FlyBase Allele Report: Scer\GAL4[Gr66a.PD]

ScerGAL4^Gr66a.PD drives expression in a subset of gustatory receptor neurons in the labellum which do not overlap with those marked by NcraQF^Ir76b.1.5. The projections of the ScerGAL4^Gr66a.PD-positive neurons in the subesophageal ganglion show minimal overlap with projection of neurons labelled by NcraQF^Ir76b.1.5.

(Zhang et al., 2013)

Gr66a and Gr93a are co-expressed in the labellum and the pharynx.

(Lee et al., 2009)

ScerGAL4^Gr66a.PD is expressed in 8 to 22 cells of each labial palp, 8 cells of the first leg, 7 cells of the second leg and 6 cells of the third leg.

(Thorne et al., 2004)

Adult expression is observed in about 20 cells, including an average of 1.6 cells in the prothoracic leg, 8 in the labial palps and in the labral and cibarial sense organs.

(Bray and Amrein, 2003)

Adult expression is observed in about 30 gustatory sensilla in the prothoracic legs, labral and cibarial sense organs, and laterally through two rows of bristles in the labial palps. Gr66a-expressing neurons project to two distinct regions in the subesophageal gangion via the labial and accessory pharyngeal nerves.

(Dunipace et al., 2001)

Marker for

high salt/bitter-sensing neuron

(Zhang et al., 2013, Mitri et al., 2009)

Reflects expression of

Gr66a

(Zhang et al., 2013, Zhang et al., 2013, Lee et al., 2009, Miyamoto and Amrein, 2008, Thorne et al., 2004, Bray and Amrein, 2003, Hiroi et al., 2002, Dunipace et al., 2001)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

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Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Sang et al., 2024)

abnormal courtship behavior, with pain^GD2221

(Wang et al., 2011)

abnormal feeding behavior, with Ctet\tetX^TNT-LC.UAS

(Mitri et al., 2009)

abnormal feeding behavior, with hid^UAS.cZa

(Mitri et al., 2009)

abnormal feeding behavior, with mtt^RNAi.UAS, mtt^f06268

(Mitri et al., 2009)

abnormal feeding behavior, with rpr^UAS.cZa

(Mitri et al., 2009)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Shao et al., 2017, Aso et al., 2014)

abnormal locomotor behavior | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^0273-G4

(Stern et al., 2019)

abnormal memory | adult stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Shao et al., 2017)

abnormal neurophysiology | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Sang et al., 2024)

abnormal proboscis extension reflex, with OtopLa^KK103716

(Ganguly et al., 2021)

abnormal proboscis extension reflex, with OtopLa^KK112648

(Ganguly et al., 2021)

abnormal proboscis extension reflex, with ppk28^UAS.cCb

(Chen et al., 2010)

abnormal proboscis extension reflex | light conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Sang et al., 2024)

abnormal taste perception, with Ctet\tetX^TNT-LC.UAS

(Thorne et al., 2004)

abnormal taste perception, with OtopLa^KK103716

(Ganguly et al., 2021)

abnormal taste perception, with OtopLa^KK112648

(Ganguly et al., 2021)

abnormal taste perception | adult stage, with hid^UAS.cZa

(Rimal et al., 2019)

Phenotype Manifest In

large labellar taste bristle, with TrpA1^UAS.(B).cKa

(Kang et al., 2012)

short labellar taste bristle | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Sang et al., 2024)

short labellar taste bristle S-a, with hid^UAS.cZa

(Rimal et al., 2019)

short labellar taste bristle S-b, with hid^UAS.cZa

(Rimal et al., 2019)

Detailed Description

Statement

Reference

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 624 nm (red) light) of the combined set of Scer\GAL4^0273-G4 cells and Scer\GAL4^Gr66a.PD cells increases preference for areas with red light, compared to controls.

(Stern et al., 2019)

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 617 nm (red) light) of Scer\GAL4^Gr66a.PD neurons in adults results in a significant avoidance of being in the region of activation (illuminated region), compared to controls.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with 617 nm (red) light) of Scer\GAL4^Gr66a.PD neurons in adults in combination with a paired odor, results in a significant increase in short-term associative aversive olfactory memory, compared to controls.

(Shao et al., 2017)

Inhibition (using Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^Gr66a.PD cells in starved adult males does not affect preference for softer sucrose-containing agarose, compared to controls.

(Sánchez-Alcañiz et al., 2017)

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 617 nm (red) light) of Scer\GAL4^Gr66a.PD cells in adult females in an area of an arena significantly increases avoidance of that area, compared to controls; there are significant increases in angular speed and walking speed, compared to baseline, whereas no increase is seen in controls.

(Aso et al., 2014)

Inhibition (using Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^Gr66a.PD cells in adults does not affect aversive temperature or shock olfactory memory, compared to controls.

(Galili et al., 2014)

External Data

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Additional Comments

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Xenogenetic Interactions