FlyBase Allele Report: Dmel\dachs[GC13]

General Information

Symbol

Dmel\dachs^GC13

Species

D. melanogaster

Name

FlyBase ID

FBal0128007

Feature type

allele

Associated gene

Dmel\dachs

Associated Insertion(s)

Carried in Construct

Also Known As

d^GC13

Key Links

Allele class

loss of function allele

Mutagen

gamma ray

Nature of the Allele

Allele class

loss of function allele

(Mao et al., 2006, Katz, 2001.12.11)

Mutagen

gamma ray

(Katz, 2001.12.11)

Progenitor genotype

Cytology

Description

Contains an 11bp deletion, predicted to cause a truncation of the expressed product at R83 near the N terminus of the head domain, causing a frameshift closely followed by a stop codon.

(Mao et al., 2006)

11bp deletion in the myosin head domain, resulting in a frameshift and stop codon.

(Katz, 2001.12.11)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates dentatorubral-pallidoluysian atrophy

modeled by Gug^75QN.UAS

(Napoletano et al., 2011)

ameliorates neurodegenerative disease

modeled by ft⁸

(Napoletano et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity

(Mao et al., 2006)

abnormal planar polarity (with Df(2L)ED623)

(Matakatsu and Blair, 2008)

abnormal planar polarity | adult stage (with dachs¹)

(Brittle et al., 2012)

abnormal size

(Rauskolb et al., 2011)

decreased size | larval stage

(Matakatsu and Fehon, 2024)

lethal

(Zhang et al., 2016)

lethal - all die before end of P-stage

(Mao et al., 2006)

some die during pharate adult stage

(Mao et al., 2006)

visible

(Mao et al., 2006)

visible (with dachs²¹⁰)

(Mao et al., 2006)

visible (with Df(2L)ED623)

(Mao et al., 2006)

visible (with Df(2L)N22-5)

(Mao et al., 2006)

visible | adult stage | somatic clone

(Gaspar et al., 2015)

Phenotype Manifest In

abdominal tergite marginal bristle (with dachs²¹⁰)

(Misra and Irvine, 2016)

adult abdomen (with Df(2L)ED623)

(Matakatsu and Blair, 2008)

crossvein

(Mao et al., 2006)

crossvein (with dachs¹)

(Mao et al., 2006)

crossvein | somatic clone

(Mao et al., 2006)

eye | adult stage | somatic clone

(Gaspar et al., 2015)

eye disc | third instar larval stage (with dachs¹)

(Gaspar et al., 2015)

leg

(Mao et al., 2006)

leg (with dachs²¹⁰)

(Mao et al., 2006)

leg (with Df(2L)ED623)

(Mao et al., 2006)

(Mao et al., 2006)

(Mao et al., 2006)

(Mao et al., 2006)

(Mao et al., 2006)

ommatidium | somatic clone

(Mao et al., 2006)

tarsal segment

(Mao et al., 2006)

tarsal segment | somatic clone

(Mao et al., 2006)

wing

(Rauskolb et al., 2011, Mao et al., 2006)

wing (with Df(2L)N22-5)

(Mao et al., 2006)

wing | proximal (with dachs¹)

(Brittle et al., 2012)

wing disc

(Matakatsu and Fehon, 2024)

wing disc | conditional

(Grusche et al., 2011)

wing disc | third instar larval stage (with dachs¹)

(Gaspar et al., 2015)

wing hair

(Mao et al., 2006)

wing hair (with dachs¹)

(Brittle et al., 2012)

wing hair (with Df(2L)ED623)

(Matakatsu and Blair, 2008)

Detailed Description

Statement

Reference

d^GC13/d²¹⁰ transheterozygotes display some hair orientation defects in the posterior dorsal abdomen, as compared to controls.

(Misra and Irvine, 2016)

d^GC13/d^GC13 adult eyes (created by the EGUF method in otherwise heterozygous animals) are significantly smaller compared to wild-type.

d¹/d^GC13 transheterozygote third instar larvae have smaller eye and wing discs compared to wild-type.

(Gaspar et al., 2015)

d^GC13/d¹ wing discs irradiated with 40Gy gamma-irradiation display a reduced regenerative capacity compared to un-irradiated controls.

(Grusche et al., 2011)

Mutants have small wings.

(Rauskolb et al., 2011)

d^GC13/Df(2L)ED623 flies show planar cell polarity (PCP) defects in the wing. The animals also have abdominal PCP defects; polarity is almost normal in the anterior compartment, abnormal near the anterior/posterior compartment boundary and reversed in the posterior compartment.

(Matakatsu and Blair, 2008)

The legs of d^GC13 pharate adults are shorter than wild-type legs; the intermediate and distal segments of the leg (femur through tarsus) are noticeably shortened, and some tarsal segments are fused, typically resulting in the formation of only three tarsal segments instead of the normal five. In some cases no external leg tissue is evident, or the leg appears to form a single mass of tissue. Pupal legs, however, are consistently shortened but never absent or severely deformed. Examination of pupae shows that leg absence results from a failure of disc eversion.

The legs of d^GC13/Df(2L)ED623 and d^GC13/d²¹⁰ flies are smaller than wild-type legs.

d^GC13 mutant clones in the leg cause reduced growth and fusion of tarsal segments, but do not cause disc eversion failure.

The wings of d^GC13 adults are small compared to wild type and exhibit abnormal wing patterning, as evidenced by a variable phenotype that includes extra, missing and mis-positioned crossveins. Extra or missing crossveins are often observed in adult flies with d^GC13 clones in the wing, and with d¹/d^GC13 animals.

The wings of d^GC13/Df(2L)N22-5 are smaller than wild type while the wings of d^GC13/Df(2L)ED623 are relatively normal.

d^GC13 flies show a mild polarity phenotype. As in wild-type animals, hairs in the abdomen point posteriorly, most leg bristles and wing hairs point distally and most ommatidia are correctly oriented.

In d^GC13 mutant clones in the abdomen, hair polarity appears normal. d^GC13 clones in the eye show some disorganization of ommatidial orientation.

(Mao et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

dachs^GC13 has visible | adult stage phenotype, enhanceable by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

Scer\GAL4^ey.PH, dachs^GC13, ft^G-rv has abnormal planar polarity | adult stage | somatic clone phenotype, enhanceable by yki^UAS.cHa/Scer\GAL4^ey.PH

(Brittle et al., 2012)

dachs^GC13 has abnormal size phenotype, enhanceable by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

NOT Enhanced by

Statement

Reference

dachs¹/dachs^GC13 has abnormal planar polarity | adult stage phenotype, non-enhanceable by ds^38k/ds^UAO71

(Brittle et al., 2012)

NOT suppressed by

Statement

Reference

dachs¹/dachs^GC13 has abnormal planar polarity | adult stage phenotype, non-suppressible by ds^38k/ds^UAO71

(Brittle et al., 2012)

NOT Enhancer of

Statement

Reference

dachs^GC13/dachs^GC13 is a non-enhancer of abnormal size | late third instar larval stage phenotype of Dlish^UAS.Tag:FLAG, Scer\GAL4^hh-Gal4

(Wang et al., 2019)

Suppressor of

Statement

Reference

dachs^GC13 is a suppressor of increased size | larval stage phenotype of ds^UAO71, ft⁸/ft^G-rv

(Matakatsu and Fehon, 2024)

dachs¹/dachs^GC13 is a suppressor | partially of abnormal planar polarity | adult stage phenotype of ds^38k/ds^UAO71

(Brittle et al., 2012)

dachs¹/dachs^GC13 is a suppressor of increased size | adult stage phenotype of ds^38k/ds^UAO71

(Brittle et al., 2012)

dachs¹/dachs^GC13 is a suppressor of abnormal planar polarity | somatic clone | adult stage phenotype of Scer\GAL4^Act5C.PI, ds^UAS.cTa

(Brittle et al., 2012)

dachs^GC13/dachs^GC13 is a suppressor | partially of abnormal planar polarity | somatic clone | adult stage phenotype of Scer\GAL4^ey.PH, ft^G-rv

(Brittle et al., 2012)

dachs^GC13 is a suppressor of abnormal neuroanatomy | somatic clone | adult stage | progressive phenotype of ft⁸

(Napoletano et al., 2011)

dachs^GC13 is a suppressor of abnormal cell polarity | somatic clone phenotype of ft⁸

(Mao et al., 2006)

NOT Suppressor of

Statement

Reference

dachs^GC13/dachs^GC13 is a non-suppressor of abnormal size | late third instar larval stage phenotype of Dlish^UAS.Tag:FLAG, Scer\GAL4^hh-Gal4

(Wang et al., 2019)

dachs^GC13 is a non-suppressor of increased cell number | larval stage phenotype of ex^e1

(Cho et al., 2006)

Other

Statement

Reference

Dlish^UAS.Tag:FLAG, Scer\GAL4^hh-Gal4, dachs¹/dachs^GC13 has visible | adult stage phenotype

(Wang et al., 2019)

Dlish^UAS.Tag:FLAG, Scer\GAL4^hh-Gal4, dachs¹/dachs^GC13 has abnormal size | adult stage phenotype

(Wang et al., 2019)

Scer\GAL4^Tub.PU, dachs¹/dachs^GC13, ft⁸/ft^G-rv, wts^UAS.cUa has abnormal planar polarity | adult stage phenotype

(Brittle et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

dachs¹/dachs^GC13 has eye disc | third instar larval stage phenotype, enhanceable by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

dachs¹/dachs^GC13 has wing disc | third instar larval stage phenotype, enhanceable by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

dachs^GC13 has eye | adult stage | somatic clone phenotype, enhanceable by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

Scer\GAL4^ey.PH, dachs^GC13, ft^G-rv has ommatidium | adult stage | somatic clone phenotype, enhanceable by yki^UAS.cHa/Scer\GAL4^ey.PH

(Brittle et al., 2012)

dachs^GC13 has wing phenotype, enhanceable by Zyx^NIG.32018R/Scer\GAL4^nub-AC-62

(Rauskolb et al., 2011)

NOT Enhanced by

Statement

Reference

dachs¹/dachs^GC13 has wing hair phenotype, non-enhanceable by ds^38k/ds^UAO71

(Brittle et al., 2012)

dachs¹/dachs^GC13 has wing | proximal phenotype, non-enhanceable by ds^38k/ds^UAO71

(Brittle et al., 2012)

NOT suppressed by

Statement

Reference

dachs¹/dachs^GC13 has wing hair phenotype, non-suppressible by ds^38k/ds^UAO71

(Brittle et al., 2012)

dachs¹/dachs^GC13 has wing | proximal phenotype, non-suppressible by ds^38k/ds^UAO71

(Brittle et al., 2012)

NOT Enhancer of

Statement

Reference

dachs^GC13/dachs^GC13 is a non-enhancer of wing disc posterior compartment | late third instar larval stage phenotype of Dlish^UAS.Tag:FLAG, Scer\GAL4^hh-Gal4

(Wang et al., 2019)

Suppressor of

Statement

Reference

dachs^GC13 is a suppressor of wing disc phenotype of ds^UAO71, ft⁸/ft^G-rv

(Matakatsu and Fehon, 2024)

dachs¹/dachs^GC13 is a suppressor | partially of wing hair phenotype of ds^38k/ds^UAO71

(Brittle et al., 2012)

dachs¹/dachs^GC13 is a suppressor | partially of wing phenotype of ds^38k/ds^UAO71

(Brittle et al., 2012)

dachs¹/dachs^GC13 is a suppressor | partially of ommatidium | adult stage phenotype of ds^38k/ds^UAO71

(Brittle et al., 2012)

dachs¹/dachs^GC13 is a suppressor of ommatidium | somatic clone | adult stage phenotype of Scer\GAL4^Act5C.PI, ds^UAS.cTa

(Brittle et al., 2012)

dachs^GC13/dachs^GC13 is a suppressor | partially of ommatidium | somatic clone | adult stage phenotype of Scer\GAL4^ey.PH, ft^G-rv

(Brittle et al., 2012)

dachs^GC13 is a suppressor of eye photoreceptor cell | somatic clone | progressive phenotype of ft⁸

(Napoletano et al., 2011)

dachs^GC13 is a suppressor of eye photoreceptor cell phenotype of Gug^75QN.UAS, Scer\GAL4^ninaE.PT

(Napoletano et al., 2011)

dachs^GC13 is a suppressor of leg | somatic clone phenotype of ft⁸

(Mao et al., 2006)

dachs^GC13 is a suppressor of microchaeta | somatic clone phenotype of ft⁸

(Mao et al., 2006)

NOT Suppressor of

Statement

Reference

dachs^GC13/dachs^GC13 is a non-suppressor of wing disc posterior compartment | late third instar larval stage phenotype of Dlish^UAS.Tag:FLAG, Scer\GAL4^hh-Gal4

(Wang et al., 2019)

dachs^GC13 is a non-suppressor of wing disc | larval stage phenotype of ex^e1

(Cho et al., 2006)

dachs^GC13 is a non-suppressor of ommatidium | somatic clone phenotype of ft⁸

(Mao et al., 2006)

Other

Statement

Reference

Dlish^UAS.Tag:FLAG, Scer\GAL4^hh-Gal4, dachs¹/dachs^GC13 has wing phenotype

(Wang et al., 2019)

Scer\GAL4^Tub.PU, dachs¹/dachs^GC13, ft⁸/ft^G-rv, wts^UAS.cUa has ommatidium | adult stage phenotype

(Brittle et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The small eye phenotype characteristic for d^GC13/d^GC13 adult eyes (created by the EGUF method in otherwise heterozygous animals) can be enhanced by combination with Zyx^Δ41/Zyx^Δ41.

The reduced size of both eye and wing discs characteristic for d¹/d^GC13 transheterozygote third instar larvae is further enhanced by combination with Zyx^Δ41/Zyx^Δ41.

(Gaspar et al., 2015)

d^GC13 suppresses the retinal neurodegeneration seen in ft⁸ clones.

The photoreceptor degeneration caused by Gug^{75QN.Scer\UAS} under the control of Scer\GAL4^ninaE.PT is significantly suppressed inside d^GC13 clones.

(Napoletano et al., 2011)

Scer\GAL4^nub-AC-62-mediated expression of Zyx102EF^NIG.32018R (together with Dcr-2^Scer\UAS.cDa) enhances the small wing phenotype of d^GC13 flies.

(Rauskolb et al., 2011)

d^GC13 does not suppress the wing disc overgrowth seen in wts^P1 mutant larvae.

d^GC13 does not suppress the wing disc overgrowth seen in ex^e1 mutant larvae.

(Cho et al., 2006)

ft⁸, d^GC13 double mutant clones in the leg do not induce outgrowths or the appearance of vesicles in the cuticular tissue, a phenotype seen when ft⁸ single mutant clones are generated in the leg.

d^GC13 partially suppresses the polarity phenotype of ft⁸. This is evidenced by comparison of ft⁸ and d^GC13 single mutant clones with ft⁸, d^GC13 double mutant clones in the abdomen. While all (39/39) ft⁸ clones have a polarity phenotype, only 54% of ft⁸, d^GC13 clones show this phenotype (vs 3% of d^GC13 clones). The ft⁸ single mutant clones exhibit some hairs at an angle greater than 90^o relative to the normal orientation, while only 26% of ft⁸, d^GC13 clones had hairs of this angle.

The polarity phenotype of ft⁸, d^GC13 double mutant clones in the eye is similar to that of ft⁸ single mutant clones (28 vs 32 out of 33 clones including ommatidia rotated more than 90^o away from the normal position, respectively.

The polarity of hairs surrounding ck¹³, d^GC13 clones in the abdomen appear normal.

(Mao et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

dachs^GC13 is rescued by dachs^{ΔN.UAS.Tag:V5}/Scer\GAL4^Tub.PU

(Zhang et al., 2016)

dachs^GC13 is rescued by dachs^{UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^Tub.PU

(Zhang et al., 2016)

dachs²¹⁰/dachs^GC13 is rescued by dachs^GFP

(Bosveld et al., 2012)

dachs²¹⁰/dachs^GC13 is rescued by dachs^+tcBa

(Bosveld et al., 2012)

Partially rescued by

dachs^GC13 is partially rescued by dachs^{UAS.Tag:V5,Tag:polyHis}/Scer\GAL4^Tub.PU

(Mao et al., 2006)

Not rescued by

dachs^GC13 is not rescued by dachs^{ΔMH.UAS.Tag:V5}/Scer\GAL4^Tub.PU

(Zhang et al., 2016)

dachs^GC13 is not rescued by Scer\GAL4^Tub.PU/dachs^{Δ1C.UAS.Tag:V5}

(Zhang et al., 2016)

dachs^GC13 is not rescued by Scer\GAL4^Tub.PU/dachs^{Δ2C.UAS.Tag:V5}

(Zhang et al., 2016)

dachs^GC13 is not rescued by dachs^{Δ3C.UAS.Tag:V5}/Scer\GAL4^Tub.PU

(Zhang et al., 2016)

Comments

Expression of either d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} or d^{ΔN.Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^tub.PU restores the viability of d^GC13 mutant flies, while expresion of any of the following: d^{ΔMH.Scer\UAS.T:SV5\V5}, d^{Δ1C.Scer\UAS.T:SV5\V5}, d^{Δ2C.Scer\UAS.T:SV5\V5}, or d^{Δ3C.Scer\UAS.T:SV5\V5} does not rescue d^GC13 viability.

(Zhang et al., 2016)

d^T:Avic\GFP rescues the morphological defects and viability of d^GC13/d²¹⁰ animals.

d^+tcBa rescues the morphological defects and viability of d^GC13/d²¹⁰ animals.

(Bosveld et al., 2012)

Expression of d^{Scer\UAS.T:SV5\V5,T:Zzzz\His6} under the control of Scer\GAL4^tub provides significant rescue of the wing and leg phenotypes of d^GC13 mutants.

(Mao et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6389

dachs^GC13 pr¹ cn¹/CyO

28289

w^*; dachs^GC13 P{neoFRT}40A/CyO, P{ActGFP}JMR1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

d^G13

(Okumura et al., 2015)

d^GC13

(Zecca and Struhl, 2021, Kumar et al., 2020, Wang et al., 2019, Matakatsu et al., 2017, Bosveld et al., 2016, Misra and Irvine, 2016, Saavedra et al., 2016, Gaspar et al., 2015, Okumura et al., 2015, Vrabioiu and Struhl, 2015, Kawamori et al., 2011, Napoletano et al., 2011, Rauskolb et al., 2011, Matakatsu and Blair, 2008, Christensen and Cook, 2006.8.30, Mao et al., 2006)

dachs^<up>GC13</up>

(Bosveld et al., 2012)

dachs^GC13

(Matakatsu and Fehon, 2024, Nagai et al., 2021, Arata et al., 2017, Zhang et al., 2016, Brittle et al., 2012, Grusche et al., 2011)

Name Synonyms

dachsGC13

(Degoutin et al., 2013, Cho et al., 2006)

Secondary FlyBase IDs

References (26)

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