FlyBase Gene Report: Dmel\Gβ76C

General Information

Symbol

Dmel\Gβ76C

Species

D. melanogaster

Name

G protein beta subunit 76C

Annotation Symbol

CG8770

Feature Type

protein_coding_gene

FlyBase ID

FBgn0004623

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Summary

G protein β-subunit 76C (Gβ76C) encodes a protein that forms a Gβ-γ complex that is anchored to the plasma membrane via lipid modification of the γ-subunit . The Gβ-γ complex plays an essential role in phototransduction by presenting the Gqα-subunit to rhodopsin GPCRs for efficient activation, and preventing spontaneous GDP-GTP exchange on the Gqα-subunit. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

Gβe, Gbe, Gβ_e, Gβ, G protein β-subunit 76C

Key Links

Genomic Location

Cytogenetic map

76C1-76C1

Sequence location

3L:19,687,637..19,689,992 [+]

Recombination map

3-46

RefSeq locus

NT_037436 REGION:19687637..19689992

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables phospholipase activator activity

inferred from mutant phenotype

(Running Deer et al., 1995)

enables protein heterodimerization activity

inferred from physical interaction with FLYBASE:Gγ30A; FB:FBgn0267252

(Schulz et al., 1999)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables signaling receptor complex adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000457487

(GO Reference Genome Project, 2011-)

Biological Process (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in G protein-coupled receptor signaling pathway

inferred from direct assay

(Yarfitz et al., 1994)

involved_in negative regulation of smoothened signaling pathway

inferred from genetic interaction with FLYBASE:Gβ13F; FB:FBgn0001105,FLYBASE:Smurf; FB:FBgn0029006

(Li et al., 2018)

involved_in phospholipase C-activating opsin-mediated signaling pathway

inferred from mutant phenotype

(Dolph et al., 1994)

inferred from direct assay

(Elia et al., 2005)

involved_in phototransduction

inferred from mutant phenotype

(Yarfitz et al., 1994)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in G protein-coupled receptor signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN000457487

(GO Reference Genome Project, 2011-)

involved_in negative regulation of opsin-mediated signaling pathway

traceable author statement

(Montell, 1999, Zuker, 1996)

involved_in signal transduction

inferred from electronic annotation with InterPro:IPR016346

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Schulz et al., 1999)

part_of heterotrimeric G-protein complex

inferred from direct assay

(Yarfitz et al., 1994)

located_in rhabdomere

inferred from direct assay

(Schulz et al., 1999, Yarfitz et al., 1994)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000457487

(GO Reference Genome Project, 2011-)

part_of heterotrimeric G-protein complex

inferred from biological aspect of ancestor with PANTHER:PTN000457487

(GO Reference Genome Project, 2011-)

located_in rhabdomere

traceable author statement

(Montell, 1999)

Gene Group (FlyBase)

HETEROTRIMERIC G-PROTEIN BETA SUBUNITS

Pathway (FlyBase)

NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY

Protein Family (UniProt)

Belongs to the WD repeat G protein beta family. (P29829)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

HETEROTRIMERIC G-PROTEIN BETA SUBUNITS -: The Heterotrimeric G-protein beta subunits (Gβ) contain seven WD-40 repeats. Gβ associates with Gγ (Gβγ) to form a functional heterodimer. When Gα is bound to GDP, Gβγ associates with Gα to form an heterotrimer (Gαβγ), which prevents Gβγ from activating its effectors. (Adapted from FBrf0209904 and FBrf0183798).

Pathway (FlyBase)

NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY -: Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.

Protein Function (UniProtKB)

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

(UniProt, P29829)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Gβ76C for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P29829)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Gβ76C-RA

FBtr0074949

NM_078996

1466

346

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Gβ76C-PA

FBpp0074717

38.3

346

6.77

P29829

NP_523720

AAF49124

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

346 (aa); 39 (kD observed)

(Yarfitz et al., 1991)

Comments

Gβ76C protein, Gα49B protein, and Gγ30A protein are all expressed in the same pattern supporting the hypothesis that Gγ30A is part of a heterotrimeric eye-specific G protein.

(Schulz et al., 1999)

External Data

Subunit Structure (UniProtKB)

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with Ggamma30A/Guanine nucleotide-binding protein subunit gamma-e (By similarity).

(UniProt, P29829)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gβ76C using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gbeta76C_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.62

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

Bolwig organ

(Fisher et al., 2012)

adult stage

Johnston organ

(Senthilan et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Yarfitz et al., 1991)

adult head

(Yarfitz et al., 1991)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

(Boto et al., 2010)

Additional Descriptive Data

RT-PCR analysis shows that Gβ13F transcript is only detected in the adult head.

(Boto et al., 2010)

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

medulla

(Schulz et al., 1999)

lamina

(Schulz et al., 1999)

photoreceptor neuron

(Schulz et al., 1999)

eye

(Yarfitz et al., 1991)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Gβ76C protein is localized to the photoreceptor cells of the retina. It is also detected in the lamina and the medulla, presumably in axons of the photoreceptor cells that project to these optic ganglia.

(Schulz et al., 1999)

A monoclonal antibody generated against Gβ13F protein also recognizes Gβ76C protein. The antibody stains the brain neuropil and cortex, the eye, and the thoracic ganglion; the eye-specific staining is due to Gβ76C protein.

(Yarfitz et al., 1991)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Schulz et al., 1999)

part_of heterotrimeric G-protein complex

inferred from direct assay

(Yarfitz et al., 1994)

located_in rhabdomere

inferred from direct assay

(Schulz et al., 1999, Yarfitz et al., 1994)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Gβ76C in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Gβ76C

View exonic expression by tissue for Dmel\Gβ76C

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004623

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40148/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004623

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004623

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004623

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8770

Flygut - An atlas of the Drosophila adult midgut

FBgn0004623

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Gβ76C

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Gβ76C

Transgenic constructs containing regulatory region of Gβ76C

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3L)Exel6135

(Ryder, 2004.4.26)

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Gβ76C^GD13785

viable, with Scer\GAL4^Mef2.PR

Gβ76C^GD13785

viable, with Scer\GAL4^pnr-MD237

Gβ76C^GD13785

Other Phenotypes

Allele

abnormal neurophysiology

wild-type

Gβ76C^+tDa

Phenotype manifest in

Allele

photoreceptor neuron

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (21)

Hsap\GNB1

6 of 14

Yes

Hsap\GNB2

6 of 14

Yes

Hsap\GNB3

6 of 14

Yes

Hsap\GNB4

6 of 14

Yes

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\WDR46

1 of 14

Hsap\WDR77

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (13)

Rnor\Gnb1

6 of 14

Yes

Rnor\Gnb2

6 of 14

Yes

Rnor\Gnb3

6 of 14

Yes

5 of 14

5 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Wdr77

1 of 14

Mus musculus (laboratory mouse) (12)

Mmus\Gnb1

6 of 14

Yes

Mmus\Gnb2

6 of 14

Yes

Mmus\Gnb3

6 of 14

Yes

5 of 14

4 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Wdr77

1 of 14

Xenopus tropicalis (Western clawed frog) (12)

Xtro\gnb1

5 of 13

Yes

Xtro\gnb3

4 of 13

Yes

4 of 13

4 of 13

4 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (17)

Drer\gnb2

6 of 14

Yes

Drer\gnb3a

6 of 14

Yes

Drer\gnb3b

6 of 14

Yes

Drer\gnb4a

6 of 14

Yes

5 of 14

5 of 14

5 of 14

5 of 14

4 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\wdr32

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\gpb-1

5 of 14

Yes

Cele\gpb-2

5 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\wdr-46

1 of 14

Anopheles gambiae (African malaria mosquito) (15)

Agam\AgaP_AGAP001506

12 of 12

Yes

5 of 12

5 of 12

5 of 12

5 of 12

5 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (14)

Atha\AGB1

7 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\MAC3A

1 of 13

Atha\TAF5

1 of 13

Atha\WDR55

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (6)

Scer\STE4

8 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\git5

7 of 12

Yes

Spom\skb15

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Gβ76C. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (15)

9 of 13

9 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

vls

1 of 13

wds

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 6 )

Human Ortholog

Disease

Evidence

References

GNB3; G protein subunit beta 3

model of essential hypertension

IEA

(FlyBase, 2019-)

model of congenital stationary night blindness 1H

IEA

(FlyBase, 2019-)

GNB4; G protein subunit beta 4

model of Charcot-Marie-Tooth disease dominant intermediate F

IEA

(FlyBase, 2019-)

GNB1; G protein subunit beta 1

model of acute lymphoblastic leukemia

IEA

(FlyBase, 2019-)

model of myelodysplastic syndrome

IEA

(FlyBase, 2019-)

model of autosomal dominant intellectual developmental disorder 42

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

GNB1; G protein subunit beta 1

6 of 14

139380

GNB2; G protein subunit beta 2

6 of 14

139390

GNB3; G protein subunit beta 3

6 of 14

139130

GNB4; G protein subunit beta 4

6 of 14

610863

CHARCOT-MARIE-TOOTH DISEASE, DOMINANT INTERMEDIATE F; CMTDIF

Charcot-Marie-Tooth disease dominant intermediate F

GNB5; G protein subunit beta 5

5 of 14

604447

Lodder-Merla syndrome type 1 with impaired intellectual development and cardiac arrhythmia

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Gβ76C - Gprk1

anti tag coimmunoprecipitation, western blot, pull down, autoradiography

(Lee et al., 2004)

Gβ76C - Gprk2

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Gβ76C

suppressible

Gαq

(Elia et al., 2005)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Gαq

suppressible

Gβ76C

(Elia et al., 2005)

External Data

Subunit Structure (UniProtKB)

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with Ggamma30A/Guanine nucleotide-binding protein subunit gamma-e (By similarity).

(UniProt, P29829 )

Linkouts

BioGRID - A database of protein and genetic interactions.

65418

DroID - A comprehensive database of gene and protein interactions.

FBgn0004623

MIST (protein-protein) - An integrated Molecular Interaction Database

40148

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF HEDGEHOG SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Adrenaline,noradrenaline inhibits insulin secretion

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Phototransduction - fly

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P29829

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-46

Cytogenetic map

76C1-76C1

Sequence location

3L:19,687,637..19,689,992 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

76C1-76C1

Limits computationally determined from genome sequence between P{lacW}l(3)L3809^L3809 and P{lacW}l(3)L1243^L1243&P{lacW}Mi-2^j3D4

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

76C1-76C1

(determined by in situ hybridisation)

(Yarfitz et al., 1991)

Experimentally Determined Recombination Data

Location

3-46

(FlyBase, 2016)

3-43.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (9)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

1468

w¹¹¹⁸; Gβ76C¹

28507

y¹ v¹; P{TRiP.JF03127}attP2

42256

w^*; Gβ76C²/TM6B, Tb¹

86455

y¹ w^*; TI{CRIMIC.TG4.1}Gβ76C^{CR01577-TG4.1}

FlyORF

F003129

M{UAS-Gβ76C.ORF.3xHA.GW}ZH-86Fb

Kyoto

106327

w¹¹¹⁸; Gβ76C¹

8770R-3

v104745

v28870

w¹¹¹⁸; P{GD13785}v28870

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (46)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

HL07966

Other clones

DPiM_HL07966

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0004623

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0004623

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Excess of Gβ76C over Gα49B prevents dark, spontaneous activity of photoreceptors.

(Elia et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

dsRNA made from templates generated with primers against this gene tested in a cl-8 cell reporter assay to examine any role of Gβ76C in the hh signalling pathway.

(Lum, 2003)

Light-sensitive norpA phospholipase C activity in Drosophila head membranes is dependent on normal Gβ76C function.

(Running Deer et al., 1995)

Gβ76C is an eye specific G-protein β subunit which is essential for G-protein-receptor coupling in vivo. Mutants are also severely defective in the deactivation of the light response demonstrating an essential role for the Gβ76C subunit in terminating the active state of the signalling cascade.

(Dolph et al., 1994)

An in situ ³⁵S-labelled guanosine 5'-3-0-(thio)triphosphate (³⁵S GTPγS) binding procedure can assay light stimulated G protein activity in eyes. Results demonstrate Gβ76C functions as the β subunit of a heterotrimeric G protein that couples photoactivation of rhodopsin to downstream components in the phototransduction cascade.

(Yarfitz et al., 1994)

Gβ76C has been cloned and sequenced, and its expression pattern has been analysed.

(Yarfitz et al., 1991)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (26)

Reported As

Symbol Synonym

CG8770

(Hanlon and Andrew, 2015, Boto et al., 2010, Khanna et al., 2010, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Perkins et al., 2009)

DGqβ

(Kosloff et al., 2003)

G_β76C

(Boto et al., 2010)

G_β

(Montell, 2000)

G_βe

(Kosloff et al., 2003)

G^q

(Delmas et al., 2004)

Galpha76C

(Yang et al., 2005)

Gbe

(Sun et al., 2008, Sapir et al., 2005, Zemelman et al., 2002, Yarfitz et al., 1994, Yarfitz et al., 1993, Yarfitz et al., 1991)

Gbeta76C

(Gene Disruption Project members, 2018-, Chatterjee et al., 2014, Japanese National Institute of Genetics, 2012.5.21, Cooper et al., 2010, Yang et al., 2005, Pedra et al., 2004, Giot et al., 2003, Lum, 2003)

Gbeta76c

(Riveron et al., 2013)

Gqβ

(Montell, 1999)

Gqβe

(Katz and Minke, 2018)

Gβ

(Krishnan et al., 2015, Yamashita et al., 2005, Lee et al., 2004, Zemelman et al., 2002)

Gβ76C

(Hsu et al., 2024, Bossen et al., 2023, Hodge et al., 2022, Liu et al., 2022, Sebastian et al., 2022, Liu et al., 2021, Li et al., 2018, Hanlon and Andrew, 2015, Im et al., 2015, Rister et al., 2015, Vecsey et al., 2014, Senthilan et al., 2012, Boto et al., 2010, Yao and Carlson, 2010, Yi et al., 2008, Landry et al., 2007, Stuart et al., 2007)

Gβ76c

(Zhou et al., 2014)

Gβ_e

(Wang et al., 2008, Frechter et al., 2007, Elia et al., 2005, Harris and Lim, 2001, Schulz et al., 1999)

Gβ_76C

(Li et al., 2021)

Gβe

(Hanlon and Andrew, 2015, Krishnan et al., 2015, Hu et al., 2012, Boto et al., 2010, Schillo et al., 2004, Xu et al., 2004, Hardie et al., 2002, Huber et al., 1999, Montell, 1999, Running Deer et al., 1995, Dolph et al., 1994, Yarfitz et al., 1994)

Gβ{e}

(Elia et al., 2005)

eGβ

(Zelhof et al., 2003)

eye Gβ

(Zelhof et al., 2003)

gbe

(Hardie, 2001, Alvarez et al., 1996, Zuker, 1996, Zuker, 1992, Ranganathan et al., 1991)

Name Synonyms

G protein beta subunit 76C

G protein β-subunit 76C

(Khanna et al., 2010)

Gβ eye

(Yarfitz et al., 1991)

guanine nucleotide regulatory protein beta subunit

(Yarfitz, 1994.5.27)

Secondary FlyBase IDs

FBgn0016651

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40148

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P29829

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P29829

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P29829

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0004623

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40148/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0004623

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0004623

FlyMine - An integrated database for Drosophila genomics

FBgn0004623

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8770

MARRVEL_MODEL - MARRVEL (model organism gene)

40148

Linkouts

BioGRID - A database of protein and genetic interactions.

65418

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0004623

DroID - A comprehensive database of gene and protein interactions.

FBgn0004623

DRSC - Results frm RNAi screens

FBgn0004623

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Gbeta76C_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0004623

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0004623

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8770

Flygut - An atlas of the Drosophila adult midgut

FBgn0004623

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0004623

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014410

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Phototransduction - fly

MIST (protein-protein) - An integrated Molecular Interaction Database

40148

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

Adrenaline,noradrenaline inhibits insulin secretion

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P29829

References (101)

The following external sites may use different assemblies or annotations than FlyBase.

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