FlyBase Gene Report: Dmel\CalpA

General Information

Symbol

Dmel\CalpA

Species

D. melanogaster

Name

Calpain-A

Annotation Symbol

CG7563

Feature Type

protein_coding_gene

FlyBase ID

FBgn0012051

Gene Model Status

Current

Stock Availability

12 publicly available

Enzyme Name (EC)

Gene Summary

Calpain-A (CalpA) encodes a calcium-dependent modulatory protease that cleaves its substrates in a limited fashion. During patterning and division in the blastoderm embryo, CalpA product cleaves the NF-κB inhibitor and the cell cycle regulator encoded by cact and CycB, respectively. CalpA product also regulates dendritic prunning of sensory neurons. [Date last reviewed: 2018-09-13] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

calpain

Key Links

Genomic Location

Cytogenetic map

56D5-56D5

Sequence location

2R:19,425,113..19,430,564 [+]

Recombination map

2-89

RefSeq locus

NT_033778 REGION:19425113..19430564

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables calcium-dependent cysteine-type endopeptidase activity

inferred from direct assay

(Metwally et al., 2021, Fontenele et al., 2013)

inferred from direct assay

(Jekely and Friedrich, 1999)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables calcium ion binding

inferred from electronic annotation with InterPro:IPR002048

(InterPro Project Members, 2004-)

non-traceable author statement

(Jekely and Friedrich, 1999)

enables calcium-dependent cysteine-type endopeptidase activity

inferred from biological aspect of ancestor with PANTHER:PTN000021568

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001300, InterPro:IPR022684

(InterPro Project Members, 2004-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in cuticle development

inferred from direct assay

(Fontenele et al., 2009)

involved_in defense response to fungus

inferred from mutant phenotype

(Fontenele et al., 2013)

involved_in dorsal/ventral pattern formation

inferred from direct assay

(Fontenele et al., 2009)

involved_in larval locomotory behavior

inferred from mutant phenotype

(Buffolo et al., 2015)

involved_in myoblast fusion

inferred from mutant phenotype

(Buffolo et al., 2015)

involved_in neuron remodeling

inferred from mutant phenotype

(Kanamori et al., 2013)

inferred from genetic interaction with FLYBASE:Ca-β; FB:FBgn0287724

(Kanamori et al., 2013)

involved_in protein autoprocessing

inferred from direct assay

(Jekely and Friedrich, 1999)

involved_in proteolysis

inferred from direct assay

(Jekely and Friedrich, 1999)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proteolysis

inferred from biological aspect of ancestor with PANTHER:PTN000021568

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR001300, InterPro:IPR022684

(InterPro Project Members, 2004-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in actin cytoskeleton

inferred from direct assay

(Emori and Saigo, 1994)

is_active_in endoplasmic reticulum

inferred from direct assay

(Metwally et al., 2021)

is_active_in Golgi apparatus

inferred from direct assay

(Metwally et al., 2021)

located_in neuronal cell body

inferred from direct assay

(Theopold et al., 1995)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000021568

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

CALPAINS

Protein Family (UniProt)

Belongs to the peptidase C2 family. (Q11002)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

calcium-dependent cysteine-type endopeptidase activity

Summaries

Gene Snapshot

Gene Group (FlyBase)

CALPAINS -: Calpains are calcium-dependent, cysteine-type endopeptidases that comprise MEROPS peptidase family C2. They play multiple roles in intracellular signal processing by limited proteolysis of target substrate proteins, thereby changing their function. Calpains are involved in numerous different physiological processes such as cell proliferation, migration, invasion, apoptosis and signal transduction. Compared to mammalian Calpains, D. melanogaster CalpA and CalpB are considered 'typical', CalpD (sol) has an atypical structure, while CalpC is a truncated form and is considered inactive because all three active site residues are mutated. (Adapted from FBrf0179911 and PMID:29693408.)

Protein Function (UniProtKB)

Calcium-regulated non-lysosomal thiol-protease. Involved in the organization of the actin-related cytoskeleton during embryogenesis. ACTIVITY REGULATION: Activated by millimolar concentrations of calcium, and by phosphatidylinositol 4,5-diphosphate, phosphatidylinositol 4-monophosphate, phosphatidylinositol and phosphatidic acid.

(UniProt, Q11002)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CalpA for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q11002)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CalpA-RA

FBtr0086530

NM_057700

2301

565

CalpA-RB

FBtr0086529

NM_057699

2797

828

CalpA-RC

FBtr0112869

NM_001103908

2889

828

CalpA-RD

FBtr0340532

NM_001299684

2934

843

Additional Transcript Data and Comments

Reported size (kB)

3.1, 2.4 (northern blot)

(Theopold et al., 1995)

2.7 (longest cDNA)

(Emori and Saigo, 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CalpA-PA

FBpp0085715

64.8

565

4.46

NP_477048

AAF57564

CalpA-PB

FBpp0085714

94.0

828

4.58

FBpp0111782

94.0

828

4.58

FBpp0309435

95.6

843

4.58

A0A0B4LG26

NP_001286613

AHN56408

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

828 aa isoforms: CalpA-PB, CalpA-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

828, 558 (aa); 97 (kD observed); 64 (kD predicted)

(Theopold et al., 1995)

828 (aa); 90 (kD observed)

(Emori and Saigo, 1994)

Comments

External Data

Post Translational Modification

Undergoes calcium-dependent autolytic cleavage between Lys-54 and Asn-55, which is necessary for activation of the protein.

(UniProt, Q11002)

Crossreferences

InterPro - A database of protein families, domains and functional sites

MEROPS - An information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit them.

C02.950

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CalpA using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CalpA_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.81

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal ectoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic foregut

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Theopold et al., 1995)

radioisotope in situ

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval hemocoel

(Theopold et al., 1995)

oogenesis & larval stage

ovary

(Theopold et al., 1995)

adult stage

adult midgut | restricted

(Theopold et al., 1995)

adult proventriculus

(Theopold et al., 1995)

adult central nervous system

(Theopold et al., 1995)

Additional Descriptive Data

CalpA transcripts localize to the central nervous system, cardia, and the epithelial layer of the midgut of adults. In larvae, expression is detected in the hemocoel and in the ovary.

(Theopold et al., 1995)

Both CalpA transcripts are expressed in all developmental stages. They are expressed at approximately equal levels in adults, while the 3.1 kb transcript is four times as abundant as the 2.4 kb transcript in embryos.

(Theopold et al., 1995)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 2

pole bud

(Emori and Saigo, 1994)

organism | anterior

(Emori and Saigo, 1994)

organism | posterior

(Emori and Saigo, 1994)

embryonic cycle 8 -- embryonic cycle 13

• nucleus | surrounding

(Emori and Saigo, 1994)

larval stage

embryonic/larval fat body

(Theopold et al., 1995)

adult stage

adult midgut | restricted

(Theopold et al., 1995)

protocerebrum | restricted

(Theopold et al., 1995)

tritocerebrum | restricted

(Theopold et al., 1995)

adult central nervous system | restricted

(Theopold et al., 1995)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

(Theopold et al., 1995)

Additional Descriptive Data

CalpA protein is expressed in a limited number of cells in the central nervous system, including a cluster of 12-14 large neurons in each hemisphere of the posterior protocerebrum, and a cluster of 10-12 cells in each hemisphere of the anterior tritocerebrum. Expression is also detected in triangular endocrine cells of the open type in the midgut, and some larval fat body cells, possibly hemocytes, which persist into the young adult. In the hemocyte cell line mbn-2, CalpA protein is excluded from the nucleus and vacuoles, and localizes to granular structures that might be secretory granules.

(Theopold et al., 1995)

The 97 kD CalpA protein is detected in Western blots of adult heads and bodies, but the 64 kD protein is rarely detected.

(Theopold et al., 1995)

In the very early embryo, CalpA protein localizes to the anterior and posterior regions of the embryo. The anterior signal disappears during nuclear cleavage, while the posterior signal is visible around the polar buds, and condenses below the pole cells. From cleavage cycles 8 and 9 to cycle 13, CalpA protein localizes between the nuclei at the cell surface. Co-localization with actin reveals that CalpA protein is at the edge of and between actin caps that underlie the plasma membrane immediately above each nucleus.

(Emori and Saigo, 1994)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in actin cytoskeleton

inferred from direct assay

(Emori and Saigo, 1994)

is_active_in endoplasmic reticulum

inferred from direct assay

(Metwally et al., 2021)

is_active_in Golgi apparatus

inferred from direct assay

(Metwally et al., 2021)

located_in neuronal cell body

inferred from direct assay

(Theopold et al., 1995)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CalpA in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CalpA

View exonic expression by tissue for Dmel\CalpA

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0012051

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37232/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0012051

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0012051

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0012051

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7563

Flygut - An atlas of the Drosophila adult midgut

FBgn0012051

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CalpA

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 16 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 12 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CalpA

Transgenic constructs containing regulatory region of CalpA

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)ED3716

(Grabbe et al., 2004)

Gene partially disrupted in

Df(2R)CalpA⁸⁰⁸

(Kókai et al., 2012)

Gene not disrupted in

Df(2R)Fak^K24

(Tsai et al., 2008)

Inferred from location ( 2 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal - majority die, with CalpA^GS9103, Scer\GAL4^Mef2.PR

CalpA^JF03391

partially lethal - majority die, with CalpA^JF03391, Scer\GAL4^Mef2.PR

CalpA^GS9103

partially lethal - majority die, with Scer\GAL4^Mef2.PR

CalpA^GS9103

viable

CalpA⁸⁰⁸

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CalpA^GD12275

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

CalpA^GD12275

viable, with Scer\GAL4^tin.CΔ4

CalpA^GD12275

Other Phenotypes

Allele

abnormal immune response | adult stage, with Scer\GAL4^Cg.PA

CalpA^JF03391

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^C23

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Mef2.PR

CalpA^JF03391

abnormal neuroanatomy | P-stage (with CalpA⁷²)

CalpA⁷⁵

abnormal neuroanatomy | P-stage (with CalpA⁷⁵)

CalpA⁷²

abnormal neuroanatomy | pupal stage, with Scer\GAL4^ppk.PG

decreased size | pupal stage, with Scer\GAL4^ppk.PG

CalpA^UAS.cMa

increased size | pupal stage, with Scer\GAL4^ppk.PG

CalpA^JF03391

long lived, with Scer\GAL4^da.Switch.PT

CalpA^GX16168

majority die during embryonic stage, with Scer\GAL4^Mef2.PR

CalpA^GS9103

Phenotype manifest in

Allele

A1-7 lateral transverse muscle cell, with Scer\GAL4^Mef2.PR

CalpA^JF03391

dendrite | P-stage (with CalpA⁷²)

CalpA⁷⁵

dendrite | P-stage (with CalpA⁷⁵)

CalpA⁷²

dendrite | pupal stage, with Scer\GAL4^ppk.PG

larval multidendritic class IV neuron | P-stage (with CalpA⁷²)

CalpA⁷⁵

larval multidendritic class IV neuron | P-stage (with CalpA⁷⁵)

CalpA⁷²

larval multidendritic class IV neuron | pupal stage, with Scer\GAL4^ppk.PG

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (32)

Hsap\CAPN9

13 of 14

Yes

Hsap\CAPN3

12 of 14

Yes

Hsap\CAPN8

12 of 14

Hsap\CAPN1

11 of 14

Yes

Hsap\CAPN2

11 of 14

Yes

Hsap\CAPN11

11 of 14

Yes

Hsap\CAPN12

6 of 14

Yes

Hsap\CAPN13

6 of 14

Hsap\CAPN14

6 of 14

Yes

Hsap\CAPN5

4 of 14

Yes

Hsap\CAPN6

4 of 14

Yes

Hsap\CAPN10

4 of 14

Yes

Hsap\CAPN15

3 of 14

Hsap\CAPN7

2 of 14

Hsap\ADGB

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (18)

Rnor\Capn9

13 of 14

Yes

Rnor\Capn3

12 of 14

Yes

Rnor\Capn8

12 of 14

Rnor\Capn1

11 of 14

Yes

Rnor\Capn2

10 of 14

Yes

Rnor\Capn11

10 of 14

Yes

Rnor\Capn12

6 of 14

Rnor\Capn13

6 of 14

Rnor\Capn5

4 of 14

Yes

Rnor\Capn6

4 of 14

Yes

Rnor\Capn10

4 of 14

Yes

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (19)

Mmus\Capn9

13 of 14

Yes

Mmus\Capn3

12 of 14

Yes

Mmus\Capn8

12 of 14

Mmus\Capn1

11 of 14

Yes

Mmus\Capn2

11 of 14

Yes

Mmus\Capn11

9 of 14

Yes

Mmus\Capn12

6 of 14

Mmus\Capn13

6 of 14

Mmus\Capn5

5 of 14

Yes

Mmus\Capn6

4 of 14

Yes

Mmus\Capn10

3 of 14

Yes

Mmus\Capn7

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (35)

Xtro\capn3

10 of 13

Yes

Xtro\capn9

8 of 13

Yes

Xtro\capn1

6 of 13

Yes

Xtro\capn2

6 of 13

Yes

Xtro\capn8.1

6 of 13

Yes

Xtro\capn11

6 of 13

Yes

Xtro\capn10l

5 of 13

Yes

Xtro\capn12

5 of 13

Yes

Xtro\capn13

5 of 13

Yes

Xtro\ppp2r5c

5 of 13

Xtro\capn6

4 of 13

Yes

Xtro\capn8.3

4 of 13

Yes

Xtro\capn5

3 of 13

Yes

Xtro\capn14

3 of 13

Yes

Xtro\LOC100495090

3 of 13

Yes

Xtro\LOC100495980

3 of 13

Yes

Xtro\XB5844015

3 of 13

Yes

Xtro\capn8.5

2 of 13

Yes

Xtro\capn10

2 of 13

Yes

Xtro\tnfsf10l

2 of 13

Yes

Xtro\arhgap20

1 of 13

Xtro\capn7

1 of 13

Xtro\capn8.2

1 of 13

Yes

Xtro\capn8.6

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC101731595

1 of 13

Yes

Xtro\mbip

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (30)

Drer\capn9

13 of 14

Yes

Drer\capn1a

11 of 14

Yes

Drer\capn1b

11 of 14

Yes

Drer\capn1

11 of 14

Yes

Drer\capn2b

11 of 14

Yes

Drer\capn2l

11 of 14

Yes

Drer\capn3a

11 of 14

Yes

Drer\capn3b

11 of 14

Yes

Drer\capn2a

10 of 14

Yes

Drer\capn8

10 of 14

Yes

Drer\si:dkeyp-50d11.2

10 of 14

Yes

Drer\zgc:136872

8 of 14

Yes

Drer\capn12

7 of 14

Drer\si:ch211-202f3.3

7 of 14

Yes

Drer\si:ch211-202f3.4

6 of 14

Yes

Drer\zgc:162184

6 of 14

Yes

Drer\zgc:85932

5 of 14

Drer\capn5a

4 of 14

Yes

Drer\capn5b

4 of 14

Yes

Drer\capn10

3 of 14

Yes

Drer\capn15

3 of 14

Drer\si:dkey-19b23.12

3 of 14

Yes

Drer\adgb

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (24)

Cele\clp-1

12 of 14

Yes

Cele\clp-7

10 of 14

Yes

Cele\clp-6

9 of 14

Yes

Cele\clp-3

8 of 14

Yes

Cele\clp-4

7 of 14

Yes

Cele\tra-3

5 of 14

Yes

Cele\clpr-4

4 of 14

Yes

Cele\clp-8

3 of 14

Yes

Cele\clp-10

3 of 14

Cele\clpr-1

3 of 14

Yes

Cele\clpr-2

3 of 14

Yes

Cele\clpr-3

3 of 14

Yes

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (16)

Agam\AgaP_AGAP012068

12 of 12

Yes

Agam\AgaP_AGAP012066

7 of 12

Yes

Agam\AgaP_AGAP012069

5 of 12

Yes

Agam\AgaP_AGAP012907

5 of 12

Yes

Agam\AgaP_AGAP010395

4 of 12

Agam\AgaP_AGAP012067

4 of 12

Yes

Agam\AgaP_AGAP012264

3 of 12

Yes

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (41)

Atha\DEK1

8 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Yes

Atha\AT3G03000

1 of 13

Atha\AT3G10190

1 of 13

Yes

Atha\AT3G25600

1 of 13

Atha\AT3G59440

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\TCH2

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (3)

Scer\PEF1

2 of 13

Yes

Scer\RIM13

2 of 13

Yes

Scer\CDC31

1 of 13

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CalpA. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (10)

CalpB

12 of 13

CalpC

8 of 13

sol

5 of 13

Alg-2

2 of 13

Pef

2 of 13

azot

1 of 13

Cam

1 of 13

CG13526

1 of 13

SCaMC

1 of 13

Xkr

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

CalpA^KK104532

model of Huntington's disease

CEC with Hsap\HTT^GMR.Q120

(Menzies et al., 2015)

CalpA^JF03391

model of intellectual disability

CEA

(Haddadi and Ataei, 2022)

model of obesity

CEA

(Agrawal et al., 2021)

Potential Models Based on Orthology ( 4 )

Human Ortholog

Disease

Evidence

References

CAPN3; calpain 3

model of autosomal recessive limb-girdle muscular dystrophy type 2A

IEA

(FlyBase, 2019-)

CAPN1; calpain 1

model of hereditary spastic paraplegia 76

IEA

(FlyBase, 2019-)

CAPN10; calpain 10

model of type 2 diabetes mellitus

IEA

(FlyBase, 2019-)

CAPN5; calpain 5

model of neovascular inflammatory vitreoretinopathy

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 7 )

Allele

Disease

Interaction

References

CalpA^e01545

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Reinecke et al., 2011)

CalpA^GD12275

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Miller et al., 2010)

CalpA^KG05080

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Reinecke et al., 2011)

CalpA^KK104532

ameliorates Huntington's disease

modeled by Hsap\HTT^GMR.Q120

(Menzies et al., 2015)

modeled by Hsap\HTT^{Q46.ex1p.UAS.EGFP}

(Menzies et al., 2015)

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{Q48.UAS.Tag:MYC,Tag:FLAG}

(Menzies et al., 2015)

ameliorates Alzheimer's disease

modeled by Hsap\MAPT^UAS.cWa

(Menzies et al., 2015)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Park et al., 2020)

CalpA^JF03391

exacerbates amyotrophic lateral sclerosis type 10

modeled by TBPH^{UAS.Tag:FLAG.Tag:HA}

(Park et al., 2020)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Park et al., 2020)

CalpA^NIG.18152R

ameliorates Huntington's disease

modeled by Hsap\HTT^{Q46.ex1p.UAS.EGFP}

(Menzies et al., 2015)

CalpA^NIG.7563R

ameliorates Huntington's disease

modeled by Hsap\HTT^{Q46.ex1p.UAS.EGFP}

(Menzies et al., 2015)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

13 of 14

12 of 14

autosomal recessive limb-girdle muscular dystrophy type 2A

12 of 14

11 of 14

SPASTIC PARAPLEGIA 76, AUTOSOMAL RECESSIVE; SPG76

hereditary spastic paraplegia 76

11 of 14

11 of 14

6 of 14

6 of 14

6 of 14

4 of 14

VITREORETINOPATHY, NEOVASCULAR INFLAMMATORY; VRNI

neovascular inflammatory vitreoretinopathy

4 of 14

4 of 14

TYPE 2 DIABETES MELLITUS 1; T2D1

type 2 diabetes mellitus

CAPN15; calpain 15

3 of 14

603267

OCULOGASTROINTESTINAL NEURODEVELOPMENTAL SYNDROME; OGIN

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CalpA - CalpB

anti tag coimmunoprecipitation, anti tag western blot, western blot, anti bait coimmunoprecipitation

(Metwally et al., 2019)

CalpA - CalpC

anti bait coimmunoprecipitation, anti tag western blot

(Metwally et al., 2019)

CalpA - GluRIIA

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation

(Metwally et al., 2019)

CalpA - cact

anti tag coimmunoprecipitation, anti tag western blot

(Fontenele et al., 2013)

CalpA - sol

anti bait coimmunoprecipitation, anti tag western blot

(Metwally et al., 2019)

CalpA - ttm50

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, bio-layer interferometry, predetermined participant, pull down

(Metwally et al., 2021)

CalpA - yki

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti tag western blot

(Zhai et al., 2024)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

CalpA

suppressible

ttm50

(Metwally et al., 2021)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

pbl

enhanceable

CalpA

(Gregory et al., 2007)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62895

DroID - A comprehensive database of gene and protein interactions.

FBgn0012051

MIST (protein-protein) - An integrated Molecular Interaction Database

37232

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Neutrophil degranulation

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-89

Cytogenetic map

56D5-56D5

Sequence location

2R:19,425,113..19,430,564 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

56D5-56D5

Limits computationally determined from genome sequence between P{lacW}hts^k06121 and P{lacW}l(2)k00705^k00705&P{lacW}mei-W68^k05603

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

56C-56D

(determined by in situ hybridisation)

(Theopold et al., 1995)

50D-50E

(determined by in situ hybridisation)

(Emori and Saigo, 1994)

Experimentally Determined Recombination Data

Location

2-89

(FlyBase, 2016)

2-94.7

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

29455

y¹ v¹; P{TRiP.JF03391}attP2

65441

w¹¹¹⁸; PBac{IT.GAL4}CalpA^1105-G4

78997

y¹ w^*; TI{CRIMIC.GT14}CalpA^CR99905

83262

y¹ w^*; TI{CRIMIC.TG4.0}CalpA^{CR01313-TG4.0}

Kyoto

201527

y¹ w^67c23; P{GSV6}CalpA^GS9103/SM1

203630

y¹ w^67c23; P{GSV6}CalpA^GS11846/SM1

NIG-Fly

7563R-3

P{NIG.7563R}3

HMJ23555

y¹ v¹; P{TRiP.HMJ23555}attP40/CyO

2RG-0850

y² VhaAC39-1^cho-1 v¹; P{U6-CalpA.NIG.sgRNA}attP40/CyO

VDRC

v101294

P{KK104532}VIE-260B

v35261

w¹¹¹⁸; P{GD12275}v35261

v35262

w¹¹¹⁸; P{GD12275}v35262

Genomic Clones (27)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (84)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD22862

Other clones

DPiM_LD22862

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0012051

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0012051

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Metwally et al., 2021, Theopold et al., 1995, Emori and Saigo, 1994)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

CalpA is epistatic to sog.

(Fontenele et al., 2009)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

CalpA has been cloned and sequenced, multiple transcripts derive from a single CalpA gene. CalpA is expressed in a highly specific pattern in a few cells in the central nervous system, in blood cells and in scattered cells in the midgut.

(Theopold et al., 1995)

Localisation of CalpA in the developing embryo indicates CalpA is involved in the dynamic changes of the embryonic cytoskeleton, especially actin-related structures, during early embryogenesis prior to cellularisation.

(Emori and Saigo, 1994)

Relationship to Other Genes

Source for database merge of

Source for merge of: CalpA CG18152

(FlyBase, 1996-)

Additional comments

It is likely that the "CalpII" activity (identified and studied in FBrf0047562) corresponds to the "Canp" protein (94 kDa, purified and studied in FBrf0055463), since both are activated by high Ca[2+] concentrations, and that this protein is encoded by the "CalpA" gene since this is the only Calpain gene with a protein product in the range 90-97 kDa.

(Marygold, 2025.1.31)

"CalpI" (an activity identified and studied in FBrf0047562), "CalpII" (also an activity identified and studied in FBrf0047562) and "Canp" (a protein purified and studied in FBrf0055463) are unlikely to be the same as "CalpA", since "Canp", "CalpI" and "CalpII" proteins are purified from the cytosol and "CalpA" is not detected in the cytosol with an anti-"CalpA" antibody.

(Pinter, 1998.7.1)

Source for merge of CalpA CG18152 was a shared cDNA ( date:010720 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: CalpA CG7563

(Milshina, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

CG18152

(Menzies et al., 2015)

CG7563

(National Institute of Genetics Fly Stocks, 2023-, National Institute of Genetics Fly Stocks, 2022-, Menzies et al., 2015, Christis and Munro, 2012, Cammarato et al., 2011, Keleman et al., 2009.8.5, Perkins et al., 2009, Stempfl et al., 2002)

EST C

(Palmer et al., 1999)

calpA

(Agrawal et al., 2021, Kiragasi et al., 2020, Schloop et al., 2020, Metwally et al., 2019, Kanamori et al., 2013)

Name Synonyms

Calpain

(Pirooznia et al., 2012)

Calpain A

(Gistelinck et al., 2012, Paik et al., 2012, Fontenele et al., 2009, Araujo et al., 2008, Friedrich, 2004)

Calpain lacking calmodulin-like domain

(Hultmark, 1994.12.5)

Calpain-A

(Peiris et al., 2018, Nakayama et al., 2014, Cammarato et al., 2011, Marcu et al., 2011, Gim et al., 2001)

calpain

(Busto et al., 2017, Jaspers et al., 2012, Gregory et al., 2007, Emori, 1994.3.30, Aszodi et al., 1991)

calpain A

(Metwally et al., 2019)

calpainA

(Kiragasi et al., 2020, Reinecke et al., 2011)

Secondary FlyBase IDs

FBgn0034444

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 63 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37232

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q11002

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q11002

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q11002

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0012051

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37232/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0012051

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0012051

FlyMine - An integrated database for Drosophila genomics

FBgn0012051

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG7563

MARRVEL_MODEL - MARRVEL (model organism gene)

37232

MEROPS - An information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit them.

C02.950

Linkouts

BioGRID - A database of protein and genetic interactions.

62895

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0012051

DroID - A comprehensive database of gene and protein interactions.

FBgn0012051

DRSC - Results frm RNAi screens

FBgn0012051

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

CalpA_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0012051

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0012051

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7563

Flygut - An atlas of the Drosophila adult midgut

FBgn0012051

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0012051

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032612

MIST (protein-protein) - An integrated Molecular Interaction Database

37232

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Neutrophil degranulation

References (111)

The following external sites may use different assemblies or annotations than FlyBase.

-0.81

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