Reference Report

Reference
Citation	Zaffran, S., Reim, I., Qian, L., Lo, P.C., Bodmer, R., Frasch, M. (2006). Cardioblast-intrinsic Tinman activity controls proper diversification and differentiation of myocardial cells in Drosophila. Development 133(20): 4073--4083. (Export to RIS)
FlyBase ID	FBrf0195151
Publication Type	Research paper
PubMed ID	16987868
PubMed Abstract	The NK homeobox gene tinman (tin) is required for the specification of the cardiac, visceral muscle and somatic muscle progenitors in the early dorsal mesoderm of Drosophila. Like its vertebrate counterpart Nkx2.5, the expression of tin is maintained in cardiac cells during cardiac maturation and differentiation; however, owing to the complete lack of a dorsal vessel in tin mutant embryos, the function of tin in these cells has not been defined. Here we show that myocardial cells and dorsal vessels can form even though they lack Tin, and that viable adults can develop, as long as Tin is provided in the embryonic precardiac mesoderm. However, embryos in which tin expression is specifically missing from cardial cells show severe disruptions in the normal diversification of the myocardial cells, and adults exhibit severe defects in cardiac remodeling and function. Our study reveals that the normal expression and activity of Tin in four of the six bilateral cardioblasts within each hemisegment of the heart allows these cells to adopt a cell fate as ;working' myocardium, as opposed to a fate as inflow tract (ostial) cells. This function of tin involves the repression of Dorsocross (Doc) T-box genes and, hence, the restriction of Doc to the Tin-negative cells that will form ostia. We conclude that tin has a crucial role within myocardial cells that is required for the proper diversification, differentiation, and post-embryonic maturation of cardiomyocytes, and we present a pathway involving regulatory interactions among seven-up, midline, tinman and Dorsocross that establishes these developmental events upon myocardial cell specification.
DOI	10.1242/dev.02586
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Secondary IDs
Language of Publication	English
Additional Languages of Abstract
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Parent Publication
Publication Type	Journal
Abbreviation	Development
Title	Development
Publication Year	1987-
ISBN/ISSN	0950-1991
Data from Reference
Aberrations (3)
	Df(3L)29A6 Df(3L)DocA Df(3R)GC14
Alleles (15)
	Doc1^Scer\UAS.cRa Doc2^Scer\UAS.cRa Ecol\lacZ^svp-AE127 mid¹ Mmus\Nkx2-5^{Scer\UAS.T:Zzzz\FLAG} Scer\GAL4^{HtΔD.slou.Mef2} svp^AE127 svp^I.Scer\UAS tin³⁴⁶ tin^AB tin^ABD tin^D tin^EC40 tin^Scer\UAS.cYa w^+mC
Constructs (9)
	P{S59-Mef2-HtΔD-Gal4} P{tin-AB} P{tin-ABD} P{tin-D} P{UAS-Doc1.R} P{UAS-Doc2.R} P{UAS-Nkx2.5.FLAG} P{UAS-svp.I} P{UAS-tin.Y}
Genes (27)
	bin Doc1 Doc2 Doc3 Ecol\lacZ eve Gαo H15 Hand hth lbe Mef2 mid Mmus\Nkx2-5 odd Scer\GAL4 Sur svp T:Zzzz\FLAG tin Tl Tm2 w wg zfh1 α-Spec βTub60D
Insertions (3)
	P{lwB}svp^AE127 P{tin-ABD}T003-1B2 P{tin-ABD}T003-1C1
Natural transposons (1)
	P-element