FlyBase Gene Report: Dmel\Pepck1

General Information

Symbol

Dmel\Pepck1

Species

D. melanogaster

Name

Phosphoenolpyruvate carboxykinase 1

Annotation Symbol

CG17725

Feature Type

protein_coding_gene

FlyBase ID

FBgn0003067

Gene Model Status

Current

Stock Availability

11 publicly available

Enzyme Name (EC)

phosphoenolpyruvate carboxykinase (GTP) (4.1.1.32)

Gene Summary

Phosphoenolpyruvate carboxykinase 1 (Pepck1) encodes an enzyme that converts oxaloacetate into phosphoenolpyruvate. It catalyzes the first committed step in gluconeogenesis and thus plays an essential role in glucose metabolism. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

Pepck, phosphoenolpyruvate carboxykinase

Key Links

Genomic Location

Cytogenetic map

55D3-55D3

Sequence location

2R:18,536,767..18,539,416 [+]

Recombination map

2-86

RefSeq locus

NT_033778 REGION:18536767..18539416

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables GTP binding

inferred from electronic annotation with InterPro:IPR008209

(InterPro Project Members, 2004-)

enables manganese ion binding

inferred from biological aspect of ancestor with PANTHER:PTN000169226

(GO Reference Genome Project, 2011-)

enables phosphoenolpyruvate carboxykinase (GTP) activity

inferred from biological aspect of ancestor with PANTHER:PTN000169226

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P07379

(Gene Ontology Curators, 2002-)

inferred from electronic annotation with InterPro:IPR018091

(InterPro Project Members, 2004-)

enables phosphoenolpyruvate carboxykinase activity

inferred from electronic annotation with InterPro:IPR008209, InterPro:IPR008210, InterPro:IPR013035, InterPro:IPR035077, InterPro:IPR035078

(InterPro Project Members, 2004-)

enables purine nucleotide binding

inferred from electronic annotation with InterPro:IPR008210, InterPro:IPR013035

(InterPro Project Members, 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in gluconeogenesis

inferred from mutant phenotype

(Bartok et al., 2015)

involved_in glycerol biosynthetic process

inferred from mutant phenotype

(Bartok et al., 2015)

involved_in trehalose biosynthetic process

inferred from mutant phenotype

(Bartok et al., 2015)

involved_in triglyceride biosynthetic process

inferred from mutant phenotype

(Bartok et al., 2015)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in cellular response to glucose stimulus

inferred from biological aspect of ancestor with PANTHER:PTN000169226

(GO Reference Genome Project, 2011-)

involved_in gluconeogenesis

inferred from electronic annotation with InterPro:IPR008209, InterPro:IPR008210, InterPro:IPR013035, InterPro:IPR035077, InterPro:IPR035078

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P07379

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000169226

(GO Reference Genome Project, 2011-)

involved_in glycerol biosynthetic process from pyruvate

inferred from biological aspect of ancestor with PANTHER:PTN000169226

(GO Reference Genome Project, 2011-)

involved_in oxaloacetate metabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000169226

(GO Reference Genome Project, 2011-)

involved_in response to lipid

inferred from biological aspect of ancestor with PANTHER:PTN000169226

(GO Reference Genome Project, 2011-)

involved_in response to starvation

inferred from biological aspect of ancestor with PANTHER:PTN000169226

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN000169226

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:Q9Z2V4

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

CARBOXY-LYASES

Pathway (FlyBase)

GLUCONEOGENESIS

Protein Family (UniProt)

Belongs to the phosphoenolpyruvate carboxykinase (P20007)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

phosphoenolpyruvate carboxykinase (GTP) activity

oxaloacetate + GTP = phosphoenolpyruvate + GDP + CO2 (4.1.1.32)

RHEA 10388:

Summaries

Gene Snapshot

Gene Group (FlyBase)

CARBOXY-LYASES -: Carboxy-lyases are involved in the the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). (Adapted from FBrf0238856).

Pathway (FlyBase)

GLUCONEOGENESIS -: Gluconeogenesis converts non-carbohydrate precursors such as pyruvate, lactate, alanine, glutamine and glycerol into glucose 6-phosphate (Glc-6P) and plays a crucial role in maintaining sugar homeostasis when dietary carbohydrates are limited. The pathway starts in the mitochondrion with the production of oxaloacetate which, via a series of enzymatic reactions and transport, is converted to cytosolic oxaloacetate which in turn is decarboxylated and phosphorylated to form phosphoenolpyruvate. The subsequent steps involved in the production of Glc-6P are the reverse reactions of glycolysis, only the penultimate step, the dephosphorylation of fructose 1,6-phosphate, is carried out by a different enzyme Fbp. In D. melanogaster, Glc-6P is mainly converted into the disaccharide trehalose, the main hemolymph sugar. However, in a subset of neurons, Glc-6P is converted in the endoplasmic reticulum into glucose. (Adapted from FBrf0236342 and PMID:33397651.)

Protein Function (UniProtKB)

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.

(UniProt, P20007)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Pepck: Phosphoenolpyruvate-carboxykinase

Structural gene for the enzyme phosphoenolpyruvate-carboxykinase in Drosophila melanogaster.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pepck1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P20007)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.54

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pepck1-RA

FBtr0086701

NM_079060

2486

647

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pepck1-PA

FBpp0085880

71.1

647

6.45

P20007

NP_523784

AAF57676

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Monomer.

(UniProt, P20007)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P20007

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pepck1 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

(Fisher et al., 2012)

hindgut anlage in statu nascendi

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

foregut anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

amnioserosa

(Fisher et al., 2012)

amnioserosa primordium

(Fisher et al., 2012)

anterior endoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

plasmatocyte primordium

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 10 -- 13

salivary gland body primordium

(Maruyama et al., 2011)

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

anterior midgut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

Malpighian tubule primordium

(Fisher et al., 2012)

plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal head epidermis

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic/larval lymph gland

(Fisher et al., 2012)

embryonic/larval plasmatocyte

(Fisher et al., 2012)

ventral imaginal tissue

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage

(Zinke et al., 1999)

organism

(Zinke et al., 1999)

Additional Descriptive Data

Pepck1 is expressed early in embryonic salivary glands and is upregulated later in fkh mutants.

(Maruyama et al., 2011)

Transcript can be detected from 40 to 70 hours after egg laying in larval extracts.

(Zinke et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pepck1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pepck1

View exonic expression by tissue for Dmel\Pepck1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003067

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37131/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003067

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003067

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003067

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17725

Flygut - An atlas of the Drosophila adult midgut

FBgn0003067

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Pepck1

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pepck1

Transgenic constructs containing regulatory region of Pepck1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of P-stage

Pepck1^Δ84

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Pepck1^GD9451

viable, with Scer\GAL4^Mef2.PR

Pepck1^GD9451

viable, with Scer\GAL4^pnr-MD237

Pepck1^GD9451

viable, with Scer\GAL4^tin.CΔ4

viable (with Df(2R)ED3636)

Pepck1^Δ84

Other Phenotypes

Allele

abnormal feeding behavior (with Df(2R)ED3636)

Pepck1^Δ84

abnormal size | adult stage, with Scer\GAL4^Lsp2.3.1

increased cell death | adult stage | female, with Scer\GAL4^Lsp2.3.1

Phenotype manifest in

Allele

germarium, with Scer\GAL4^Lsp2.3.1

leg, with Scer\GAL4^Dll-em212

Pepck1^GD9451

segment of leg, with Scer\GAL4^Dll-em212

Pepck1^GD9451

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (2)

Hsap\PCK2

13 of 14

Yes

Hsap\PCK1

12 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (2)

Rnor\Pck1

12 of 14

Yes

Rnor\Pck2

9 of 14

Yes

Mus musculus (laboratory mouse) (2)

Mmus\Pck2

13 of 14

Yes

Mmus\Pck1

12 of 14

Xenopus tropicalis (Western clawed frog) (4)

Xtro\pck2

7 of 13

Yes

Xtro\pck1

5 of 13

Xtro\amotl1

1 of 13

Yes

Xtro\LOC100495041

1 of 13

Danio rerio (Zebrafish) (2)

Drer\pck1

12 of 14

Yes

Drer\pck2

12 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\pck-1

12 of 14

Yes

Cele\pck-2

12 of 14

Yes

Cele\pck-3

4 of 14

Yes

Anopheles gambiae (African malaria mosquito) (1)

Agam\AgaP_AGAP003350

10 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pepck1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

Pepck2

11 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Pepck1^GD9451

ameliorates cancer

modeled by brat^HMS01121

(Hussain et al., 2017)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PCK2; phosphoenolpyruvate carboxykinase 2, mitochondrial

13 of 14

614095

PHOSPHOENOLPYRUVATE CARBOXYKINASE DEFICIENCY, MITOCHONDRIAL; PCKDM

PCK1; phosphoenolpyruvate carboxykinase 1

12 of 14

614168

PHOSPHOENOLPYRUVATE CARBOXYKINASE DEFICIENCY, CYTOSOLIC; PCKDC

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Pepck1 - stau

anti bait coimmunoprecipitation, partial DNA sequence identification by hybridization

(Laver et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

bigmax

suppressible

Pepck1

(Bartok et al., 2015)

brat

suppressible

Pepck1

(Hussain et al., 2017)

BubR1

enhanceable

Pepck1

(Hussain et al., 2017)

External Data

Subunit Structure (UniProtKB)

Monomer.

(UniProt, P20007 )

Linkouts

BioGRID - A database of protein and genetic interactions.

62806

DroID - A comprehensive database of gene and protein interactions.

FBgn0003067

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

GLUCONEOGENESIS

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

gluconeogenesis III

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Gluconeogenesis

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Gluconeogenesis

Class of Gene

gene_with_dicistronic_mRNA

(FlyBase Genome Annotators, 2013)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-86

Cytogenetic map

55D3-55D3

Sequence location

2R:18,536,767..18,539,416 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

55D3-55D3

Limits computationally determined from genome sequence between P{lacW}l(2)08770^k04808 and P{EP}EP437

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-86

(FlyBase, 2016)

2-90.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

30599

y¹ w^*; Mi{MIC}Pepck1^MI00026

77083

y¹ sc^* v¹ sev²¹; P{TKO.GS00875}attP40

65087

y¹ sc^* v¹ sev²¹; P{TRiP.HMC05988}attP40

66848

y¹ w^*; Mi{Trojan-GAL4.2}Pepck1^{MI00026-TG4.2} CG45087^{MI00026-TG4.2-X}

NIG-Fly

17725R-1

P{NIG.17725R}1

17725R-2

P{NIG.17725R}2

2RG-1191

y² VhaAC39-1^cho-1 v¹; P{U6-Pepck1.NIG.sgRNA}attP40/CyO

VDRC

v20529

w¹¹¹⁸; P{GD9451}v20529

v50253

w¹¹¹⁸; P{GD16827}v50253

v342785

P{hsFLP}1, y1 w1118; P{HD_CFD01831}attP40/CyO-GFP

v342323

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD02096}attP40/CyO-GFP

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (393)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH57538

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0003067

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0003067

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Transcriptionally downregulated after 2h of insulin stimulation in Kc167 cells. Contains fkh response elements (FHREs) in the genomic upstream or intronic sequences.

(Junger et al., 2003)

Comparison of CpG distribution in the coding region of 121 genes from six species supports the mCpG mutational hotspot explanation of CpG suppression in methylated species at position II-III and III-I.

(Schorderet and Gartler, 1992)

Structural gene for the enzyme phosphoenolpyruvate-carboxykinase.

Relationship to Other Genes

Source for database merge of

Additional comments

One or more of the processed transcripts for this gene contain(s) two non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by Pepck and CG45087.

(FlyBase Genome Annotators, 2013)

Nomenclature History

Source for database identify of

Source for identity of: Pepck1 Pepck

(Bartok et al., 2015)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

143299_at

(Kristensen et al., 2005)

CG17725

(Delamotte et al., 2025, National Institute of Genetics Fly Stocks, 2022-, Córdoba et al., 2016, Bartok et al., 2015, Choo et al., 2015, Hosono et al., 2015, Vartiainen et al., 2014, Sun et al., 2010, Fujikawa et al., 2009, Li et al., 2009, Perkins et al., 2009, Hayden and Bosco, 2008, Riedl et al., 2005, Pile et al., 2003)

Dromel_CG17725_FBtr0086701_pepck_mORF

(Hayden and Bosco, 2008)

PEPCK1

(Lam Wong and Verheyen, 2021)

Pepck

(Cheng et al., 2025, Sen and Cox, 2022, Kierdorf et al., 2020, Kwon et al., 2020, Li et al., 2019, Giebultowicz, 2018, Mattila and Hietakangas, 2017, Transgenic RNAi Project members, 2017-, Córdoba et al., 2016, Gene Disruption Project members, 2016-, Mossman et al., 2016, Choo et al., 2015, Cogni et al., 2015, Kwon et al., 2015, Tsuda-Sakurai et al., 2015, Wagner et al., 2015, Horiguchi et al., 2014, Lavington et al., 2014, Ruiz et al., 2014, Vartiainen et al., 2014, FlyBase Genome Annotators, 2013, Walker et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Sun et al., 2012, Cherbas et al., 2011, Maruyama et al., 2011, Gallach et al., 2010, Haussmann and Soller, 2010, Kong et al., 2010, Sun et al., 2010, Bartkuhn et al., 2009, Fujikawa et al., 2009, Li et al., 2009, Shim et al., 2009, Haussmann et al., 2008, Hayden and Bosco, 2008, Liu and Lehmann, 2008, Riedl et al., 2005, Lindsley and Zimm, 1992)

Pepck1

(Huang and Perrimon, 2026, Kubrak et al., 2026, Li et al., 2026, Goyal et al., 2025, Thompson et al., 2025, De Groef et al., 2024, Hersperger et al., 2024, Yun et al., 2024, Brener et al., 2023, Lovegrove et al., 2023, Deshpande et al., 2022, De Groef et al., 2021, Zirin et al., 2019)

dPEPCK

(Okamura et al., 2007)

pepck

(Ratnawati et al., 2025, Khaerani et al., 2024, Li et al., 2023, Loudhaief et al., 2023, Lourido et al., 2021, Strilbytska et al., 2020, Strilbytska et al., 2020, Gupta et al., 2019, Sharma et al., 2017, Kubrak et al., 2016, Kubrak et al., 2016, Palu and Thummel, 2016, Sieber et al., 2016, Wang et al., 2016, Chatterjee et al., 2014, Chang et al., 2013, Hull-Thompson et al., 2009, Beckstead et al., 2007, Poels et al., 2004)

pepck1

(Chang et al., 2024, Yoshinari et al., 2021, Bartok et al., 2015)

Name Synonyms

Phosphoenolpyruvate carboxy kinase

(Newell et al., 2020)

Phosphoenolpyruvate carboxykinase

(Mossman et al., 2016, Tsuda-Sakurai et al., 2015, Vartiainen et al., 2014, Laver et al., 2013, Hayden and Bosco, 2008, Riedl et al., 2005)

Phosphoenolpyruvate carboxykinase 1

(Ko et al., 2023)

Phosphoenolpyruvate-carboxykinase

(Lindsley and Zimm, 1992)

phosphoenol pyruvate carboxykinase

(Owusu-Ansah and Banerjee, 2009)

phosphoenol-pyruvate carboxykinase

(Kim et al., 2023)

phosphoenolpyruvate carboxy kinase

(Harvey et al., 2008)

phosphoenolpyruvate carboxykinase

(Mattila and Hietakangas, 2017, Kubrak et al., 2016, Chatterjee et al., 2014, Vartiainen et al., 2014, Chhabra et al., 2013, Sun et al., 2012, Boyd et al., 2011, Hull-Thompson et al., 2009, Poels et al., 2004)

Secondary FlyBase IDs

Datasets (0)

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External Crossreferences and Linkouts ( 49 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37131

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P20007

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P20007

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P20007

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0003067

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37131/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0003067

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0003067

FlyMine - An integrated database for Drosophila genomics

FBgn0003067

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG17725

MARRVEL_MODEL - MARRVEL (model organism gene)

37131

Linkouts

BioGRID - A database of protein and genetic interactions.

62806

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0003067

DroID - A comprehensive database of gene and protein interactions.

FBgn0003067

DRSC - Results frm RNAi screens

FBgn0003067

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0003067

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0003067

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

gluconeogenesis III

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG17725

Flygut - An atlas of the Drosophila adult midgut

FBgn0003067

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009072

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Gluconeogenesis

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Gluconeogenesis

References (173)

The following external sites may use different assemblies or annotations than FlyBase.

NA

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