FlyBase Allele Report: Dmel\hep[UAS.cBa]

Expression of hep^UAS.cBa under the control of Scer\GAL4^esg.PU, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, results in an increase in progenitor cells and stem cell division, as well as distortion of the epithelial architecture in the gut, compared to controls; adult-stage expression under the control of Scer\GAL4^NP0001 results in reduced numbers of enterocytes in the gut, but a significant increase in stem cell division and increased numbers of pre-enterocytes, compared to controls.

(Mundorf et al., 2019)

Expressing hep^UAS.cBa under the control of Scer\GAL4^ptc.PU induces some cells at the wing disc A/P boundary to delaminate and migrate to the posterior compartment, as well as cell death at the boundary (acridine orange staining). Expression under the control of Scer\GAL4^GMR.PU also induces cell death, but not cell invasion, in the eye disc.

(Sun et al., 2019)

The expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 does not lead to obvious size defects in third instar larval wing discs, as compared to controls.

(Bunker et al., 2015)

Expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^esg-NP5130 dramatically increases the number of mitotic cells in the adult gut.

(Meng and Biteau, 2015)

Expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^esg-NP5130 (using tub-gal80[ts] to limit the time of expression) increases cell proliferation in the adult gut.

(Wang et al., 2015)

Expression of hep^Scer\UAS.cBa driven by Scer\GAL4^pnr-MD237 triggers cell death in the thorax and produces a small-scutellum phenotype.

Expression of hep^Scer\UAS.cBa along the anterior-posterior compartment boundary driven by Scer\GAL4^ptc-559.1 induces elevated cell death in third instar wing discs relative to wild-type and results in the loss of the anterior crossvein.

(Zhang et al., 2015)

The temporally regulated expression (by Gal80[ts]) of hep^UAS.cBa, for 4 or 14 days under the control of Scer\GAL4^NP5130, leads to strong increases in the proportion of mitotic cells and in the number of intestinal stem cells (assessed by immuno-staining for Delta) in the adult midgut, as compared to controls.

(Wang et al., 2014)

Expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG results in delayed or defective ventral furrow formation in a proportion of embryos.

(Mathew et al., 2011)

Transient overexpression of hep^Scer\UAS.cBa in adults under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B induces increased proliferation of intestinal cells compared with controls.

Transient overexpression of hep^Scer\UAS.cBa in adults under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B results in significant lifespan shortening compared with wild-type.

(Biteau et al., 2010)

Expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^ptc-559.1 results in a dramatic increase in the number of intercalated cells and the formation of ectopic mixer cells at the segment boundaries during dorsal closure (in the abdominal segments of wild-type embryos at the end of dorsal closure, a mixer cell moves across the segment boundary from the anterior compartment to the posterior compartment and two cells from the ventral ectoderm intercalate into the leading edge, posterior to the mixer cell).

(Gettings et al., 2010)

Ectopic expression of hep^Scer\UAS.cBa driven by Scer\GAL4^69B in embryos leads to cuticles with openings in the anterior part and puckering in the dorsal part.

Ectopic expression of hep^Scer\UAS.cBa driven by either Scer\GAL4^Abd-B-LDN or Scer\GAL4^ptc-559.1 leads to male genital disc effects such as incomplete rotation and separation of the plate from the abdomen.

(Rousset et al., 2010)

Animals expressing hep^Scer\UAS.cBa under the control of Scer\GAL4^ppl.PP show increased tolerance to paraquat compared to controls.

(Hull-Thompson et al., 2009)

hep^Scer\UAS.cBa Scer\GAL4^GMR.PF adults have normal eyes.

(Wang et al., 2005)

Expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF does not affect eye development.

(Cha et al., 2003)

Expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^GMR.PF has no effect on eye morphology.

(Shulman and Feany, 2003)

Flies expressing hep^Scer\UAS.cBa, under the control of Scer\GAL4^arm.PS, show a decreased sensitivity to paraquat compared to wild-type flies. This decreased sensitivity is also seen when hep^Scer\UAS.cBa is expressed under the control of the neuron-specific driver Scer\GAL4^{elav.Switch.PO}, but only in the presence of RU486. However, expression of hep^Scer\UAS.cBa under the control of the muscle-specific driver Scer\GAL4^{Mhc.Switch.PO} does not affect tolerance to paraquat. Overexpression of hep^Scer\UAS.cBa, driven by Scer\GAL4^elav-C155, increases the lifespan of flies.

(Wang et al., 2003)

When expression is driven by Scer\GAL4^hs.2sev, flies have rough eyes showing polarity phenotypes. Ommatidia are incorrectly rotated and sometimes exhibit the wrong chiral form.

(Weber et al., 2000)

Adults in which hep^Scer\UAS.cBa is expressed under the control of Scer\GAL4^pnr-MD237 have a severely reduced scutellum, and there is a cleft in the tissue at the dorsal midline. The bristles are oriented in a different direction from normal, are loosely spaced and sometimes have kinks.

(Zeitlinger and Bohmann, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ptc.PU, hep^UAS.cBa has abnormal cell migration | third instar larval stage phenotype, enhanceable by lic^UAS.ORF/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^ptc.PU, hep^UAS.cBa has abnormal cell migration | third instar larval stage phenotype, enhanceable by Hsap\MAP2K3^UAS.cSa, Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has visible phenotype, enhanceable by puc^E69

(Zeitlinger and Bohmann, 1999)

Suppressed by

Statement

Reference

Scer\GAL4^esg.PU, hep^UAS.cBa has increased occurrence of cell division | adult stage phenotype, suppressible by Ets21C^KK103211, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Scer\GAL4^esg.PU, hep^UAS.cBa has increased occurrence of cell division | adult stage phenotype, suppressible by Ets21C^RNAi.UAS.cMa, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Scer\GAL4^esg.PU, hep^UAS.cBa has increased cell number | adult stage phenotype, suppressible by Ets21C^KK103211, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Scer\GAL4^esg.PU, hep^UAS.cBa has increased cell number | adult stage phenotype, suppressible by Ets21C^RNAi.UAS.cMa, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Scer\GAL4^NP0001, hep^UAS.cBa has increased occurrence of cell division | adult stage phenotype, suppressible by Ets21C^KK103211, Scer\GAL4^NP0001

(Mundorf et al., 2019)

Scer\GAL4^NP0001, hep^UAS.cBa has abnormal cell number | adult stage phenotype, suppressible by Ets21C^KK103211, Scer\GAL4^NP0001

(Mundorf et al., 2019)

Scer\GAL4^NP5130, hep^UAS.cBa has increased occurrence of cell division phenotype, suppressible by PEK^GD5584, Scer\GAL4^NP5130

(Wang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has abnormal size phenotype, suppressible | partially by wg[+]/wg^l-8

(Zhang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has visible phenotype, suppressible | partially by wg[+]/wg^l-8

(Zhang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has abnormal size phenotype, suppressible | partially by dsh³/dsh[+]

(Zhang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has visible phenotype, suppressible | partially by dsh³/dsh[+]

(Zhang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has abnormal size phenotype, suppressible | partially by arm²/arm[+]

(Zhang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has visible phenotype, suppressible | partially by arm²/arm[+]

(Zhang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has visible phenotype, suppressible by Scer\GAL4^ptc-559.1/dsh^RNAi.UAS.cUa

(Zhang et al., 2015)

Scer\GAL4^ptc-559.1, hep^UAS.cBa has increased cell death | third instar larval stage phenotype, suppressible by dsh³/dsh[+]

(Zhang et al., 2015)

Scer\GAL4^ptc-559.1, hep^UAS.cBa has increased cell death | third instar larval stage phenotype, suppressible by Scer\GAL4^ptc-559.1/dsh^RNAi.UAS.cUa

(Zhang et al., 2015)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc.PU, hep^UAS.cBa has abnormal cell migration | third instar larval stage phenotype, non-suppressible by lic^GD7546/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Enhancer of

Statement

Reference

hep^UAS.cBa, Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Daxx^EY09290, Scer\GAL4^Bx-MS1096

(Hwang et al., 2013)

hep^UAS.cBa, Scer\GAL4^Act5C.PI is an enhancer of neoplasia | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act5C.PI

(Uhlirova and Bohmann, 2006)

hep^UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, by^UAS.cLa

(Lee et al., 2003)

hep^UAS.cBa, Scer\GAL4^GMR.PF is an enhancer of visible phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

hep^UAS.cBa/Scer\GAL4^elav.PU is a suppressor of abnormal oxidative stress response phenotype of ben¹

(Ma et al., 2014)

hep^UAS.cBa/Scer\GAL4^elav.PU is a suppressor of short lived phenotype of ben¹

(Ma et al., 2014)

hep^UAS.cBa/Scer\GAL4^ey-OK107 is a suppressor of abnormal neuroanatomy | somatic clone phenotype of Mkk4^e01485

(Rallis et al., 2010)

hep^UAS.cBa/Scer\GAL4^ato.3.6 is a suppressor of abnormal neuroanatomy phenotype of dsh¹

(Srahna et al., 2006)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, aPKC^{UAS.Tag:CAAX(Unk)}, hep^UAS.cBa has abnormal size | third instar larval stage phenotype

(Bunker et al., 2015)

Scer\GAL4^Bx-MS1096, aPKC^{UAS.Tag:CAAX(Unk)}, hep^UAS.cBa has abnormal cell polarity | third instar larval stage phenotype

(Bunker et al., 2015)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ptc.PU, hep^UAS.cBa has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, enhanceable by lic^UAS.ORF/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^ptc.PU, hep^UAS.cBa has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, enhanceable by Hsap\MAP2K3^UAS.cSa, Scer\GAL4^ptc.PU

(Sun et al., 2019)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has scutellum phenotype, enhanceable by puc^E69

(Zeitlinger and Bohmann, 1999)

Suppressed by

Statement

Reference

Scer\GAL4^esg.PU, hep^UAS.cBa has adult midgut epithelium phenotype, suppressible by Ets21C^KK103211, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Scer\GAL4^esg.PU, hep^UAS.cBa has adult midgut epithelium phenotype, suppressible by Ets21C^RNAi.UAS.cMa, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Scer\GAL4^esg.PU, hep^UAS.cBa has intestinal stem cell phenotype, suppressible by Ets21C^KK103211, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Scer\GAL4^esg.PU, hep^UAS.cBa has intestinal stem cell phenotype, suppressible by Ets21C^RNAi.UAS.cMa, Scer\GAL4^esg.PU

(Mundorf et al., 2019)

Scer\GAL4^NP0001, hep^UAS.cBa has adult midgut epithelium phenotype, suppressible | partially by Ets21C^KK103211, Scer\GAL4^NP0001

(Mundorf et al., 2019)

Scer\GAL4^NP0001, hep^UAS.cBa has intestinal stem cell phenotype, suppressible by Ets21C^KK103211, Scer\GAL4^NP0001

(Mundorf et al., 2019)

Scer\GAL4^NP0001, hep^UAS.cBa has adult midgut enterocyte phenotype, suppressible by Ets21C^KK103211, Scer\GAL4^NP0001

(Mundorf et al., 2019)

Scer\GAL4^NP5130, hep^UAS.cBa has adult gut phenotype, suppressible by PEK^GD5584, Scer\GAL4^NP5130

(Wang et al., 2015)

Scer\GAL4^ptc-559.1, hep^UAS.cBa has anterior crossvein phenotype, suppressible by Scer\GAL4^ptc-559.1/dsh^RNAi.UAS.cUa

(Zhang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has scutellum phenotype, suppressible | partially by wg[+]/wg^l-8

(Zhang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has scutellum phenotype, suppressible | partially by dsh³/dsh[+]

(Zhang et al., 2015)

Scer\GAL4^pnr-MD237, hep^UAS.cBa has scutellum phenotype, suppressible | partially by arm²/arm[+]

(Zhang et al., 2015)

Scer\GAL4^ptc-559.1, hep^UAS.cBa has anterior crossvein phenotype, suppressible | partially by dsh³/dsh[+]

(Zhang et al., 2015)

Scer\GAL4^NP5130, hep^UAS.cBa has midgut | adult stage phenotype, suppressible by Xbp1^UAS.RB/Scer\GAL4^NP5130

(Wang et al., 2014)

Scer\GAL4^twi.PG, hep^UAS.cBa has ventral furrow phenotype, suppressible | germline clone by bsk^unspecified

(Mathew et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ptc.PU, hep^UAS.cBa has anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype, non-suppressible by lic^GD7546/Scer\GAL4^ptc.PU

(Sun et al., 2019)

Enhancer of

Statement

Reference

hep^UAS.cBa, Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Daxx^EY09290, Scer\GAL4^Bx-MS1096

(Hwang et al., 2013)

hep^UAS.cBa/Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, foxo^UAS.cPa

(Wang et al., 2005)

hep^UAS.cBa/Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, by^UAS.cLa

(Lee et al., 2003)

hep^UAS.cBa, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

hep^UAS.cBa, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Shulman and Feany, 2003)

Suppressor of

Statement

Reference

hep^UAS.cBa/Scer\GAL4^ey-OK107 is a suppressor of Kenyon cell | somatic clone phenotype of Mkk4^e01485

(Rallis et al., 2010)

hep^UAS.cBa/Scer\GAL4^ey-OK107 is a suppressor of axon | somatic clone phenotype of Mkk4^e01485

(Rallis et al., 2010)

hep^UAS.cBa/Scer\GAL4^ato.3.6 is a suppressor of axon & dorsal cluster neuron phenotype of dsh¹

(Srahna et al., 2006)

hep^UAS.cBa/Scer\GAL4^VP16.arm is a suppressor of embryonic epidermis | dorsal phenotype of arm³

(McEwen et al., 2000)

hep^UAS.cBa/Scer\GAL4^VP16.arm is a suppressor of embryonic epidermis | ventral phenotype of arm³

(McEwen et al., 2000)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, aPKC^{UAS.Tag:CAAX(Unk)}, hep^UAS.cBa has wing disc | third instar larval stage phenotype

(Bunker et al., 2015)

Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, hep^UAS.cBa has larval brain | somatic clone phenotype

(Uhlirova and Bohmann, 2006)

Ras85D^V12.UAS, Scer\GAL4^Act5C.PI, hep^UAS.cBa has larval ventral nerve cord | somatic clone phenotype

(Uhlirova and Bohmann, 2006)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of hep^Scer\UAS.cBa and aPKC^{CAAXWT.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 leads to moderate overgrowth and cell polarity defects in third instar larval wing discs, as compared to controls.

(Bunker et al., 2015)

The increased cell proliferation observed upon expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^esg-NP5130 in the adult gut is suppressed by co-expression of either Sox21a^JF02191 or kay^{dsRNA.Scer\UAS}.

(Meng and Biteau, 2015)

The increased cell proliferation in the adult gut expressing hep^Scer\UAS.cBa under the control of Scer\GAL4^esg-NP5130 (using tub-gal80[ts] to limit the time of expression) is suppressed by co-expression of PEK^GD5584.

(Wang et al., 2015)

Expression of hep^Scer\UAS.cBa under the control of Scer\GAL4^elav.PU fully rescues the reduced oxidative stress resistance and shortened lifespan seen in ben¹ mutant males.

(Ma et al., 2014)

The reduced wing phenotype caused by expression of DLP^EY09290 under the control of Scer\GAL4^Bx-MS1096 is strongly exacerbated by co-expression of hep^Scer\UAS.cBa.

(Hwang et al., 2013)

The defects in ventral furrow formation seen in embryos expressing hep^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG are completely suppressed in embryos derived from bsk^unspecified female germline clones.

(Mathew et al., 2011)

The axon overextension phenotype of Mkk4^e01485 neuroblast clones in mushroom bodies is suppressed by expression of hep^Scer\UAS.cBa using Scer\GAL4^ey-OK107.

(Rallis et al., 2010)

The increased tolerance of animals expressing hep^Scer\UAS.cBa under the control of Scer\GAL4^ppl.PP to paraquat is abolished by NLaz^NW5/NLaz^NW5, with the double mutant flies showing the same sensitivity to paraquat as NLaz^NW5/NLaz^NW5 single mutants.

(Hull-Thompson et al., 2009)

Expression of hep^Scer\UAS.cBa, under the control of Scer\GAL4^ato.3.6, in a dsh¹ background results in a large increase of dorsal cluster neuron axons crossing the optic chiasm, indicating a complete dominance of the hep gain of function phenotype.

(Srahna et al., 2006)

Coexpression of hep^Scer\UAS.cBa and Ras85D^V12.Scer\UAS under the control of Scer\GAL4^Act5C.PI results in eye/antennal imaginal disc clones results in the invasion of the disc cells into the ventral nerve cord and regions of the brain.

(Uhlirova and Bohmann, 2006)

The small, deformed eye phenotype of foxo^Scer\UAS.cPa; Scer\GAL4^GMR.PF adults is enhanced by the presence of hep^Scer\UAS.cBa.

(Wang et al., 2005)

When hep^Scer\UAS.cBa is coexpressed with one copy of Traf1^EP578, driven by Scer\GAL4^GMR.PF, the number of ommatidia and the size of the compound eye are reduced to similar levels seen when two copies of Traf1^EP578 are expressed.

Coexpression of Traf2^EP1516 with either hep^Scer\UAS.cBa, under the control of Scer\GAL4^GMR.PF, does not affect the development of eyes.

(Cha et al., 2003)

The phenotype caused when hep^Scer\UAS.cBa is expressed under the control of Scer\GAL4^pnr-MD237is enhanced by a single copy of puc^E69; the scutellum is completely missing.

(Zeitlinger and Bohmann, 1999)

Xenogenetic Interactions

Statement

Reference

The rough eye phenotype caused by expression of Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of hep^Scer\UAS.cBa; eye surface roughness is increased, eye size is decreased and necrotic black patches are formed in the eye.

(Shulman and Feany, 2003)

Complementation and Rescue Data

Rescues

hep^UAS.cBa/Scer\GAL4^ey-OK107 rescues hep^r39

(Rallis et al., 2010)

Comments

Expression of hep^Scer\UAS.cBa with Scer\GAL4^ey-OK107 rescues the axonal phenotypes seen in hep^r39 mushroom body clones.

(Rallis et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (2)