FlyBase Gene Report: Dmel\Hel89B

General Information

Symbol

Dmel\Hel89B

Species

D. melanogaster

Name

Helicase 89B

Annotation Symbol

CG4261

Feature Type

protein_coding_gene

FlyBase ID

FBgn0022787

Gene Model Status

Current

Stock Availability

11 publicly available

Gene Summary

Helicase 89B (Hel89B) encodes a protein that belongs to the SNF2 family of chromatin regulators. Hel89B product binds to large number of sites on polytene chromosomes and positively regulates the expression of antimicrobial peptide genes. [Date last reviewed: 2018-10-04] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

BTAF1, Mot1, 89B helicase

Key Links

Genomic Location

Cytogenetic map

89A11-89A11

Sequence location

3R:15,973,298..15,983,451 [+]

Recombination map

3-58

RefSeq locus

NT_033777 REGION:15973298..15983451

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000330, InterPro:IPR044078

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR044972

(InterPro Project Members, 2004-)

enables ATP-dependent chromatin remodeler activity

non-traceable author statement

(Schneiderman et al., 2009)

enables DNA binding

inferred from electronic annotation with InterPro:IPR044972

(InterPro Project Members, 2004-)

enables TBP-class protein binding

inferred from electronic annotation with InterPro:IPR044972

(InterPro Project Members, 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in negative regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Hsu et al., 2008)

involved_in positive regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Hsu et al., 2008, Yagi and Ip, 2005)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in regulation of transcription by RNA polymerase II

inferred from sequence or structural similarity with SGD:S000006003

(Goldman-Levi et al., 1996)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

SNF2-LIKE CHROMATIN REMODELERS

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Snapshot

Gene Group (FlyBase)

SNF2-LIKE CHROMATIN REMODELERS -: Snf2-like chromatin remodelers are a family of helicase-like proteins that direct energy derived from ATP hydrolysis into the mechanical remodelling of chromatin structure. They are so-called due to the presence of a domain homologous to the helicase-like ATPase domain of the S. cerevisiae Snf2 protein. This domain, the Snf2 domain, consists of two tandem RecA-like folds and contains seven conserved helicase-related sequence motifs that classify it as part of the helicase superfamily 2 (SF2). However, Snf2 proteins are not bona fide helicases, lacking the ability to separate nucleic acid strands. Instead, Snf2 proteins are DNA translocases that apply an ATP-dependent torsional strain to DNA, which provides the necessary force to remodel nucleosomes or in some cases other DNA-protein complexes. (Adapted from PMID:21862382.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Hel89B for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VF02)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.54

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Hel89B-RB

FBtr0301715

NM_169693

6481

1923

Hel89B-RC

FBtr0344471

NM_001300422

6054

1923

Additional Transcript Data and Comments

Reported size (kB)

7.5 (northern blot)

(Crosby et al., 1999)

7.4 (northern blot)

(Goldman-Levi et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Hel89B-PB

FBpp0290929

213.1

1923

7.01

FBpp0310842

213.1

1923

7.01

Q9VF02

NP_001287351

AHN57350

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1923 aa isoforms: Hel89B-PB, Hel89B-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

210 (kD observed)

(Goldman-Levi et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hel89B using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.09

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

yolk nucleus

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval garland cell

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

wandering third instar larval stage

wing pouch | restricted

(Molnar et al., 2012)

wing hinge primordium | restricted

(Molnar et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Goldman-Levi et al., 1996)

Additional Descriptive Data

Hel89B transcripts are observed at all developmental stages.

(Goldman-Levi et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 12

organism | ubiquitous

(Goldman-Levi et al., 1996)

embryonic stage 12 -- 17

larval longitudinal connective

(Goldman-Levi et al., 1996)

embryonic brain

(Goldman-Levi et al., 1996)

larval ventral nerve cord

(Goldman-Levi et al., 1996)

unfertilized egg

(Goldman-Levi et al., 1996)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Hel89B protein is detected in unfertilized eggs and in early embryos where it expressed everywhere but the pole cells. In later stages, the protein becomes highly localized to the ventral nerve cord and brain. Within the ventral nerve cord it is preferentially found in the longitudinal connectives rather than in the commissures.

(Goldman-Levi et al., 1996)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{GawB}Hel89B^NP1326

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval central brain

(Olson et al., 2019)

embryonic/larval dorsal vessel

(Olson et al., 2019)

adult stage

bilateral local interneuron of the adult antennal lobe | subset

(Tanaka et al., 2012)

antennal lobe hub | restricted

(Tanaka et al., 2012)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Hel89B in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Hel89B

View exonic expression by tissue for Dmel\Hel89B

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0022787

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41943/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0022787

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0022787

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0022787

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4261

Flygut - An atlas of the Drosophila adult midgut

FBgn0022787

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Hel89B

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 16 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Hel89B

Transgenic constructs containing regulatory region of Hel89B

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3R)Exel7327

(Ryder, 2004.4.26)

Df(3R)mor⁹

(Crosby et al., 1999)

Df(3R)mor¹⁰

(Crosby et al., 1999)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die

Hel89B⁰⁸⁷²⁴

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Hel89B^GD4237

viable, with Scer\GAL4^Mef2.PR

Hel89B^GD4237

viable, with Scer\GAL4^pnr-MD237

Hel89B^GD4237

viable, with Scer\GAL4^tin.CΔ4

Hel89B^GD4237

Sterility

Allele

male sterile | recessive

Hel89B⁰⁸⁷²⁴

Other Phenotypes

Allele

abnormal adult locomotory behavior | conditional

Hel89B²⁵⁶¹

visible | recessive

Phenotype manifest in

Allele

abdominal sternite 6 | male

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (29)

Hsap\BTAF1

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ERCC6L2

1 of 14

Yes

Hsap\ERCC6L

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (25)

Rnor\Btaf1

14 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Ercc6l

1 of 14

Yes

Rnor\Ercc6

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (25)

Mmus\Btaf1

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Ercc6l

1 of 14

Yes

Mmus\Ercc6

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (22)

Xtro\btaf1

7 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ercc6l

1 of 13

Yes

Xtro\ercc6

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (32)

Drer\btaf1

12 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\ercc6l2

1 of 14

Yes

Drer\ercc6l

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (26)

Cele\btf-1

13 of 14

Yes

Cele\isw-1

2 of 14

Cele\C25F9.4

1 of 14

Yes

Cele\C25F9.5

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\F53H4.6

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\M04C3.2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP001820

12 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (34)

Atha\RGD3

13 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\CHR18

1 of 13

Atha\CHR23

1 of 13

Atha\CHR24

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\SYD

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (16)

Scer\MOT1

13 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (14)

Spom\mot1

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Escherichia coli (enterobacterium) (1)

Ecol\rapA

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Hel89B. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (16)

Etl1

5 of 13

Iswi

5 of 13

brm

4 of 13

4 of 13

3 of 13

3 of 13

3 of 13

kis

3 of 13

lds

3 of 13

Mi-2

3 of 13

okr

3 of 13

XNP

3 of 13

CG7376

2 of 13

dom

2 of 13

Ino80

2 of 13

Marcal1

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

BTAF1; B-TFIID TATA-box binding protein associated factor 1

13 of 14

605191

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Hel89B - Tbp

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

dom

enhanceable

Hel89B

(Ellis et al., 2015)

Ubx

enhanceable

Hel89B

(Crosby et al., 1999)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66997

DroID - A comprehensive database of gene and protein interactions.

FBgn0022787

MIST (protein-protein) - An integrated Molecular Interaction Database

41943

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-58

Cytogenetic map

89A11-89A11

Sequence location

3R:15,973,298..15,983,451 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

89A11-89A11

Limits computationally determined from genome sequence between P{PZ}blp⁰¹⁶¹⁸ and P{PZ}gish⁰⁴⁸⁹⁵&P{EP}EP3171

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

89B-89B

(determined by in situ hybridisation)

(Goldman-Levi et al., 1996, Goldman-Levi et al., 1995)

Experimentally Determined Recombination Data

Location

3-58

(FlyBase, 2016)

3-57.8

(Comeron et al., 2012)

Left of (cM)

(Crosby et al., 1999)

Right of (cM)

Notes

Maps immediately to the left of Sb.

(Crosby et al., 1999)

Stocks and Reagents

Stocks (11)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

43445

w^*; P{GSV1}Hel89B^EP-767.1

21961

w¹¹¹⁸; P{EPg}Hel89B^HP31401

604106

w¹¹¹⁸; P{SX4.lexA::HG}Hel89B^Et38/TM6B, Tb¹

32895

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00684}attP2

81614

w^*; P{CG4287-CG5516-(Rbf2).S}a; Df(3R)16C3a, P{mor⁺11.8}F3a

11732

ry⁵⁰⁶ P{PZ}Hel89B⁰⁸⁷²⁴, l(3)08724⁰⁸⁷²⁴/TM3, ry^RK Sb¹ Ser¹

Kyoto

103989

y^* w^*; P{GawB}Hel89B^NP1326 / TM6, P{UAS-lacZ.UW23-1}UW23-1

NIG-Fly

HMS00684

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00684}attP2

VDRC

v108000

P{KK100662}VIE-260B

v30495

w¹¹¹⁸; P{GD4237}v30495/TM3

v318266

PBac{fTRG00912.sfGFP-TVPTBF}VK00033

Genomic Clones (22)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (27)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP08622

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0022787

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0022787

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Goldman-Levi et al., 1996)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a greater than three-fold increase in AttA activity in response to heat-killed E.coli after ecdysone treatment in S2 cells.

(Kleino et al., 2005)

Cloning and characterisation of Hel89B suggests the protein may be a global transcriptional regulator of many target genes that functions throughout development.

(Goldman-Levi et al., 1996)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

89B

(Adamkewicz et al., 2001)

BTAF1

(Hsu et al., 2008, Tora, 2002, Yu et al., 2002)

CG4261

(Çiçek et al., 2016, Christis and Munro, 2012, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Schneiderman et al., 2009, Molnar et al., 2006)

HEL89B

(Ellis et al., 2015)

Hel89B

(McCarthy et al., 2022, Sarov et al., 2016, Nikalayevich and Ohkura, 2015, Bonnay et al., 2014, Luck et al., 2014, de Celis et al., 2013.9.11, Rettie and Dorus, 2012, Jayanandanan et al., 2011, Wasbrough et al., 2010, Schneiderman et al., 2009, Hsu et al., 2008, Molnar et al., 2006)

Helicase89B

(Yagi and Ip, 2005)

Mot1

(Guglielmi et al., 2013, Hsu et al., 2008)

Name Synonyms

89B Helicase

(Pereira et al., 2001)

89B helicase

(Zak and Crosby, 1997.1.12, Goldman-Levi et al., 1996)

Helicase 89B

(Cammarato et al., 2011)

Helicase89B

(Bonnay et al., 2014, Zsindely et al., 2009, Yagi and Ip, 2005)

Secondary FlyBase IDs

Datasets (0)

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External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41943

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VF02

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VF02

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0022787

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41943/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0022787

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0022787

FlyMine - An integrated database for Drosophila genomics

FBgn0022787

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4261

MARRVEL_MODEL - MARRVEL (model organism gene)

41943

Linkouts

BioGRID - A database of protein and genetic interactions.

66997

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0022787

DroID - A comprehensive database of gene and protein interactions.

FBgn0022787

DRSC - Results frm RNAi screens

FBgn0022787

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0022787

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0022787

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4261

Flygut - An atlas of the Drosophila adult midgut

FBgn0022787

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC005832

MIST (protein-protein) - An integrated Molecular Interaction Database

41943

References (73)

The following external sites may use different assemblies or annotations than FlyBase.

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