FlyBase Allele Report: Dmel\tsh[8]

General Information

Symbol

Dmel\tsh⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0032945

Feature type

allele

Associated gene

Dmel\tsh

Associated Insertion(s)

Carried in Construct

Also Known As

Df(2L)tsh⁸

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Andrew et al., 1994, de Zulueta et al., 1994)

loss of function allele

(Laugier et al., 2005, Core et al., 1997, Alexandre et al., 1996)

Mutagen

Delta2-3 transposase

(Fasano et al., 1991)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Fasano et al., 1991)

Description

Molecular deletion specific to the tsh gene in 40A.

(Kerridge, 1996.11.6)

40kb deletion.

(Andrew et al., 1994)

Precise excision of P{A92} insertion.

(Fasano et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Bhojwani et al., 1997)

viable (with tsh^rAO135)

(Kahsai et al., 2016)

visible

(Manfroid et al., 2004)

visible | dominant

(Bhojwani et al., 1997)

visible | homeotic

(Laugier et al., 2005)

visible | somatic clone

(Singh et al., 2002)

Phenotype Manifest In

denticle row 1

(Laugier et al., 2005)

denticle row 2

(Laugier et al., 2005)

denticle row 3

(Laugier et al., 2005)

denticle row 4

(Laugier et al., 2005)

embryonic/first instar larval cuticle

(Manfroid et al., 2004, Gallet et al., 2000)

embryonic/larval cuticle

(Laugier et al., 2005, Gallet et al., 1998)

embryonic labial segment | ectopic

(Manfroid et al., 2004)

embryonic midgut constriction 1

(Mathies et al., 1994)

embryonic midgut constriction 2

(Mathies et al., 1994)

embryonic prothoracic segment

(Manfroid et al., 2004)

eye | dorsal | somatic clone

(Singh et al., 2002)

eye | somatic clone | ventral

(Singh et al., 2002)

Keilin organ

(Core et al., 1997)

larval abdomen

(Core et al., 1997)

larval thorax

(Core et al., 1997)

maxillary palp

(Bhojwani et al., 1997)

prothoracic segment

(Laugier et al., 2005)

ventral denticle belt

(Saller et al., 2002, Gallet et al., 2000)

ventral denticle belt | larval stage

(Gallet et al., 1998)

Detailed Description

Statement

Reference

In tsh⁸ larvae, the T1 segment adopts a labial identity. Such larvae also show severe disruption of the naked cuticle and in the abdominal segments, denticle types 1-4 are missing.

(Laugier et al., 2005)

Changes in cuticle pattern in tsh⁸/tsh⁸ embryos indicate that T1 identity is transformed to labial identity (characterized by loss of T1 cuticle). In addition, trunk segments exhibit a weak segment polarity phenotype.

(Manfroid et al., 2004)

Mutant embryos have reduced denticle belts containing only tapered denticle of rows 5 and 6.

(Saller et al., 2002)

Homozygous clones in the ventral margin of the eye result in ventral eye enlargements. Internal ventral clones in the eye do not significantly affect eye development. Clones in the dorsal eye suppress eye formation (there is an eye-to-cuticle transformation).

(Singh et al., 2002)

Mutant embryos show a weak segment polarity phenotype with disruption of both naked cuticle and denticle belt organisation. Only denticle types 5 and 6 are made.

(Gallet et al., 2000)

Mutant larvae have abnormal denticle belts, and denticles are present in the regions of naked cuticle, especially in the ventral midline.

(Gallet et al., 1998)

X ray induced clones in the developing eye show normal ommatidial organization.

(Pan and Rubin, 1998)

Heterozygotes display minaturized maxillary palps, characterized by fewer bristles.

(Bhojwani et al., 1997)

The trunk segments of homozygous larvae are reduced in size compared to wild-type. The Keilin's organs fail to develop. Partial rescue of the abdominal segment and Keilin's organ phenotypes are seen if the flies also carry tsh^mCa, tsh^220.mCa or tsh^bcd.mCa, with the greatest rescue occurring with flies carrying tsh^220.mCa.

(Core et al., 1997)

tsh⁸/tsh⁰⁴³¹⁹ and tsh⁸/tsh^B4-2-12 heterozygotes are lethal. Weak tsh phenotype (halteres droop, wings held up) when heterozygous with tsh^4-3. There are some escapers when heterozygous with tsh⁵¹.

(Kerridge, 1996.11.6)

Mutant embryos fail to form the central constriction and often contain a misplaced anterior constriction. In 87% of the mutant animals an indentation forms close to the gastric caeca; occasionally a small anterior compartment is evident. In 13% of embyos an apparently normal anterior constriction forms.

(Mathies et al., 1994)

Scr⁴, tsh⁸ double mutants develop the dorsal part of the thoracic trunk segment, whereas tsh⁸ mutants do not.

(Roder et al., 1992)

Trunk ventral denticle belts are disorganized. Dorsal and ventral parts of the prothorax are deleted. Ectopic papilla-like sense organs differentiate in the thorax region. The anal opening is sclerotized. Neuronal defects: disruption or deletion of ventral groups of neurons in the trunk region and misrouting of axons towards the head.

(Fasano et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tsh⁸ has visible | homeotic phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^69B

(Laugier et al., 2005)

tsh⁸ has visible | segment polarity expression pattern phenotype, suppressible by Scer\GAL4^69B/Mmus\Tshz2^UAS.Tag:MYC

(Manfroid et al., 2004)

tsh⁸ has visible | segment polarity expression pattern phenotype, suppressible by Mmus\Tshz3^UAS.Tag:MYC/Scer\GAL4^69B

(Manfroid et al., 2004)

tsh⁸ has visible | segment polarity expression pattern phenotype, suppressible by Mmus\Tshz1^UAS.Tag:MYC/Scer\GAL4^69B

(Manfroid et al., 2004)

Enhancer of

Statement

Reference

tsh⁸ is an enhancer of visible phenotype of Antp^73b

(Bhojwani et al., 1997)

Other

Statement

Reference

Antp^73b, tsh⁸ has visible | dominant phenotype

(Bhojwani et al., 1997)

Cul2⁰²⁰⁷⁴, tsh⁸ has viable phenotype

(Kerridge, 1996.11.6)

l(2)03832a^03832a, tsh⁸ has viable phenotype

(Kerridge, 1996.11.6)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, tsh⁸ has embryonic abdominal segment phenotype, enhanceable by disco^M.UAS, Scer\GAL4^prd.RG1

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, tsh⁸ has embryonic thoracic segment phenotype, enhanceable by disco^M.UAS, Scer\GAL4^prd.RG1

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, tsh⁸ has cirri | ectopic phenotype, enhanceable by disco^M.UAS, Scer\GAL4^prd.RG1

(Robertson et al., 2004)

tsh⁸ has ventral denticle belt phenotype, enhanceable by brk^M68

(Saller et al., 2002)

Suppressed by

Statement

Reference

tsh⁸ has denticle row 3 phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^69B

(Laugier et al., 2005)

tsh⁸ has denticle row 4 phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^69B

(Laugier et al., 2005)

tsh⁸ has prothoracic segment phenotype, suppressible by tio^UAS.cLa/Scer\GAL4^69B

(Laugier et al., 2005)

tsh⁸ has embryonic labial segment | ectopic phenotype, suppressible by Mmus\Tshz3^UAS.Tag:MYC/Scer\GAL4^69B

(Manfroid et al., 2004)

tsh⁸ has embryonic prothoracic segment phenotype, suppressible by Mmus\Tshz1^UAS.Tag:MYC/Scer\GAL4^69B

(Manfroid et al., 2004)

tsh⁸ has embryonic prothoracic segment phenotype, suppressible by Scer\GAL4^69B/Mmus\Tshz2^UAS.Tag:MYC

(Manfroid et al., 2004)

tsh⁸ has embryonic prothoracic segment phenotype, suppressible by Mmus\Tshz3^UAS.Tag:MYC/Scer\GAL4^69B

(Manfroid et al., 2004)

tsh⁸ has embryonic/first instar larval cuticle phenotype, suppressible by Mmus\Tshz1^UAS.Tag:MYC/Scer\GAL4^69B

(Manfroid et al., 2004)

tsh⁸ has embryonic/first instar larval cuticle phenotype, suppressible by Scer\GAL4^69B/Mmus\Tshz2^UAS.Tag:MYC

(Manfroid et al., 2004)

tsh⁸ has embryonic/first instar larval cuticle phenotype, suppressible by Mmus\Tshz3^UAS.Tag:MYC/Scer\GAL4^69B

(Manfroid et al., 2004)

tsh⁸ has embryonic labial segment | ectopic phenotype, suppressible by Mmus\Tshz1^UAS.Tag:MYC/Scer\GAL4^69B

(Manfroid et al., 2004)

tsh⁸ has embryonic labial segment | ectopic phenotype, suppressible by Scer\GAL4^69B/Mmus\Tshz2^UAS.Tag:MYC

(Manfroid et al., 2004)

NOT suppressed by

Statement

Reference

tsh⁸ has denticle row 1 phenotype, non-suppressible by tio^UAS.cLa/Scer\GAL4^69B

(Laugier et al., 2005)

tsh⁸ has denticle row 2 phenotype, non-suppressible by tio^UAS.cLa/Scer\GAL4^69B

(Laugier et al., 2005)

Enhancer of

Statement

Reference

tsh⁸ is an enhancer of embryonic abdominal segment phenotype of Dfd^UAS.cBa, Scer\GAL4^prd.RG1

(Robertson et al., 2004)

tsh⁸ is an enhancer of embryonic thoracic segment phenotype of Dfd^UAS.cBa, Scer\GAL4^prd.RG1

(Robertson et al., 2004)

tsh⁸ is an enhancer of cirri | ectopic phenotype of Dfd^UAS.cBa, Scer\GAL4^prd.RG1

(Robertson et al., 2004)

tsh⁸ is an enhancer of ventral denticle belt phenotype of brk^M68

(Saller et al., 2002)

tsh⁸ is an enhancer of antenna phenotype of Antp^73b

(Bhojwani et al., 1997)

tsh⁸ is an enhancer of leg | ectopic phenotype of Antp^73b

(Bhojwani et al., 1997)

Suppressor of

Statement

Reference

tsh⁸ is a suppressor of embryonic labial segment | ectopic phenotype of gt^Q292

(Sanders et al., 2008)

tsh⁸ is a suppressor of embryonic/first instar larval cuticle phenotype of Scer\GAL4^69B, arm^{S10.UAS.Tag:MYC}

(Gallet et al., 1998)

Other

Statement

Reference

Scer\GAL4^69B, tio^UAS.cLa, tsh⁸ has larval head segment & denticle | ectopic phenotype

(Laugier et al., 2005)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, tsh⁸ has mouth hook | ectopic phenotype

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^arm.PS, disco^M.UAS, tsh⁸ has mouth hook | ectopic phenotype

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, disco^M.UAS, tsh⁸ has mouth hook | ectopic phenotype

(Robertson et al., 2004)

Scer\GAL4^αTub84B.PP, ci^UAS.cAa, tsh⁸ has ventral denticle belt phenotype

(Angelats et al., 2002)

Scer\GAL4^αTub84B.PP, ci^UAS.cAa, tsh⁸ has embryonic/first instar larval cuticle phenotype

(Angelats et al., 2002)

ci⁹⁴, tsh⁸ has embryonic/first instar larval cuticle phenotype

(Gallet et al., 2000)

Scer\GAL4^prd.RG1, hh^UAS.cIa, tsh⁸ has ventral denticle belt phenotype

(Gallet et al., 2000)

Antp^73b, tsh⁸ has antenna phenotype

(Bhojwani et al., 1997)

Scr^hs.PZ, tsh⁸ has embryonic primordium of adult salivary gland phenotype

(Jullien et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos hemizygous for gt^Q292 and homozygous for tsh⁸ show normal lobe-like morphology of the labial segment. The dorsal ridge is enlarged, but is separate from the labial segment in late-stage mutant embryos.

(Sanders et al., 2008)

Expression of tio^Scer\UAS.cLa, under the control of Scer\GAL4^69B, partially rescues the denticle row phenotype of tsh⁸ mutants at 25^oC, but fails to rescue this phenotype at 22^oC. At 25^oC, the partially-rescued larvae have five denticle rows, but types 1 and 2 are missing and types 3-6 are not in the same sequential order seen in wild-type larvae. The identity of the T1 segment is restored, shown by the presence of a T1 beard. However, expression of tio^Scer\UAS.cLa causes a new phenotype: ectopic denticles appear in the head region.

(Laugier et al., 2005)

The addition of ci^Scer\UAS.cAa (driven by Scer\GAL4^αTub84B.PP) to tsh⁸ mutant embryos, can suppress denticle belt formation in the thorax and the abdomen.

(Angelats et al., 2002)

brk^M68 tsh⁸ double mutant larvae show an essentially naked cuticle phenotype, with only single tiny denticles occasionally seen scattered in the posterior abdomen.

(Saller et al., 2002)

The denticle types present (5 and 6) in tsh⁸ mutant embryos are unchanged if they are also expressing hh^Scer\UAS.cIa under the control of Scer\GAL4^prd.RG1, although in some segments the denticle belts are reduced and the shape of the denticle belt is modified. Cuticle from tsh⁸ ; ci⁹⁴ double mutant embryos produces only weakly pigmented, type 5 denticles and no naked cuticle.

(Gallet et al., 2000)

The naked cuticle phenotype produced by arm^{S10.Scer\UAS.T:Hsap\MYC} expressed under the control of Scer\GAL4^69B is suppressed by tsh⁸.

(Gallet et al., 1998)

Antenna to leg transformation is seen in an Antp^73b mutant background.

(Bhojwani et al., 1997)

tsh⁸ embryos expressing Scr^hs.PZ develop supernumerary salivary glands in parasegments PS0-PS1 and in PS3-PS13.

(Jullien et al., 1997)

tsh⁸/cul-2⁰²⁰⁷⁴ and tsh⁸/l(2)03832a^03832a heterozygotes are viable.

(Kerridge, 1996.11.6)

In the presence of construct P{HS-Scr.Z} ectopic salivary glands do not form in parasegment 3.

(Andrew et al., 1994)

Xenogenetic Interactions

Statement

Reference

At 22^oC, the presence of Mmus\Sdccag33^{Scer\UAS.T:Hsap\MYC} and Scer\GAL4^69B results in partial rescue of the T1 segment and trunk cuticle phenotypes of tsh⁸/tsh⁸ embryos. At 29^oC this rescue is more complete. At 22^oC, the presence of Mmus\Sdccag33l^{Scer\UAS.T:Hsap\MYC} and Scer\GAL4^69B leads to little or no rescue of the segment transformations apparent in the cuticle phenotype of tsh⁸/tsh⁸ embryos. However, at 29^oC this phenotype is partially rescued. At 22^oC, the presence of Mmus\Zfp537^{Scer\UAS.T:Hsap\MYC} and Scer\GAL4^69B results in partial rescue of the T1 segment and trunk cuticle phenotypes of tsh⁸/tsh⁸ embryos. All these larvae show significant but incomplete rescue of the T1 denticle belt phenotype but none of them displays beard-specific denticles. The size of the naked cuticle bands in the trunk appears normal. At 29^oC this rescue is more complete.

(Manfroid et al., 2004)

Complementation and Rescue Data

Complements

tsh^4-3

(Soanes and Bell, 1999)

tsh⁵¹

(Soanes and Bell, 1999)

Fails to complement

tsh⁰⁴³¹⁹

(Soanes and Bell, 1999)

tsh^B4-2-12

(Soanes and Bell, 1999)

tsh¹³⁴

(Bhojwani et al., 1995)

tsh¹⁷

(Bhojwani et al., 1995)

tsh⁸²

(Bhojwani et al., 1995)

Rescued by

tsh⁸ is rescued by tsh^+t28.4

(Venken et al., 2006)

tsh⁸ is rescued by tsh^+t86.4

(Venken et al., 2006)

Partially rescued by

tsh⁸ is partially rescued by tsh^UAS.cGa/Scer\GAL4^69B

(Laugier et al., 2005)

tsh⁸ is partially rescued by tsh^mCa

(Core et al., 1997)

tsh⁸ is partially rescued by tsh^bcd.mCa

(Core et al., 1997)

tsh⁸ is partially rescued by tsh^220.mCa

(Core et al., 1997)

tsh⁸ is partially rescued by tsh^hs.PdZ

(de Zulueta et al., 1994)

Comments

Expression of tsh^Scer\UAS.cGa at 22^oC, under the control of Scer\GAL4^69B, rescues the denticle and naked cuticle phenotype of tsh⁸ mutants and Ti segment identity is restored. However, this expression induces ectopic denticle formation in the head region.

(Laugier et al., 2005)

Phenotype is partially rescued by heat shock treatment of embryos carrying tsh^hs.PdZ.

(de Zulueta et al., 1994)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

50763

tsh⁸/CyO, P{en1}wg^en11

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Df(2L)tsh8

(Soanes and Bell, 1999, Kerridge, 1996.11.6)

Df(2L)tsh⁸

(Venken, 2006, Laugier et al., 2005, Andrew et al., 1994)

tsh8

(Chauvet et al., 2000)

tsh⁸

(Banerjee et al., 2025, Rubio-Ferrera et al., 2023, Clarembaux-Badell et al., 2022, Kahsai et al., 2016, Denholm et al., 2013, Oros et al., 2010, Sanders et al., 2008, Taghli-Lamallem et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (40)

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