FlyBase Gene Report: Dmel\Mcm6

General Information

Symbol

Dmel\Mcm6

Species

D. melanogaster

Name

Minichromosome maintenance 6

Annotation Symbol

CG4039

Feature Type

protein_coding_gene

FlyBase ID

FBgn0025815

Gene Model Status

Current

Stock Availability

14 publicly available

Enzyme Name (EC)

DNA helicase (3.6.4.12)

DNA 3'-5' helicase (5.6.2.4)

Gene Summary

Minichromosome maintenance 6 (Mcm6) encodes a subunit of the hetero-hexameric mini-chromosome maintenance (MCM) Complex, which is the eukaryotic replicative DNA helicase. MCM complex requires association with CDC45L and the GINS complex for full helicase activity. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

fs(1)K1214, DmMCM6

Key Links

Genomic Location

Cytogenetic map

6C4-6C5

Sequence location

X:6,674,387..6,677,176 [+]

Recombination map

1-18

RefSeq locus

NC_004354 REGION:6674387..6677176

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (7 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

contributes_to 3'-5' DNA helicase activity

inferred from direct assay

(Moyer et al., 2006)

Terms Based on Predictions or Assertions (6 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008049, InterPro:IPR018525, InterPro:IPR031327

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from sequence or structural similarity with UniProtKB:P97311

(Gene Ontology Curators, 2002-)

enables DNA binding

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008049, InterPro:IPR018525, InterPro:IPR031327

(InterPro Project Members, 2004-)

enables DNA helicase activity

inferred from electronic annotation with InterPro:IPR008049

(InterPro Project Members, 2004-)

contributes_to single-stranded 3'-5' DNA helicase activity

inferred from biological aspect of ancestor with PANTHER:PTN000180096

(GO Reference Genome Project, 2011-)

contributes_to single-stranded DNA binding

inferred from sequence or structural similarity with UniProtKB:P97311

(Gene Ontology Curators, 2002-)

enables single-stranded DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000179711

(GO Reference Genome Project, 2011-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in DNA replication

inferred from mutant phenotype

(Schwed et al., 2002)

involved_in eggshell chorion gene amplification

inferred from mutant phenotype

(Claycomb et al., 2004)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in DNA replication initiation

inferred from electronic annotation with InterPro:IPR008049

(InterPro Project Members, 2004-)

involved_in DNA replication

inferred from electronic annotation with InterPro:IPR018525

(InterPro Project Members, 2004-)

involved_in double-strand break repair via break-induced replication

inferred from biological aspect of ancestor with PANTHER:PTN000180096

(GO Reference Genome Project, 2011-)

involved_in mitotic DNA replication

inferred from biological aspect of ancestor with PANTHER:PTN000180096

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

part_of CMG complex

inferred from direct assay

(Ilves et al., 2010, Moyer et al., 2006)

part_of MCM complex

inferred from direct assay

(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)

located_in nucleus

inferred from direct assay

(Schwed et al., 2002)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of MCM complex

inferred from electronic annotation with InterPro:IPR008049

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000179711

(GO Reference Genome Project, 2011-)

located_in nucleus

inferred from electronic annotation with InterPro:IPR008049

(InterPro Project Members, 2004-)

Gene Group (FlyBase)

MCM2-7 COMPLEX

CMG COMPLEX

MCM FAMILY HELICASES

Protein Family (UniProt)

Belongs to the MCM family. (Q9V461)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065: 3'-5' DNA helicase activity

Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction (5.6.2.4)

DNA helicase activity

ATP + H2O = ADP + phosphate + H(+) (3.6.4.12)

Summaries

Gene Snapshot

Gene Group (FlyBase)

MCM2-7 COMPLEX -: The heterohexameric MCM2-7 complex is the helicase motor of the CMG (Cdc45-MCM-GINS) replicative helicase of eukaryotes. The six MCM subunits are related to one another and are members of the AAA+ (ATPases associated with diverse cellular activities) superfamily. Hydrolysis of ATP by the individual MCM subunits catalyses the conformational changes that drive unwinding of the DNA duplex. However, the eukaryotic MCM complex alone has no or little helicase activity, requiring the other components of the CMG complex (Cdc45 and the GINS complex) for activation. (Adapted from PMID:23412083.)
CMG COMPLEX -: The CMG (Cdc45-MCM-GINS) complex is the eukaryotic replicative helicase, the enzyme that unwinds double-stranded DNA at replication forks. It is a macromolecular assembly of three replication factors: the Cdc45 protein, and the MCM and GINS complexes. All three components are essential for CMG function, with the MCM2-7 complex functioning as the molecular motor that harnesses the energy of ATP hydrolysis to catalyse strand separation. (Adapted from PMID:23412083.)
MCM FAMILY HELICASES -: The MCM (minichromosome maintenance) family comprise DNA helicases sharing a common 'MCM domain'. MCM2-7 form a heterohexameric complex that is essential for initiation of DNA replication and also acts during elongation as the replicative helicase. MCM8 and MCM9 are not part of this complex - they have roles in replication elongation, meiotic recombination and DNA repair. MCM8 and 9 arose early during eukaryotic evolution, though one or both have been lost in multiple lineages: in mammals, MCM8 and MCM9 form a functional heterodimeric complex; D. melanogaster and related species possess MCM8 only; while other species (e.g. yeast, C. elegans) have neither. (Adapted from PMID:23412083, PMID:30743181 and FBrf0188222.)

Protein Function (UniProtKB)

Acts as a component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity Required for DNA replication and cell proliferation. Required for mitotic cycles, endocycles, and the special S phase associated with the amplification of chorion genes; has a role in origin unwinding or fork elongation at chorion loci.

(UniProt, Q9V461)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

fs(1)K1214

Viability and fecundity of homozygous females reduced; sterility virtually complete, but a few progeny escape. Dorsal appendages of chorion short and thin. All major chorion proteins underproduced owing to a defect in amplification of Cp genes on both the X and chromosome 3 (Orr, Komitopoulou, and Kafatos, 1984, Proc. Nat. Acad. Sci. USA 81: 3773-77). Mosaic analysis indicates somatic function of gene (Perrimon and Gans, 1983, Dev. Biol. 100: 365-73).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mcm6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V461)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

Comments on Gene Model

Gene model reviewed during 5.50

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mcm6-RA

FBtr0070952

NM_078510

2790

817

Additional Transcript Data and Comments

Reported size (kB)

3.0 (northern blot)

(Ohno et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Mcm6-PA

FBpp0070913

92.4

817

5.01

Q9V461

NP_511065

AAF46184

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

817 (aa)

(Feger, 1999, Ohno et al., 1998)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). The heterodimers of Mcm4/Mcm6 and Mcm3/Mcm5 interact with Mcm2 and Mcm7.

(UniProt, Q9V461)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V461

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcm6 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.22

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 12

presumptive embryonic/larval central nervous system

(Feger, 1999)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 9

Comment: reference states 0-4 hr AEL

(Ohno et al., 1998)

adult stage | female

(Ohno et al., 1998)

unfertilized egg stage

unfertilized egg

(Ohno et al., 1998)

Additional Descriptive Data

Mcm6 transcript expression pattern resembles that of DNA replication. Mcm6 transcript is detected at high levels in the central nervous system, and at low levels in the gut of the stage 11-12 embryo.

(Feger, 1999)

Mcm6 transcript levels are high in unfertilized eggs, slightly higher in 2-4 hour embryos, and are low after 4 hours of embryogenesis. Levels are low in other developmental stages, with the exception of adult females.

(Ohno et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of CMG complex

inferred from direct assay

(Ilves et al., 2010, Moyer et al., 2006)

part_of MCM complex

inferred from direct assay

(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)

located_in nucleus

inferred from direct assay

(Schwed et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mcm6 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mcm6

View exonic expression by tissue for Dmel\Mcm6

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31603/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025815

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025815

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025815

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4039

Flygut - An atlas of the Drosophila adult midgut

FBgn0025815

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mcm6

Transgenic constructs containing regulatory region of Mcm6

Aberrations (Deficiencies and Duplications) ( 10 )

Inferred from experimentation ( 10 )

Gene disrupted in

Df(1)6C-190

(Schwed et al., 2002)

Df(1)6C-310

(Schwed et al., 2002)

Df(1)BSC535

(Christensen et al., 2008.6.11)

Gene not disrupted in

Df(1)5D

(Schwed et al., 2002)

Df(1)6C-25

(Schwed et al., 2002)

Df(1)6C-40

(Schwed et al., 2002)

Df(1)6C-51

(Schwed et al., 2002)

Df(1)dx81

(Cook, 2016.2.8)

Df(1)JF5

(Schwed et al., 2002)

Df(1)N73

(Schwed et al., 2002)

Inferred from location ( 25 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Mcm6²)

lethal (with Mcm6³)

lethal (with Mcm6⁴)

lethal (with Mcm6⁵)

lethal | recessive

partially lethal

Mcm6^K1214

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mcm6^GD4252

viable

Mcm6^K1214

viable, with Scer\GAL4^Mef2.PR

Mcm6^GD4252

viable, with Scer\GAL4^pnr-MD237

Mcm6^GD4252

viable, with Scer\GAL4^tin.CΔ4

Mcm6^GD4252

viable (with Mcm6²)

Mcm6^K1214

viable (with Mcm6³)

Mcm6^K1214

viable (with Mcm6⁴)

Mcm6^K1214

viable (with Mcm6⁵)

Mcm6^K1214

viable (with Mcm6^K1214)

Sterility

Allele

female sterile (with Mcm6²)

Mcm6^K1214

female sterile (with Mcm6³)

Mcm6^K1214

female sterile (with Mcm6⁴)

Mcm6^K1214

female sterile (with Mcm6⁵)

Mcm6^K1214

female sterile (with Mcm6^K1214)

female sterile | recessive

Mcm6^K1214

female sterile soma-dependent

Mcm6^K1214

Other Phenotypes

Allele

abnormal flight, with Scer\GAL4^Mef2.PR

Mcm6^GD4252

decreased body size | larval stage | recessive

Mcm6^unspecified

decreased cell number | oogenesis stage S10

Mcm6^K1214

decreased fecundity | female

Mcm6^K1214

visible, with Scer\GAL4^pnr-MD237

Mcm6^GD4252

wild-type

Mcm6^{Ubi-p63E.Tag:FLAG}

Phenotype manifest in

Allele

chaeta, with Scer\GAL4^pnr-MD237

Mcm6^GD4252

dorsal appendage

Mcm6^K1214

dorsal appendage | maternal effect

Mcm6^K1214

egg | maternal effect

Mcm6^K1214

egg chorion (with Mcm6²)

Mcm6^K1214

egg chorion (with Mcm6³)

Mcm6^K1214

egg chorion (with Mcm6⁴)

Mcm6^K1214

egg chorion (with Mcm6⁵)

Mcm6^K1214

egg chorion (with Mcm6^K1214)

egg chorion | maternal effect

Mcm6^K1214

follicle cell | oogenesis stage S10B

Mcm6^K1214

imaginal disc

Mcm6^unspecified

larval brain

nurse cell | oogenesis stage S10

Mcm6^K1214

X chromosome | oogenesis stage S10B

Mcm6^K1214

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (9)

Hsap\MCM6

14 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\MCM3

2 of 14

Hsap\MCMDC2

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (9)

Rnor\Mcm6

13 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Yes

Rnor\Mcmdc2

2 of 14

Yes

Mus musculus (laboratory mouse) (9)

Mmus\Mcm6

14 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

Yes

Mmus\Mcmdc2

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\mcm6.2

11 of 13

Yes

Xtro\mcm6

10 of 13

Yes

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Xtro\zmcm3

2 of 13

Xtro\hdlbp

1 of 13

Xtro\mcm3

1 of 13

Yes

Danio rerio (Zebrafish) (11)

Drer\mcm6

14 of 14

Yes

Drer\mcm6l

13 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Drer\mcm2

2 of 14

Drer\mcm7

2 of 14

Drer\mcmdc2

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\mcm-6

12 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\MCM6_ANOGA

12 of 12

Yes

Agam\AgaP_AGAP002580

4 of 12

Agam\AgaP_AGAP001135

3 of 12

Agam\AgaP_AGAP001901

3 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

Arabidopsis thaliana (thale-cress) (9)

Atha\MCM6

12 of 13

Yes

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

Yes

Atha\PRL

3 of 13

Atha\CHLI2

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\MCM6

12 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\mcm6

11 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

Escherichia coli (enterobacterium) (1)

Ecol\yifB

3 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mcm6. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

rec

7 of 13

dpa

6 of 13

6 of 13

6 of 13

6 of 13

6 of 13

2 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

MCM6; minichromosome maintenance complex component 6

model of lactose intolerance

IEA

(FlyBase, 2019-)

MCM9; minichromosome maintenance 9 homologous recombination repair factor

model of ovarian dysgenesis 4

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MCM6; minichromosome maintenance complex component 6

14 of 14

601806

LACTOSE INTOLERANCE, ADULT TYPE

lactose intolerance

MCM8; minichromosome maintenance 8 homologous recombination repair factor

4 of 14

608187

PREMATURE OVARIAN FAILURE 10; POF10

primary ovarian insufficiency 10

MCM2; minichromosome maintenance complex component 2

3 of 14

116945

DEAFNESS, AUTOSOMAL DOMINANT 70; DFNA70

autosomal dominant nonsyndromic deafness 70

MCM4; minichromosome maintenance complex component 4

3 of 14

602638

IMMUNODEFICIENCY 54; IMD54

immunodeficiency 54

MCM5; minichromosome maintenance complex component 5

3 of 14

602696

MEIER-GORLIN SYNDROME 8; MGORS8

Meier-Gorlin syndrome 8

MCM7; minichromosome maintenance complex component 7

3 of 14

600592

MCM9; minichromosome maintenance 9 homologous recombination repair factor

3 of 14

610098

OVARIAN DYSGENESIS 4; ODG4

ovarian dysgenesis 4

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Mcm6 - Cdc45

anti bait coimmunoprecipitation, mass spectrometry studies of complexes, anti tag coimmunoprecipitation, molecular weight estimation by silver staining, anti tag western blot, western blot, cosedimentation, electron microscopy

(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)

Mcm6 - Mcm2

cosedimentation, electron microscopy, experimental knowledge based

(Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011)

Mcm6 - Mcm3

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm6 - Mcm5

cosedimentation, electron microscopy, experimental knowledge based

(Costa et al., 2011, Guruharsha et al., 2011)

Mcm6 - Mcm7

experimental knowledge based, cosedimentation, electron microscopy

(Costa et al., 2011, Guruharsha et al., 2011)

Mcm6 - Psf1

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm6 - Psf2

anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy

(Costa et al., 2011, Moyer et al., 2006)

Mcm6 - Psf3

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm6 - Sld5

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm6 - chm

coimmunoprecipitation, anti tag western blot

(McConnell et al., 2012)

Mcm6 - dpa

experimental knowledge based, cosedimentation, electron microscopy

(Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011)

Mcm6 - lok

enzymatic study, autoradiography, molecular weight estimation by silver staining

(Ilves et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9V461 )

Linkouts

BioGRID - A database of protein and genetic interactions.

58089

DroID - A comprehensive database of gene and protein interactions.

FBgn0025815

MIST (protein-protein) - An integrated Molecular Interaction Database

31603

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

DNA replication

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-18

Cytogenetic map

6C4-6C5

Sequence location

X:6,674,387..6,677,176 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

6C4-6C5

Limits computationally determined from genome sequence between P{EP}Ctr1A^EP1364&P{EP}EP1445 and P{EP}C3G^EP1613

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

6C1-6C4

(Feger, 1999.2.1)

6C1-6C4

(determined by in situ hybridisation)

(Feger, 1999)

6B-6B

(determined by in situ hybridisation)

(Ohno et al., 1998)

5D5-6C12

(Komitopoulou et al., 1983)

Experimentally Determined Recombination Data

Location

1-18

(FlyBase, 2016)

1-18.6

(Comeron et al., 2012)

1-18.7

(Komitopoulou et al., 1983)

Left of (cM)

(Perrimon and Gans, 1983)

Right of (cM)

(Perrimon and Gans, 1983)

Notes

Stocks and Reagents

Stocks (14)

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (192)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0025815

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0025815

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells.

(Moyer et al., 2006)

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

Mutations in Mcm6 reduce chorion gene amplification.

(Komitopoulou et al., 1988)

Mutations of Mcm6 disrupt late oogenesis and affect normal amplification of all chorion genes.

(Perrimon et al., 1986)

Relationship to Other Genes

Source for database merge of

Source for merge of: Mcm6 fs(1)K1214

(Schwed et al., 2002)

Additional comments

The Mcm6 gene may have been derived from the rec gene by retroposition.

(Pan and Zhang, 2009)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG4039

(Çiçek et al., 2016, Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2010.12.1, Pan and Zhang, 2009)

DmMCM6

(Feger, 1999, Ohno, 1998.1.5, Ohno et al., 1998)

DmeMCM6

(Blanton et al., 2005)

MCM6

(Yheskel et al., 2023, Tain et al., 2021, Hua and Orr-Weaver, 2017, Bleichert et al., 2015, Crevel et al., 2011, Gallone et al., 2011, Ilves et al., 2010, Moshkin et al., 2009, Crevel et al., 2007, Hong et al., 2007, Claycomb et al., 2002, Crevel et al., 2001)

McM6

(De Lucia et al., 2005)

dMcm6

(Sahashi et al., 2013)

fs(1)K1214

(Calvi et al., 1998, Orr-Weaver, 1991, Grossniklaus et al., 1989, Komitopoulou et al., 1988, Perrimon et al., 1986, Perrimon and Gans, 1983)

mcm6

(Raicu et al., 2024, Gause et al., 2010, Claycomb and Orr-Weaver, 2005, Claycomb et al., 2004, Lee and Orr-Weaver, 2003)

Name Synonyms

Minichromosome maintenance 6

(Graves et al., 2020, Rollmann et al., 2008, Ohno et al., 1998)

female sterile(1)K1214

Secondary FlyBase IDs

FBgn0000821

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

modENCODE_replication_factor_ChIP

genome binding

Genome-wide localization of essential replication factors characterized by ChIP-Seq and ChIP-chip.

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31603

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V461

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V461

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V461

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31603/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025815

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025815

FlyMine - An integrated database for Drosophila genomics

FBgn0025815

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4039

MARRVEL_MODEL - MARRVEL (model organism gene)

31603

PDB - An information portal to biological macromolecular structures

Linkouts

BioGRID - A database of protein and genetic interactions.

58089

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0025815

DroID - A comprehensive database of gene and protein interactions.

FBgn0025815

DRSC - Results frm RNAi screens

FBgn0025815

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025815

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0025815

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4039

Flygut - An atlas of the Drosophila adult midgut

FBgn0025815

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0025815

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC011200

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

DNA replication

MIST (protein-protein) - An integrated Molecular Interaction Database

31603

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (114)

The following external sites may use different assemblies or annotations than FlyBase.

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