FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\Nup154
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General Information
Symbol
Dmel\Nup154
Species
D. melanogaster
Name
Nucleoporin 154kD
Annotation Symbol
CG4579
Feature Type
protein_coding_gene
FlyBase ID
FBgn0021761
Gene Model Status
Current
Stock Availability
10 publicly available
Gene Summary
Component of the nuclear pore complex (PubMed:17410542). Has a role in the organization of the inner nuclear membrane proteins at the nuclear envelope (PubMed:22718353). In germ cells, plays a role in the nuclear localization of components of the dpp signaling pathways, such as Medea and phosphorylated Mad (PubMed:21696798). Binds to chromatin, and together with Nup62 and Nup93-1, contributes to karyosome morphology and chromatin organization including attachment to the nuclear envelope in oocytes and nurse cells (PubMed:22718353, PubMed:26341556). Has a role in female fertility including egg chamber development; in nurse cells, has a role in the organization of F-actin in subcortical and cytoplasmic actin filaments important for the transfer of cytoplasm from nurse cells to the growing oocytes (PubMed:10511559, PubMed:17277377, PubMed:17410542, PubMed:9732281). Has a role in male spermatogenesis and fertility (PubMed:10511559, PubMed:9732281). Has a role in germ line cell proliferation (PubMed:21696798). (UniProt, Q9V463)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

zk, tlp, tulipano, nup32D, zonder kloten

Key Links
Genomic Location
Cytogenetic map
32C1-32C1
Sequence location
Recombination map
2-44
RefSeq locus
NT_033779 REGION:11004820..11010921
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (32 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables chromatin binding
inferred from direct assay
(Busayavalasa et al., 2012)
enables protein binding
inferred from physical interaction with FLYBASE:Nup93-1; FB:FBgn0027537
(Busayavalasa et al., 2012)
inferred from physical interaction with UniProtKB:Q9VMA3
(Grimaldi et al., 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables structural constituent of nuclear pore
inferred from electronic annotation with InterPro:IPR004870
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000039103
(GO Reference Genome Project, 2011-)
Biological Process (23 terms)
Terms Based on Experimental Evidence (19 terms)
CV Term
Evidence
References
involved_in actin filament organization
inferred from mutant phenotype
(Riparbelli et al., 2007)
involved_in eye development
inferred from mutant phenotype
(Kiger et al., 1999)
involved_in germ cell development
inferred from mutant phenotype
(Kiger et al., 1999)
involved_in growth of a germarium-derived egg chamber
inferred from mutant phenotype
(Gigliotti et al., 1998)
involved_in male meiotic nuclear division
inferred from mutant phenotype
(Gigliotti et al., 1998)
involved_in oocyte growth in germarium-derived egg chamber
inferred from genetic interaction with UniProtKB:Q9VMA3
(Grimaldi et al., 2007)
involved_in oocyte growth
inferred from mutant phenotype
(Riparbelli et al., 2007, Gigliotti et al., 1998)
involved_in oocyte karyosome formation
inferred from mutant phenotype
(Breuer and Ohkura, 2015)
involved_in oogenesis
inferred from mutant phenotype
(Gigliotti et al., 1998)
involved_in ovarian nurse cell to oocyte transport
inferred from mutant phenotype
(Riparbelli et al., 2007)
involved_in positive regulation of germ cell proliferation
inferred from mutant phenotype
(Colozza et al., 2011)
involved_in positive regulation of protein import into nucleus
inferred from mutant phenotype
(Colozza et al., 2011)
involved_in positive regulation of protein localization to nucleus
inferred from direct assay
(Gigliotti et al., 1998)
involved_in protein localization to nuclear inner membrane
inferred from mutant phenotype
(Busayavalasa et al., 2012)
involved_in regulation of nurse cell apoptotic process
inferred from mutant phenotype
(Riparbelli et al., 2007)
involved_in regulation of oogenesis
inferred from mutant phenotype
(Grimaldi et al., 2007, Riparbelli et al., 2007)
involved_in regulation of protein localization to membrane
inferred from mutant phenotype
(Breuer and Ohkura, 2015)
involved_in spermatogenesis
inferred from mutant phenotype
(Gigliotti et al., 1998)
involved_in wing disc development
inferred from mutant phenotype
(Kiger et al., 1999)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in nucleocytoplasmic transport
inferred from electronic annotation with InterPro:IPR004870
(InterPro Project Members, 2004-)
involved_in protein import into nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000039103
(GO Reference Genome Project, 2011-)
involved_in protein localization to nuclear inner membrane
inferred from biological aspect of ancestor with PANTHER:PTN000039103
(GO Reference Genome Project, 2011-)
involved_in RNA export from nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000039103
(GO Reference Genome Project, 2011-)
involved_in transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery
inferred from biological aspect of ancestor with PANTHER:PTN000039103
(GO Reference Genome Project, 2011-)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Busayavalasa et al., 2012)
inferred from direct assay
(Riparbelli et al., 2007)
located_in nuclear envelope
inferred from direct assay
(Busayavalasa et al., 2012, Grimaldi et al., 2007)
inferred from direct assay
(Capelson et al., 2010)
located_in nuclear periphery
inferred from direct assay
(Riparbelli et al., 2007, Gigliotti et al., 1998)
part_of nuclear pore
inferred from direct assay
(Riparbelli et al., 2007)
located_in nucleus
inferred from direct assay
(Riparbelli et al., 2007, Gigliotti et al., 1998)
inferred from direct assay
(Busayavalasa et al., 2012)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of nuclear pore inner ring
inferred from biological aspect of ancestor with PANTHER:PTN000039103
(GO Reference Genome Project, 2011-)
part_of nuclear pore
inferred from electronic annotation with InterPro:IPR004870
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the non-repetitive/WGA-negative nucleoporin family. (Q9V463)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
NUCLEAR PORE COMPLEX -
The Nuclear Pore Complex (NPC) is a large protein complex embedded in the nuclear membrane that facilitates nucleocytoplasmic traffic. The NPC is composed of approximately 30 different proteins: the nucleoporins. The NPC displays 8-fold symmetry and each nucleoporin is present in multiple copies. From the central ring that forms the pore, filaments protrude into the cytoplasm (cytoplasmic filaments) and the nucleoplasm (nuclear basket). Many nucleoporins contain FG repeats which are thought to act as a diffusion barrier and docking sight for transport receptors (karyopherins). Export and import is controlled by the interaction of the karyopherins with localization and export sequences. (Adapted from FBrf0220062 and PMID:21495847).
Protein Function (UniProtKB)
Component of the nuclear pore complex (PubMed:17410542). Has a role in the organization of the inner nuclear membrane proteins at the nuclear envelope (PubMed:22718353). In germ cells, plays a role in the nuclear localization of components of the dpp signaling pathways, such as Medea and phosphorylated Mad (PubMed:21696798). Binds to chromatin, and together with Nup62 and Nup93-1, contributes to karyosome morphology and chromatin organization including attachment to the nuclear envelope in oocytes and nurse cells (PubMed:22718353, PubMed:26341556). Has a role in female fertility including egg chamber development; in nurse cells, has a role in the organization of F-actin in subcortical and cytoplasmic actin filaments important for the transfer of cytoplasm from nurse cells to the growing oocytes (PubMed:10511559, PubMed:17277377, PubMed:17410542, PubMed:9732281). Has a role in male spermatogenesis and fertility (PubMed:10511559, PubMed:9732281). Has a role in germ line cell proliferation (PubMed:21696798).
(UniProt, Q9V463)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Nup154 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9V463)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Antisense UTR: 3' UTR of one isoform overlaps Art8 gene on the opposite strand.

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Nup154-RA
FBtr0089881
NM_057939
4371
1365
Nup154-RC
FBtr0340224
NM_057940
4612
1365
Nup154-RD
FBtr0340225
NM_001298898
5420
1365
Additional Transcript Data and Comments
Reported size (kB)

4.5 (northern blot)

(Kiger et al., 1999)

4.370 (compiled cDNA)

(Gigliotti et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Nup154-PA
FBpp0088820
153.9
1365
6.56
Q9V463
NP_477287
AAF53051
Nup154-PC
FBpp0309199
153.9
1365
6.56
Q9V463
NP_477288
AAF53050
Nup154-PD
FBpp0309200
153.9
1365
6.56
Q9V463
NP_001285827
AHN54341
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1365 aa isoforms: Nup154-PA, Nup154-PC, Nup154-PD
Additional Polypeptide Data and Comments
Reported size (kDa)

1365, 1349 (aa)

(Kiger et al., 1999)

1365 (aa); 154 (kD predicted)

(Gigliotti et al., 1998)
Comments
External Data
Subunit Structure (UniProtKB)

Interacts (via N-terminus) with Nup93-1 (PubMed:22718353). Interacts with Nup35 (PubMed:22718353). Interacts with cup (PubMed:17277377).

(UniProt, Q9V463)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nup154 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.35

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 4
organism | ubiquitous

Comment: reference states 0-2 hr AEL

(Kiger et al., 1999)
embryonic stage 16 -- 17
germline cell
(Kiger et al., 1999)
oogenesis
germarium region 1
(Gigliotti et al., 2000)
germarium region 2
(Gigliotti et al., 2000)
oogenesis stage S10
nurse cell
(Gigliotti et al., 2000)
follicle cell
(Gigliotti et al., 2000)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage & larval stage & adult stage
(Kiger et al., 1999)
embryonic stage 5 -- 9
primordial germ cell | vicinity of
(Kiger et al., 1999)
embryonic stage 8 -- 9
mesoderm
(Kiger et al., 1999)
embryonic stage 11 -- 17
embryonic/larval foregut
(Kiger et al., 1999)
presumptive embryonic/larval central nervous system
(Kiger et al., 1999)
embryonic/larval midgut
(Kiger et al., 1999)
embryonic/larval hindgut
(Kiger et al., 1999)
embryonic stage 14
embryonic brain
(Kiger et al., 1999)
embryonic/larval dorsal vessel
(Kiger et al., 1999)
embryonic stage 16 -- 17
embryonic brain | restricted
(Kiger et al., 1999)
embryonic/larval lymph gland
(Kiger et al., 1999)
larval stage
imaginal disc
(Kiger et al., 1999)
larval brain | restricted
(Kiger et al., 1999)
testis
(Kiger et al., 1999)
adult stage | male
testis
(Kiger et al., 1999)
adult stage & oogenesis
egg chamber
(Kiger et al., 1999)
germarium
(Kiger et al., 1999)
Additional Descriptive Data

Nup154 transcripts are detected at all stages tested on northern blots (embryo, larvae, and adult). They are most abundant in ovaries, 0-2hr and 4-8hr embryos. Transcripts are ubiquitous in 0-2hr embryos. By stage 5, they are localized to the posterior end of the embryo just under the pole cells and this persists through germ band elongation. Transcripts are also detected in the developing mesoderm. At stage 11, Nup154 transcripts are most prominent in the CNS and gut. By stage 14, they are also observed in the brain and dorsal vessel. By the end of embryogenesis, transcripts are restricted to three regions, the lymph glands, the gonadal germline, and several cells like mushroom body neuroblasts in the brain. These areas all contain cells that are mitotically active after the end of embryogenesis. In larvae, overall levels of Nup154 transcripts are low but elevated levels are observed in tissues containing dividing cells, namely imaginal discs and certain regions of the brain. In larval and adult testes, transcripts are present in all male germline cells up through the meiotic stages. In ovaries, staining is observed in germarium region 2 and throughout later stages of oogenesis. Darker staining is observed after stage 8.

(Kiger et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage & oogenesis
spermatocyte
(Gigliotti et al., 1998)
germarium
(Gigliotti et al., 1998)
oocyte
(Gigliotti et al., 1998)
egg chamber
(Gigliotti et al., 1998)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

Nup154 protein is present in the germarium and throughout egg chamber development. It is found both in the soma and the germ line around the nuclear periphery. In stage 10 egg chambers, it is also present in a network inside the nuclei. In the oocyte nucleus, a very bright central dot is observed where the chromosomes are condensed into a central karyosome. Nup154 protein is also localized intranuclearly in spermatocytes and other cell types.

(Gigliotti et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Busayavalasa et al., 2012)
inferred from direct assay
(Riparbelli et al., 2007)
located_in nuclear envelope
inferred from direct assay
(Busayavalasa et al., 2012, Grimaldi et al., 2007)
inferred from direct assay
(Capelson et al., 2010)
located_in nuclear periphery
inferred from direct assay
(Riparbelli et al., 2007, Gigliotti et al., 1998)
part_of nuclear pore
inferred from direct assay
(Riparbelli et al., 2007)
located_in nucleus
inferred from direct assay
(Riparbelli et al., 2007, Gigliotti et al., 1998)
inferred from direct assay
(Busayavalasa et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Nup154 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 18 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Nup154
Transgenic constructs containing regulatory region of Nup154
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(2L)J39
(Kiger et al., 1999, Schmidt et al., 1996)
Df(2L)Prl
(Spradling et al., 1999, BDGP Project Members, 1994-1999)
Inferred from location ( 3 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | recessive
lethal - all die during embryonic stage | maternal effect, with Scer\GAL4VP16.nanos.UTR
lethal - all die during P-stage, with Scer\GAL4Mef2.PR
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female fertile
female semi-sterile | recessive
female sterile
female sterile | recessive
male fertile, with Scer\GAL4VP16.nanos.UTR
male sterile
male sterile | recessive
Other Phenotypes
Allele
abnormal size | third instar larval stage
decreased body size | third instar larval stage
decreased cell number | spermatogenesis (with Nup15401501)
decreased cell number | spermatogenesis (with Nup154RV5)
decreased cell number | third instar larval stage
short lived | heat sensitive, with Scer\GAL4tin.CΔ4
some die during embryonic stage | maternal effect, with Scer\GAL4VP16.nanos.UTR
visible, with Scer\GAL4pnr-MD237
wild-type
Phenotype manifest in
Allele
cyst cell & spindle
cyst cell of testis
dorsal appendage
egg
egg chamber
embryonic/larval hemocyte
embryonic/larval lymph gland
embryonic/larval optic lobe
follicle cell
germline cell | adult stage | male
germline cell | larval stage | male
germline cell | male
imaginal disc
larval brain
meiotic cell cycle | male
mesonotum, with Scer\GAL4pnr-MD237
nurse cell
nurse cell & chromatin
oocyte
ovariole
ovary
spermatid
spermatid axoneme
spermatocyte
spermatocyte & nuclear membrane
testis
testis & meiotic cell cycle
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\NUP155
14 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Nup155
13 of 14
Yes
Yes
Mus musculus (laboratory mouse) (1)
Mmus\Nup155
13 of 14
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\nup155
11 of 13
Yes
Yes
Xtro\grin3a
1 of 13
No
Yes
Danio rerio (Zebrafish) (1)
Drer\nup155
14 of 14
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\npp-8
13 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\AgaP_AGAP007999
12 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\NUP155
13 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\NUP157
12 of 13
Yes
Yes
Scer\NUP170
12 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\nup155
11 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Nup154. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    NUP155; nucleoporin 155
    model of  familial atrial fibrillation
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 1 )
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    NUP155; nucleoporin 155
    14 of 14
    606694
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    Summary of Genetic Interactions
    Interaction Browsers
    View in FlyBase Interactions Browser   View in MIST tool (external link)
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Nup62
    suppressible
    Nup154
    (Breuer and Ohkura, 2015)
    wg
    suppressible
    Nup154
    (McClure and Schubiger, 2008)
    External Data
    Subunit Structure (UniProtKB)
    Interacts (via N-terminus) with Nup93-1 (PubMed:22718353). Interacts with Nup35 (PubMed:22718353). Interacts with cup (PubMed:17277377).
    (UniProt, Q9V463 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Class of Gene
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-44
    Cytogenetic map
    32C1-32C1
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    32C1-32C1
    Limits computationally determined from genome sequence between P{lacW}l(2)k05812k05812&P{lacW}l(2)k09104k09104 and P{EP}ppoEP2478&P{lacW}l(2)k15817k15817
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    32D-32D
    (Fuller, 1998.2.26, Fuller, 1998.2.26, Gigliotti, 1998.4.6)
    32D1-32D2
    (determined by in situ hybridisation)
    (Spradling et al., 1999)
    32D-32D
    (determined by in situ hybridisation)
    (Kiger et al., 1999)
    32C4-32C4
    (determined by in situ hybridisation)
    (Gigliotti et al., 1997)
    32D1-32D2
    (Kiger, 1996.4.29, BDGP Project Members, 1994-1999)
    32C-32D
    (Kiger et al., 1996)
    Experimentally Determined Recombination Data
    Location

    2-44

    (FlyBase, 2016)

    2-37.5

    (Comeron et al., 2012)

    2-

    (Kiger et al., 1996)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (10)
    Genomic Clones (21)
    cDNA Clones (62)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Colozza et al., 2011, Gigliotti et al., 1998)
      Commercially Available Antibodies
       
      Cell Line Information
      Publicly Available Cell Lines
       
        Other Stable Cell Lines
         
          Other Comments

          Nup154 is essential for viability and is required for normal cell proliferation.

          (Kiger et al., 1999)

          Candidate gene for testis length quantitative trait locus.

          (Macdonald and Goldstein, 1999)

          Mutant alleles are male sterile, due to lack of germ-cells in testes.

          (Kiger et al., 1996)

          Nup154, l(2)k07717 and l(2)k08204 are possible mutations of zk.

          (Kiger, 1996.4.29)
          Relationship to Other Genes
          Source for database merge of

          Source for merge of: Nup154 zk

          (Kiger et al., 1999)
          Additional comments
          Nomenclature History
          Source for database identify of

          Source for identity of: Nup154 CG4579

          (Zhong, 1999.11)
          Nomenclature comments
          Etymology
          Synonyms and Secondary IDs (19)
          Reported As
          Symbol Synonym
          BcDNA:LD21772
          CG4579
          (National Institute of Genetics Fly Stocks, 2022-, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Mason and Goldfarb, 2009, Sabri et al., 2007)
          NUP154
          (Raices and D'Angelo, 2012)
          Nup
          (Mensch et al., 2013)
          Nup154
          (Klimkowski Arango and Morgante, 2025, Isaacson et al., 2024, McQuarrie et al., 2023, Sarkar et al., 2023, National Institute of Genetics Fly Stocks, 2022-, Richards et al., 2022, Keegan and Hughes, 2021, Liguori et al., 2021, Okazaki et al., 2020, Riparbelli et al., 2020, Bernard et al., 2018, Camuglia et al., 2018, Crofton et al., 2018, Steyaert et al., 2018, Solis et al., 2017, Boeynaems et al., 2016, Zirin et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Tokusumi et al., 2012, Capelson et al., 2010, Kallappagoudar et al., 2010, Müller et al., 2010, Doumanis et al., 2009, Doheny et al., 2008, McClure and Schubiger, 2008, McElroy et al., 2008, Gabut et al., 2007, Mendjan et al., 2006, Hogarth et al., 2005, Gigliotti et al., 2000)
          Nup155
          (Busayavalasa et al., 2012, Neumann et al., 2010)
          Nup32D
          (Spradling et al., 1999)
          l(2)01501
          (Beaton, 2000.1.31, Mesilaty-Gross et al., 1999)
          l(2)10432
          (Mesilaty-Gross et al., 1999)
          ms(2)zk
          nup154
          (Bonente et al., 2025, Munafò et al., 2021, Hampoelz et al., 2019, Muerdter et al., 2013, Mason and Goldfarb, 2009, Riparbelli et al., 2009, Grimaldi et al., 2007, Gigliotti and Graziani, 2005, Herold et al., 2003, Kiger et al., 1999)
          nup32D
          (Fuller, 1998.2.26, Fuller, 1998.2.26, Fuller, 1998.2.26)
          tip
          (Mason and Goldfarb, 2009)
          tlp
          (Gigliotti et al., 2000, Gigliotti et al., 1998, Gigliotti et al., 1997)
          zk
          (Macdonald and Goldstein, 1999, Kiger and Fuller, 1998, Kiger, 1996.4.29, Kiger and Fuller, 1996, Kiger et al., 1996)
          Name Synonyms
          Nucleoporin 154
          Nucleoporin 154kD
          Nup154
          tulipano
          (Gigliotti et al., 2000, Gigliotti et al., 1998, Gigliotti et al., 1997)
          zonder kloten
          (Kiger, 1996.4.29, Kiger et al., 1996)
          Secondary FlyBase IDs
          • FBgn0010493
          • FBgn0016083
          • FBgn0027862
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (0)
          Result
          Result Type
          Title
          External Crossreferences and Linkouts ( 68 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
          DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
          EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
          FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
          FlyMine - An integrated database for Drosophila genomics
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          MARRVEL_MODEL - MARRVEL (model organism gene)
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          References (108)