zk, tlp, nup32D, tulipano, zonder kloten
Low-frequency RNA-Seq exon junction(s) not annotated.
Antisense UTR: 3' UTR of one isoform overlaps Art8 gene on the opposite strand.
Gene model reviewed during 5.50
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nup154 using the Feature Mapper tool.
Nup154 transcripts are detected at all stages tested on northern blots (embryo, larvae, and adult). They are most abundant in ovaries, 0-2hr and 4-8hr embryos. Transcripts are ubiquitous in 0-2hr embryos. By stage 5, they are localized to the posterior end of the embryo just under the pole cells and this persists through germ band elongation. Transcripts are also detected in the developing mesoderm. At stage 11, Nup154 transcripts are most prominent in the CNS and gut. By stage 14, they are also observed in the brain and dorsal vessel. By the end of embryogenesis, transcripts are restricted to three regions, the lymph glands, the gonadal germline, and several cells like mushroom body neuroblasts in the brain. These areas all contain cells that are mitotically active after the end of embryogenesis. In larvae, overall levels of Nup154 transcripts are low but elevated levels are observed in tissues containing dividing cells, namely imaginal discs and certain regions of the brain. In larval and adult testes, transcripts are present in all male germline cells up through the meiotic stages. In ovaries, staining is observed in germarium region 2 and throughout later stages of oogenesis. Darker staining is observed after stage 8.
Nup154 protein is present in the germarium and throughout egg chamber development. It is found both in the soma and the germ line around the nuclear periphery. In stage 10 egg chambers, it is also present in a network inside the nuclei. In the oocyte nucleus, a very bright central dot is observed where the chromosomes are condensed into a central karyosome. Nup154 protein is also localized intranuclearly in spermatocytes and other cell types.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Nup154 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Nup154 is essential for viability and is required for normal cell proliferation.
Candidate gene for testis length quantitative trait locus.
Mutant alleles are male sterile, due to lack of germ-cells in testes.