FlyBase Allele Report: Dmel\shg[UAS.sgGFP]

General Information

Symbol

Dmel\shg^UAS.sgGFP

Species

D. melanogaster

Name

FlyBase ID

FBal0103570

Feature type

allele

Associated gene

Dmel\shg

Associated Insertion(s)

Carried in Construct

P{UAS-shg.DEFL}

Also Known As

UAS-DEFL, UAS-DE-cad-GFP, UAS-E-Cadherin-GFP, UAS-E-Cad-GFP, UAS-DE-Cadherin:GFP, UAS-de-cadherin-GFP, DE-Cadherin-GFP, e-cad-GFP

Key Links

Transgenic product class

cDNA

(Oda and Tsukita, 1999)

wild_type

(Oda and Tsukita, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Oda and Tsukita, 1999)

wild_type

(Oda and Tsukita, 1999)

Mutagen

in vitro construct

(Oda and Tsukita, 1999)

Progenitor genotype

Carried in construct

P{UAS-shg.DEFL}

Cytology

Description

UASt regulatory sequences drive expression of a full-length shg cDNA. The open reading frame is tagged with sgGFP fluorescent protein.

(Oda and Tsukita, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

shg

Tagged with

sgGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Src64B^UAS.cCa

(Enomoto et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal wound healing | embryonic stage, with Scer\GAL4^{VP16.mat.αTub67C}

(Hunter et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of shg^UAS.sgGFP under the control of Scer\GAL4^Abd-B-LDN leads to male pupal A8a epithelia cells showing reduced junction growth rate, as compared to controls.

(Uechi and Kuranaga, 2019)

Overexpression of shg^{Scer\UAS.T:Avic\GFP-rs} in the embryonic epidermis under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in delayed wound closure compared with wild-type.

(Hunter et al., 2015)

The expression of shg^{Scer\UAS.T:Avic\GFP-rs} alone under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} has no obvious effect on the germline stem cell population in adult testes, as compared to controls.

(Liu et al., 2015)

shg^{Scer\UAS.T:Avic\GFP-rs} driven by Scer\GAL4^nos.PU does not affect average germline stem cell number in third instar larval testes.

(Tarayrah et al., 2015)

Male germline stem cell clones expressing shg^{Scer\UAS.T:Avic\GFP-rs} under the control of Scer\GAL4^nos.PS show a considerably higher frequency of symmetric self renewal compared to controls.

(Salzmann et al., 2013)

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} under the control of Scer\GAL4^AB1 causes a bulging phenotype on each cell of the larval salivary gland, but with a normal size of the lumen.

(Lee and Thomas, 2011)

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} under the control of Scer\GAL4^GMR.PF results in low level failure to switch cone-cell contacts.

(Seppa et al., 2008)

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} under the control of Scer\GAL4^btl.PS delays the elongation of the dorsal branches. The tube remains multicellular in the late stage of elongation rather than becoming unicellular, but tracheal development is eventually completed.

(Shindo et al., 2008)

Embryos expressing shg^{Scer\UAS.T:Avic\GFP-rs} under the control of Scer\GAL4^fkh.PH form normal salivary glands.

(Pirraglia et al., 2006)

Clones of shg^{Scer\UAS.T:Avic\GFP-rs}; Scer\GAL4^Act5C.PI cells in the pupal retina (at 40 hours after puparium formation) have little effect on the morphology of cone cells or primary pigment cells. However, when anterior and posterior cone cells are shg^{Scer\UAS.T:Avic\GFP-rs}; Scer\GAL4^Act5C.PI, an interface sometimes forms between these cone cells instead of between dorsal and ventral cones cells as in wild-type.

(Hayashi and Carthew, 2004)

When transiently transformed with Scer\GAL4^Act5C.PHb into S2 cells, promoted a strong cell aggregation phenotype.

(Oda and Tsukita, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

shg^UAS.sgGFP/Scer\GAL4^nanos.PU is a suppressor of decreased cell number | third instar larval stage phenotype of Df(2L)BSC184/Kdm5¹⁰⁴²⁴

(Tarayrah et al., 2015)

shg^UAS.sgGFP, Scer\GAL4^GMR.PF is a suppressor of abnormal planar polarity phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

Scer\GAL4^da.PU/shg^UAS.sgGFP is a suppressor of visible phenotype of PDZ-GEF³/PDZ-GEF⁴

(Singh et al., 2006)

NOT Suppressor of

Statement

Reference

shg^UAS.sgGFP, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of decreased cell number | adult stage phenotype of Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

shg^UAS.sgGFP/Scer\GAL4^nanos.PU is a non-suppressor of decreased occurrence of cell division | third instar larval stage phenotype of Df(2L)BSC184/Kdm5¹⁰⁴²⁴

(Tarayrah et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^AB1, shg^UAS.sgGFP has embryonic/larval salivary gland phenotype, suppressible by Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^AB1

(Lee and Thomas, 2011)

Enhancer of

Statement

Reference

shg^UAS.sgGFP/Scer\GAL4^GMR.PF is an enhancer of cone cell | pupal stage phenotype of Scer\GAL4^GMR.PF, pyd^RNAi.3.UAS

(Seppa et al., 2008)

Suppressor of

Statement

Reference

shg^UAS.sgGFP/Scer\GAL4^nanos.PU is a suppressor of male germline stem cell | third instar larval stage phenotype of Df(2L)BSC184/Kdm5¹⁰⁴²⁴

(Tarayrah et al., 2015)

shg^UAS.sgGFP, Scer\GAL4^AB1 is a suppressor | partially of embryonic/larval salivary gland phenotype of Pak^{UAS.Tag:Myr(Src64B)}, Scer\GAL4^AB1

(Lee and Thomas, 2011)

shg^UAS.sgGFP/Scer\GAL4^hs.PU is a suppressor of egg chamber phenotype of Inx2^FA42

(Mukai et al., 2011)

shg^UAS.sgGFP, Scer\GAL4^GMR.PF is a suppressor of primary pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

shg^UAS.sgGFP, Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

shg^UAS.sgGFP, Scer\GAL4^GMR.PF is a suppressor of pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

shg^UAS.sgGFP, Scer\GAL4^GMR.PF is a suppressor of secondary pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

shg^UAS.sgGFP, Scer\GAL4^GMR.PF is a suppressor of tertiary pigment cell phenotype of Scer\GAL4^GMR.PF, cindr^{RNAi.PC.PD.UAS}

(Johnson et al., 2008)

shg^UAS.sgGFP/Scer\GAL4^btl.PS is a suppressor of embryonic/larval tracheal section phenotype of Scer\GAL4^btl.PS, Src42A^CA.UAS

(Shindo et al., 2008)

shg^UAS.sgGFP, Scer\GAL4^fkh.PH is a suppressor | partially of presumptive embryonic salivary gland phenotype of Rac1^V12.UAS, Scer\GAL4^fkh.PH

(Pirraglia et al., 2006)

Scer\GAL4^da.PU/shg^UAS.sgGFP is a suppressor of spermatheca | increased number phenotype of PDZ-GEF³/PDZ-GEF⁴

(Singh et al., 2006)

Scer\GAL4^da.PU/shg^UAS.sgGFP is a suppressor of eye phenotype of PDZ-GEF³/PDZ-GEF⁴

(Singh et al., 2006)

Scer\GAL4^da.PU/shg^UAS.sgGFP is a suppressor of wing phenotype of PDZ-GEF³/PDZ-GEF⁴

(Singh et al., 2006)

shg^UAS.sgGFP/Scer\GAL4^upd1.PU is a suppressor of cell-cell adherens junction & hub cell phenotype of PDZ-GEF³/PDZ-GEF⁴

(Wang et al., 2006)

NOT Suppressor of

Statement

Reference

shg^UAS.sgGFP, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of testis | adult stage phenotype of Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

shg^UAS.sgGFP, Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of male germline stem cell | adult stage phenotype of Mgtor^GD13871, Scer\GAL4^{VP16.nanos.UTR}

(Liu et al., 2015)

Scer\GAL4^{VP16.nanos.UTR}/shg^UAS.sgGFP is a non-suppressor of male germline stem cell phenotype of chic⁰¹³²⁰/chic¹¹

(Shields et al., 2014)

Scer\GAL4^{VP16.nanos.UTR}/shg^UAS.sgGFP is a non-suppressor of male germline stem cell phenotype of chic⁰¹³²⁰/chic²²¹

(Shields et al., 2014)

Scer\GAL4^{VP16.nanos.UTR}/shg^UAS.sgGFP is a non-suppressor of testis | somatic clone phenotype of His2Av⁸¹⁰

(Morillo Prado et al., 2013)

Scer\GAL4^{VP16.nanos.UTR}/shg^UAS.sgGFP is a non-suppressor of male germline stem cell | somatic clone phenotype of His2Av⁸¹⁰

(Morillo Prado et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of shg^{Scer\UAS.T:Avic\GFP-rs} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the decreased germline stem cell numbers present in the testis of adults expressing either Mtor^GD13871 or Mtor^HMS00735 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Liu et al., 2015)

shg^{Scer\UAS.T:Avic\GFP-rs} driven by Scer\GAL4^nos.PU suppresses the reduced number of germline stem cells seen in lid¹⁰⁴²⁴/Df(2L)BSC184 third instar larval testes.

(Tarayrah et al., 2015)

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} in germ cells under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the germline stem cell (GSC) loss seen in chic¹¹/chic⁰¹³²⁰ mutant testes.

(Shields et al., 2014)

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} in germline stem cells (GSCs) under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} does not suppress the GSC loss seen in His2Av⁸¹⁰ mutant testes.

(Morillo Prado et al., 2013)

Co-expression of shg^{Scer\UAS.T:Avic\GFP-rs} with Pak^{Scer\UAS.T:Myr1} under the control of Scer\GAL4^AB1 completely suppresses the bulging phenotype, and partially suppresses the Pak^{Scer\UAS.T:Myr1} salivary gland lumen expansion phenotype.

(Lee and Thomas, 2011)

Co-expression of shg^{Scer\UAS.T:Avic\GFP-rs} does not significantly change the impaired epidermal cell elongation phenotype seen during dorsal closure stages in embryos expressing Rab11^{S25N.Scer\UAS} under the control of Scer\GAL4^en-e16E.

(Mateus et al., 2011)

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} under the control of Scer\GAL4^hs.PU partially rescues the formation of egg chambers in Inx2^FA42 females.

(Mukai et al., 2011)

Ectopic expression of shg^{Scer\UAS.T:Avic\GFP-rs} strongly suppresses the Scer\GAL4^GMR.PF>cindr^{dsRNA.PC.PD.Scer\UAS} mispatterning phenotype and reduces the distance between interommatidial precursor cells.

(Johnson et al., 2008)

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} strongly enhances the cone cell switching phenotype seen 41-42 hours after pupal formation when pyd^{3.dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PF. A greater proportion of ommatidia maintain the immature A/P configuration rather than switching to the E/P configuration as is seen in controls.

(Seppa et al., 2008)

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} partially suppresses the tracheal defects seen when Src42A^CA.Scer\UAS is expressed under the control of Scer\GAL4^btl.PS. Tracheal cells maintain apico-basal polarity for at least two hours longer than when Src42A^CA.Scer\UAS is expressed alone, and dorsal branch extension is also partially restored.

(Shindo et al., 2008)

The salivary gland defects seen in embryos expressing Rac1^V12.Scer\UAS under the control of Scer\GAL4^fkh.PH are partially suppressed by co-expression of shg^{Scer\UAS.T:Avic\GFP-rs}.

(Pirraglia et al., 2006)

Expression of shg^{Scer\UAS.T:Avic\GFP-rs} driven by Scer\GAL4^da.PU almost fully suppresses the supernumerary spermathecae phenotype (as well as wing and eye phenotypes) in PDZ-GEF⁴/PDZ-GEF³ flies.

(Singh et al., 2006)

shg^{Scer\UAS.T:Avic\GFP-rs}; Scer\GAL4^upd rescues the formation of adherens junctions between male germline stem cells and hub cells in the germinal proliferation center of Gef26⁴/Gef26³.

(Wang et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

shg^UAS.sgGFP/Scer\GAL4^{VP16.mat.αTub67C} rescues shg^R758

(Taniguchi et al., 2011)

shg^UAS.sgGFP/Scer\GAL4^455.2 rescues shg^R758

(Taniguchi et al., 2011)

shg^UAS.sgGFP/Scer\GAL4^btl.PS rescues Df(2R)E2/shg²

(Oda and Tsukita, 1999)

Fails to rescue

shg^UAS.sgGFP/Scer\GAL4^48Y fails to rescue shg^R758

(Taniguchi et al., 2011)

Scer\GAL4^how-24B/shg^UAS.sgGFP fails to rescue shg^R758

(Taniguchi et al., 2011)

Comments

The defects in the left-right asymmetry of the hindgut which are seen in homozygous shg^R758 embryos are rescued by expression of shg^{Scer\UAS.T:Avic\GFP-rs} under the control of either Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} or Scer\GAL4^455.2, but are not rescued by expression of shg^{Scer\UAS.T:Avic\GFP-rs} under the control of either Scer\GAL4^48Y or Scer\GAL4^how-24B.

(Taniguchi et al., 2011)

The addition of shg^{Scer\UAS.T:Avic\GFP-rs}, driven by Scer\GAL4^btl.PS, into shg²/Df(2R)E2 flies rescues their tracheal fusion phenotype.

(Oda and Tsukita, 1999)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

109004

w^*; P{UAS-shg.DEFL}6-3

Notes on Origin

Discoverer

Comments

Carried in a plasmid and co transfected with Scer\GAL4^Act5C.PHb into S2 cells.

(Oda and Tsukita, 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

shg^{Scer\UAS.T:Avic\GFP-rs}

shg^UAS.rsGFP

shg^UAS.sgGFP

Name Synonyms

Secondary FlyBase IDs

References (46)

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