FlyBase Gene Report: Dmel\NaCP60E

General Information

Symbol

Dmel\NaCP60E

Species

D. melanogaster

Name

Na channel protein 60E

Annotation Symbol

CG34405

Feature Type

protein_coding_gene

FlyBase ID

FBgn0085434

Gene Model Status

Current

Stock Availability

15 publicly available

Gene Summary

Na channel protein 60E (NaCP60E) encodes a voltage-gated calcium-selective cation channel that likely modulates the stability of neural circuits, particularly under environmental stresses. [Date last reviewed: 2019-02-28] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

DSC1, DSC, smi60E, Na-CP

Key Links

Genomic Location

Cytogenetic map

60E5-60E5

Sequence location

2R:24,891,614..24,921,677 [+]

Recombination map

2-107

RefSeq locus

NT_033778 REGION:24891614..24921677

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables voltage-gated monoatomic cation channel activity

inferred from direct assay

(Zhang et al., 2011)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables monoatomic cation channel activity

inferred from electronic annotation with InterPro:IPR043203

(InterPro Project Members, 2004-)

enables monoatomic ion channel activity

inferred from electronic annotation with InterPro:IPR005821

(InterPro Project Members, 2004-)

enables voltage-gated sodium channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000004137

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001696

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P04774

(Okamoto et al., 1987)

non-traceable author statement

(Littleton and Ganetzky, 2000)

Biological Process (7 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in olfactory behavior

inferred from mutant phenotype

(Kulkarni et al., 2002, Anholt et al., 1996)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

involved_in membrane depolarization during action potential

inferred from biological aspect of ancestor with PANTHER:PTN000004140

(GO Reference Genome Project, 2011-)

involved_in monoatomic ion transport

inferred from electronic annotation with InterPro:IPR005821

(InterPro Project Members, 2004-)

involved_in neuronal action potential

inferred from biological aspect of ancestor with PANTHER:PTN000798901

(GO Reference Genome Project, 2011-)

involved_in sodium ion transmembrane transport

inferred from sequence or structural similarity with UniProtKB:P04774

(Okamoto et al., 1987)

inferred from biological aspect of ancestor with PANTHER:PTN000798901

(GO Reference Genome Project, 2011-)

involved_in sodium ion transport

inferred from electronic annotation with InterPro:IPR001696

(InterPro Project Members, 2004-)

non-traceable author statement

(Littleton and Ganetzky, 2000)

involved_in transmembrane transport

inferred from electronic annotation with InterPro:IPR005821

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in membrane

inferred from electronic annotation with InterPro:IPR005821

(InterPro Project Members, 2004-)

located_in plasma membrane

inferred from electronic annotation with InterPro:IPR043203

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:P04774

(Okamoto et al., 1987)

non-traceable author statement

(Littleton and Ganetzky, 2000)

part_of voltage-gated sodium channel complex

inferred from electronic annotation with InterPro:IPR001696

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000004137

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

SODIUM CHANNEL-LIKE CATION CHANNEL SUBUNITS

Protein Family (UniProt)

Belongs to the sodium channel (TC 1.A.1.10) family. NaCP60E subfamily. (Q9W0Y8)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

SODIUM CHANNEL-LIKE CATION CHANNEL SUBUNITS -: The sodium-channel like cation channels are a family of ion channel proteins that contain a DEEA ion-selectivity pore motif. They have been found in a variety of invertebrate species. This family conducts several cations in a voltage-gated manner. (Adapted from FBrf0221058).

Protein Function (UniProtKB)

Mediates the voltage-dependent sodium ion permeability of excitable membranes. Plays a role in processing of olfactory information during the olfactory avoidance response.

(UniProt, Q9W0Y8)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

Na-CP: Na-channel-protein

Structural gene for sodium-channel protein.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\NaCP60E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W0Y8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216884

Gene model reviewed during 5.39

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

NaCP60E-RG

FBtr0308342

NM_166696

11631

2844

NaCP60E-RH

FBtr0308343

NM_001202078

11368

2844

NaCP60E-RI

FBtr0308344

NM_001259578

10469

2712

NaCP60E-RJ

FBtr0308345

NM_001259579

12929

2806

NaCP60E-RK

FBtr0308346

NM_001259580

11335

2833

NaCP60E-RL

FBtr0308347

NM_001259581

11512

2608

NaCP60E-RM

FBtr0330240

NM_001274243

11368

2896

NaCP60E-RN

FBtr0333815

NM_001274244

12573

934

NaCP60E-RO

FBtr0333816

NM_001274245

12475

366

NaCP60E-RP

FBtr0333817

NM_001274246

11475

2407

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

NaCP60E-PG

FBpp0300661

321.3

2844

6.92

FBpp0300662

321.3

2844

6.92

FBpp0300663

305.5

2712

6.82

FBpp0300664

316.6

2806

6.63

FBpp0300665

320.2

2833

6.95

FBpp0300666

296.8

2608

7.45

FBpp0303273

326.4

2896

7.10

FBpp0305949

106.6

934

6.58

FBpp0305950

42.9

366

6.34

FBpp0305951

273.8

2407

6.95

A0A0B4KF50

NP_001261175

AGB93705

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2844 aa isoforms: NaCP60E-PG, NaCP60E-PH

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Domain

The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1, S2, S3, S5, S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position.

(UniProt, Q9W0Y8)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\NaCP60E using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.66

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

neuropil of adult head

(Castella et al., 2001)

tract of adult head

(Castella et al., 2001)

first optic chiasma

(Castella et al., 2001)

second optic chiasma

(Castella et al., 2001)

giant fiber neuron

(Castella et al., 2001)

adult anterior optic tract

(Castella et al., 2001)

posterior optic commissure

(Castella et al., 2001)

medulla serpentine layer

(Castella et al., 2001)

adult gnathal ganglion

(Castella et al., 2001)

supraesophageal ganglion

(Castella et al., 2001)

adult ventral nerve cord

(Castella et al., 2001)

adult cervical connective

(Castella et al., 2001)

adult maxillary nerve

(Castella et al., 2001)

eye | faint

(Castella et al., 2001)

adult antennal nerve

(Castella et al., 2001)

adult great commissure

(Castella et al., 2001)

fan-shaped body | restricted

(Castella et al., 2001)

inferior ventral body commissure

(Castella et al., 2001)

superior ellipsoid commissure

(Castella et al., 2001)

adult medial antennal lobe tract | intense

(Castella et al., 2001)

adult anterior dorsal mesothoracic nerve

(Castella et al., 2001)

adult posterior dorsal mesothoracic nerve

(Castella et al., 2001)

adult dorsal metathoracic nerve

(Castella et al., 2001)

tract of mesothoracic segment

(Castella et al., 2001)

tract of metathoracic segment

(Castella et al., 2001)

labellum

(Castella et al., 2001)

mentum

(Castella et al., 2001)

adult labial nerve | restricted

(Castella et al., 2001)

nerve of femur

(Castella et al., 2001)

femoral campaniform sensillum

(Castella et al., 2001)

adult neuromuscular junction of femur

(Castella et al., 2001)

mesothoracic tibial trichoid sensillum

(Castella et al., 2001)

metathoracic tibial trichoid sensillum

(Castella et al., 2001)

prothoracic tibial trichoid sensillum

(Castella et al., 2001)

chaeta of cercus

(Castella et al., 2001)

adult stage | male

nerve of aedeagus

(Castella et al., 2001)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Castella et al., 2001)

Additional Descriptive Data

NaCP60E protein is expressed in the axons and neurites of many adult CNS and PNS neuropils, including the first and second optic chiasms, the anterior optic tract, giant fiber neurons of the lobula plate, posterior optic commmissure, the serpentine layer, subesophageal and supraesophageal and thoracic-abdominal ganglions, the cervical connective, maxillary, maxillary nerve, antennal nerve, great commissure, in part of the fan-shaped body, inferior ventral body commissure, inner antenno-cerebral tract, main nerve tracts of the meso- and metathoracic segments, the labellum and mentum of the proboscis, part of the labial nerve, nerve that innervates the femur, including the femoral sensilla and neuromuscular junction, sensilla of the tibia, bristles of the anal plate and the nerve that innervates the penis. It is also weakly expressed in the eye.

(Castella et al., 2001)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\NaCP60E in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\NaCP60E

View exonic expression by tissue for Dmel\NaCP60E

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0085434

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37981/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085434

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0085434

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085434

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0085434

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 34 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of NaCP60E

Transgenic constructs containing regulatory region of NaCP60E

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)M60E

(Stern et al., 1990)

Gene duplicated in

Dp(2;Y)bw⁺

(Stern et al., 1990)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

NaCP60E^GD2219

Other Phenotypes

Allele

abnormal behavior | recessive

NaCP60E^smi60E

abnormal smell perception

NaCP60E^smi60E

abnormal smell perception (with NaCP60E^EP590)

NaCP60E^smi60E

abnormal smell perception (with NaCP60E^EP1241)

NaCP60E^smi60E

abnormal smell perception (with NaCP60E^EP2250)

NaCP60E^smi60E

abnormal smell perception (with NaCP60E^EP2438)

NaCP60E^smi60E

abnormal smell perception (with NaCP60E^EP2555)

NaCP60E^smi60E

abnormal smell perception (with NaCP60E^smi60E)

chemical resistant

NaCP60E^DDT

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (27)

Hsap\SCN5A

5 of 14

Yes

Hsap\SCN10A

5 of 14

Yes

Hsap\SCN11A

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\CATSPER2

2 of 14

Yes

Hsap\CATSPER3

2 of 14

Yes

Hsap\CATSPER4

2 of 14

Yes

Hsap\NALCN

2 of 14

Hsap\TPCN1

2 of 14

Yes

Hsap\TPCN2

2 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (25)

Rnor\Scn5a

5 of 14

Yes

Rnor\Scn10a

5 of 14

Yes

Rnor\Scn11a

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Rnor\Catsper3

2 of 14

Yes

Rnor\Catsper4

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (27)

Mmus\Scn5a

5 of 14

Yes

Mmus\Scn10a

5 of 14

Yes

Mmus\Scn11a

5 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Mmus\Catsper2

2 of 14

Yes

Mmus\Catsper3

2 of 14

Yes

Mmus\Catsper4

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (21)

Xtro\scn1a

2 of 13

Yes

Xtro\scn3a

2 of 13

Yes

Xtro\scn5a

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (26)

Drer\scn1lab

4 of 14

Yes

Drer\scn5lab

4 of 14

Yes

Drer\scn8aa

4 of 14

Yes

Drer\scn12aa

4 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\cca-1

1 of 14

Yes

Cele\egl-19

1 of 14

Yes

Cele\F17C8.6

1 of 14

Yes

Cele\nca-2

1 of 14

Yes

Cele\unc-2

1 of 14

Yes

Cele\unc-77

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (9)

Agam\AgaP_AGAP007086

12 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (1)

Atha\TPC1

3 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\CCH1

6 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\cch1

4 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:NaCP60E. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (5)

para

8 of 13

Ca-α1T

6 of 13

cac

5 of 13

Ca-α1D

4 of 13

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 10 )

Human Ortholog

Disease

Evidence

References

SCN11A; sodium voltage-gated channel alpha subunit 11

model of familial episodic pain syndrome 3

IEA

(FlyBase, 2019-)

model of hereditary sensory and autonomic neuropathy type 7

IEA

(FlyBase, 2019-)

SCN10A; sodium voltage-gated channel alpha subunit 10

model of familial episodic pain syndrome 2

IEA

(FlyBase, 2019-)

SCN5A; sodium voltage-gated channel alpha subunit 5

model of long QT syndrome 3

IEA

(FlyBase, 2019-)

model of sudden infant death syndrome

IEA

(FlyBase, 2019-)

model of dilated cardiomyopathy 1E

IEA

(FlyBase, 2019-)

model of sick sinus syndrome

IEA

(FlyBase, 2019-)

model of Brugada syndrome 1

IEA

(FlyBase, 2019-)

model of familial atrial fibrillation

IEA

(FlyBase, 2019-)

model of progressive familial heart block type IA

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SCN5A; sodium voltage-gated channel alpha subunit 5

5 of 14

SODIUM VOLTAGE-GATED CHANNEL, ALPHA SUBUNIT 5; SCN5A

SCN10A; sodium voltage-gated channel alpha subunit 10

5 of 14

604427

EPISODIC PAIN SYNDROME, FAMILIAL, 2; FEPS2

familial episodic pain syndrome 2

SCN11A; sodium voltage-gated channel alpha subunit 11

5 of 14

604385

SCN2A; sodium voltage-gated channel alpha subunit 2

4 of 14

SODIUM VOLTAGE-GATED CHANNEL, ALPHA SUBUNIT 2; SCN2A

SCN3A; sodium voltage-gated channel alpha subunit 3

4 of 14

182391

SCN4A; sodium voltage-gated channel alpha subunit 4

4 of 14

603967

SCN8A; sodium voltage-gated channel alpha subunit 8

4 of 14

600702

SCN9A; sodium voltage-gated channel alpha subunit 9

4 of 14

603415

SCN1A; sodium voltage-gated channel alpha subunit 1

3 of 14

182389

SCN7A; sodium voltage-gated channel alpha subunit 7

3 of 14

182392

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

NaCP60E

enhanceable

smi61A

(Fedorowicz et al., 1998)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

smi61A

enhanceable

NaCP60E

(Fedorowicz et al., 1998)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63549

DroID - A comprehensive database of gene and protein interactions.

FBgn0085434

MIST (protein-protein) - An integrated Molecular Interaction Database

37981

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011, Zhang et al., 2011)

gene_with_stop_codon_read_through

(Jungreis et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-107

Cytogenetic map

60E5-60E5

Sequence location

2R:24,891,614..24,921,677 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60E5-60E5

Limits computationally determined from genome sequence between P{EP}CG9047^EP560&P{EP}CG3760^EP936 and P{EP}CG2790^EP412&P{EP}CG3776^EP835

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60E-60E

(determined by in situ hybridisation)

(Anholt et al., 1996, Ramaswami and Tanouye, 1989, Okamoto et al., 1987)

60D-60E

(determined by in situ hybridisation)

(Salkoff et al., 1987)

Experimentally Determined Recombination Data

Location

2-107

(FlyBase, 2016)

2-111.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (15)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

26012

y¹ v¹; P{TRiP.JF02037}attP2

85127

w¹¹¹⁸; PBac{RB}NaCP60E^e01582

95406

w¹¹¹⁸; PBac{PB}NaCP60E^c00635

37430

y¹ w^*; Mi{MIC}NaCP60E^MI04279

17133

w¹¹¹⁸; P{EP}NaCP60E^EP348 RpL41^EP348/CyO

60210

y¹ w^67c23; Mi{PT-GFSTF.0}NaCP60E^{MI04279-GFSTF.0}

Korea

14728

P{VT022112-lexA::GADfl}attP40

NIG-Fly

18506R-2

P{NIG.18506R}2

18506R-4

P{NIG.18506R}4

HMJ21453

y¹ v¹; P{TRiP.HMJ21453}attP40/CyO

VDRC

v101695

P{KK105476}VIE-260B

v106350

P{KK109809}VIE-260B

More stocks available...

Genomic Clones (28)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (52)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

MIP20602

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0085434

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0085434

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Castella et al., 2001)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Identified during a screen for olfactory mutants.

(Anholt et al., 1996)

The pattern of expression of NaCP60E throughout development has been analysed.

(Hong and Ganetzky, 1994)

NaCP60E RNA expression in pupae and adults has been studied.

(Tseng-Crank et al., 1991)

A partial NaCP60E cDNA has been cloned and sequenced.

(Ramaswami and Tanouye, 1989)

Isolated from a D.melanogaster genomic library using a probe corresponding to the transmembrane segment IVS6 of the eel sodium channel gene.

(Okamoto et al., 1987)

NaCP60E has been cloned and sequenced.

(Salkoff et al., 1987)

Identification: Isolated from a genomic library, using an eel sodium channel cDNA fragment as a probe.

(Salkoff et al., 1987)

Structural gene for sodium-channel protein.

Relationship to Other Genes

Source for database merge of

Source for merge of: NaCP60E CG18506

(FlyBase Genome Annotators, 2007)

Source for merge of: NaCP60E smi60E

(Kulkarni et al., 2002)

Additional comments

Annotations CG9071 and CG18506 merged as CG34405 in release 5.2 of the genome annotation.

(FlyBase Genome Annotators, 2007)

The P{lArB}NaCP60E^smi60E insertion (which results in a smell-impaired phenotype) maps within an intron of NaCP60E and is also near the promoter region of RpL41 (which is nested within the second intron of NaCP60E). Evidence indicates that the smell-impaired phenotype caused by the P{lArB}NaCP60E^smi60E insertion is due to an effect on NaCP60E and not RpL41; a revertant of NaCP60E^smi60E showing wild-type olfactory behaviour (NaCP60E^smi60E-rev16) shows full restoration of NaCP60E transcript expression and a massive reduction in RpL41 transcription.

(Kulkarni et al., 2002)

"NaCP60E" is a candidate gene for "smi60E".

(Anholt and Mackay, 2001)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

CG18506

(Accardo et al., 2004)

CG34405

(Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, FlyBase Genome Annotators, 2007)

CG9071

(Tsubouchi et al., 2012, Quinones-Coello, 2007, Molnar et al., 2006, Hoopengardner et al., 2003)

CT24831

(Kulkarni et al., 2002)

DIC60

(Okamoto et al., 1987)

DSC

(Stocker et al., 2013, Littleton and Ganetzky, 2000, Tseng-Crank et al., 1991, Ramaswami and Tanouye, 1989)

DSCI

(Hoopengardner et al., 2003)

Na-CP

(Lindsley and Zimm, 1992)

NaCP60E

(Corthals et al., 2023, Pang et al., 2021, Perry et al., 2020, Duan et al., 2018, Wang et al., 2018, Gene Disruption Project members, 2015-, Ashwal-Fluss et al., 2014, Hazelett et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012, Takács et al., 2012, Tsubouchi et al., 2012, Jungreis et al., 2011, Kuromi et al., 2010, Wasbrough et al., 2010, Quinones-Coello, 2007, Molnar et al., 2006, Harbison et al., 2005)

dsc

(Savva et al., 2012)

dsc1

(Kulkarni et al., 2002)

smi60E

(Mackay, 2004, Anholt and Mackay, 2001, Fedorowicz et al., 1998, Anholt et al., 1996)

Name Synonyms

CG18506

Na channel protein 60E

(Tsubouchi et al., 2012)

Na-channel-protein

(Lindsley and Zimm, 1992)

NaCP60E

(Harbison et al., 2005)

Sodium Channel 1

(Zhang et al., 2011)

put. sodium channel gene

(Salkoff, 1998.12.4)

smell impaired 60E

(Anholt et al., 1996)

Secondary FlyBase IDs

FBgn0016933
FBgn0002920
FBgn0035079

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 104 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37981

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W0Y8

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9W0Y8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W0Y8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0085434

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37981/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0085434

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0085434

FlyMine - An integrated database for Drosophila genomics

FBgn0085434

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG34405

MARRVEL_MODEL - MARRVEL (model organism gene)

37981

Linkouts

BioGRID - A database of protein and genetic interactions.

63549

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0085434

DroID - A comprehensive database of gene and protein interactions.

FBgn0085434

DRSC - Results frm RNAi screens

FBgn0085434

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0085434

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0085434

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

Flygut - An atlas of the Drosophila adult midgut

FBgn0085434

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008776

MIST (protein-protein) - An integrated Molecular Interaction Database

37981

References (104)

The following external sites may use different assemblies or annotations than FlyBase.

-0.66

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