FlyBase Gene Report: Dmel\Nap1

General Information

Symbol

Dmel\Nap1

Species

D. melanogaster

Name

Nucleosome assembly protein 1

Annotation Symbol

CG5330

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015268

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

Nucleosome assembly protein 1 (Nap1) encodes a core histone chaperone involved in histone nuclear transfer and chromatin assembly. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

NAP-1, dNAP-1, dNap1

Key Links

Genomic Location

Cytogenetic map

60A9-60A9

Sequence location

2R:23,905,175..23,907,294 [-]

Recombination map

2-105

RefSeq locus

NT_033778 REGION:23905175..23907294

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables histone binding

inferred from direct assay

(Emelyanov et al., 2014, Kimura, 2013, Ito et al., 1996)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables chromatin binding

inferred from biological aspect of ancestor with PANTHER:PTN000221934

(GO Reference Genome Project, 2011-)

enables histone binding

traceable author statement

(Ito et al., 1997)

inferred from biological aspect of ancestor with PANTHER:PTN000221934

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in chromatin remodeling

inferred from direct assay

(Emelyanov et al., 2014)

involved_in DNA replication-dependent chromatin assembly

inferred from direct assay

(Ito et al., 1997)

involved_in nucleosome assembly

inferred from direct assay

(Kimura, 2013, Ito et al., 1996)

involved_in sperm DNA condensation

inferred from mutant phenotype

(Doyen et al., 2015)

involved_in sperm DNA decondensation

inferred from direct assay

(Emelyanov et al., 2014)

involved_in telomere maintenance

inferred from mutant phenotype

(López-Panadès and Casacuberta, 2016)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in nucleosome assembly

inferred from electronic annotation with InterPro:IPR002164

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000221935

(GO Reference Genome Project, 2011-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Emelyanov et al., 2014, Ito et al., 1996)

located_in mitochondrion

inferred from direct assay

(Emelyanov et al., 2014)

located_in nucleus

inferred from direct assay

(Emelyanov et al., 2014, Ito et al., 1996)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in chromatin

inferred from biological aspect of ancestor with PANTHER:PTN000221934

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000221934

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred from electronic annotation with InterPro:IPR002164

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Nucleosome assembly protein 1 (Nap1) encodes a core histone chaperone involved in histone nuclear transfer and chromatin assembly. [Date last reviewed: 2019-03-14]

Summary (Interactive Fly)

Histone chaperone - chromatin assembly - core histone chaperone involved in histone nuclear transfer and chromatin assembly - regulates the transcription of the HeT-A retrotransposon

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nap1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W1G7)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nap1-RA

FBtr0072219

NM_057780

1361

370

Nap1-RB

FBtr0305989

NM_001259556

1393

370

Nap1-RC

FBtr0305990

NM_001259557

1376

370

Additional Transcript Data and Comments

Reported size (kB)

1.5 (northern blot)

(Ito et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Nap1-PA

FBpp0072128

42.8

370

4.40

FBpp0297131

42.8

370

4.40

FBpp0297132

42.8

370

4.40

Q9W1G7

NP_001246486

AFH08239

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

370 aa isoforms: Nap1-PA, Nap1-PB, Nap1-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

370 (aa); 56 (kD observed)

(Ito et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nap1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.44

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

embryonic central brain neuron

(Fisher et al., 2012)

fat body/gonad primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic anal pad

(Fisher et al., 2012)

embryonic brain

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

larval visceral muscle cell

(Fisher et al., 2012)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | male

organism

Comment: male-specific

(Doyen et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

(Ito et al., 1996)

(Doyen et al., 2015)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Ito et al., 1999, Ito et al., 1996)

adult stage | male

organism

(Doyen et al., 2015)

Additional Descriptive Data

A broad distribution of Nap1 protein is observed in the testis during spermatid development with strong staining of the flagella.

(Doyen et al., 2015)

Nap1 protein is present at high levels throughout development except in adult males where it is present at lower levels.

(Ito et al., 1999)

Nlp and Nap1 proteins are both expressed at highest levels in early embryos, and at reduced but significant levels in larval through adult stages.

(Ito et al., 1996)

In early Drosophila embryos, Nap1 protein is present in the nucleus and cytoplasm during S phase, and is mainly cytoplasmic during G2-phase.

(Ito et al., 1996)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Emelyanov et al., 2014, Ito et al., 1996)

located_in mitochondrion

inferred from direct assay

(Emelyanov et al., 2014)

located_in nucleus

inferred from direct assay

(Emelyanov et al., 2014, Ito et al., 1996)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

Expression Deduced from Reporters

Reporter: P{ST.lexA::HG}Nap1^JPC-2

Stage

Tissue/Position (including subcellular localization)

Reference

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Nap1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Nap1

View exonic expression by tissue for Dmel\Nap1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015268

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37798/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015268

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015268

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015268

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5330

Flygut - An atlas of the Drosophila adult midgut

FBgn0015268

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Nap1

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Nap1

Transgenic constructs containing regulatory region of Nap1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6080

(Ryder, 2004.4.26)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

partially lethal, with Scer\GAL4^tin.CΔ4

Nap1^GD11609

partially lethal - majority die

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Nap1^GD11609

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Nap1^GD11609

Sterility

Allele

fertile

Nap1^EY08596

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Nap1^GD11609

some die during pupal stage, with Scer\GAL4^pnr-MD237

Nap1^GD11609

suppressor of variegation | dominant, with In(1)w^m4

visible, with Scer\GAL4^pnr-MD237

Nap1^GD11609

wild-type

Nap1^KG03959

Phenotype manifest in

Allele

eye, with Scer\GAL4^GMR.PF

Nap1^GD11609

investment cone

Nap1^KO1

mesonotum, with Scer\GAL4^pnr-MD237

Nap1^GD11609

trichogen cell, with Scer\GAL4^pnr-MD237

Nap1^GD11609

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (21)

Hsap\NAP1L1

13 of 14

Yes

Hsap\NAP1L4

11 of 14

Yes

Hsap\NAP1L2

7 of 14

Yes

Hsap\NAP1L3

6 of 14

Yes

Hsap\NAP1L5

4 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Hsap\SET

1 of 14

Hsap\TSPY9P

1 of 14

Hsap\TSPY26P

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (16)

Rnor\Nap1l1

13 of 14

Yes

Rnor\RGD1560617

11 of 14

Yes

Rnor\Nap1l4

10 of 14

Yes

Rnor\Nap1l3

6 of 14

Yes

Rnor\Nap1l5

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\RGD1563307

1 of 14

Mus musculus (laboratory mouse) (11)

Mmus\Nap1l1

12 of 14

Yes

Mmus\Nap1l4

10 of 14

Yes

Mmus\Nap1l2

7 of 14

Yes

Mmus\Nap1l3

6 of 14

Yes

Mmus\Nap1l5

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Xenopus tropicalis (Western clawed frog) (5)

Xtro\nap1l1

11 of 13

Yes

Xtro\nap1l4

6 of 13

Yes

Xtro\acer3

1 of 13

Xtro\set

1 of 13

Xtro\tspyl2

1 of 13

Danio rerio (Zebrafish) (6)

Drer\nap1l1

12 of 14

Yes

Drer\nap1l4a

11 of 14

Yes

Drer\nap1l4b

11 of 14

Yes

Drer\tspy

2 of 14

Drer\seta

1 of 14

Drer\setb

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\nap-1

13 of 14

Yes

Cele\spr-2

1 of 14

Anopheles gambiae (African malaria mosquito) (3)

Agam\AgaP_AGAP001928

12 of 12

Yes

Agam\AgaP_AGAP009639

4 of 12

Yes

Agam\AgaP_AGAP003598

1 of 12

Arabidopsis thaliana (thale-cress) (6)

Atha\NAP1;1

13 of 13

Yes

Atha\NAP1;2

13 of 13

Yes

Atha\NAP1;3

13 of 13

Yes

Atha\NAP1;4

11 of 13

Yes

Atha\NRP1

1 of 13

Atha\NRP2

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\NAP1

13 of 13

Yes

Scer\VPS75

1 of 13

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\nap1

12 of 12

Yes

Spom\nap2

11 of 12

Yes

Spom\SPBC36B7.08c

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Nap1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (3)

CG3708

10 of 13

mil

7 of 13

Set

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Nap1^KK101965

exacerbates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\poly-GR^50.UAS.EGFP

(Lee et al., 2016)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NAP1L1; nucleosome assembly protein 1 like 1

13 of 14

164060

NAP1L4; nucleosome assembly protein 1 like 4

11 of 14

601651

NAP1L2; nucleosome assembly protein 1 like 2

7 of 14

300026

NAP1L3; nucleosome assembly protein 1 like 3

6 of 14

300117

NAP1L5; nucleosome assembly protein 1 like 5

4 of 14

612203

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Nap1 - smg

anti bait coimmunoprecipitation, quantitative reverse transcription pcr

(Chen et al., 2014)

protein-protein

Physical Interaction

Assay

References

Nap1 - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Nap1 - CG15356

anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot

(Moshkin et al., 2009)

Nap1 - CG3815

anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot

(Moshkin et al., 2009)

Nap1 - Caf1-55

anti bait coimmunoprecipitation, western blot

(Stephens et al., 2006)

Nap1 - E(bx)

chromatography technology, western blot, anti bait coimmunoprecipitation

(Stephens et al., 2006)

Nap1 - HDAC1

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Moshkin et al., 2009)

Nap1 - HSPC300

pull down, molecular weight estimation by staining

(Chia et al., 2014)

Nap1 - HeT-A\gag

anti tag coimmunoprecipitation, peptide massfingerprinting, anti bait coimmunoprecipitation, western blot

(López-Panadès and Casacuberta, 2016)

Nap1 - His1

anti bait coimmunoprecipitation, western blot

(Yoneda et al., 2022)

Nap1 - His2A

anti bait coimmunoprecipitation, western blot, cosedimentation through density gradient, molecular weight estimation by staining, cosedimentation

(Emelyanov et al., 2014, Ito et al., 1996, Ito et al., 1996, Bulger et al., 1995)

Nap1 - His2B

anti bait coimmunoprecipitation, western blot, cosedimentation, molecular weight estimation by staining, cosedimentation through density gradient

(Emelyanov et al., 2014, Ito et al., 1996, Ito et al., 1996, Bulger et al., 1995)

Nap1 - His3

tandem affinity purification, western blot, cosedimentation, molecular weight estimation by staining, Identification by mass spectrometry, cosedimentation through density gradient

(Fromental-Ramain et al., 2017, Emelyanov et al., 2014, Ito et al., 1996, Ito et al., 1996, Bulger et al., 1995)

Nap1 - His3.3A

tandem affinity purification, western blot, Identification by mass spectrometry

(Fromental-Ramain et al., 2017)

Nap1 - His3.3B

tandem affinity purification, Identification by mass spectrometry, western blot

(Fromental-Ramain et al., 2017)

Nap1 - His4

cosedimentation through density gradient, molecular weight estimation by staining, cosedimentation

(Emelyanov et al., 2014, Ito et al., 1996, Ito et al., 1996, Bulger et al., 1995)

Nap1 - Iswi

anti bait coimmunoprecipitation, western blot

(Stephens et al., 2006)

Nap1 - Kdm5

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot

(Moshkin et al., 2009)

Nap1 - MRG15

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Moshkin et al., 2009)

Nap1 - Mettl14

anti tag coimmunoprecipitation, anti tag western blot

(Tang et al., 2021)

Nap1 - Mst77F

anti bait coimmunoprecipitation, Identification by mass spectrometry, molecular weight estimation by staining

(Emelyanov et al., 2023)

Nap1 - Nurf-38

anti bait coimmunoprecipitation, western blot

(Stephens et al., 2006)

Nap1 - ProtA

anti tag coimmunoprecipitation, peptide massfingerprinting, pull down, anti tag western blot

(Emelyanov and Fyodorov, 2016, Emelyanov et al., 2014)

Nap1 - Rheb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Nap1 - SA1

anti bait coimmunoprecipitation, western blot, pull down, autoradiography

(Moshkin et al., 2013)

Nap1 - SMC1

pull down, autoradiography, anti bait coimmunoprecipitation, western blot

(Moshkin et al., 2013)

Nap1 - SMC3

pull down, autoradiography

(Moshkin et al., 2013)

Nap1 - Sfmbt

anti tag coimmunoprecipitation, peptide massfingerprinting, western blot

(Alfieri et al., 2013)

Nap1 - Sin3A

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Moshkin et al., 2009)

Nap1 - Sirt1

anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot

(Horvath et al., 2016)

Nap1 - Smn

anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot

(Matera et al., 2024)

Nap1 - Su(H)

anti bait coimmunoprecipitation, western blot

(Moshkin et al., 2009)

Nap1 - Su(var)2-HP2

anti bait coimmunoprecipitation, western blot, chromatography technology

(Stephens et al., 2006)

Nap1 - Su(var)205

chromatography technology, western blot

(Stephens et al., 2006)

Nap1 - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Nap1 - dhd

pull down, anti tag western blot

(Emelyanov and Fyodorov, 2016)

Nap1 - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Nap1 - mts

anti bait coimmunoprecipitation, western blot

(Moshkin et al., 2013)

Nap1 - polo

pull down, autoradiography

(Moshkin et al., 2013)

Nap1 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Nap1 - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Nap1 - vtd

pull down, autoradiography, anti bait coimmunoprecipitation, western blot

(Moshkin et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

63390

DroID - A comprehensive database of gene and protein interactions.

FBgn0015268

MIST (protein-protein) - An integrated Molecular Interaction Database

37798

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-105

Cytogenetic map

60A9-60A9

Sequence location

2R:23,905,175..23,907,294 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

60A9-60A9

Limits computationally determined from genome sequence between P{lacW}ken^k11035&P{EP}CG17658^EP730 and P{EP}Tal^EP489&P{EP}CG3065^EP316

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

60A5-60A6

(determined by in situ hybridisation)

(Ito et al., 1996)

Experimentally Determined Recombination Data

Location

2-105

(FlyBase, 2016)

2-109.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

13355

y¹ w^67c23; P{SUPor-P}Nap1^KG03959

35445

y¹ sc^* v¹ sev²¹; P{TRiP.GL00370}attP2

65936

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06207}attP2

38257

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01701}attP40

8537

w¹¹¹⁸; TI{TI}Nap1^KO1/T(2;3)SM6b-TM6B, ap^RK Tb¹

8538

w¹¹¹⁸; TI{TI}Nap1^KO2/T(2;3)SM6b-TM6B, ap^RK Tb¹

Kyoto

113860

y^* w^*; P{GawB}Nap1^NP6207 / CyO, P{UAS-lacZ.UW14}UW14

NIG-Fly

5330R-1

P{NIG.5330R}1

GL00370

y¹ sc^* v¹ sev²¹; P{TRiP.GL00370}attP2

2RG-1091

y² VhaAC39-1^cho-1 v¹; P{U6-Nap1.NIG.sgRNA}attP40/CyO

VDRC

v109299

P{KK101965}VIE-260B

v27393

w¹¹¹⁸; P{GD11609}v27393

More stocks available...

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (257)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD21576

Other clones

MIP15604

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015268

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015268

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Yoneda et al., 2022, Kal et al., 2000)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Acf1 together with a core histone chaperone (such as Caf1 or Nap1) is sufficient for ATP-dependent formation of periodic nucleosome arrays.

(Ito et al., 1997)

Nap1 acts as a core histone-binding protein (shuttle) which delivers histones from the cytoplasm to the chromatin assembly machinery in the nucleus. The p56 component of Caf4 (chromatin assembly factor 4).

(Ito et al., 1996)

Isolated as a protein that copurified with the p127 product of l(2)gl.

(Neumann et al., 1996)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Nap1 CG5330

(Zhang, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG5330

(National Institute of Genetics Fly Stocks, 2022-, Kolkhof et al., 2017, Boltengagen et al., 2016, Çiçek et al., 2016, Hosono et al., 2015, Bridon et al., 2012, Ni et al., 2011.7.19, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009)

NAP1

(Emelyanov et al., 2023, Okimune et al., 2021, Emelyanov and Fyodorov, 2016, Doyen et al., 2015, Vlijm et al., 2015, Moshkin et al., 2013, Torigoe et al., 2013, Moshkin et al., 2009, Tanaka et al., 2007, Brasaemle and Hansen, 2006, Polo and Almouzni, 2006, Lusser et al., 2005, Verreault, 2000, Li et al., 1999, Li et al., 1999, Grunstein, 1997)

Nap-1

(López-Panadès and Casacuberta, 2016, Stephens et al., 2006, Lankenau et al., 2003, McNairn and Gilbert, 2003)

Nap1

(Dou et al., 2023, Yheskel et al., 2023, Ding et al., 2022, Gorsi et al., 2022, Kang et al., 2022, Hirschhäuser et al., 2021, Meyer-Nava et al., 2020, Okimune et al., 2020, Beckmann et al., 2019, Meltzer et al., 2019, Tsui et al., 2018, Boltengagen et al., 2016, Horvath et al., 2016, Kockel et al., 2016, Lee et al., 2016, Dent et al., 2015, Fei et al., 2015, Kwong et al., 2015, Spratford and Kumar, 2015, Ashwal-Fluss et al., 2014, Chen et al., 2014, Chen et al., 2014, Luck et al., 2014, Alfieri et al., 2013, Kwon et al., 2013, Moshkin et al., 2013, Zhu et al., 2013, Bridon et al., 2012, Hazelett et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Abruzzi et al., 2011, Friedman et al., 2011, Lott et al., 2011, Blanco et al., 2010, Müller et al., 2010, Tan et al., 2009, Hutter et al., 2008, Stuart et al., 2007)

dCAF-4

(Bulger et al., 1995)

dNAP-1

(Endo et al., 2021, Sawatsubashi et al., 2010, Bao et al., 2004, Asahara et al., 2002, Georges et al., 2002, Levenstein and Kadonaga, 2002, Huang and Kadonaga, 2001, Nakagawa et al., 2001, Ito et al., 1999, Ito et al., 1997, Ito et al., 1996, Ito et al., 1996)

dNAP1

(Jiang et al., 2000, Kal et al., 2000)

dNap1

(Chia et al., 2014, Adams and Kamakaka, 1999)

nap-1

(Lee et al., 2017, López-Panadès and Casacuberta, 2016)

nap1

(Kimura, 2013, Moshkin et al., 2013)

p56

(Bulger et al., 1995)

p56/dCAF-4

(Ito et al., 1999)

Name Synonyms

Nucleosome assembly protein 1

nucleosome assembly protein 1

(Bridon et al., 2012)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

37798

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W1G7

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W1G7

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015268

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

37798/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015268

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015268

FlyMine - An integrated database for Drosophila genomics

FBgn0015268

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5330

MARRVEL_MODEL - MARRVEL (model organism gene)

37798

Linkouts

BioGRID - A database of protein and genetic interactions.

63390

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015268

DroID - A comprehensive database of gene and protein interactions.

FBgn0015268

DRSC - Results frm RNAi screens

FBgn0015268

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015268

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015268

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5330

Flygut - An atlas of the Drosophila adult midgut

FBgn0015268

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032798

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

37798

References (154)

The following external sites may use different assemblies or annotations than FlyBase.

-0.44

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