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Citation
Kohl, J., Ng, J., Cachero, S., Ciabatti, E., Dolan, M.J., Sutcliffe, B., Tozer, A., Ruehle, S., Krueger, D., Frechter, S., Branco, T., Tripodi, M., Jefferis, G.S. (2014). Ultrafast tissue staining with chemical tags.  Proc. Natl. Acad. Sci. U.S.A. 111(36): E3805--E3814.
FlyBase ID
FBrf0226212
Publication Type
Research paper
Abstract

Genetically encoded fluorescent proteins and immunostaining are widely used to detect cellular and subcellular structures in fixed biological samples. However, for thick or whole-mount tissue, each approach suffers from limitations, including limited spectral flexibility and lower signal or slow speed, poor penetration, and high background labeling, respectively. We have overcome these limitations by using transgenically expressed chemical tags for rapid, even, high-signal and low-background labeling of thick biological tissues. We first construct a platform of widely applicable transgenic Drosophila reporter lines, demonstrating that chemical labeling can accelerate staining of whole-mount fly brains by a factor of 100. Using viral vectors to deliver chemical tags into the mouse brain, we then demonstrate that this labeling strategy works well in mice. Thus this tag-based approach drastically improves the speed and specificity of labeling genetically marked cells in intact and/or thick biological samples.

PubMed ID
PubMed Central ID
PMC4246963 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Proc. Natl. Acad. Sci. U.S.A.
    Title
    Proceedings of the National Academy of Sciences of the United States of America
    Publication Year
    1915-
    ISBN/ISSN
    0027-8424
    Data From Reference