FlyBase Allele Report: Scer\GAL4[Gr33a-GAL4]

General Information

Symbol

Scer\GAL4^Gr33a-GAL4

Species

S. cerevisiae

Name

FlyBase ID

FBal0243785

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

TI{GAL4}Gr33a^GAL4

Carried in Construct

Also Known As

Gr33a-Gal4, Gr33a^GAL4

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

recombination

(Moon et al., 2009)

I-SceI

(Moon et al., 2009)

FLPase

(Moon et al., 2009)

ends-out gene targeting

(Moon et al., 2009, Moon et al., 2009)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}Gr33a^GAL4

Cytology

Description

Sequence encoding a GAL4 driver has been inserted at the site of the normal translation initiation codon of the Gr33a locus (a mini-w marker has also been inserted in addition to the GAL4 coding region and a portion of the endogenous Gr33a coding region has been replaced by the inserted sequences).

(Moon et al., 2009)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

gustatory receptor neuron B2 of the terminal organ dorsolateral group

(Mast et al., 2014)

third instar larval stage

abdominal 4 ventral multidendritic neuron v'td2

(Qian et al., 2018)

abdominal 5 ventral multidendritic neuron v'td2

(Qian et al., 2018)

abdominal 6 ventral multidendritic neuron v'td2

(Qian et al., 2018)

gustatory receptor neuron B1 of the terminal organ dorsolateral group

(Mast et al., 2014)

larval dorsal pharyngeal gustatory receptor neuron DP2

(Rass et al., 2019)

adult stage

bitter-sensing neuron of the leg

(Koh et al., 2014)

bitter-sensitive labellar taste bristle

(Shim et al., 2015)

intermediate labellar taste bristle

(Poudel et al., 2015, Shim et al., 2015)

short labellar taste bristle

(Poudel et al., 2015)

short labellar taste bristle S-a

(Sung et al., 2017)

short labellar taste bristle S-b

(Sung et al., 2017)

bitter-sensing neuron of labellar taste bristle

(Rass et al., 2019)

bitter-sensing neuron

(Deere et al., 2023, Zhang et al., 2020)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

gustatory receptor neuron of the labellum | subset

(Moon et al., 2009)

prothoracic leg taste bristle chemosensory neuron | subset

(Moon et al., 2009)

metathoracic leg taste bristle chemosensory neuron | subset

(Moon et al., 2009)

mesothoracic leg taste bristle chemosensory neuron | subset

(Moon et al., 2009)

gustatory receptor neuron | subset of tarsal segment

(Moon et al., 2009)

Additional Information

Statement

Reference

ScerGAL4^Gr33a-GAL4 drives expression in bitter-sensing neurons in the larval dorsal pharyngeal organ.

(Rass et al., 2019)

Drives expression in bitter-activated gustatory receptor neurons in the adult labellum and in the tarsal segments of legs.

(FlyBase Curators, 2017)

ScerGAL4^Gr33a-GAL4 drives expression in S-a and S-b bitter-activated gustatory receptor neurons in the adult labellum.

(Sung et al., 2017)

ScerGAL4^Gr33a-GAL4 drives expression in L-canavanine-insensitive S2- and I-type sensilla.

(Shim et al., 2015)

ScerGAL4^Gr33a-GAL4 drives expression in all I-type and S-type sensilla, which represent a total of 22 gustatory receptor neurons in each labellum.

(Poudel et al., 2015)

ScerGAL4^Gr33a-GAL4 drives expression in two of the three gustatory receptor neurons innervating the dorsolateral terminal organ. These neurons coexpress ppk23, but not ScerGAL4^GMR58F10

(Mast et al., 2014)

ScerGAL4^Gr33a-GAL4 drives expression in about 20 gustatory receptor neurons (GRNs) in the adult labellum; these GRNs coexpress Gr66a. ScerGAL4^Gr33a-GAL4 also drives expression in multiple gustory receptor neurons in the tarsal segments of the legs; these include 7 in each prothoracic leg, 4 in each mesothoracic leg, and 4 in each methoracic leg. Several of the prothoracic GRNs coexpress ScerGAL4^Gr32a.3.776.

(Moon et al., 2009)

Marker for

bitter-sensing neuron

(Deere et al., 2023, Zhang et al., 2020)

Reflects expression of

Gr33a

(Deere et al., 2023, Zhang et al., 2020, Bloomington Drosophila Stock Center, 2017, Koh et al., 2014, Mast et al., 2014, Moon et al., 2009)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | adult stage, with Ctet\tetX^TNT-LC.UAS

(Ahn et al., 2017)

abnormal chemosensory behavior | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Pradhan et al., 2025, Pradhan et al., 2024, Ahn et al., 2017)

abnormal chemosensory behavior | adult stage, with Ir7g^KK105053

(Pradhan et al., 2025)

abnormal chemosensory behavior | adult stage, with Ir25a^KK106802

(Pradhan et al., 2025)

abnormal chemosensory behavior | adult stage, with Ir51b^GD14815

(Pradhan et al., 2025)

abnormal chemosensory behavior | adult stage, with Ir67a^KK104455

(Pradhan et al., 2025)

abnormal chemosensory behavior | adult stage, with Ir76b^GD2741

(Pradhan et al., 2025)

abnormal courtship behavior, with Hsap\APP^{695.UAS.Tag:MYC}

(Hu et al., 2015)

abnormal neuroanatomy, with Hsap\APP^{695.UAS.Tag:MYC}

(Hu et al., 2015)

abnormal neurophysiology | adult stage, with Gr22e^LL05566, Gr22e^UAS.cSa

(Sung et al., 2017)

abnormal neurophysiology | adult stage, with Gr22e^UAS.cPa

(Aryal and Lee, 2022)

abnormal neurophysiology | adult stage, with Hsap\KCNJ2^UAS.EGFP

(Pradhan et al., 2025, Pradhan et al., 2024)

abnormal neurophysiology | adult stage, with Ir7g^KK105053

(Pradhan et al., 2025)

abnormal neurophysiology | adult stage, with Ir25a^KK106802

(Pradhan et al., 2025)

abnormal neurophysiology | adult stage, with Ir51b^GD14815

(Pradhan et al., 2025)

abnormal neurophysiology | adult stage, with Ir67a^KK104455

(Pradhan et al., 2025)

abnormal neurophysiology | adult stage, with Ir76b^GD2741

(Pradhan et al., 2025)

abnormal taste perception, with Gr8a^GD719

(Shim et al., 2015)

abnormal taste perception, with Gr66a^GD14820

(Shim et al., 2015)

abnormal taste perception | adult stage, with Gr22e^LL05566, Gr22e^UAS.cSa

(Sung et al., 2017)

decreased cell number, with Hsap\APP^{695.UAS.Tag:MYC}

(Hu et al., 2015)

increased mortality | chemical conditional, with Hsap\KCNJ2^UAS.EGFP

(Pradhan et al., 2024)

Phenotype Manifest In

adult gustatory receptor neuron, with Hsap\KCNJ2^UAS.EGFP

(Pradhan et al., 2025)

intermediate labellar taste bristle, with Gr22e^LL05566, Gr22e^UAS.cSa

(Sung et al., 2017)

intermediate labellar taste bristle I-b | adult stage, with Gr22e^UAS.cPa

(Aryal and Lee, 2022)

labellum, with Hsap\APP^{695.UAS.Tag:MYC}

(Hu et al., 2015)

neuron, with Hsap\APP^{695.UAS.Tag:MYC}

(Hu et al., 2015)

prothoracic tarsal segment 4, with Hsap\APP^{695.UAS.Tag:MYC}

(Hu et al., 2015)

prothoracic tarsal segment 5, with Hsap\APP^{695.UAS.Tag:MYC}

(Hu et al., 2015)

short labellar taste bristle, with Gr22e^LL05566, Gr22e^UAS.cSa

(Sung et al., 2017)

short labellar taste bristle S-a, with Hsap\KCNJ2^UAS.EGFP

(Pradhan et al., 2025, Pradhan et al., 2024)

short labellar taste bristle S-a, with Ir7g^KK105053

(Pradhan et al., 2025)

short labellar taste bristle S-a, with Ir25a^KK106802

(Pradhan et al., 2025)

short labellar taste bristle S-a, with Ir51b^GD14815

(Pradhan et al., 2025)

short labellar taste bristle S-a, with Ir67a^KK104455

(Pradhan et al., 2025)

short labellar taste bristle S-a, with Ir76b^GD2741

(Pradhan et al., 2025)

Detailed Description

Statement

Reference

Inhibition (using Ctet\tetX^TNT-LC.UAS or Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^Gr33a-GAL4 cells in adults results in a significant increase in proboscis extensions in response to higher concentrations of hexanoic acid, with no effect on the response to sucrose, compared to controls.

(Ahn et al., 2017)

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of the combined set of Scer\GAL4^Gr33a-GAL4 and Scer\GAL4^Gr64f.9.7 cells in adult females does not affect preference for oviposition on acid-containing medium, compared to controls.

(Chen and Amrein, 2017)

Males in which neurons expressing Gr33a^GAL4 have been silenced show no difference in courtship intensity as compared with wild type or controls, but lose preference for younger females, instead showing no significant preference for either younger or older females.

(Hu et al., 2015)

Inhibition (using Ctet\TeTxLC^TNT.UAS) of Scer\GAL4^Gr33a-GAL4 cells does not affect the preference of larvae for larval-treated substrate, compared to controls.

(Mast et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Gr22e^UAS.cPa, Scer\GAL4^Gr33a-GAL4 has abnormal neurophysiology | adult stage phenotype, non-enhanceable by Gr9a^UAS.cAa/Gr98a^UAS.cAa, Scer\GAL4^Gr33a-GAL4

(Aryal and Lee, 2022)

Suppressed by