FlyBase Gene Report: Dmel\RhoGAP68F

General Information

Symbol

Dmel\RhoGAP68F

Species

D. melanogaster

Name

Rho GTPase activating protein at 68F

Annotation Symbol

CG6811

Feature Type

protein_coding_gene

FlyBase ID

FBgn0036257

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

Rho GTPase activating protein at 68F (RhoGAP68F) encodes a GTPase activating protein acting upon small Rho-family GTPases involved in epithelial morphogenesis. It localizes to Rab4 recycling endosomes, forms a complex with the product of Rab4 and modulates the transport of Rab4 recycling endosomes back to the cell surface. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

CG 6811

Key Links

Genomic Location

Cytogenetic map

68F5-68F6

Sequence location

3L:12,138,868..12,143,380 [+]

Recombination map

3-38

RefSeq locus

NT_037436 REGION:12138868..12143380

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables GTPase activator activity

inferred from direct assay

(Nahm and Lee, 2011, Sanny et al., 2006)

enables small GTPase binding

inferred from physical interaction with FLYBASE:Rab4; FB:FBgn0016701

(de Madrid et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables GTPase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN002910297

(GO Reference Genome Project, 2011-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in imaginal disc-derived leg morphogenesis

inferred from mutant phenotype

(Greenberg and Hatini, 2011)

involved_in negative regulation of endocytic recycling

inferred from direct assay

(de Madrid et al., 2015)

involved_in negative regulation of stress fiber assembly

inferred from direct assay

(Nahm and Lee, 2011)

involved_in positive regulation of synaptic assembly at neuromuscular junction

inferred from direct assay

(Nahm and Lee, 2011)

involved_in Rho protein signal transduction

inferred from direct assay

(Nahm and Lee, 2011, Sanny et al., 2006)

involved_in ventral furrow formation

inferred from mutant phenotype

(Sanny et al., 2006)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in negative regulation of endocytic recycling

inferred from biological aspect of ancestor with PANTHER:PTN001504923

(GO Reference Genome Project, 2011-)

involved_in signal transduction

inferred from electronic annotation with InterPro:IPR000198

(InterPro Project Members, 2004-)

involved_in small GTPase-mediated signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN002910297

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in early endosome

inferred from direct assay

(de Madrid et al., 2015)

located_in recycling endosome

inferred from direct assay

(de Madrid et al., 2015)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002910297

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

RHO GTPASE ACTIVATING PROTEINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

RHO GTPASE ACTIVATING PROTEINS -: RhoGAPs are GTPase activating proteins for Rho family GTPases. (Adapted from PMID:15731001 and FBrf0158741).

Protein Function (UniProtKB)

Functions as a GTPase-activating protein (GAP) for RhoA/Rho1 during gastrulation by converting it to an inactive GDP-bound state.

(UniProt, Q9VTU3)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\RhoGAP68F for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VTU3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.43

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Gene model reviewed during 6.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

RhoGAP68F-RA

FBtr0075997

NM_140295

2196

476

RhoGAP68F-RC

FBtr0334091

NM_001274814

2228

419

RhoGAP68F-RD

FBtr0334092

NM_001274815

2262

476

RhoGAP68F-RE

FBtr0334093

NM_001274816

2193

475

RhoGAP68F-RF

FBtr0481651

NM_001414142

2223

485

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

RhoGAP68F-PA

FBpp0075729

55.0

476

4.71

FBpp0306216

48.7

419

4.99

FBpp0306217

55.0

476

4.71

FBpp0306218

54.8

475

4.70

FBpp0428433

56.2

485

4.79

UYI58706

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

476 aa isoforms: RhoGAP68F-PA, RhoGAP68F-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RhoGAP68F using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.88

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

indirect flight muscle cell

(Katti et al., 2017)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 5

organism | ubiquitous

(Nahm and Lee, 2011)

embryonic stage 13 -- 16

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval dorsal vessel

(Fisher et al., 2012)

germline cell

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 17

larval ventral nerve cord

(Nahm and Lee, 2011)

embryonic brain

(Nahm and Lee, 2011)

prepupal stage P3

leg joint of tarsal segment

(Greenberg and Hatini, 2011)

Additional Descriptive Data

Expression in stage P3 pupal leg discs is restricted to presumptive tarsal joints.

(Greenberg and Hatini, 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in early endosome

inferred from direct assay

(de Madrid et al., 2015)

located_in recycling endosome

inferred from direct assay

(de Madrid et al., 2015)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\RhoGAP68F in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\RhoGAP68F

View exonic expression by tissue for Dmel\RhoGAP68F

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036257

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39385/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036257

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036257

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036257

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6811

Flygut - An atlas of the Drosophila adult midgut

FBgn0036257

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\RhoGAP68F

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of RhoGAP68F

Transgenic constructs containing regulatory region of RhoGAP68F

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel6116

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^αTub84B.PL

RhoGAP68F^RNAi.UAS.cBa

lethal | embryonic stage

RhoGAP68F^EY05896

lethal | embryonic stage | maternal effect

RhoGAP68F^EY05896

partially lethal - majority die

viable

RhoGAP68F^CRISPR

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

RhoGAP68F^GD10653

viable, with Scer\GAL4^pnr-MD237

RhoGAP68F^GD10653

viable, with Scer\GAL4^tin.CΔ4

RhoGAP68F^GD10653

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

RhoGAP68F^EP3152

some die during pupal stage, with Scer\GAL4^Dll-md23

RhoGAP68F^UAS.mCherry

Phenotype manifest in

Allele

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

RhoGAP68F^EP3152

embryonic/first instar larval cuticle

embryonic/first instar larval cuticle | maternal effect

RhoGAP68F^EY05896

endosome, with Scer\GAL4^ptc-559.1

RhoGAP68F^UAS.mCherry

epithelium, with Scer\GAL4^Dll-md23

RhoGAP68F^UAS.mCherry

epithelium, with Scer\GAL4^ptc-559.1

RhoGAP68F^UAS.Tag:FLAG

larval intersegmental nerve, with Scer\GAL4^elav-C155

RhoGAP68F^EP3152

lateral plasma membrane, with Scer\GAL4^Dll-md23

RhoGAP68F^UAS.mCherry

leg, with Scer\GAL4^Dll-md23

RhoGAP68F^UAS.mCherry

ventral furrow

RhoGAP68F^EP3152

wing disc, with Scer\GAL4^ptc-559.1

RhoGAP68F^UAS.Tag:FLAG

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (64)

Hsap\ARHGAP1

13 of 14

Yes

Hsap\ARHGAP8

12 of 14

Yes

Hsap\PRR5-ARHGAP8

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (47)

Rnor\Arhgap1

13 of 14

Yes

Rnor\Arhgap8

12 of 14

Yes

Rnor\Arhgap4

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (48)

Mmus\Arhgap1

12 of 14

Yes

Mmus\Arhgap8

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (39)

Xtro\arhgap1

8 of 13

Yes

Xtro\abr

1 of 13

Xtro\arhgap4

1 of 13

Yes

Xtro\arhgap5

1 of 13

Xtro\arhgap8

1 of 13

Yes

Xtro\arhgap10

1 of 13

Xtro\arhgap11a.1

1 of 13

Yes

Xtro\arhgap11a.2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\syde2

1 of 13

Xtro\tagap

1 of 13

Danio rerio (Zebrafish) (61)

Drer\arhgap1

13 of 14

Yes

Drer\arhgap4a

1 of 14

Drer\arhgap4b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:ch211-247j9.1

1 of 14

Drer\si:dkey-191m6.4

1 of 14

Drer\si:dkey-219e20.2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (18)

Cele\rga-1

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (22)

Agam\AgaP_AGAP007298

6 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (9)

Atha\AT5G61530

4 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\ECM25

3 of 13

Yes

Scer\BEM3

1 of 13

Scer\LRG1

1 of 13

Scer\RGA1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\SPAC1565.02c

9 of 12

Yes

Spom\rga1

1 of 12

Spom\rga2

1 of 12

Spom\rga6

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:RhoGAP68F. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (20)

3 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

tum

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

RhoGAP68F^KK102738

exacerbates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(McGurk and Bonini, 2012)

exacerbates cancer

modeled by Delta^UAS.cDa, lola^GS88A8, psq^GS88A8

(Even et al., 2019)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ARHGAP1; Rho GTPase activating protein 1

13 of 14

602732

ARHGAP8; Rho GTPase activating protein 8

12 of 14

609405

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

RhoGAP68F - CG11380

two hybrid

(Hu et al., 2017.6.13)

RhoGAP68F - Rab4

anti tag coimmunoprecipitation, anti tag western blot

(de Madrid et al., 2015)

RhoGAP68F - Vta1

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

RhoGAP68F

enhanceable

Rab7

(de Madrid et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Delta

enhanceable

RhoGAP68F

(Even et al., 2019)

Fas3

suppressible

RhoGAP68F

(de Madrid et al., 2015)

suppressible

RhoGAP68F

(Singh et al., 2005)

lola|psq|Delta

enhanceable

RhoGAP68F

(Even et al., 2019)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64739

DroID - A comprehensive database of gene and protein interactions.

FBgn0036257

MIST (protein-protein) - An integrated Molecular Interaction Database

39385

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-38

Cytogenetic map

68F5-68F6

Sequence location

3L:12,138,868..12,143,380 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

68F5-68F6

Limits computationally determined from genome sequence between P{PZ}rols⁰⁸²³²&P{lacW}l(3)j2D3^j2D3 and P{PZ}l(3)05088⁰⁵⁰⁸⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-38

(FlyBase, 2016)

3-30.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

31131

y¹ v¹; P{TRiP.JF01605}attP2

41990

y¹ v¹; P{TRiP.HMS02390}attP40

6442

y¹ w^*; P{UAS-RhoGAP68F.RNAi.B}4.1

56066

y¹ w^*; Mi{MIC}RhoGAP68F^MI10749

15813

y¹ w^67c23; P{EPgy2}RhoGAP68F^EY05896

36620

y¹ sc^* v¹ sev²¹; P{TRiP.GL00580}attP2

VDRC

v107775

P{KK102738}VIE-260B

v34249

w¹¹¹⁸; P{GD10653}v34249/CyO

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (156)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0036257

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0036257

Antibody Information

Laboratory Generated Antibodies

polyclonal

(de Madrid et al., 2015)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

RhoGAP68F localises preferentially to Rab4 endosomes and forms a complex with the Rab4 protein. RhoGAP68F inhibits the direct return of Rab4 endosomes containing Fas3 and shg back to the cell surface. The upregulation of RhoGAP68F during prepupal development is thus predicted to result in decreased transport of key cell adhesion proteins to the cell surface, decreasing the strength of cell-cell contacts and facilitating epithelial remodeling during morphogenesis.

(de Madrid et al., 2015)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG 6811

(Kraut et al., 2001, Kraut et al., 2001)

CG6811

(Hosono et al., 2015, Greenberg and Hatini, 2011, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Ni et al., 2008, Sanny et al., 2006, Bernards, 2003, Billuart et al., 2001)

RhoGAP

(Sailem et al., 2014)

Name Synonyms

Rho GTPase activating protein at 68F

RhoGAP68F

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 64 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39385

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VTU3

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VTU3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VTU3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0036257

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39385/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0036257

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0036257

FlyMine - An integrated database for Drosophila genomics

FBgn0036257

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6811

MARRVEL_MODEL - MARRVEL (model organism gene)

39385

Linkouts

BioGRID - A database of protein and genetic interactions.

64739

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0036257

DroID - A comprehensive database of gene and protein interactions.

FBgn0036257

DRSC - Results frm RNAi screens

FBgn0036257

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0036257

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0036257

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6811

Flygut - An atlas of the Drosophila adult midgut

FBgn0036257

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0036257

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

MIST (protein-protein) - An integrated Molecular Interaction Database

39385

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (71)

The following external sites may use different assemblies or annotations than FlyBase.

-0.88

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