FlyBase Gene Report: Dmel\Mcm7

General Information

Symbol

Dmel\Mcm7

Species

D. melanogaster

Name

Minichromosome maintenance 7

Annotation Symbol

CG4978

Feature Type

protein_coding_gene

FlyBase ID

FBgn0020633

Gene Model Status

Current

Stock Availability

7 publicly available

Enzyme Name (EC)

DNA helicase (3.6.4.12)

DNA 3'-5' helicase (5.6.2.4)

Gene Summary

Minichromosome maintenance 7 (Mcm7) encodes a component of the MCM2-7 hexamer, which forms part of the CMG complex, together with CDC45L and GINS proteins. CMG complex is the main DNA helicase that functions during DNA replication. [Date last reviewed: 2018-10-04] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

DmMCM7

Key Links

Genomic Location

Cytogenetic map

66E3-66E3

Sequence location

3L:8,891,596..8,894,310 [+]

Recombination map

3-27

RefSeq locus

NT_037436 REGION:8891596..8894310

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

contributes_to 3'-5' DNA helicase activity

inferred from direct assay

(Moyer et al., 2006)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR031327

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008050, InterPro:IPR018525, InterPro:IPR031327

(InterPro Project Members, 2004-)

enables DNA binding

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR031327

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008050, InterPro:IPR018525, InterPro:IPR031327

(InterPro Project Members, 2004-)

enables DNA helicase activity

inferred from electronic annotation with InterPro:IPR008050

(InterPro Project Members, 2004-)

contributes_to single-stranded DNA binding

inferred from sequence or structural similarity with UniProtKB:Q61881

(Gene Ontology Curators, 2002-)

enables single-stranded DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000179711

(GO Reference Genome Project, 2011-)

contributes_to single-stranded DNA helicase activity

inferred from biological aspect of ancestor with PANTHER:PTN000179711

(GO Reference Genome Project, 2011-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in DNA replication initiation

inferred from electronic annotation with InterPro:IPR008050

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000180038

(GO Reference Genome Project, 2011-)

involved_in DNA replication

inferred from electronic annotation with InterPro:IPR018525

(InterPro Project Members, 2004-)

involved_in DNA strand elongation involved in DNA replication

inferred from biological aspect of ancestor with PANTHER:PTN000180038

(GO Reference Genome Project, 2011-)

involved_in double-strand break repair via break-induced replication

inferred from biological aspect of ancestor with PANTHER:PTN000180038

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of CMG complex

inferred from direct assay

(Ilves et al., 2010, Moyer et al., 2006)

part_of MCM complex

inferred from direct assay

(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of MCM complex

inferred from electronic annotation with InterPro:IPR008050

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000179711

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000179712

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

MCM2-7 COMPLEX

CMG COMPLEX

MCM FAMILY HELICASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

3'-5' DNA helicase activity

Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction (5.6.2.4)

DNA helicase activity

ATP + H2O = ADP + phosphate + H(+) (3.6.4.12)

Summaries

Gene Snapshot

Gene Group (FlyBase)

MCM2-7 COMPLEX -: The heterohexameric MCM2-7 complex is the helicase motor of the CMG (Cdc45-MCM-GINS) replicative helicase of eukaryotes. The six MCM subunits are related to one another and are members of the AAA+ (ATPases associated with diverse cellular activities) superfamily. Hydrolysis of ATP by the individual MCM subunits catalyses the conformational changes that drive unwinding of the DNA duplex. However, the eukaryotic MCM complex alone has no or little helicase activity, requiring the other components of the CMG complex (Cdc45 and the GINS complex) for activation. (Adapted from PMID:23412083.)
CMG COMPLEX -: The CMG (Cdc45-MCM-GINS) complex is the eukaryotic replicative helicase, the enzyme that unwinds double-stranded DNA at replication forks. It is a macromolecular assembly of three replication factors: the Cdc45 protein, and the MCM and GINS complexes. All three components are essential for CMG function, with the MCM2-7 complex functioning as the molecular motor that harnesses the energy of ATP hydrolysis to catalyse strand separation. (Adapted from PMID:23412083.)
MCM FAMILY HELICASES -: The MCM (minichromosome maintenance) family comprise DNA helicases sharing a common 'MCM domain'. MCM2-7 form a heterohexameric complex that is essential for initiation of DNA replication and also acts during elongation as the replicative helicase. MCM8 and MCM9 are not part of this complex - they have roles in replication elongation, meiotic recombination and DNA repair. MCM8 and 9 arose early during eukaryotic evolution, though one or both have been lost in multiple lineages: in mammals, MCM8 and MCM9 form a functional heterodimeric complex; D. melanogaster and related species possess MCM8 only; while other species (e.g. yeast, C. elegans) have neither. (Adapted from PMID:23412083, PMID:30743181 and FBrf0188222.)

Protein Function (UniProtKB)

Acts as a component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

(UniProt, Q9XYU0)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mcm7 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9XYU0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mcm7-RA

FBtr0076585

NM_079260

2451

720

Additional Transcript Data and Comments

Reported size (kB)

2.7 (northern blot)

(Ohno et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Mcm7-PA

FBpp0076312

81.3

720

6.99

Q9XYU0

NP_523984

AAF50357

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

720 (aa); 81 (kD predicted)

(Feger, 1999)

717 (aa)

(Ohno et al., 1998)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).

(UniProt, Q9XYU0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcm7 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mcm7_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.33

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

presumptive embryonic/larval central nervous system

(Feger, 1999)

central brain primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

lateral cord neuron

(Fisher et al., 2012)

sensory nervous system primordium

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 9

Comment: reference states 0-4 hr AEL

(Ohno et al., 1998)

adult stage | female

(Ohno et al., 1998)

unfertilized egg stage

unfertilized egg

(Ohno et al., 1998)

Additional Descriptive Data

Mcm7 transcript expression pattern resembles that of DNA replication. Mcm7 transcript is detected at high levels in the central nervous system and at very low levels in the gut of stage 11-12 embryos.

(Feger, 1999)

Mcm7 transcript levels are high in unfertilized eggs, slightly higher in 2-4 hour embryos, and are low after 4 hours of embryogenesis. Levels are low in other developmental stages, with the exception of adult females.

(Ohno et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of CMG complex

inferred from direct assay

(Ilves et al., 2010, Moyer et al., 2006)

part_of MCM complex

inferred from direct assay

(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mcm7 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mcm7

View exonic expression by tissue for Dmel\Mcm7

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020633

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39014/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020633

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020633

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020633

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4978

Flygut - An atlas of the Drosophila adult midgut

FBgn0020633

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Mcm7

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mcm7

Transgenic constructs containing regulatory region of Mcm7

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Mcm7^f03462

Sterility

Allele

fertile

Mcm7^f03462

Other Phenotypes

Allele

radiation sensitive, with Scer\GAL4^ap-md544

Mcm7^RNAi.UAS.cKa

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (9)

Hsap\MCM7

14 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\MCM3

2 of 14

Hsap\MCMDC2

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (9)

Rnor\Mcm7

14 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (8)

Mmus\Mcm7

14 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (12)

Xtro\mcm7

11 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Xtro\zmcm3

2 of 13

Xtro\hdlbp

1 of 13

Xtro\mcm3

1 of 13

Yes

Xtro\satb2

1 of 13

Danio rerio (Zebrafish) (10)

Drer\mcm7

12 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\mcm-7

14 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\AgaP_AGAP005800

12 of 12

Yes

Agam\AgaP_AGAP002581

4 of 12

Yes

Agam\AgaP_AGAP001135

3 of 12

Agam\AgaP_AGAP001901

3 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

Arabidopsis thaliana (thale-cress) (8)

Atha\PRL

13 of 13

Yes

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\MCM7

13 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\mcm7

12 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Escherichia coli (enterobacterium) (1)

Ecol\yifB

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mcm7. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

dpa

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

rec

6 of 13

mei-218

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

MCM9; minichromosome maintenance 9 homologous recombination repair factor

model of ovarian dysgenesis 4

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MCM7; minichromosome maintenance complex component 7

14 of 14

600592

MCM2; minichromosome maintenance complex component 2

3 of 14

116945

DEAFNESS, AUTOSOMAL DOMINANT 70; DFNA70

autosomal dominant nonsyndromic deafness 70

MCM4; minichromosome maintenance complex component 4

3 of 14

602638

IMMUNODEFICIENCY 54; IMD54

immunodeficiency 54

MCM5; minichromosome maintenance complex component 5

3 of 14

602696

MEIER-GORLIN SYNDROME 8; MGORS8

Meier-Gorlin syndrome 8

MCM6; minichromosome maintenance complex component 6

3 of 14

601806

LACTOSE INTOLERANCE, ADULT TYPE

lactose intolerance

MCM8; minichromosome maintenance 8 homologous recombination repair factor

3 of 14

608187

PREMATURE OVARIAN FAILURE 10; POF10

primary ovarian insufficiency 10

MCM9; minichromosome maintenance 9 homologous recombination repair factor

3 of 14

610098

OVARIAN DYSGENESIS 4; ODG4

ovarian dysgenesis 4

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Mcm7 - Cdc45

anti tag coimmunoprecipitation, molecular weight estimation by silver staining, anti tag western blot, cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot, mass spectrometry studies of complexes

(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)

Mcm7 - Dsor1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Mcm7 - Gfat2

experimental knowledge based

(Guruharsha et al., 2011)

Mcm7 - Mcm2

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm7 - Mcm3

cosedimentation, electron microscopy, experimental knowledge based

(Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011)

Mcm7 - Mcm5

cosedimentation, electron microscopy, experimental knowledge based

(Costa et al., 2011, Guruharsha et al., 2011)

Mcm7 - Mcm6

cosedimentation, electron microscopy, experimental knowledge based

(Costa et al., 2011, Guruharsha et al., 2011)

Mcm7 - Ost1

experimental knowledge based

(Guruharsha et al., 2011)

Mcm7 - Psf1

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm7 - Psf2

cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot

(Costa et al., 2011, Moyer et al., 2006)

Mcm7 - Psf3

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm7 - S6k

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Mcm7 - ScsβG

experimental knowledge based

(Guruharsha et al., 2011)

Mcm7 - Sld5

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm7 - dpa

electron microscopy, cosedimentation, experimental knowledge based

(Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).

(UniProt, Q9XYU0 )

Linkouts

BioGRID - A database of protein and genetic interactions.

64419

DroID - A comprehensive database of gene and protein interactions.

FBgn0020633

MIST (protein-protein) - An integrated Molecular Interaction Database

39014

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

DNA replication

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-27

Cytogenetic map

66E3-66E3

Sequence location

3L:8,891,596..8,894,310 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66E3-66E3

Limits computationally determined from genome sequence between P{PZ}l(3)01629⁰¹⁶²⁹&P{PZ}mRpL12¹⁰⁵³⁴ and P{EP}Hsp26^EP3336&P{EP}Hsp26^EP3315

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

71A1-71C2

(Feger, 1999.2.1)

71A1-71C2

(determined by in situ hybridisation)

(Feger, 1999)

66E-66E

(determined by in situ hybridisation)

(Ohno et al., 1998)

Mcm7 maps to 71A1-2/C1-2.

(Feger, 1999)

Experimentally Determined Recombination Data

Location

3-27

(FlyBase, 2016)

3-21.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

18656

w¹¹¹⁸; PBac{WH}Mcm7^f03462

32867

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00654}attP2

FlyORF

F001623

M{UAS-Mcm7.ORF}ZH-86Fb

F000900

M{UAS-Mcm7.ORF.3xHA}ZH-86Fb

NIG-Fly

HMS00654

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00654}attP2

VDRC

v330121

P{VSH330121}attP40

v106648

P{KK108235}VIE-260B

Genomic Clones (25)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (125)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_RE04406

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020633

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020633

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Crevel et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells.

(Moyer et al., 2006)

Relationship to Other Genes

Source for database merge of

Source for merge of: Mcm7 anon- EST:Liang-1.66

(Ashburner, 2004.6.30)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG4978

(Meadows, 2017.1.11, Çiçek et al., 2016, Schertel et al., 2013, Christis and Munro, 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Hughes et al., 2008, Ashburner, 2004.6.30, Brody et al., 2002)

DmMCM7

(Feger, 1999, Ohno, 1998.1.5, Ohno et al., 1998)

DmeMCM7

(Blanton et al., 2005)

MCM7

(Urban et al., 2022, Bhargava et al., 2020, Hua and Orr-Weaver, 2017, Bleichert et al., 2015, Crevel et al., 2011, Kondo and Perrimon, 2011, Ilves et al., 2010, Moshkin et al., 2009, Crevel et al., 2007, Hong et al., 2007, Hediger and Gasser, 2006, Claycomb et al., 2002, Crevel et al., 2001)

McM7

(De Lucia et al., 2005)

Mcp-PCR1

PCR1

(Su et al., 1997)

anon-EST:Liang-1.66

clone 1.66

(Liang and Biggin, 1998)

dMcm7

(Sahashi et al., 2013)

mcm7

(Blumröder et al., 2016, Brumby et al., 2004, Jasper et al., 2002)

Name Synonyms

Minichromosome maintenance 7

(Ohno et al., 1998)

Minichromosome maintenance-PCR1

minichromosome maintenance 7

(Kondo and Perrimon, 2011)

Secondary FlyBase IDs

FBgn0025980

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

modENCODE_replication_factor_ChIP

genome binding

Genome-wide localization of essential replication factors characterized by ChIP-Seq and ChIP-chip.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 67 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39014

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9XYU0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9XYU0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9XYU0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020633

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39014/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0020633

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0020633

FlyMine - An integrated database for Drosophila genomics

FBgn0020633

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4978

MARRVEL_MODEL - MARRVEL (model organism gene)

39014

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

64419

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0020633

DroID - A comprehensive database of gene and protein interactions.

FBgn0020633

DRSC - Results frm RNAi screens

FBgn0020633

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mcm7_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0020633

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0020633

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4978

Flygut - An atlas of the Drosophila adult midgut

FBgn0020633

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0020633

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013713

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

DNA replication

MIST (protein-protein) - An integrated Molecular Interaction Database

39014

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (101)

The following external sites may use different assemblies or annotations than FlyBase.

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