is partially rescued by HDAC6^H664A.UAS/Scer\GAL4^C57

(Xiong et al., 2013)

Not rescued by

HDAC6^KO is not rescued by HDAC6^UAS.cDa/Scer\GAL4^NP5440

(Yan et al., 2017)

HDAC6^KO is not rescued by Scer\GAL4^Mhc.PK/HDAC6^{H237A.H664A.UAS}

(Yan et al., 2017)

HDAC6^KO is not rescued by HDAC6^{H237A.H664A.UAS}/Scer\GAL4^C57

(Xiong et al., 2013)

Comments

Expression of either HDAC6^Scer\UAS.cDa or HDAC6^{H237A.Scer\UAS} under the control of Scer\GAL4^C57 completely suppresses the ability of HDAC6^KO to rescue the perinuclear microtubule density defects seen in the muscles of animals expressing either Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^C57.

Expression of HDAC6^{H664A.Scer\UAS} under the control of Scer\GAL4^C57 partially suppresses the ability of HDAC6^KO to rescue the perinuclear microtubule density defects seen in the muscles of animals expressing either Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^C57.

Expression of HDAC6^{H237A.H664A.Scer\UAS} under the control of Scer\GAL4^C57 does not affect the ability of HDAC6^KO to rescue the perinuclear microtubule density defects seen in the muscles of animals expressing either Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^C57.

(Xiong et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51182

y¹ w^* HDAC6^KO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

HDAC6(KO)

(Perry et al., 2017)

HDAC6^KO

(Kong et al., 2026, Yan et al., 2018, Mao et al., 2017, Mao et al., 2014, Xiong et al., 2013)

HDAC6^ko

(Miskiewicz et al., 2014)

HDAC6KO

(Vargas et al., 2020)

Hdac6Δ

(Dobbelaere et al., 2020)

dHDAC6^KO

(Yan et al., 2017, Du et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close