FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Exo84
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General Information
Symbol
Dmel\Exo84
Species
D. melanogaster
Name
Exocyst 84
Annotation Symbol
CG6095
Feature Type
protein_coding_gene
FlyBase ID
FBgn0290418
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
Exocyst 84 (Exo84) encodes an exocyst component involved in epithelial polarity. It mediates the apical localization of the transmembrane protein encoded by crb. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)
All Summaries
Also Known As

onr, onion rings, l(3)CHa

Key Links
Genomic Location
Cytogenetic map
96F10-96F10
Sequence location
Recombination map
3-89
RefSeq locus
NT_033777 REGION:26049731..26052257
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (17 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (16 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in actomyosin contractile ring contraction
inferred from mutant phenotype
(Giansanti et al., 2004)
involved_in endocytic recycling
inferred from mutant phenotype
(Blankenship et al., 2007)
involved_in establishment or maintenance of cell polarity
inferred from mutant phenotype
(Campbell et al., 2009)
involved_in maintenance of epithelial cell apical/basal polarity
inferred from mutant phenotype
(Blankenship et al., 2007)
involved_in meiosis I cytokinesis
inferred from mutant phenotype
(Giansanti et al., 2004)
involved_in meiosis II cytokinesis
inferred from mutant phenotype
(Giansanti et al., 2004)
involved_in meiotic spindle organization
inferred from mutant phenotype
(Giansanti et al., 2004)
involved_in protein localization to plasma membrane
inferred from mutant phenotype
(Blankenship et al., 2007)
involved_in spermatid development
inferred from mutant phenotype
(Fabian et al., 2010)
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
involved_in exocytosis
inferred from electronic annotation with InterPro:IPR033961
(InterPro Project Members, 2004-)
involved_in Golgi to plasma membrane transport
inferred from biological aspect of ancestor with PANTHER:PTN000484343
(GO Reference Genome Project, 2011-)
involved_in intracellular protein localization
inferred from biological aspect of ancestor with PANTHER:PTN000484343
(GO Reference Genome Project, 2011-)
involved_in neurotransmitter secretion
non-traceable author statement
(Lloyd et al., 2000)
involved_in synaptic vesicle docking
non-traceable author statement
(Lloyd et al., 2000)
involved_in synaptic vesicle targeting
non-traceable author statement
(Lloyd et al., 2000)
involved_in vesicle-mediated transport
inferred from sequence or structural similarity
(Littleton, 2000)
Cellular Component (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
part_of exocyst
inferred from direct assay
(Sommer et al., 2005)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of exocyst
inferred from sequence or structural similarity
(Littleton, 2000)
non-traceable author statement
(Lloyd et al., 2000)
inferred from biological aspect of ancestor with PANTHER:PTN000484343
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR033961
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Exocyst 84 (Exo84) encodes an exocyst component involved in epithelial polarity. It mediates the apical localization of the transmembrane protein encoded by crb. [Date last reviewed: 2019-07-11]
Gene Group (FlyBase)
EXOCYST -
The exocyst complex is composed of eight proteins. It is involved tethering secretory vesicles to the plasma membrane and may regulate other aspects of intracellular trafficking such as SNARE assembly, cytokinesis and endocytic recycling. The exocyst complex is a member of the Complex Associated with Tethering Containing Helical Rods (CATCHR) family. (Adapted from FBrf0219543).
Summary (Interactive Fly)

exocyst complex 84-kDa subunit Pleckstrin Homology (PH) domain protein - involved in apical polarity of epithelial tissue - the exocyst complex functions in polarized exocytosis

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Exo84 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7KRZ3)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.48

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Exo84-RA
FBtr0084977
NM_143197
2243
671
Exo84-RB
FBtr0084978
NM_206576
2246
672
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Exo84-PA
FBpp0084350
76.5
671
7.46
Q9VBI4
NP_651454
AAF56558
Exo84-PB
FBpp0084351
76.7
672
7.64
Q7KRZ3
NP_996299
AAS65219
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Exo84 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.01

Transcript Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of exocyst
inferred from direct assay
(Sommer et al., 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Exo84 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Exo84
Transgenic constructs containing regulatory region of Exo84
Aberrations (Deficiencies and Duplications) ( 6 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(3R)BSC751)
lethal (with Df(3R)ED6232)
lethal (with Df(3R)Exel6204)
lethal | recessive
lethal - all die during embryonic stage (with Df(3R)Espl3)
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal cell polarity | embryonic stage 16
abnormal cytokinesis | male (with Df(3R)Espl1)
abnormal cytokinesis | male | recessive
abnormal developmental rate | larval stage, with Scer\GAL4phtm.PO
abnormal meiotic cell cycle | male (with Df(3R)Espl1)
abnormal meiotic cell cycle | male | recessive
abnormal mitotic cell cycle | male (with Df(3R)Espl1)
abnormal mitotic cell cycle | male | recessive
abnormal neuroanatomy | larval stage, with Scer\GAL4elav.PU
some die during embryonic stage (with Df(3R)Espl3)
some die during larval stage, with Scer\GAL4phtm.PO
some die during pharate adult stage, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
contractile ring & meiotic telophase I | male
contractile ring & meiotic telophase I | male (with Df(3R)Espl1)
contractile ring & meiotic telophase II | male
contractile ring & meiotic telophase II | male (with Df(3R)Espl1)
embryo | embryonic stage 16
embryonic/first instar larval cuticle | heat sensitive (with Df(3R)Espl3)
embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4elav.PU
epidermal cell
mesonotum, with Scer\GAL4pnr-MD237
Nebenkern
Nebenkern (with Df(3R)Espl1)
NMJ bouton | increased number | larval stage, with Scer\GAL4elav.PU
presynaptic active zone | larval stage, with Scer\GAL4elav.PU
spermatid (with Df(3R)Espl3)
spermatid & nucleus | supernumerary
spermatid & nucleus | supernumerary (with Df(3R)Espl1)
spermatid cyst (with Df(3R)Espl3)
spindle & meiotic telophase I | male
spindle & meiotic telophase I | male (with Df(3R)Espl1)
spindle & meiotic telophase II | male
spindle & meiotic telophase II | male (with Df(3R)Espl1)
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (13)
Hsap\EXOC8
14 of 14
Yes
Yes
Hsap\FARP1
1 of 14
No
No
Hsap\FARP2
1 of 14
No
No
Hsap\FGD1
1 of 14
No
No
Hsap\FGD2
1 of 14
No
No
Hsap\FGD3
1 of 14
No
No
Hsap\FGD4
1 of 14
No
No
Hsap\FGD5
1 of 14
No
No
Hsap\FGD6
1 of 14
No
No
Hsap\MTMR4
1 of 14
No
No
2  
Hsap\PLEKHF1
1 of 14
No
No
Hsap\PLEKHF2
1 of 14
No
No
Hsap\ZFYVE9
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Exoc8
14 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Exoc8
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (9)
Xtro\exoc8
7 of 13
Yes
Yes
Xtro\arhgef39
1 of 13
No
No
Xtro\farp1
1 of 13
No
No
Xtro\fgd1
1 of 13
No
No
Xtro\mtmr4
1 of 13
No
No
Xtro\plekhf1
1 of 13
No
No
Xtro\plekhf2
1 of 13
No
No
Xtro\zfyve16
1 of 13
No
No
Xtro\zfyve26
1 of 13
No
No
Danio rerio (Zebrafish) (1)
Drer\exoc8
14 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\exoc-8
13 of 14
Yes
Yes
Cele\lst-2
1 of 14
No
No
Cele\tag-77
1 of 14
No
No
Cele\ZK632.12
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP004207
12 of 12
Yes
Yes
Agam\AgaP_AGAP001287
1 of 12
No
No
Agam\AgaP_AGAP008932
1 of 12
No
No
Agam\AgaP_AGAP010307
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (5)
Atha\AT1G10180
2 of 13
Yes
Yes
Atha\AT1G10385
2 of 13
Yes
Yes
Atha\EXO84B
2 of 13
Yes
Yes
Atha\AT5G12350
1 of 13
No
No
Atha\AT5G19420
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\EXO84
7 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPAC6F12.08c
4 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Exo84. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (2)
fab1
1 of 13
rush
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    EXOC8; exocyst complex component 8
    14 of 14
    615283
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Exo84
      enhanceable
      crb
      (Blankenship et al., 2007)
      Exo84
      suppressible
      crb
      (Blankenship et al., 2007)
      Exo84
      suppressible
      dlg1
      (Blankenship et al., 2007)
      Exo84
      enhanceable
      l(2)gl
      (Blankenship et al., 2007)
      Exo84
      suppressible
      l(2)gl
      (Blankenship et al., 2007)
      Exo84
      enhanceable
      Sec5
      (Blankenship et al., 2007)
      Exo84
      enhanceable
      Sec6
      (Blankenship et al., 2007)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Linkouts
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map
      3-89
      Cytogenetic map
      96F10-96F10
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      96F10-96F10
      Limits computationally determined from genome sequence between P{PZ}l(3)rQ197rQ197 and P{lacW}scribj7B3
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      Location based on deficiency mapping.
      (Giansanti et al., 2004)
      96F7-97A4
      (Giansanti et al., 2004)
      Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.
      (Lloyd et al., 2000)
      FlyBase curator comment: FBrf0127207 reports cytological location as 69F, however FBrf0187825 subsequently reported FBgn0042644 to correspond to "CG6095" at 96F, suggesting that the '69F' in FBrf0127207 was a typographical error.
      (Lloyd et al., 2000)
      Experimentally Determined Recombination Data
      Location

      3-89

      (FlyBase, 2016)

      3-87.4

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (7)
      Genomic Clones (22)
      cDNA Clones (25)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            onr is required for ingression of the cleavage furrow during spermatogenesis. onr is involved in membrane trafficking.

            (Blankenship et al., 2004)

            Mutants are male sterile.

            (Blankenship et al., 2004)

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)

            Spermatids contain multiple normal-sized nuclei and large mitochondrial derivatives, reflecting a failure in cytokinesis. Mutants also have a striking defect in spermatid elongation. In onr mutant cells, the contractile ring and central spindle assemble but do not constrict fully.

            (Farkas et al., 2001)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: Exo84 l(3)CHa

            (Popodi et al., 2025.8.28)

            Source for merge of: exo84 onr

            (Blankenship et al., 2007)

            Source for merge of: exo84 CG6095

            (Sommer et al., 2005)

            Source for merge of: CG6095 anon-WO0153538.55

            (FlyBase, 1996-)

            Source for merge of: Exo84 exo84

            (FlyBase, 1992-)
            Additional comments

            Source for merge of CG6095 anon-WO0153538.55 was sequence comparison ( date:051113 ).

            (FlyBase, 1996-)
            Nomenclature History
            Source for database identify of

            Source for identity of: Exo84 exo84

            (FlyBase, 2009-)
            Nomenclature comments
            Etymology

            Named "Exo84" after the S. cerevisiae ortholog.

            (FlyBase, 2009-)
            Synonyms and Secondary IDs (14)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 30 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            References (83)