FlyBase Gene Report: Dmel\Polr2I

General Information

Symbol

Dmel\Polr2I

Species

D. melanogaster

Name

RNA polymerase II subunit I

Annotation Symbol

CG3284

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004855

Gene Model Status

Current

Stock Availability

9 publicly available

Enzyme Name (EC)

DNA-directed RNA polymerase (2.7.7.6)

Gene Summary

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB9 is part of the upper jaw surrounding the central large cleft and thought to grab the incoming DNA template (By similarity). (UniProt, P36958)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

RNA polymerase II, RpII15, PolII, Pol II, Rpb9

Key Links

Genomic Location

Cytogenetic map

88B3-88B3

Sequence location

3R:14,309,089..14,310,178 [+]

Recombination map

3-55

RefSeq locus

NT_033777 REGION:14309089..14310178

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

contributes_to DNA-directed RNA polymerase activity

inferred from biological aspect of ancestor with PANTHER:PTN000133051

(GO Reference Genome Project, 2011-)

enables DNA-directed RNA polymerase activity

inferred from electronic annotation with InterPro:IPR012164

(InterPro Project Members, 2004-)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR001222

(InterPro Project Members, 2004-)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR001222

(InterPro Project Members, 2004-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in mitotic cell cycle

inferred from high throughput mutant phenotype

(Ducat et al., 2008)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in DNA-templated transcription

inferred from electronic annotation with InterPro:IPR001222, InterPro:IPR001529, InterPro:IPR012164

(InterPro Project Members, 2004-)

involved_in maintenance of transcriptional fidelity during transcription elongation by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000133107

(GO Reference Genome Project, 2011-)

involved_in transcription by RNA polymerase II

inferred from sequence or structural similarity with UniProtKB:P36954

(Aoyagi and Wassarman, 2000)

involved_in transcription initiation at RNA polymerase II promoter

inferred from biological aspect of ancestor with PANTHER:PTN000133107

(GO Reference Genome Project, 2011-)

involved_in transcription-coupled nucleotide-excision repair

inferred from biological aspect of ancestor with PANTHER:PTN000133107

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Gu et al., 2002)

part_of RNA polymerase II, core complex

inferred from direct assay

(Gu et al., 2002, Liu et al., 1993)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of RNA polymerase II, core complex

inferred from biological aspect of ancestor with PANTHER:PTN000133107

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:P36954

(Aoyagi and Wassarman, 2000)

Gene Group (FlyBase)

RNA POLYMERASE II

RNA POLYMERASE CATALYTIC SUBUNITS

Protein Family (UniProt)

Belongs to the archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family. (P36958)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

DNA-directed RNA polymerase activity

RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate (2.7.7.6)

Summaries

Gene Group (FlyBase)

RNA POLYMERASE II -: RNA Polymerase II (RNAP II) synthesizes mRNAs and a variety of non-coding RNAs, including snRNAs, snoRNAs, microRNAs, piRNAs and lncRNAs. It comprises 12-13 subunits (13 in D. melanogaster), ten of which form a structurally conserved catalytic core with additional subunits located on the periphery. Notably, five RNAP II subunits are shared with the other two nuclear RNAPs. For transcription initiation, RNAPII assembles with the general transcription factors at promoter DNA to form the pre-initiation complex. (Adapted from FBrf0247389, FBrf0228779, FBrf0213652, PMID:25693126, PMID:23463798 and PMID:17318225.)
RNA POLYMERASE CATALYTIC SUBUNITS -: DNA-directed RNA polymerases (RNAPs) catalyze the synthesis of RNA from DNA templates. Nuclear DNA is transcribed by three different multi-subunit polymerases, RNAPs I, II and III, which synthesize mRNAs, rRNAs, tRNAs and other non-coding RNAs. Although composed of between 12-17 subunits, all three nuclear RNAPs contain a ten-subunit catalytic core. Mitochondrial RNA is synthesized by a single subunit RNA polymerase. DNA primases are a class of RNA polymerases that catalyse the synthesis of short RNA primers that are extended by DNA polymerases during DNA replication. (Adapted from PMID:22365827 and PMID:19540940.)

Protein Function (UniProtKB)

(UniProt, P36958)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Polr2I for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P36958)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

9MU8
9MU9

Comments on Gene Model

Gene model reviewed during 5.53

Gene model reviewed during 5.41

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Polr2I-RA

FBtr0082925

NM_169575

701

129

Polr2I-RB

FBtr0309995

NM_001260170

694

129

Polr2I-RC

FBtr0346348

NM_001300397

1035

129

Additional Transcript Data and Comments

Reported size (kB)

1.2, 0.85 (northern blot)

(Harrison et al., 1992)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Polr2I-PA

FBpp0082384

15.1

129

5.22

FBpp0301701

15.1

129

5.22

FBpp0312065

15.1

129

5.22

P36958

NP_001287326

AHN57325

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

129 aa isoforms: Polr2I-PA, Polr2I-PB, Polr2I-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

129 (aa); 15.1 (kD)

(Harrison et al., 1992)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.

(UniProt, P36958)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

9MU8
9MU9

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Polr2I using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpII15_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.32

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

(Harrison et al., 1992)

embryonic stage -- adult stage

(Harrison et al., 1992)

larval stage

organism | ubiquitous

(Harrison et al., 1992)

adult stage

organism | ubiquitous

(Harrison et al., 1992)

Additional Descriptive Data

This is a minor Polr2I transcript present at all developmental times tested.

(Harrison et al., 1992)

Polr2I transcripts are expressed in all developmental stages. They are expressed ubiquitously in embryos, larvae and adults.

(Harrison et al., 1992)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Gu et al., 2002)

part_of RNA polymerase II, core complex

inferred from direct assay

(Gu et al., 2002, Liu et al., 1993)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Polr2I in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Polr2I

View exonic expression by tissue for Dmel\Polr2I

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41741/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004855

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004855

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004855

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3284

Flygut - An atlas of the Drosophila adult midgut

FBgn0004855

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Polr2I

Transgenic constructs containing regulatory region of Polr2I

Aberrations (Deficiencies and Duplications) ( 13 )

Inferred from experimentation ( 13 )

Gene not disrupted in

Df(3R)293γ5

(Mortin et al., 1992)

Df(3R)JY19

(Breen and Harte, 1991)

Df(3R)JY28

(Breen and Harte, 1991)

T(Y;3)red^P4

(Breen and Harte, 1991)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

Polr2I^GD4234

lethal | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Polr2I^GD4234

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Polr2I^GD4234

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Polr2I^GD4234

viable, with Scer\GAL4^tin.CΔ4

Polr2I^GD4234

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

Polr2I^GD4234

some die during pupal stage, with Scer\GAL4^pnr-MD237

Polr2I^GD4234

some die during second instar larval stage

Polr2I^Z23

wild-type

Phenotype manifest in

Allele

capitellum

Polr2I^Z23

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

Polr2I^GD4234

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (9)

Hsap\POLR2I

14 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (11)

Rnor\Polr2i

13 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tceanc2

1 of 14

Rnor\Tceanc

1 of 14

Mus musculus (laboratory mouse) (10)

Mmus\Polr2i

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Yes

Mmus\Tceanc2

1 of 14

Mmus\Tceanc

1 of 14

Xenopus tropicalis (Western clawed frog) (5)

Xtro\polr2i

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (7)

Drer\polr2i

14 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\rpb-9

14 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP008393

12 of 12

Yes

Agam\AgaP_AGAP002375

2 of 12

Agam\AgaP_AGAP009035

2 of 12

Arabidopsis thaliana (thale-cress) (10)

Atha\NRPB9A

12 of 13

Yes

Atha\NRPB9B

12 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Yes

Atha\AT4G18720

1 of 13

Atha\AT5G42325

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\RPB9

13 of 13

Yes

Scer\DST1

2 of 13

Scer\RPA12

2 of 13

Scer\RPC11

2 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\rpb9

12 of 12

Yes

Spom\rpa12

2 of 12

Spom\rpc11

2 of 12

Spom\tfs1

2 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Polr2I. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (4)

4 of 13

4 of 13

2 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Polr2I^GD4234

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

POLR2I; RNA polymerase II subunit I

14 of 14

180662

POLR3K; RNA polymerase III subunit K

3 of 14

606007

LEUKODYSTROPHY, HYPOMYELINATING, 21; HLD21

hypomyelinating leukodystrophy 21

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Polr2I - Polr2A

anti bait coimmunoprecipitation, molecular weight estimation by staining

(Dollinger et al., 2023)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

sev|Ras85D

suppressible

Polr2I

(Maixner et al., 1998)

External Data

Subunit Structure (UniProtKB)

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits.

(UniProt, P36958 )

Linkouts

BioGRID - A database of protein and genetic interactions.

66817

DroID - A comprehensive database of gene and protein interactions.

FBgn0004855

MIST (protein-protein) - An integrated Molecular Interaction Database

41741

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-55

Cytogenetic map

88B3-88B3

Sequence location

3R:14,309,089..14,310,178 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

88B3-88B3

Limits computationally determined from genome sequence between P{lacW}trx^j14A6&P{PZ}trx⁰⁰³⁴⁷ and P{PZ}cv-c⁰⁶⁹⁵¹

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-55

(FlyBase, 2016)

3-53.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

34751

Polr2I^Z23 red¹ e¹/TM6B, Tb¹

77387

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06518}attP40

83612

y¹ sc^* v¹ sev²¹; P{SAM.dCas9.GS05273}attP40

FlyORF

F001275

M{UAS-Polr2I.ORF}ZH-86Fb

F000950

M{UAS-Polr2I.ORF.3xHA}ZH-86Fb

3284R-2

3284R-3

v110724

v11219

w¹¹¹⁸; P{GD4234}v11219

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (72)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD23432

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004855

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004855

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles with misaligned chromosomes when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.

(Goshima et al., 2007)

Used in a phylogenetic analysis, the tree in inferred by parsimony method from RpoB sequences, or homologous, multiple alignment.

(Mollet et al., 1998)

Designation as "RpII215" was in error.

(Kim et al., 1996)

Isolation of the coding sequence and immunoblotting experiments demonstrate the RpII15 gene codes for a small subunit of RNA polymerase II.

(Liu et al., 1993)

The technique of paramagnetic particle-mediated selection of terminated run-on transcripts was used to examine RNA polymerase II pausing and 5' cap formation at high resolution on the heat shock genes Hsp70A, Hsp70B, Hsp26 and Hsp27. Results demonstrate that polymerases pause over a narrow region of each heat shock gene, not at a defined point. 5' capping occurs over a region coincidental with that of pausing.

(Rasmussen and Lis, 1993)

Protein complexes of RNA polymerase II localise to major developmental puffs and heat shock puffs.

(Weeks et al., 1993)

RpII15 is an essential gene required maternally for embryogenesis.

(Harrison et al., 1992)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Polr2I RpII15

(Marygold et al., 2020)

Nomenclature comments

Etymology

Named 'Polr2I' after the human ortholog.

(Marygold et al., 2020)

Synonyms and Secondary IDs (25)

Reported As

Symbol Synonym

CG3284

(Marygold et al., 2020, Schertel et al., 2013, Voßfeldt et al., 2012, Keleman et al., 2009.8.5, Perkins et al., 2009, Ducat et al., 2008, Goshima et al., 2007, Aoyagi and Wassarman, 2000)

(Leatherman et al., 2002, Robertson et al., 1999)

Pol II

(Vorobyeva et al., 2011, Hendrix et al., 2008, Kim et al., 2004, Boehm et al., 2003, Armstrong et al., 2002, Kaplan et al., 2000)

PolII

(Yamada et al., 2020, Shao and Zeitlinger, 2017, Saberi et al., 2015, Chen et al., 2008, Piel et al., 2008, Zhang et al., 2004, Andrulis et al., 2000)

Polr2I

(Marygold et al., 2020)

RNA Pol II

(Kok et al., 2015, Pascual-Garcia et al., 2014, Mito et al., 2005, Meehan et al., 2001)

RNA pol II

(Gilfillan et al., 2006, Farkas et al., 2000)

RNA polII

(Leclerc et al., 2000)

RNAP

(Ma, 2005)

RNAP II

(Erokhin et al., 2011, Leatherman and Jongens, 2003, Mollet et al., 1998, Seydoux and Dunn, 1997)

RNApolII

(Martinho et al., 2004, Sauer et al., 1995, Sauer et al., 1995)

RPB9

(Marygold et al., 2020)

RPII15

(Kondo and Perrimon, 2011, Liu, 1994.2.4, Liu et al., 1993)

RpII15

(Stewart et al., 2024, Baxley et al., 2017, Transgenic RNAi Project members, 2017-, Blythe and Wieschaus, 2015, Matzat et al., 2013, Schertel et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Baxley et al., 2011, Neumüller et al., 2011, Ayroles et al., 2009, Stone and Ayroles, 2009, Ducat et al., 2008, Goshima et al., 2007)

RpII215

(Sureka and Mishra, 2021, Kim et al., 1996)

Rpb9

(Versluis et al., 2024, DeBerardine et al., 2023, Dollinger et al., 2023, Buckley et al., 2014, Tycon et al., 2014)

dRPB9

(Gu et al., 2002, Park et al., 2001)

l(3)88Be

l(3)Z23

(Breen and Harte, 1991)

pol II

(Zhang and Gilmour, 2006)

polII

(Gu et al., 2002, Bentley, 1998, Lis, 1998)

Name Synonyms

RNA pol II

(Khoroshko et al., 2018)

RNA polymerase II 15kD subunit

RNA polymerase II 15kd subunit

(Stone and Ayroles, 2009)

RNA polymerase II subunit I

Secondary FlyBase IDs

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

BDTNP_TFBS

genome binding

ChIP characterization of transcription factor genome binding, Berkeley Drosophila Transcription Factor Network Project.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 66 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41741

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P36958

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P36958

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4K652

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P36958

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41741/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004855

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004855

FlyMine - An integrated database for Drosophila genomics

FBgn0004855

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG3284

MARRVEL_MODEL - MARRVEL (model organism gene)

41741

PDB - An information portal to biological macromolecular structures

9MU8
9MU9

Linkouts

BioGRID - A database of protein and genetic interactions.

66817

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004855

DroID - A comprehensive database of gene and protein interactions.

FBgn0004855

DRSC - Results frm RNAi screens

FBgn0004855

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

RpII15_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004855

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004855

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3284

Flygut - An atlas of the Drosophila adult midgut

FBgn0004855

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0004855

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015369

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

41741

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (132)

The following external sites may use different assemblies or annotations than FlyBase.

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