FlyBase Gene Report: Dmel\Prosap

General Information

Symbol

Dmel\Prosap

Species

D. melanogaster

Name

Prosap

Annotation Symbol

CG30483

Feature Type

protein_coding_gene

FlyBase ID

FBgn0040752

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Prosap (Prosap) encodes a member of SH3 and multiple ankyrin repeat domain (SHANK) family of genes that encode synaptic scaffolding proteins. It regulates synaptic growth and development, including the structure of the neuromuscular junction and synapses of the calyx, and olfactory acuity. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Interactive Fly

Also Known As

Shank

Key Links

Genomic Location

Cytogenetic map

50D3-50E1

Sequence location

2R:14,060,456..14,140,902 [-]

Recombination map

2-68

RefSeq locus

NT_033778 REGION:14060456..14140902

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:homer; FB:FBgn0025777

(Diagana et al., 2002)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ionotropic glutamate receptor binding

inferred from biological aspect of ancestor with PANTHER:PTN001191878

(GO Reference Genome Project, 2011-)

enables synaptic receptor adaptor activity

inferred from sequence or structural similarity with RGD:69264

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN001191878

(GO Reference Genome Project, 2011-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in negative regulation of hippo signaling

inferred from genetic interaction with FLYBASE:wts; FB:FBgn0011739,FLYBASE:yki; FB:FBgn0034970

(Xu et al., 2021)

involved_in positive regulation of cell population proliferation

inferred from genetic interaction with FLYBASE:yki; FB:FBgn0034970

(Xu et al., 2021)

involved_in regulation of non-canonical Wnt signaling pathway

inferred from mutant phenotype

(Harris et al., 2016)

involved_in subsynaptic reticulum organization

inferred from mutant phenotype

(Harris et al., 2016)

involved_in synapse organization

inferred from direct assay

(Wu et al., 2017)

involved_in synaptic assembly at neuromuscular junction

inferred from mutant phenotype

(Harris et al., 2016)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in postsynaptic density assembly

inferred from sequence or structural similarity with MGI:MGI:1930016

(Gene Ontology Curators, 2002-)

involved_in synapse organization

inferred from biological aspect of ancestor with PANTHER:PTN001191878

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Wu et al., 2017)

located_in neuromuscular junction

inferred from direct assay

(Harris et al., 2016)

located_in postsynaptic density

inferred from direct assay

(Harris et al., 2016)

located_in terminal bouton

inferred from direct assay

(Wu et al., 2017)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in dendritic spine

inferred from biological aspect of ancestor with PANTHER:PTN001191880

(GO Reference Genome Project, 2011-)

is_active_in postsynaptic density

inferred from biological aspect of ancestor with PANTHER:PTN001191880

(GO Reference Genome Project, 2011-)

located_in postsynaptic density

inferred from sequence or structural similarity with RGD:69264

(Gene Ontology Curators, 2002-)

colocalizes_with postsynaptic membrane

inferred from biological aspect of ancestor with PANTHER:PTN001191880

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pathway (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Prosap for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z9K8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Prosap-RA

FBtr0087601

NM_137081

7979

1871

Prosap-RC

FBtr0339763

NM_001299473

7541

1725

Prosap-RD

FBtr0339764

NM_001259386

8827

1806

Prosap-RE

FBtr0339765

NM_001299474

7346

1660

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Prosap-PA

FBpp0086727

199.0

1871

8.58

FBpp0308810

183.7

1725

8.54

FBpp0308811

191.8

1806

8.62

FBpp0308812

176.6

1660

8.59

A0A0B4LF25

NP_001286403

AHN56201

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prosap using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prosap_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.06

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval neuromuscular junction

• postsynaptic membrane

(Harris et al., 2016)

larval somatic muscle cell

(Harris et al., 2016)

neuropil of larval ventral nerve cord

(Wu et al., 2017)

calyx of larval mushroom body

(Wu et al., 2017)

embryonic/larval cell body glial cell

(Wu et al., 2017)

neuron of larval central nervous system

(Wu et al., 2017)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Prosap is expressed in the larval brain and ventral nerve cord. It is particularly enriched in the mushroom body calyx and in the ventral nerve cord neuropil. It is expressed in most neurons of the larval CNS and in a subset of glial cells.

(Wu et al., 2017)

Prosap protein is detected in the postsynaptic membrane of larval neuromuscular junctions. Additional labeling in larval muscles is likely to be non-specific.

(Harris et al., 2016)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in axon

inferred from direct assay

(Wu et al., 2017)

located_in neuromuscular junction

inferred from direct assay

(Harris et al., 2016)

located_in postsynaptic density

inferred from direct assay

(Harris et al., 2016)

located_in terminal bouton

inferred from direct assay

(Wu et al., 2017)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Prosap in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Prosap

View exonic expression by tissue for Dmel\Prosap

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

50225/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040752

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040752

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040752

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30483

Flygut - An atlas of the Drosophila adult midgut

FBgn0040752

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 26 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Prosap

Transgenic constructs containing regulatory region of Prosap

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)8328

(Wu et al., 2017)

Gene partially disrupted in

In(2LR)O

(Miller et al., 2018)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Prosap^GD10101

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Prosap^GD10101

viable, with Scer\GAL4^Mef2.PR

Prosap^GD10101

viable, with Scer\GAL4^tin.CΔ4

Prosap^GD10101

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal body color, with Scer\GAL4^pnr-MD237

Prosap^GD10101

abnormal learning | adult stage

abnormal locomotor behavior | adult stage

abnormal neuroanatomy | adult stage

abnormal neuroanatomy | dominant | larval stage

abnormal neuroanatomy | dominant | third instar larval stage

Prosap^D101

abnormal neuroanatomy | larval stage (with Df(2R)8328)

abnormal neuroanatomy | larval stage (with Prosap^8k)

Prosap^D101

abnormal neuroanatomy | larval stage (with Prosap^D101)

Prosap^8k

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^how-24B

Prosap^UASp.cHa

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^Mef2.PR

Prosap^UASp.cHa

abnormal neuroanatomy | third instar larval stage (with Df(2R)BSC361)

Prosap^D101

abnormal neurophysiology | larval stage

Prosap^8k

abnormal size (with Df(2R)8328)

abnormal size (with Prosap^8k)

Prosap^D101

abnormal size (with Prosap^D101)

Prosap^8k

abnormal size | adult stage

Prosap^8k

abnormal size | dominant | larval stage

some die during pupal stage, with Scer\GAL4^pnr-MD237

Prosap^GD10101

visible, with Scer\GAL4^GMR.PU

Prosap^KK101537

visible, with Scer\GAL4^pnr-MD237

Prosap^GD10101

Phenotype manifest in

Allele

antennal lobe projection neuron

Prosap^D101

antennal lobe projection neuron (with Df(2R)8328)

Prosap^D101

antennal lobe projection neuron | larval stage

Prosap^8k

antennal lobe projection neuron | larval stage (with Df(2R)8328)

Prosap^8k

antennal lobe projection neuron | larval stage (with Prosap^8k)

Prosap^D101

antennal lobe projection neuron | larval stage (with Prosap^D101)

Prosap^8k

calyx of adult mushroom body

chaeta | increased number, with Scer\GAL4^pnr-MD237

Prosap^GD10101

embryonic/larval neuromuscular junction | third instar larval stage

Prosap^D101

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^how-24B

Prosap^UASp.cHa

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^Mef2.PR

Prosap^UASp.cHa

embryonic/larval neuromuscular junction | third instar larval stage (with Df(2R)BSC361)

Prosap^D101

eye, with Scer\GAL4^GMR.PU

Prosap^KK101537

larval mushroom body calyx glomerulus

larval mushroom body calyx glomerulus (with Df(2R)8328)

larval mushroom body calyx glomerulus (with Prosap^8k)

Prosap^D101

larval mushroom body calyx glomerulus (with Prosap^D101)

Prosap^8k

mesonotum, with Scer\GAL4^pnr-MD237

Prosap^GD10101

NMJ bouton | third instar larval stage

Prosap^D101

NMJ bouton | third instar larval stage, with Scer\GAL4^how-24B

Prosap^UASp.cHa

NMJ bouton | third instar larval stage, with Scer\GAL4^Mef2.PR

Prosap^UASp.cHa

NMJ bouton | third instar larval stage (with Df(2R)BSC361)

Prosap^D101

subsynaptic reticulum | third instar larval stage

Prosap^D101

synapse | adult stage

Prosap^8k

T-bar | adult stage

Prosap^8k

terminal bouton

Prosap^D101

terminal bouton (with Df(2R)8328)

Prosap^D101

terminal bouton | adult stage

terminal bouton | larval stage

Prosap^8k

terminal bouton | larval stage (with Df(2R)8328)

Prosap^8k

terminal bouton | larval stage (with Prosap^8k)

Prosap^D101

terminal bouton | larval stage (with Prosap^D101)

Prosap^8k

trichogen cell, with Scer\GAL4^pnr-MD237

Prosap^GD10101

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (27)

Hsap\SHANK1

10 of 14

Yes

Hsap\SHANK3

10 of 14

Yes

Hsap\SHANK2

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (26)

Rnor\Shank1

10 of 14

Yes

Rnor\Shank2

10 of 14

Yes

Rnor\Shank3

10 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (20)

Mmus\Shank2

10 of 14

Yes

Mmus\Shank3

10 of 14

Yes

Mmus\Shank1

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (17)

Xtro\shank3

7 of 13

Yes

Xtro\shank1

6 of 13

Yes

Xtro\shank2

4 of 13

Yes

Xtro\ankrd50

1 of 13

Xtro\arhgap21

1 of 13

Xtro\asb9

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (38)

Drer\shank3b

10 of 14

Yes

Drer\shank2b

9 of 14

Yes

Drer\shank3a

9 of 14

Yes

Drer\shank1

7 of 14

Yes

Drer\shank2a

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:dkey-33c12.10

1 of 14

Drer\si:dkey-84o3.2

1 of 14

Drer\si:dkey-112g5.13

1 of 14

Drer\si:dkey-112g5.16

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\shn-1

9 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP010413

11 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\ste4

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Prosap. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (6)

dlg1

1 of 13

dop

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

autism spectrum disorder, susceptibility to, SHANK-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

SHANK3; SH3 and multiple ankyrin repeat domains 3

model of Phelan-McDermid syndrome

IEA

(FlyBase, 2019-)

model of schizophrenia 15

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Prosap^GD10101

exacerbates Vissers-Bodmer syndrome

modeled by Not1^GD4070

(Vissers et al., 2020)

Prosap^KK101537

exacerbates retinitis pigmentosa

modeled by ninaE^G69D.UAS

(Chow et al., 2016)

exacerbates Vissers-Bodmer syndrome

modeled by Not1^GD4070

(Vissers et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SHANK1; SH3 and multiple ankyrin repeat domains 1

10 of 14

604999

SHANK3; SH3 and multiple ankyrin repeat domains 3

10 of 14

606230

SHANK2; SH3 and multiple ankyrin repeat domains 2

9 of 14

603290

AUTISM, SUSCEPTIBILITY TO, 17; AUTS17

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Prosap - homer

two hybrid

(Diagana et al., 2002)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ninaE

enhanceable

Prosap

(Chow et al., 2016)

Not1

enhanceable

Prosap

(Vissers et al., 2020)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

72597

DroID - A comprehensive database of gene and protein interactions.

FBgn0040752

MIST (protein-protein) - An integrated Molecular Interaction Database

50225

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF HIPPO SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Neurexins and neuroligins

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A1Z9K8

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-68

Cytogenetic map

50D3-50E1

Sequence location

2R:14,060,456..14,140,902 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

50D3-50E1

Limits computationally determined from genome sequence between P{lacW}mam^k05821 and P{lacW}l(2)s3475^s3475&P{lacW}Hsc70-5^k04907

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-68

(FlyBase, 2016)

2-74.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

17047

w¹¹¹⁸; P{EP}Prosap^EP2366

24446

w¹¹¹⁸; Mi{ET1}Prosap^MB03234

27284

y¹ v¹; P{TRiP.JF02596}attP2

40909

y¹ v¹; P{TRiP.HMS02157}attP40

41422

y¹ w^*; Mi{MIC}Prosap^MI05540

44806

y¹ w^*; Mi{MIC}Prosap^MI08672

56642

y¹ w^*; Mi{MIC}Prosap^MI11974

13184

y¹ w^67c23; P{SUPor-P}Prosap^KG01030

59422

y¹ w^67c23; Mi{PT-GFSTF.0}Prosap^{MI08672-GFSTF.0}/SM6a

Kyoto

113496

w^*; P{GawB}Prosap^NP4677 / CyO; TM3, Sb¹ Ser¹

VDRC

v103592

P{KK101537}VIE-260B

v44830

w¹¹¹⁸; P{GD10101}v44830

More stocks available...

Genomic Clones (53)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (42)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0040752

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0040752

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Wu et al., 2017, Harris et al., 2016)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Identification: in a yeast two hybrid screen for interactors with homer EVH1 domain.

(Diagana et al., 2002)

Area matching Drosophila EST AA439230.

(Davies, 2001.3.15)

Relationship to Other Genes

Source for database merge of

Source for merge of: Prosap BcDNA:GH23107

(Kuchinke and Thomas, 2002.3.5, FlyBase, 1996-)

Source for merge of: CG13354 CG8122

(FlyBase Genome Annotators, 2002-2003)

Source for merge of: Prosap anon-WO0118547.149

(FlyBase, 1996-)

Additional comments

Annotations CG13354 and CG8122 merged as CG30483 (which corresponds to Prosap) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Source for merge of Prosap BcDNA:GH23107 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Source for merge of Prosap anon-WO0118547.149 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

BcDNA:GH23107

CG13354

CG30483

(National Institute of Genetics Fly Stocks, 2022-, Harris et al., 2016, Schwartz et al., 2016, Hosono et al., 2015, Cammarato et al., 2011, Ni et al., 2010.12.1, Keleman et al., 2009.8.5)

CG8122

(Steinhauer et al., 2009, Robertson, 2001.5.6)

D-ProSAP

(Kuchinke et al., 2001)

ProSAP

(Perry et al., 2007)

Shank

(Coll-Tané et al., 2019, Wu et al., 2017, Harris et al., 2016, Diagana et al., 2002)

Shank3

(Janner et al., 2024)

anon-WO0118547.149

prosap

(Urban et al., 2017, Chow et al., 2016, Iyer et al., 2016, Loganathan et al., 2016, Androschuk et al., 2015, Golovnin et al., 2015)

shank3

(Vissers et al., 2020)

Name Synonyms

Prosap

(Cammarato et al., 2011)

Shank

(Garcia-Mata and Burridge, 2007)

Secondary FlyBase IDs

FBgn0033895
FBgn0047309
FBgn0050483
FBgn0062213

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 63 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

50225

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z9K8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z9K8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

50225/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0040752

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0040752

FlyMine - An integrated database for Drosophila genomics

FBgn0040752

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG30483

MARRVEL_MODEL - MARRVEL (model organism gene)

50225

Linkouts

BioGRID - A database of protein and genetic interactions.

72597

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0040752

DroID - A comprehensive database of gene and protein interactions.

FBgn0040752

DRSC - Results frm RNAi screens

FBgn0040752

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prosap_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0040752

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0040752

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG30483

Flygut - An atlas of the Drosophila adult midgut

FBgn0040752

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0040752

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC007761

MIST (protein-protein) - An integrated Molecular Interaction Database

50225

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Neurexins and neuroligins

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

A1Z9K8

References (103)

The following external sites may use different assemblies or annotations than FlyBase.

-1.06

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