FlyBase Gene Report: Dmel\unc-45

General Information

Symbol

Dmel\unc-45

Species

D. melanogaster

Name

uncoordinated 45

Annotation Symbol

CG2708

Feature Type

protein_coding_gene

FlyBase ID

FBgn0288846

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

uncoordinated 45 (unc-45) encodes a protein that binds the product of Hsp83 and myosin, and may may serve as a scaffold for their interaction. It is required for normal myosin accumulation and appears to play a role in myosin folding and/or protection from stress. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

Tom34, dunc-45, Unc45, l(3)03692, lincRNA.721

Key Links

Genomic Location

Cytogenetic map

84E9-84E9

Sequence location

3R:8,033,301..8,037,403 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:8033301..8037403

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein folding chaperone

inferred from direct assay

(Melkani et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables Hsp90 protein binding

inferred from biological aspect of ancestor with PANTHER:PTN002339981

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in cellular response to heat

inferred from direct assay

(Melkani et al., 2010)

involved_in defense response to Gram-negative bacterium

inferred from high throughput mutant phenotype

(Cronin et al., 2009)

involved_in myosin filament assembly

inferred from mutant phenotype

(Lee et al., 2011)

involved_in negative regulation of innate immune response

inferred from high throughput mutant phenotype

(Cronin et al., 2009)

involved_in protein folding

inferred from direct assay

(Melkani et al., 2010)

involved_in somatic muscle development

inferred from mutant phenotype

(Lee et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in protein folding

inferred from biological aspect of ancestor with PANTHER:PTN002339981

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002339981

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\unc-45 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VHW4)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

3NOW

Comments on Gene Model

Gene model reviewed during 5.49

Gene model reviewed during 5.43

Gene model reviewed during 6.03

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

unc-45-RA

FBtr0081788

NM_080057

3236

947

unc-45-RB

FBtr0347497

NM_001316496

3987

947

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

unc-45-PA

FBpp0081284

105.0

947

5.51

FBpp0312575

105.0

947

5.51

Q9VHW4

NP_001303425

ALI30539

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

947 aa isoforms: unc-45-PA, unc-45-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

3NOW

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\unc-45 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.30

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 11 -- 12

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

somatic muscle primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4

organism

(Lee et al., 2011)

embryonic stage 16

embryonic/larval hypodermal muscle cell

(Lee et al., 2011)

pharyngeal muscle cell

(Lee et al., 2011)

larval visceral muscle cell

(Lee et al., 2011)

embryonic/larval dorsal vessel

(Lee et al., 2011)

adult stage

hypodermal muscle cell of A1-7

(Lee et al., 2011)

indirect flight muscle cell

(Lee et al., 2011)

adult dorsal vessel

(Lee et al., 2011)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

(Lee et al., 2011)

Additional Descriptive Data

unc-45 protein is detected on western blots at all stages tested. At two hours after egg laying, it localizes to the embryonic blastoderm and co-localizes with non-muscle myosin II. At 14hr AEL, localization is similar to muscle myosin, which is expressed strongly in skeletal muscles such as body-wall muscles and pharyngeal muscle. Weaker staining is observed in gut muscle and in the ectoderm. unc-45 protein was also observed in other skeletal muscles examined including embryonic dorsal tube, adult body-wall muscle, adult dorsal tube, and adult indirect flight muscle.

(Lee et al., 2011)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\unc-45 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\unc-45

View exonic expression by tissue for Dmel\unc-45

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44910/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0288846

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0288846

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2708

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of unc-45

Transgenic constructs containing regulatory region of unc-45

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene disrupted in

Df(3R)BSC222

(Gresens and Cook, 2006.12.5)

Df(3R)BSC465

(Christensen et al., 2008.4.15)

Df(3R)BSC513

(Christensen et al., 2008.4.15)

Df(3R)ED5223

(Roote, 2004.7.20, Ryder and Roote, 2004.11.10)

Df(3R)ED5230

(Ryder and Roote, 2004.11.10)

Df(3R)Exel6263

(Ryder, 2004.4.26)

Df(3R)p712

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Gene not disrupted in

Df(3R)Antp17

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Inferred from location ( 13 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^how-24B

unc-45^RNAi.UAS.cUa

lethal, with Scer\GAL4^Mef2.PR

unc-45^GD9815

lethal, with Scer\GAL4^pnr-MD237

unc-45^GD9815

lethal | pupal stage, with Scer\GAL4^pnr-MD237

unc-45^GD9815

lethal | recessive

unc-45⁰³⁶⁹²

lethal - all die before end of larval stage

unc-45^EY03034

viable

unc-45^EY03034

viable, with Scer\GAL4^tin.CΔ4

unc-45^GD9815

Sterility

Allele

fertile

unc-45^EY03034

Other Phenotypes

Allele

abnormal axis specification | germline clone | oogenesis

abnormal cell polarity | germline clone | oogenesis

short lived, with Scer\GAL4^HCH.Hand

unc-45^RNAi.UAS.cUa

some die during third instar larval stage

unc-45^EY03034

Phenotype manifest in

Allele

adult heart, with Scer\GAL4^HCH.Hand

unc-45^RNAi.UAS.cUa

adult heart, with Scer\GAL4^tin.CΔ4, Scer\GAL80^ts.αTub84B

unc-45^RNAi.UAS.cUa

adult heart chamber 1, with Scer\GAL4^HCH.Hand

unc-45^RNAi.UAS.cUa

adult heart chamber 2, with Scer\GAL4^HCH.Hand

unc-45^RNAi.UAS.cUa

egg chamber | germline clone

unc-45^6C3-11

embryonic/larval heart | P-stage, with Scer\GAL4^HCH.Hand

unc-45^RNAi.UAS.cUa

embryonic/larval heart | third instar larval stage, with Scer\GAL4^HCH.Hand

unc-45^RNAi.UAS.cUa

embryonic somatic muscle cell

unc-45^EY03034-33

microtubule | germline clone

myofibril

unc-45^EY03034-33

myofibril, with Scer\GAL4^HCH.Hand

unc-45^RNAi.UAS.cUa

nucleus | germline clone

oocyte | germline clone

sarcomere, with Scer\GAL4^Mef2.PR

unc-45^GD9815

Z disc, with Scer\GAL4^Mef2.PR

unc-45^GD9815

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (46)

Hsap\UNC45B

14 of 14

Yes

Hsap\UNC45A

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\TXNDC5

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (35)

Rnor\Unc45b

14 of 14

Yes

Rnor\Unc45a

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Ttc4

1 of 14

Rnor\Ttc32

1 of 14

Yes

Mus musculus (laboratory mouse) (36)

Mmus\Unc45b

13 of 14

Yes

Mmus\Unc45a

12 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Ttc4

1 of 14

Mmus\Ttc32

1 of 14

Yes

Mmus\Txndc5

1 of 14

Mmus\Ube4a

1 of 14

Mmus\Zc3h7a

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (27)

Xtro\unc45b

11 of 13

Yes

Xtro\unc45a

6 of 13

Yes

Xtro\aip

1 of 13

Xtro\ankar

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (42)

Drer\unc45b

14 of 14

Yes

Drer\unc45a

12 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\ttc4

1 of 14

Drer\ttc12

1 of 14

Drer\ttc32

1 of 14

Yes

Drer\zc3h7a

1 of 14

Yes

Drer\zc3h7ba

1 of 14

Yes

Drer\zc3h7bb

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (11)

Cele\unc-45

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (28)

Agam\AgaP_AGAP003727

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (53)

Atha\AT1G56090

2 of 13

Yes

Atha\Phox1

2 of 13

Yes

Atha\Phox2

2 of 13

Yes

Atha\Phox3

2 of 13

Yes

Atha\Phox4

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT3G20170

1 of 13

Atha\AT3G54790

1 of 13

Atha\AT3G61390

1 of 13

Yes

Atha\AT5G10200

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (11)

Scer\SHE4

7 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (8)

Spom\rng3

7 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:unc-45. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (27)

Dpit47

2 of 13

HIP

2 of 13

HIP-R

2 of 13

Sgt

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

adp

1 of 13

arm

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

shu

1 of 13

sxc

1 of 13

Tpr2

1 of 13

Ube4B

1 of 13

zda

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

UNC45B; unc-45 myosin chaperone B

model of myofibrillar myopathy 11

IEA

(FlyBase, 2019-)

model of cataract 43

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

unc-45⁰³⁶⁹²

exacerbates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

unc-45^EY03034

ameliorates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

unc-45^+t

ameliorates amyloidosis

modeled by Hsap\HTT^{Q72.ex1p.UAS.EGFP}

(Melkani et al., 2013)

ameliorates cardiomyopathy

modeled by Hsap\HTT^{Q72.ex1p.UAS.EGFP}

(Melkani et al., 2013)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

UNC45B; unc-45 myosin chaperone B

14 of 14

611220

UNC45A; unc-45 myosin chaperone A

13 of 14

611219

OSTEOOTOHEPATOENTERIC SYNDROME; OOHE

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

unc-45 - Mhc

dynamic light scattering

(Melkani et al., 2010)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84E9-84E9

Sequence location

3R:8,033,301..8,037,403 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84E9-84E9

Limits computationally determined from genome sequence between P{EP}EP3060^EP3060 and P{PZ}grn⁰⁵⁹³⁰

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84E10-84E11

(determined by in situ hybridisation)

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.7

(Comeron et al., 2012)

(Martin et al., 2003)

Left of (cM)

Right of (cM)

Notes

Maps to 3R.

(Martin et al., 2003)

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

601014

w¹¹¹⁸; P{VT038206-p65.AD}attP40

73722

w¹¹¹⁸; P{VT038205-GAL4.DBD}attP2

74957

w¹¹¹⁸; P{VT038206-GAL4.DBD}attP2

19664

y¹ w^67c23; P{EPgy2}unc-45^EY03034

85110

w¹¹¹⁸; PBac{RB}unc-45^e00294/TM6B, Tb¹

89307

w¹¹¹⁸; P{R80A11-p65.AD}attP40; P{VT038205-GAL4.DBD}attP2

88696

w¹¹¹⁸; P{VT039518-p65.AD}attP40; P{VT038205-GAL4.DBD}attP2

Korea

12715

P{VT038205-GAL4}attP2

12716

P{VT038206-GAL4}attP2

2708R-1

v108868

v33561

w¹¹¹⁸; P{GD9815}v33561

More stocks available...

Genomic Clones (26)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (16)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD10334

Other clones

FI21225

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Lee et al., 2011)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Embryonic injection of dsRNA made from templates generated with primers directed against this gene results in an entirely normal embryonic muscle pattern. However, these muscles never contract (compared to age-matched controls).

(Estrada et al., 2006)

Identification: in a germline clone screen for mutants that are defective in localisation of an Avic\GFP-stau marker in living oocytes. 3 alleles of pup have been identified in the screen.

(Martin et al., 2003)

Relationship to Other Genes

Source for database merge of

Source for merge of: unc-45 pup

(Doerflinger et al., 2022)

Source for merge of: Tom34 CG2708

(Misra, 2000.8.9)

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: unc-45 Tom34

(Checkland, 2002.3.19)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (19)

Reported As

Symbol Synonym

CG2708

(Hosono et al., 2015, Conrad et al., 2012, Melkani et al., 2011, Keleman et al., 2009.8.5, Estrada et al., 2006)

DmUNC-45

(Lee et al., 2011)

DmUnc-45

(Checkland et al., 2002)

Tom34

(Gistelinck et al., 2012, Ambegaokar and Jackson, 2011, Gonsalves et al., 2011, Colin et al., 2009, Cronin et al., 2009, Christensen et al., 2008.4.15, Christensen et al., 2008.4.15, Gresens and Cook, 2006.12.5)

UNC-45

(Riahi et al., 2021, Trujillo et al., 2014)

UNC45

(Karunendiran et al., 2021)

Unc-45

(Trujillo et al., 2024)

Unc45

(Merigliano et al., 2025, Dialynas et al., 2019, Caridi et al., 2018)

dUNC-45

(Lee et al., 2011)

dUnc-45

(Walls et al., 2018)

dUnc45

(Walls et al., 2018)

dunc-45

(Smith et al., 2025, Lee et al., 2011, Melkani et al., 2010, Yu et al., 2005)

l(3)03692

(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

lincRNA.721

(FlyBase Genome Annotators, 2014, Young et al., 2012)

pup

unc-45

(Ricolo et al., 2025, Doerflinger et al., 2022, Lechuga et al., 2022, Carbone et al., 2016, Luck et al., 2014, Melkani et al., 2013, Melkani et al., 2011)

Name Synonyms

Translocase of outer membrane 34

(Spradling et al., 1999)

poulpe

(Doerflinger et al., 2022, Martin et al., 2003)

uncoordinated 45

Secondary FlyBase IDs

FBgn0010812
FBgn0037545
FBgn0067661

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 32 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44910

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VHW4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VHW4

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

44910/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0288846

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0288846

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2708

MARRVEL_MODEL - MARRVEL (model organism gene)

44910

PDB - An information portal to biological macromolecular structures

3NOW

Linkouts

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0288846

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2708

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC015213

References (76)

The following external sites may use different assemblies or annotations than FlyBase.

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