FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\CycEUAS.cRa
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General Information
Symbol
Dmel\CycEUAS.cRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Richardson
FlyBase ID
FBal0044719
Feature type
allele
Associated gene
Dmel\CycE
Associated Insertion(s)
Carried in Construct
P{UAS-CycE.R}
Also Known As
UAS-CycE, UAS-cyclin E
Key Links
Transgenic product class
cDNA
(Richardson et al., 1995)
wild_type
(Richardson et al., 1995)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Richardson et al., 1995)
wild_type
(Richardson et al., 1995)
Mutagen
in vitro construct
(Richardson et al., 1995)
Progenitor genotype
Carried in construct
P{UAS-CycE.R}
Cytology
Description

CycE type I cDNA (coordinates 415-2748 of FBrf0065548) is expressed from UASt regulatory sequences.

(Richardson et al., 1995)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
CycE
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  cancer
      CEA
      (Molano-Fernández et al., 2022)
      model of  prostate cancer
      CEA
      (Sekar et al., 2023)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expressing CycEUAS.cRa under the control of Scer\GAL4ptc.PU leads to a significant increase in size of the wing blade domain between L3 and L4 veins.

      (Kim and Choi, 2019)

      Shortening the G[[1]] cell cycle phase in imaginal disc cells, through expression of CycEScer\UAS.cRa under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU, does not result in an increase in DNA damage and cell death, compared to wild-type.

      (Wong et al., 2014)

      Expression of CycEScer\UAS.cRa in the developing eye, driven by Scer\GAL4GMR.PF, results in a slight increase in the number of ommatidial cells.

      (Nolo et al., 2006)

      Expression of CycEScer\UAS.cRa in patches of the embryonic salivary gland, driven by Scer\GAL4prd.RG1, blocks endoreplication of salivary gland cells. This does not affect salivary gland invagination.

      (Chandrasekaran and Beckendorf, 2005)

      In 11% of hemisegments in CycEScer\UAS.cRa; Scer\GAL4ftz.ng embryos the neuroblasts that give rise to RP2 neurons (neuroblast NB4-2 ?) divide to produce one RP2 or RP2 sib plus a new ganglion mother cell (ectopic neuroblast NB4-2 ?), which then divides again to produce one RP2 neuron and one RP2 sib. These embryos also have hemisegments with two pCCs and one aCC or two aCCs and one pCC, suggesting a similarly abnormal pattern of divisions in the development of these neurons as well.

      (Bhat and Apsel, 2004)

      Scer\GAL443B-mediated expression greatly reduces the size of third instar salivary glands (they remain small), without noticeably compromising larval growth or survival. Continuous expression causes a prolonged period of replication inhibition in larvae.

      (Follette et al., 1998)

      Eye structure is disorganised, with blistered ommatidia and duplicated bristles.

      (Richardson et al., 1995)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Suppressor of
      Statement
      Reference

      Scer\GAL4spok.1.45/CycEUAS.cRa is a suppressor of abnormal endomitotic cell cycle | third instar larval stage phenotype of Kdm5140

      (Drelon et al., 2019)

      Scer\GAL4spok.1.45/CycEUAS.cRa is a suppressor of abnormal developmental rate phenotype of Kdm5140

      (Drelon et al., 2019)

      CycEUAS.cRa, Scer\GAL4ptc.PU is a suppressor | partially of abnormal size | adult stage phenotype of CCT4KK108551, Scer\GAL4ptc.PU

      (Kim and Choi, 2019)

      CycEUAS.cRa, Scer\GAL4ptc.PU is a suppressor | partially of abnormal size | adult stage phenotype of CCT4NIG.5525R, Scer\GAL4ptc.PU

      (Kim and Choi, 2019)

      CycEUAS.cRa, Scer\GAL4en-e16E is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4en-e16E, Skp2KK108837

      (Dui et al., 2013)

      stghs.PE2/CycEUAS.cRa, Scer\GAL4en.PU is a suppressor of increased cell growth rate phenotype of Ras85DV12.UAS, Scer\GAL4en.PU, dlg1GD4689

      (Willecke et al., 2011)

      CycEUAS.cRa, Scer\GAL4ey.PH is a suppressor | partially of visible phenotype of Mmus\Shisa5UAS.cGa, Scer\GAL4ey.PH

      (Gupta et al., 2008)

      CycEUAS.cRa, Scer\GAL4ey.PH is a suppressor | partially of abnormal size phenotype of Mmus\Shisa5UAS.cGa, Scer\GAL4ey.PH

      (Gupta et al., 2008)

      CycEUAS.cRa, Scer\GAL4dpp.PH is a suppressor | partially of visible phenotype of Mmus\Shisa5UAS.cGa, Scer\GAL4dpp.PH

      (Gupta et al., 2008)

      CycEUAS.cRa, Scer\GAL4dpp.PH is a suppressor | partially of increased cell death phenotype of Mmus\Shisa5UAS.cGa, Scer\GAL4dpp.PH

      (Gupta et al., 2008)

      CycEUAS.cRa, Scer\GAL4αTub84B.PL, BacA\p35UAS.cHa is a suppressor of cell lethal | somatic clone phenotype of scrib1

      (Brumby and Richardson, 2003)
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      BacA\p35GMR.PH, CycEUAS.cRa, Scer\GAL4GMR.PF has eye phenotype, enhanceable by brm25S14

      (Brumby et al., 2002)

      BacA\p35GMR.PH, CycEUAS.cRa, Scer\GAL4GMR.PF has eye phenotype, enhanceable by mor35S1

      (Brumby et al., 2002)
      Suppressed by
      Statement
      Reference

      CycEUAS.cRa, Scer\GAL4ptc.PU has wing blade phenotype, suppressible | partially by CCT4KK108551, Scer\GAL4ptc.PU

      (Kim and Choi, 2019)
      NOT suppressed by
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      CycEUAS.cRa, Scer\GAL4bbg-C96 is a non-suppressor of wing phenotype of Mnat9KK109382, Scer\GAL4bbg-C96

      (Mok and Choi, 2021)

      CycEUAS.cRa, Scer\GAL4bbg-C96 is a non-suppressor of wing phenotype of Mmus\Shisa5UAS.cGa, Scer\GAL4bbg-C96

      (Gupta et al., 2008)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Shortening the G[[1]] cell cycle phase in imaginal disc cells, through expression of CycEScer\UAS.cRa under the control of Scer\GAL4en.PU or Scer\GAL4hh.PU, does not result in an increase in DNA damage and cell death when chromosomal instability is induced by co-expression of mad2GD16427.

      (Wong et al., 2014)

      Co-expression of CycEScer\UAS.cRa almost completely suppresses the wing hair spacing phenotype seen when Skp2KK108837 is expressed under the control of Scer\GAL4en-e16E.

      (Dui et al., 2013)

      Wing disc compartments expressing Ras85DV12.Scer\UAS, dlg1GD4689, Pi3K92EGD11228, together with CycEScer\UAS.cRa and stghs.PE2 show a rescue in compartment size and high levels of His3-positive cells.

      (Willecke et al., 2011)

      Expression of CycEScer\UAS.cRa under the control of Scer\GAL4tub results in four times more Tctph59 cells being present in clones 72 hours after their induction.

      (Hsu et al., 2007)

      Coexpression of thScer\UAS.cHa and CycEScer\UAS.cRa in the developing eye, under the control of Scer\GAL4GMR.PF, results in the generation of many small ommatidial cells.

      Coexpression of CycEScer\UAS.cRa with banEP3622, under the control of Scer\GAL4GMR.PF, enhances the extra ommatidial cell phenotype seen in Scer\GAL4GMR.PF>banEP3622 pupal retinae.

      Coexpression of banEP3622, CycEScer\UAS.cRa and thScer\UAS.cHa, under the control of Scer\GAL4GMR.PF, causes a large increase in interommatidial, cone and photoreceptor cells.

      (Nolo et al., 2006)

      Expression of CycEScer\UAS.cRa, under the control of Scer\GAL4prd.RG1, rescues the long salivary gland phenotype either completely or partially in 69% of Btk29Ak00206 embryos.

      (Chandrasekaran and Beckendorf, 2005)

      CycEScer\UAS.cRa when driven by Scer\GAL4GMR.PF causes eye overgrowth and rough eye phenotype. Addition of brm25S14 or mor35S1 enhances this phenotype.

      (Brumby et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference

      Expression of CycEScer\UAS.cRa significantly suppresses the eye phenotypes seen when Mmus\Shisa5Scer\UAS.cGa is expressed under the control of Scer\GAL4ey.PH.

      Expression of CycEScer\UAS.cRa significantly suppresses the wing phenotypes seen when Mmus\Shisa5Scer\UAS.cGa is expressed under the control of Scer\GAL4dpp.PH. The amount of apoptosis seen in the wing disc is also significantly reduced.

      Expression of CycEScer\UAS.cRa does not suppress the wing phenotypes seen when Mmus\Shisa5Scer\UAS.cGa is expressed under the control of Scer\GAL4bbg-C96. The amount of apoptosis seen in the wing disc is not reduced.

      (Gupta et al., 2008)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      CycEScer\UAS.cRa
      CycEUAS.cRa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Richardson
      (Richardson et al., 1995)
      Secondary FlyBase IDs
        References (24)