FlyBase Allele Report: Dmel\aub[HN2]

General Information

Symbol

Dmel\aub^HN2

Species

D. melanogaster

Name

FlyBase ID

FBal0000798

Feature type

allele

Associated gene

Dmel\aub

Associated Insertion(s)

Carried in Construct

Also Known As

aub^HN

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schupbach and Wieschaus, 1991)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q622term.

(Harris and Macdonald, 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,998,669..10,998,669 [-]

Nucleotide change:

C10998669T

Amino acid change:

Q622term | aub-PA; Q551term | aub-PC

Reported amino acid change:

Q622term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Harris and Macdonald, 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates amyotrophic lateral sclerosis type 6

modeled by caz^RNAi.UAS.cUa

(Wakisaka et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | female (with aub^N11)

(Pek and Kai, 2011)

decreased cell number | progressive | spermatogenesis (with aub^QC42)

(Adashev et al., 2024)

female semi-fertile (with aub^QC42)

(Atikukke et al., 2014)

female sterile

(Chen et al., 2007, Schupbach and Wieschaus, 1991)

lethal | maternal effect | recessive

(Wilson et al., 1996)

lethal - all die during embryonic stage | maternal effect (with aub^QC42)

(Barckmann et al., 2015, Orsi et al., 2010)

Phenotype Manifest In

cystoblast (with aub^N11)

(Pek and Kai, 2011)

dorsal appendage (with aub^QC42)

(Orsi et al., 2010, Klattenhoff et al., 2007)

dorsal appendage | maternal effect (with aub^QC42)

(Barckmann et al., 2015)

egg chorion (with aub^QC42)

(Chen et al., 2007)

embryo | maternal effect | posterior

(Wilson et al., 1996)

embryonic/larval cuticle | embryonic stage | maternal effect (with aub^N11)

(Rouget et al., 2010)

female germline stem cell (with aub^N11)

(Pek and Kai, 2011)

male germline stem cell | decreased number (with aub^QC42)

(Adashev et al., 2024)

spermatocyte | spermatogenesis (with aub^QC42)

(Bozzetti et al., 2015)

spermatocyte | spermatogenesis (with aub^sting-1)

(Bozzetti et al., 2015)

Detailed Description

Statement

Reference

Embryos from aub^HN2/aub^QC42 mothers are completely lethal and frequently display fusion of dorsal appendages.

(Barckmann et al., 2015)

aub^HN2/aub^QC42 and aub^HN2/aub^sting-1 transheterozygotes exhibit crystalline aggregates (of Stellate protein) in spermatocytes, which are absent in aub^HN2 single heterozygotes and wild-type controls; aub^HN2/aub^QC42 transheterozygotes do not display significant changes in the length of third instar larval neuromuscular junctions, as compared to controls.

(Bozzetti et al., 2015)

aub^HN2/aub^QC42 mutant females exhibit a modest decrease in egg laying capacity (75% of wild type). Very few compound egg chambers (multiple cysts in a single follicular epithelium) are seen.

(Atikukke et al., 2014)

Newly eclosed aub^HN2/aub^QC42 transheterozygous females do not show any obvious changes in the number of germ line stem cells in the germarium, as compared to controls.

(Ma et al., 2014)

Germline stem cells and cystoblasts of aub^N11/aub^HN ovaries show delays in prometaphase and chromosome segregation defects.

(Pek and Kai, 2011)

Eggs laid by aub^HN2/aub^QC42 females have dorsal appendage defects; 52.4% have fused dorsal appendages, 24.5% lack dorsal appendages and 23.1% have wild-type dorsal appendages.

(Orsi et al., 2010)

No embryos derived from aub^N11/aub^HN2 females produce a cuticle.

(Rouget et al., 2010)

Eggs laid by aub^QC42/aub^HN females show a variable ventralised phenotype.

(Chen et al., 2007)

aub^HN/aub^QC42 eggs show fused dorsal appendages in 40% of cases, no appendages in 12% of cases and the wild-type number of appendages in 48% of cases.

(Klattenhoff et al., 2007)

Perinuclear nuage appears smoother than normal in aub^HN/aub^QC42 nurse cells, but nuage particles are unaffected.

(Snee and Macdonald, 2004)

2% of eggs from homozygous mothers are fertilized and embryos lack abdominal segments and pole cells, i.e. show a classic posterior group mutant phenotype.

(Wilson et al., 1996)

Most extreme eggs are spindle shaped without dorsal appendages. Other eggs have fused dorsal appendages or of normal morphology but remain unfertilized.

(Schupbach and Wieschaus, 1991)

female-sterile

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(3L)Exel6095/CycJ^null, armi^+t6.3, aub^HN2/aub^QC42 has female sterile phenotype, suppressible | partially by CycJ^+t4

(Atikukke et al., 2014)

Enhancer of

Statement

Reference

aub[+]/aub^HN2 is an enhancer of abnormal meiotic cell cycle phenotype of RanGAP^Sd

(Gell and Reenan, 2013)

NOT Enhancer of

Statement

Reference

aub[+]/aub^HN2 is a non-enhancer of partially lethal - majority die | male phenotype of Df(1)roX2Δ, lncRNA:roX1^ex33A

(Menon and Meller, 2012)

Suppressor of

Statement

Reference

aub[+]/aub^HN2 is a suppressor | partially of partially lethal - majority live phenotype of ct^A, lncRNA:flam^A

(Guida et al., 2016)

NOT Suppressor of

Statement

Reference

aub^HN2/aub^QC42 is a non-suppressor of increased cell death | oogenesis phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

aub^HN2/aub^QC42 is a non-suppressor of increased cell number | oogenesis phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

aub[+]/aub^HN2 is a non-suppressor of partially lethal - majority die | male phenotype of Df(1)roX2Δ, lncRNA:roX1^ex33A

(Menon and Meller, 2012)

aub^HN2/aub^N11 is a non-suppressor of abnormal eye color phenotype of w^RNAi.GMR

(Pelisson et al., 2007)

Other

Statement

Reference

Df(3L)Exel6095/CycJ^null, armi^+t6.3, aub^HN2/aub^QC42 has female sterile phenotype

(Atikukke et al., 2014)

+/SD-72, aub^HN2 has lethal | dominant phenotype

(Gell and Reenan, 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Df(3L)Exel6095/CycJ^null, armi^+t6.3, aub^HN2/aub^QC42 has egg chamber phenotype, suppressible by CycJ^+t4

(Atikukke et al., 2014)

aub^HN2/aub^QC42 has egg chorion phenotype, suppressible by lok^unspecified

(Chen et al., 2007)

aub^HN2/aub^QC42 has dorsal appendage phenotype, suppressible by mei-41^D3/mei-41^D3

(Klattenhoff et al., 2007)

Enhancer of

Statement

Reference

aub[+]/aub^HN2 is an enhancer of abdominal segment phenotype of nanos^+1+3.Tag:MS2, nanos^BN

(Becalska et al., 2011)

Suppressor of

Statement

Reference

aub^HN2/aub^N11 is a suppressor of primordial germ cell | ectopic phenotype of osk^short.bcd

(Harris and Macdonald, 2001)

aub^HN2 is a suppressor of phenotype of osk^{bcd.3UTR.Tag:polyHis}

(Wilson et al., 1996, Wilson et al., 1996)

NOT Suppressor of

Statement

Reference

aub^HN2/aub^QC42 is a non-suppressor of ovary phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

aub^HN2/aub^QC42 is a non-suppressor of ovariole phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

aub^HN2/aub^QC42 is a non-suppressor of egg chamber phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

aub^HN2/aub^QC42 is a non-suppressor of female germline cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

aub^HN2/aub^QC42 is a non-suppressor of nurse cell | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

aub^HN2/aub^QC42 is a non-suppressor of oocyte | increased number phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

aub^HN2/aub^QC42 is a non-suppressor of follicle cell phenotype of l(3)mbt^GM76/Df(3R)ED10966

(Coux et al., 2018)

aub[+]/aub^HN2 is a non-suppressor of spermatocyte | spermatogenesis phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

aub^UAS.cBa, aub[+], aub^HN2, Scer\GAL4^upd1.PU is a non-suppressor of spermatocyte | spermatogenesis phenotype of Fmr1^Δ113M

(Bozzetti et al., 2015)

aub^HN2/aub^N11 is a non-suppressor of eye phenotype of w^RNAi.GMR

(Pelisson et al., 2007)

Other

Statement

Reference

Df(3L)Exel6095/CycJ^null, armi^+t6.3, aub^HN2/aub^QC42 has egg chamber phenotype

(Atikukke et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

aub^HN2 heterozygosity suppresses the mortality exhibited by flam^A, ct^A double homozygotes.

(Guida et al., 2016)

Neither aub^HN2 heterozygosity nor the combination of aub^HN2 heterozygosity and expression of aub^Scer\UAS.cBa under the control of Scer\GAL4^upd1.PU are able to suppress the crystalline aggregates (of Stellate protein) observed in the spermatocytes of Fmr1^Δ113M heterozygotes.

AGO1^k08121, aub^HN2 double homozygotes do not display significant changes in the length of third instar larval neuromuscular junctions, as compared to controls.

(Bozzetti et al., 2015)

CycJ^null/Df(3L)Exel6095 (expressing armi^+t6.3 to restore armi[+] function) enhances the fertility defects seen in aub^HN2/aub^QC42 mutant females. The resulting flies lay no eggs and oocyte development is arrested, with egg chambers rarely advancing beyond stage 7-8. Compound egg chambers (multiple cysts in a single follicular epithelium) are seen, and these often include a disorganised layer of follicle cells almost separating two fully developed cysts. This phenotype can be fully suppressed by expression of CycJ^+t4.

(Atikukke et al., 2014)

The segregation distortion seen in males carrying either the SD-5star or SD-LosArenos Dp(2;2)RanGAP^SD chromosome variant (in a sensitive Rsp background) is enhanced (the k[[c]] value, or proportion of Dp(2;2)RanGAP^SD-bearing progeny as the fraction of total progeny (corrected for viability), is significantly increased) if the males are also carrying a single copy of aub^HN2.

(Gell and Reenan, 2013)

The survival of roX1^ex33A Df(1)roX2Δ adult males is not significantly altered if they are also heterozygous for aub^HN2.

(Menon and Meller, 2012)

nos^BN,nos^{+1+3.T:MS2\MCP.BS}/nos^BN embryos carrying a aub^HN/+ mutation display a reduction in abdominal segmentation, forming an average of 3-4 abdominal segments.

(Becalska et al., 2011)

The dorsal appendage defects seen in eggs laid by aub^HN2/aub^QC42 females are suppressed if the females are also homozygous for lok^p6; 99.2% of the eggs have wild-type dorsal appendages.

(Orsi et al., 2010)

The ventralised phenotype seen in eggs derived from aub^QC42/aub^HN females is partially suppressed by lok^unspecified.

(Chen et al., 2007)

The fused dorsal appendage phenotype of aub^HN/aub^QC42 eggs is suppressed in lok^p6; aub^HN/aub^QC42 eggs with 98% of double mutants showing wild-type appendage morphology.

The mei-41^D3 mutation suppresses the fused dorsal appendage phenotype of aub^HN/aub^QC42 eggs; 85% of mei-41^D3; aub^HN/aub^QC42 eggs show wild-type appendage morphology.

(Klattenhoff et al., 2007)

The yellowish eye colour caused by expression of one copy of w^dsRNA.GMR in a wild-type background is not altered if the flies are also carrying aub^HN2/aub^N11.

(Pelisson et al., 2007)

Ectopic pole cells are not produced at the anterior of embryos derived from aub^N11/aub^HN ; osk^short.bcd females.

(Harris and Macdonald, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

aub^HN2/aub^QC42 is partially rescued by Scer\GAL4^{VP16.nanos.UTR}/aub^UASp.mGFP6

(Barckmann et al., 2015)

Not rescued by

aub^HN2/aub^QC42 is not rescued by Scer\GAL4^{VP16.nanos.UTR}/aub^{AA.UASp.mGFP6}

(Barckmann et al., 2015)

Comments

The maternal effect embryonic lethality and dorsal appendages fusion phenotypes characteristic for embryos from aub^HN2/aub^QC42 mothers are rescued to a varying extent by expression of either aub^{Scer\UAS.P\T.T:Avic\GFP-m6} or aub^{AA.Scer\UAS.P\T.T:Avic\GFP-m6} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the embryos.

(Barckmann et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8517

aub^HN2 cn¹ bw¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 2 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AF334408

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

AAK43547

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Aub^HN2

(Wakisaka et al., 2019, Yang et al., 2015)

Aub^HN

(Huang et al., 2021)

aub^HN2

(Chavan et al., 2025, Adashev et al., 2024, Ramat et al., 2024, Rojas-Ríos et al., 2024, Curnutte et al., 2023, Iki et al., 2023, Li et al., 2023, Lin et al., 2023, Venkei et al., 2023, Lim et al., 2022, Chen et al., 2021, Cui et al., 2021, Gebert et al., 2021, Huang et al., 2021, Vrettos et al., 2021, Ramat et al., 2020, Stolyarenko, 2020, Kotov et al., 2019, Coux et al., 2018, Ma et al., 2017, Teixeira et al., 2017, Andress et al., 2016, Guida et al., 2016, Lo et al., 2016, Sahin et al., 2016, Sarov et al., 2016, Barckmann et al., 2015, Wang et al., 2015, Ma et al., 2014, Patil et al., 2014, Perrat et al., 2013, Anand and Kai, 2012, Pöyhönen et al., 2012, Becalska et al., 2011, Pek and Kai, 2011, Pek and Kai, 2011, Zamparini et al., 2011, Zhang et al., 2011, Creed et al., 2010, Nagao et al., 2010, Patil and Kai, 2010, Rouget et al., 2010, Li et al., 2009, Lim et al., 2009, Pek et al., 2009, Anne et al., 2007, Josse et al., 2007, Klattenhoff et al., 2007, Lim and Kai, 2007, Lim and Kai, 2007, Pelisson et al., 2007, Grimaud et al., 2006, Vagin et al., 2006, Anne and Mechler, 2005, Cook et al., 2004, Snee and Macdonald, 2004, Tomari et al., 2004, Findley et al., 2003, Kennerdell et al., 2002, Harris and Macdonald, 2001, Wilson et al., 1996, Wilson et al., 1996, Schupbach and Wieschaus, 1991)

aub^HN

(Kina et al., 2025, Eichler et al., 2020, Wang et al., 2020, Ma et al., 2017, Bozzetti et al., 2015, Atikukke et al., 2014, Wylie et al., 2014, Gell and Reenan, 2013, Kelleher et al., 2012, Menon and Meller, 2012, Kibanov et al., 2011, Orsi et al., 2010, Shpiz et al., 2009, Chen et al., 2007, Chen et al., 2007, Ashraf et al., 2006)

aubHN2

(Brennecke et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (84)

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