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Allele Dmel\kcc^DHS1

General Information
Symbol	Dmel\kccDHS1	Species	D. melanogaster
Name		FlyBase ID	FBal0144994
Feature type	allele	Associated gene	Dmel\kcc
Allele class
Mutagen	Delta2-3
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	Delta2-3 (Zhang et al., 2002)
Mutations Mapped to the Genome
Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Progenitor genotype
Nature of the lesion	Statement Reference An insertion of 13bp (ACTATGCTACTGT) after the 7th base pair of the intron 11. (Hekmat-Scafe et al., 2006)
Caused by insertion	P{lacZ}J (Zhang et al., 2002)
Cytology
Phenotypic Data
Phenotypic Class
	bang sensitive (Zhang et al., 2002) bang sensitive (with kccAd-4) (Hekmat-Scafe et al., 2006) bang sensitive (with kccEY08304) (Hekmat-Scafe et al., 2006) bang sensitive (with kccML1) (Hekmat-Scafe et al., 2006) bang sensitive (with kccP20-180) (Hekmat-Scafe et al., 2006) bang sensitive | recessive (Hekmat-Scafe et al., 2006) neurophysiology defective | recessive (Hekmat-Scafe et al., 2006) paralytic | recessive (Hekmat-Scafe et al., 2010)
Phenotype Manifest In
Detailed Description
	Statement Reference Homozygous kcc[DHS1] mutants display a bang-sensitive paralytic phenotype. (Hekmat-Scafe et al., 2010) One day after eclosion, 38 % of kcc[DHS1] homozygous flies are bang sensitive (i.e.- they exhibit temporary paralysis in response to 10 seconds of vortexing). The penetrance of bang sensitivity in these flies decreases rapidly with age. The same phenotype is seen in kcc[DHS1]/kcc[EY08304] animals, and at a lower penetrance (12-13%) in kcc[DHS1]/kcc[ML1] or kcc[DHS1]/kcc[P20-180] animals. The seizure threshold of kcc[DHS1] homozygotes (the voltage of high frequency stimulation to the brain required to induce seizures) is significantly lower than wild-type. (Hekmat-Scafe et al., 2006) 60% of homozygotes have a bang sensitive phenotype. (Zhang et al., 2002)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference kccDHS1 has bang sensitive | recessive phenotype, enhanceable by bss[+]/parabss1 (Hekmat-Scafe et al., 2006) kccDHS1 has bang sensitive | recessive phenotype, enhanceable by eas[+]/eas2 (Hekmat-Scafe et al., 2006) kccDHS1 has bang sensitive | recessive phenotype, enhanceable by jbugunspecified (Hekmat-Scafe et al., 2006) kccDHS1 has bang sensitive | recessive phenotype, enhanceable by sdaiso7.8/sda[+] (Hekmat-Scafe et al., 2006)
Suppressed by
Statement Reference kccDHS1 has bang sensitive | recessive phenotype, suppressible by Rdl[+]/Rdl1 (Hekmat-Scafe et al., 2006) kccDHS1 has paralytic | recessive phenotype, suppressible | partially by Df(3L)eygC1/+ (Hekmat-Scafe et al., 2010) kccDHS1 has paralytic | recessive phenotype, suppressible | partially by Hsap\SLC12A5Scer\UAS.cHa/Scer\GAL4c739 (Hekmat-Scafe et al., 2010) kccDHS1 has paralytic | recessive phenotype, suppressible | partially by Ncc69PL00618/Ncc69[+] (Hekmat-Scafe et al., 2010) kccDHS1 has paralytic | recessive phenotype, suppressible | partially by Rdl[+]/Rdl1 (Hekmat-Scafe et al., 2010) kccDHS1 has paralytic | recessive phenotype, suppressible | partially by Rdl[+]/RdlMD-RR (Hekmat-Scafe et al., 2010) kccDHS1 has paralytic | recessive phenotype, suppressible | partially by Scer\GAL4c747/Ncc69GD14863 (Hekmat-Scafe et al., 2010) kccDHS1 has paralytic | recessive phenotype, suppressible | partially by Scer\GAL4c747/RdldsRNA.8-10.Scer\UAS (Hekmat-Scafe et al., 2010) kccDHS1 has paralytic | recessive phenotype, suppressible | partially by Scer\GAL4Mef2.247/Scer\GAL4Mef2.247/Ctet\TeTxLCScer\UAS.cTa (Hekmat-Scafe et al., 2010)
NOT suppressed by
Statement Reference kccDHS1 has paralytic | recessive phenotype, non-suppressible by Scer\GAL4GH146/Ctet\TeTxLCScer\UAS.cTa (Hekmat-Scafe et al., 2010)
Enhancer of
Statement Reference kccDHS1/E(sda)J[+] is an enhancer of bang sensitive phenotype of sdaiso7.8 (Zhang et al., 2002)
Other
Statement Reference bss[+]/parabss1, kccDHS1 has bang sensitive | dominant phenotype (Hekmat-Scafe et al., 2006) kccDHS1, sdaiso7.8/sda[+] has bang sensitive | dominant phenotype (Hekmat-Scafe et al., 2006)
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants is significantly suppressed by Rdl[1]/+ or Rdl[MD-RR]/+. The bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants is suppressed to great extent by Scer\GAL4[c747]-driven Rdl[dsRNA.8-10.Scer\UAS] expression. The bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants is only modestly suppressed by Ncc69[PL00618]/+ or Df(3L)eyg[C1]/+. The bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants is markedly suppressed by the expression of Ncc69[GD14863] via Scer\GAL4[c747]. (Hekmat-Scafe et al., 2010) The penetrance of the bang sensitive phenotype due to kcc[DHS1]/kcc[DHS1] is enhanced by jbug[unspecified]. Unlike either heterozygote alone, a bang sensitive phenotype is seen in kcc[DHS1]/+; sda[iso7.8]/+ adults (penetrance 51%). The penetrance of this phenotype rises to 82% in kcc[DHS1]/kcc[DHS1]; sda[iso7.8]/+ adults. I nearly identical pattern of enhancement is seen with bss[1]: 48% of kcc[DHS1]/+; bss[1]/+ adults are bang sensitive, rising to 77% in kcc[DHS1]/kcc[DHS1]/; bss[1]/+ adults. No bang sensitivity is seen in kcc[DHS1]/+; eas[2]/+ transheterozygotes, but 65% of kcc[DHS1]/kcc[DHS1] ; eas[2]/+ adults are bang sensitive. The penetrance of the bang sensitive phenotype seen in kcc[DHS1] homozygous adults is reduced to 1% by Rdl[1]/+ and to 3% by Gad1[L352F]/+. (Hekmat-Scafe et al., 2006)
Xenogenetic Interactions
Statement Reference Expression of two copies of Hsap\SLC12A5[Scer\UAS.cHa] driven by two copies of Scer\GAL4[c739] results in an almost complete rescue of the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Electrophysiology shows that Hsap\SLC12A5[Scer\UAS.cHa] expression raises the seizure threshold of kcc[DHS1] mutant flies. Blocking synaptic transmission from mushroom body neurons by Scer\GAL4[Mef2.247]-directed expression of Ctet\TeTxLC[Scer\UAS.cTa] dramatically reduces the bang sensitivity of kcc[DHS1] mutant flies. Blocking synaptic transmission by Ctet\TeTxLC[Scer\UAS.cTa] expression in antennal neurons projecting to the mushroom body (targeted by Scer\GAL4[GH146]) produces no significant difference in the bang sensitivity of kcc[DHS1] mutant flies. (Hekmat-Scafe et al., 2010)
Complementation & Rescue Data
Partially rescued by	kccDHS1 is partially rescued by kccScer\UAS.cHb/Scer\GAL41407 (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by kccScer\UAS.cHb/Scer\GAL4c739 (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by kccScer\UAS.cHb/Scer\GAL4Cha.7.4 (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by kccScer\UAS.cHb/Scer\GAL4elav.PLu (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by kccScer\UAS.cHb/Scer\GAL4GH146 (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by kccScer\UAS.cHd/Scer\GAL41407 (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by kccScer\UAS.cHd/Scer\GAL4elav.PLu (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by kccScer\UAS.cHd/Scer\GAL4repo.PU (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL4104Y/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL4Aph-4-c232/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL4c305a/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL4c747/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL4ey-OK107/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL4Gad1.3.098/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL4Mef2.247/Scer\GAL4Mef2.247/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL4Rdl.PS/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL4Tab2-201Y/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010) kccDHS1 is partially rescued by Scer\GAL80Mef2.PT/kccScer\UAS.cHb/Scer\GAL4elav.PLu (Hekmat-Scafe et al., 2010)
Not rescued by	kccDHS1 is not rescued by Scer\GAL4repo.PU/kccScer\UAS.cHb (Hekmat-Scafe et al., 2010)
Comments	Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[repo.PU] produces no apparent change in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[elav.PLu] results in a substantial reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[1407] results in a substantial reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHd] driven by Scer\GAL4[repo.PU] produces a slight reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHd] driven by Scer\GAL4[elav.PLu] results in a modest reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHd] driven by Scer\GAL4[1407] results in a modest reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[ey-OK107] results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[Aph-4-c232] results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[104Y] results only in a modest reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[GH146] results in a substantial reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Co-expression of Scer\GAL80[Mef2.PT] with Scer\GAL4[elav.PLu]-driven kcc[Scer\UAS.cHb] results in a significantly compromise in the rescuing ability of kcc[Scer\UAS.cHb]. Scer\GAL80[Mef2.PT] co-expression significantly compromises the ability of Scer\GAL4[elav.PLu]-driven kcc[Scer\UAS.cHb] to rescue the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[Mef2.247] results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[c747] results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[c739] results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[c305a] results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[Tab2-201Y] results in a marked reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[Rdl.PS] results in a substantial reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[Gad1.3.098] results in a significant reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. Expression of kcc[Scer\UAS.cHb] driven by Scer\GAL4[Cha.7.4] results only in a modest reduction in the bang-sensitive paralytic phenotype of homozygous kcc[DHS1] mutants. (Hekmat-Scafe et al., 2010)
Stocks ( 0 )
Notes on Origin
Discoverer
	Selected as: a mutation that dominantly enhances the sdaiso7.8/+ bang sensitive phenotype. (Zhang et al., 2002)
Comments
	Splicing is unaffected by this mutation, but transcript levels are significantly reduced. Protein levels are low immediately after birth, but recover significantly with age. (Hekmat-Scafe et al., 2006)
External Crossreferences & Linkouts
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	E(sda)JJ   kccDHS1 (Hekmat-Scafe et al., 2010) Line J (Zhang et al., 2002)
Name Synonym
Secondary FlyBase IDs
References ( 3 )
Research paper	Hekmat-Scafe et al., 2010, Genetics 184(1): 171--183 Seizure sensitivity is ameliorated by targeted expression of k+-cl- cotransporter function in the mushroom body of the Drosophila brain. [FBrf0209683] Hekmat-Scafe et al., 2006, J. Neurosci. 26(35): 8943--8954 Mutations in the K+/Cl- cotransporter gene kazachoc (kcc) increase seizure susceptibility in Drosophila. [FBrf0192588] Zhang et al., 2002, Genetics 162(3): 1283--1299 The Drosophila slamdance gene: a mutation in an aminopeptidase can cause seizure, paralysis and neuronal failure. [FBrf0155803]