Allele Dmel\dlp^Scer\UAS.cBa

General Information
Symbol	Dmel\dlp^Scer\UAS.cBa	Species	D. melanogaster
Name	Saccharomyces cerevisiae UAS construct a of Baeg	FlyBase ID	FBal0121805
Feature type	allele	Associated gene	Dmel\dlp

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02	Controlled Vocabulary Terms presynaptic active zone larval stage neuroanatomy defective NMJ bouton
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Baeg et al., 2001)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Scer\UAS regulatory sequences drive expression of full length dlp (an XhoI-XbaI fragment). (Baeg et al., 2001)
Carried in construct	P{UAS-dlp.WT} (Fox and Zinn, 2005, Johnson et al., 2004, Desbordes and Sanson, 2003, Baeg et al., 2001, Han et al., 2005, Baeg et al., 2004, Kirkpatrick et al., 2004, Avanesov et al., 2012, Yan et al., 2009, Yan et al., 2009, Yan et al., 2010, Kleinschmit et al., 2010, Johnson et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pharate adult stage, with Scer\GAL4^ptc-559.1 (Desbordes and Sanson, 2003) neuroanatomy defective \| larval stage, with Scer\GAL4^elav.PU (Johnson et al., 2006) neuroanatomy defective \| larval stage, with Scer\GAL4^how-24B (Johnson et al., 2006) neuroanatomy defective \| larval stage, with Scer\GAL4^how-24B, Scer\GAL4^sca.PU (Johnson et al., 2006) neuroanatomy defective \| larval stage, with Scer\GAL4^unspecified (Johnson et al., 2006) visible, with Scer\GAL4^bbg-C96 (Baeg et al., 2001) visible, with Scer\GAL4^en-e16E (Desbordes and Sanson, 2003)
Phenotype Manifest In
	NMJ bouton \| larval stage, with Scer\GAL4^elav.PU (Johnson et al., 2006) NMJ bouton \| larval stage, with Scer\GAL4^how-24B (Johnson et al., 2006) NMJ bouton \| larval stage, with Scer\GAL4^how-24B, Scer\GAL4^sca.PU (Johnson et al., 2006) presynaptic active zone \| larval stage, with Scer\GAL4^unspecified (Johnson et al., 2006) wing, with Scer\GAL4^en-e16E (Desbordes and Sanson, 2003) wing margin, with Scer\GAL4^bbg-C96 (Baeg et al., 2001) wing margin bristle, with Scer\GAL4^ap-md544, dlp¹ (Kirkpatrick et al., 2004) wing margin bristle, with Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) wing sensillum, with Scer\GAL4^bbg-C96 (Baeg et al., 2001)
Detailed Description
	Statement Reference Expression of dlp[Scer\UAS.cBa] under the control of Scer\GAL4[nub-AC-62] results in a very slight reduction in wing margin bristles compared to wild type. Wing blades are largely of normal size and show no signs of scalloping at the margin. (Avanesov et al., 2012) Expression of dlp[Scer\UAS.cBa] under the control of a postsynaptic Scer\GAL4 driver results in an increase in mean active zone area at the neuromuscular junction compared to wild type. Expression of dlp[Scer\UAS.cBa] under the control of either Scer\GAL4[how-24B] or Scer\GAL4[elav.PU] results in a reduction in bouton number at the neuromuscular junction compared to wild type. Expression of dlp[Scer\UAS.cBa] under the simultaneous control of both Scer\GAL4[sca.PU] and Scer\GAL4[how-24B] results in a reduction in bouton number at the neuromuscular junction compared to wild type. (Johnson et al., 2006) Expression of dlp^Scer\UAS.cBa, under the control of both Scer\GAL4^how-24B and Scer\GAL4^G14, does not produce a SNa phenotype. (Fox and Zinn, 2005) Expression of dlp^Scer\UAS.cBa, under the control of Scer\GAL4^ap-md544, in a dlp¹/+ background leads to bristle loss at the anterior wing margin. (Kirkpatrick et al., 2004) Adults expressing dlp^Scer\UAS.cBa under the control of Scer\GAL4^en-e16E have notched wings. (Desbordes and Sanson, 2003) Expression of dlp^Scer\UAS.cBa under the control of Scer\GAL4^C96 results in severe wing margin defects and loss of sensory bristles. (Baeg et al., 2001)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Scer\GAL4^bbg-C96, dlp^Scer\UAS.cBa has visible phenotype, suppressible by Scer\GAL4^bbg-C96/arm^{S10.Scer\UAS.T:Hsap\MYC} (Baeg et al., 2001) Scer\GAL4^bbg-C96, dlp^Scer\UAS.cBa has visible phenotype, suppressible by Scer\GAL4^bbg-C96/wg^{l-12.Scer\UAS} (Baeg et al., 2001)
Suppressor of
Statement Reference dlp^Scer\UAS.cBa/Scer\GAL4^elav.PLu is a suppressor \| partially of neuroanatomy defective phenotype of Sdc⁴⁸ (Johnson et al., 2004)
NOT Suppressor of
Statement Reference dlp^Scer\UAS.cBa/Scer\GAL4^how-24B is a non-suppressor of neuroanatomy defective \| larval stage phenotype of Sdc¹⁰⁶⁰⁸ (Johnson et al., 2006) dlp^Scer\UAS.cBa/Scer\GAL4^sli.PS is a non-suppressor of neuroanatomy defective phenotype of Sdc⁴⁸ (Johnson et al., 2004)
Phenotype Manifest In
Enhanced by
Statement Reference Scer\GAL4^nub-AC-62, dlp^Scer\UAS.cBa has wing margin bristle phenotype, enhanceable by Drer\wif1^{pVal.Scer\UAS.T:SV5\V5}, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) Scer\GAL4^nub-AC-62, dlp^Scer\UAS.cBa has wing margin bristle phenotype, enhanceable by Drer\wif1^{ΔEGF.Scer\UAS.T:SV5\V5}, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) Scer\GAL4^nub-AC-62, dlp^Scer\UAS.cBa has wing phenotype, enhanceable by Drer\wif1^{pVal.Scer\UAS.T:SV5\V5}, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012)
Suppressed by
Statement Reference Scer\GAL4^bbg-C96, dlp^Scer\UAS.cBa has wing margin phenotype, suppressible by Scer\GAL4^bbg-C96/arm^{S10.Scer\UAS.T:Hsap\MYC} (Baeg et al., 2001) Scer\GAL4^bbg-C96, dlp^Scer\UAS.cBa has wing margin phenotype, suppressible by Scer\GAL4^bbg-C96/wg^{l-12.Scer\UAS} (Baeg et al., 2001) Scer\GAL4^bbg-C96, dlp^Scer\UAS.cBa has wing sensillum phenotype, suppressible by Scer\GAL4^bbg-C96/arm^{S10.Scer\UAS.T:Hsap\MYC} (Baeg et al., 2001) Scer\GAL4^bbg-C96, dlp^Scer\UAS.cBa has wing sensillum phenotype, suppressible by Scer\GAL4^bbg-C96/wg^{l-12.Scer\UAS} (Baeg et al., 2001)
Enhancer of
Statement Reference dlp^Scer\UAS.cBa, Scer\GAL4^nub-AC-62 is an enhancer of wing margin bristle phenotype of Drer\wif1^{pVal.Scer\UAS.T:SV5\V5}, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) dlp^Scer\UAS.cBa, Scer\GAL4^nub-AC-62 is an enhancer of wing margin bristle phenotype of Drer\wif1^Scer\UAS.cAa, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) dlp^Scer\UAS.cBa, Scer\GAL4^nub-AC-62 is an enhancer of wing margin bristle phenotype of Drer\wif1^{ΔEGF.Scer\UAS.T:SV5\V5}, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) dlp^Scer\UAS.cBa, Scer\GAL4^nub-AC-62 is an enhancer of wing phenotype of Drer\wif1^{pVal.Scer\UAS.T:SV5\V5}, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) dlp^Scer\UAS.cBa, Scer\GAL4^nub-AC-62 is an enhancer of wing phenotype of Drer\wif1^Scer\UAS.cAa, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) dlp^Scer\UAS.cBa/Scer\GAL4^nub-AC-62 is an enhancer of wing margin bristle phenotype of Scer\GAL4^nub-AC-62, shf::Drer\wif1^{EDF-WIF.Scer\UAS.T:SV5\V5} (Avanesov et al., 2012) dlp^Scer\UAS.cBa/Scer\GAL4^nub-AC-62 is an enhancer of wing phenotype of shf::Drer\wif1^{EDF-WIF.Scer\UAS.T:SV5\V5} (Avanesov et al., 2012)
NOT Enhancer of
Statement Reference dlp^Scer\UAS.cBa, Scer\GAL4^nub-AC-62 is a non-enhancer of wing phenotype of Drer\wif1^{ΔEGF.Scer\UAS.T:SV5\V5}, Scer\GAL4^nub-AC-62 (Avanesov et al., 2012) dlp^Scer\UAS.cBa/Scer\GAL4^nub-AC-62 is a non-enhancer of wing phenotype of Scer\GAL4^nub-AC-62, fz2^{GPI.Scer\UAS.T:Hsap\MYC} (Avanesov et al., 2012)
Suppressor of
Statement Reference dlp^Scer\UAS.cBa/Scer\GAL4^elav.PLu is a suppressor \| partially of presumptive embryonic/larval central nervous system phenotype of Sdc⁴⁸ (Johnson et al., 2004)
NOT Suppressor of
Statement Reference dlp^Scer\UAS.cBa/Scer\GAL4^how-24B is a non-suppressor of NMJ bouton \| larval stage phenotype of Sdc¹⁰⁶⁰⁸ (Johnson et al., 2006) dlp^Scer\UAS.cBa/Scer\GAL4^sli.PS is a non-suppressor of presumptive embryonic/larval central nervous system phenotype of Sdc⁴⁸ (Johnson et al., 2004)
Other
Statement Reference Scer\GAL4^bbg-C96, arm^{S10.Scer\UAS.T:Hsap\MYC}, dlp^Scer\UAS.cBa has wing margin bristle \| ectopic phenotype (Baeg et al., 2001, Baeg et al., 2001) Scer\GAL4^bbg-C96, dlp^Scer\UAS.cBa, wg^{l-12.Scer\UAS} has wing margin bristle \| ectopic phenotype (Baeg et al., 2001, Baeg et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Expression of dlp[Scer\UAS.cBa] does not enhance the reduction in adult wing size seen when fz2[GPI.Scer\UAS.T:Hsap\MYC] is expressed under the control of Scer\GAL4[nub-AC-62]. (Avanesov et al., 2012) Expression of dlp[Scer\UAS.cBa] under the control of Scer\GAL4[how-24B] does not rescue the reduction in bouton number at the neuromuscular junction seen in homozygous Sdc[10608] larvae. Larvae co-expressing Sdc[Scer\UAS.cJa] and dlp[Scer\UAS.cBa] under the control of Scer\GAL4[how-24B] show a reduction in bouton number at the neuromuscular junction compared to wild type, as occurs in larvae expressing dlp[Scer\UAS.cBa] alone under the control of Scer\GAL4[how-24B]. (Johnson et al., 2006) Expression of dlp^Scer\UAS.cBa under the control of Scer\GAL4^sli.PS fails to rescue the axon midline crossing phenotype seen in Df(2R)48 embryos in which Sara function has been rescued by Sara^Ubi.PJ. Expression of dlp^Scer\UAS.cBa under the control of Scer\GAL4^elav.PLu significantly rescues the axon midline crossing phenotype seen in Df(2R)48 embryos in which Sara function has been rescued by Sara^Ubi.PJ. (Johnson et al., 2004) Co-expression of wg^{l-12.Scer\UAS} or arm^{S10.Scer\UAS.T:Hsap\MYC} can rescue both the wing notching phenotype and loss of sensory bristles caused by expression of dlp^Scer\UAS.cBa under the control of Scer\GAL4^C96. In addition, ectopic margin bristles close to the wing margin are seen in these flies. (Baeg et al., 2001)
Xenogenetic Interactions
Statement Reference Expression of dlp[Scer\UAS.cBa] strongly enhances the wing scalloping and bristle loss phenotypes seen when Drer\wif1[Scer\UAS.cAa] is expressed under the control of Scer\GAL4[nub-AC-62]. Expression of dlp[Scer\UAS.cBa] synergistically enhances the wing margin scalloping and wing blade reduction seen when Drer\wif1[pVal.Scer\UAS.T:SV5\V5] is expressed under the control of Scer\GAL4[nub-AC-62]. The loss of wing margin bristles is also enhanced, consistent with the two transgenes having an additive effect. dlp[Scer\UAS.cBa] additively enhances the loss of wing margin bristles seen when Drer\wif1[ΔEGF.Scer\UAS.T:SV5\V5] is expressed under the control of Scer\GAL4[nub-AC-62]. The wing margin defects seen in Drer\wif1[ΔEGF.Scer\UAS.T:SV5\V5] are not enhanced by dlp[Scer\UAS.cBa]. Expression of dlp[Scer\UAS.cBa] synergistically enhances the wing scalloping and bristle loss phenotypes seen when shf::Drer\wif1[EDF-WIF.Scer\UAS.T:SV5\V5] is expressed under the control of Scer\GAL4[nub-AC-62]. Wing margin bristles and the first longitudinal vein are almost completely eliminated and wing size is further reduced. (Avanesov et al., 2012)
Complementation & Rescue Data
Rescues	dlp^Scer\UAS.cBa/Scer\GAL4^ap-md544 rescues dlp¹/Df(3L)fz-D21 (Kirkpatrick et al., 2004) dlp^Scer\UAS.cBa/Scer\GAL4^ap-md544 rescues dlp¹/dlp² (Kirkpatrick et al., 2004) Scer\GAL4^prd.RG1/dlp^Scer\UAS.cBa rescues dlp^A187 (Yan et al., 2010)
Partially rescues	dlp^Scer\UAS.cBa/Scer\GAL4[-] partially rescues dlp¹/Df(3L)fz-D21 (Kirkpatrick et al., 2004)
Comments	Expression of dlp[Scer\UAS.cBa] under the control of Scer\GAL4[prd.RG1] restores cuticle patterning in entire segments in a non-cell-autonomous manner in dlp[A187] embryos derived from dlp[A187] female germline clones. (Yan et al., 2010) Expression of dlp^Scer\UAS.cBa without a driver is sufficient to rescue the eye phenotype, but not the wing phenotype of dlp¹/Df(3L)fz-D21 mutants. (Kirkpatrick et al., 2004)
Stocks ( 1 )
Bloomington	9160 w¹¹¹⁸; P{UAS-dlp.WT}3
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	dlp^Scer\UAS.cBa
Name Synonym	Saccharomyces cerevisiae UAS construct a of Baeg (Baeg et al., 2001)
Secondary FlyBase IDs

References ( 13 )
Research paper	Avanesov et al., 2012, PLoS Genet. 8(2): e1002503 The Role of Glypicans in Wnt Inhibitory Factor-1 Activity and the Structural Basis of Wif1's Effects on Wnt and Hedgehog Signaling. [FBrf0217590] Kleinschmit et al., 2010, Dev. Biol. 345(2): 204--214 Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation. [FBrf0211640] Yan et al., 2010, Development 137(12): 2033--2044 The cell-surface proteins Dally-like and Ihog differentially regulate Hedgehog signaling strength and range during development. [FBrf0210906] Yan et al., 2009, Dev. Cell 17(4): 470--481 The core protein of glypican Dally-like determines its biphasic activity in wingless morphogen signaling. [FBrf0209080] Johnson et al., 2006, Neuron 49(4): 517--531 The HSPGs Syndecan and Dallylike bind the receptor phosphatase LAR and exert distinct effects on synaptic development. [FBrf0191320] Han et al., 2005, Development 132(4): 667--679 Drosophila glypicans Dally and Dally-like shape the extracellular Wingless morphogen gradient in the wing disc. [FBrf0183936] Baeg et al., 2004, Dev. Biol. 276(1): 89--100 The Wingless morphogen gradient is established by the cooperative action of Frizzled and Heparan Sulfate Proteoglycan receptors. [FBrf0182539] Johnson et al., 2004, Curr. Biol. 14(6): 499--504 Axonal heparan sulfate proteoglycans regulate the distribution and efficiency of the repellent slit during midline axon guidance. [FBrf0174485] Kirkpatrick et al., 2004, Dev. Cell 7(4): 513--523 Spatial regulation of Wingless morphogen distribution and signaling by Dally-like protein. [FBrf0180126] Desbordes and Sanson, 2003, Development 130(25): 6245--6255 The glypican Dally-like is required for Hedgehog signalling in the embryonic epidermis of Drosophila. [FBrf0167512] Baeg et al., 2001, Development 128(1): 87--94 Heparan sulfate proteoglycans are critical for the organization of the extracellular distribution of Wingless. [FBrf0131247]
Supplementary material	Yan et al., 2009, Dev. Cell 17(4): Supplemental Data. [FBrf0211306] Fox and Zinn, 2005, Curr. Biol. 15(19): Supplemental data. [FBrf0191745]

Allele Dmel\dlpScer\UAS.cBa

Allele Dmel\dlp^Scer\UAS.cBa