FlyBase Gene Report: Dmel\gus

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Gene: Dmel\gus

General Information

Symbol

Dmel\gus

Species

D. melanogaster

Name

gustavus

Annotation Symbol

CG2944

Feature Type

protein_coding_gene

FlyBase ID

FBgn0026238

Gene Model Status

Stock Availability

6 publicly available

Gene Summary

gustavus (gus) encodes a cullin-RING ubiquitin E3 ligase specificity receptor. It interacts genetically and physically with the product of Cul5, and putatively regulates stability of target proteins including the one encoded by vas. It is involved in oogenesis and germ cell maintenance. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

2R:5,116,990..5,128,321 [-]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:5116990..5128321

Sequence

Get Decorated FASTA

Genomic Maps

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (21 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:P09052

(Kugler et al., 2010, Woo et al., 2006, Styhler et al., 2002)

inferred from physical interaction with UniProtKB:Q9VAQ0

(Kugler et al., 2010)

inferred from physical interaction with FLYBASE:vas; FB:FBgn0283442

(Woo et al., 2006)

inferred from physical interaction with UniProtKB:P83940

(Woo et al., 2006)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables ubiquitin-like ligase-substrate adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000956493

(GO Reference Genome Project, 2011-)

Biological Process (11 terms)

Terms Based on Experimental Evidence (9 terms)

CV Term

Evidence

References

involved_in cuticle pattern formation

inferred from mutant phenotype

(Styhler et al., 2002)

involved_in dorsal appendage formation

inferred from mutant phenotype

(Styhler et al., 2002)

involved_in germ cell development

inferred from mutant phenotype

(Kugler et al., 2010)

involved_in germ cell migration

inferred from mutant phenotype

(Styhler et al., 2002)

involved_in intracellular protein localization

inferred from mutant phenotype

(Styhler et al., 2002)

involved_in oocyte anterior/posterior axis specification

inferred from mutant phenotype

(Styhler et al., 2002)

involved_in pole plasm assembly

inferred from mutant phenotype

(Styhler et al., 2002)

involved_in positive regulation of protein catabolic process

inferred from mutant phenotype

(Kugler et al., 2010)

involved_in wing disc morphogenesis

inferred from mutant phenotype

(Kugler et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in intracellular signal transduction

inferred from electronic annotation with InterPro:IPR036036

(InterPro Project Members, 2004-)

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000956493

(GO Reference Genome Project, 2011-)

Cellular Component (8 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Kugler et al., 2010)

part_of Cul5-RING ubiquitin ligase complex

inferred from direct assay

(Kugler et al., 2010)

located_in cytoplasm

inferred from direct assay

(Styhler et al., 2002)

inferred from direct assay

(Kugler et al., 2010)

part_of elongin complex

inferred from direct assay

(Woo et al., 2006)

located_in nucleus

inferred from direct assay

(Kugler et al., 2010)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Kugler et al., 2010, Styhler et al., 2002)

located_in pole plasm

inferred from direct assay

(Kugler et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of SCF ubiquitin ligase complex

inferred from biological aspect of ancestor with PANTHER:PTN001387175

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Protein Family (UniProt)

Belongs to the SPSB family. (A1Z6E0)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

gustavus (gus) encodes a cullin-RING ubiquitin E3 ligase specificity receptor. It interacts genetically and physically with the product of Cul5, and putatively regulates stability of target proteins including the one encoded by vas. It is involved in oogenesis and germ cell maintenance. [Date last reviewed: 2019-03-07]

Protein Function (UniProtKB)

Involved in the localization of vas to the posterior pole of the oocyte. Required maternally in the germ line for efficient primordial germ cell formation.

(UniProt, A1Z6E0)

Gene Model and Products

Number of Transcripts

7

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\gus for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A1Z6E0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

PDB - An information portal to biological macromolecular structures

2FNJ
2IHS

Comments on Gene Model

Gene model reviewed during 5.41

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0309846

1745

279

FBtr0309847

1395

279

FBtr0309848

2040

279

FBtr0309849

1730

279

FBtr0309850

1813

279

FBtr0309851

1933

279

FBtr0309852

1525

279

Additional Transcript Data and Comments

Reported size (kB)

1.6 (northern blot)

(Styhler et al., 2002)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

FBpp0301580

31.7

279

9.31

FBpp0301581

31.7

279

9.31

FBpp0301582

31.7

279

9.31

FBpp0301583

31.7

279

9.31

FBpp0301584

31.7

279

9.31

FBpp0301585

31.7

279

9.31

FBpp0301586

31.7

279

9.31

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

279 aa isoforms: gus-PG, gus-PH, gus-PI, gus-PJ, gus-PK, gus-PL, gus-PM

Additional Polypeptide Data and Comments

Reported size (kDa)

281 (aa); 32 (kD)

(Styhler et al., 2002)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts (via B30.2/SPRY domain) with vas; this interaction may be necessary for the transport of vas to the posterior pole of the oocyte. Interacts with Cul-5. May associate with the Elongin BC complex composed of Elongin-B and Elongin-C.

(UniProt, A1Z6E0)

Crossreferences

InterPro - A database of protein families, domains and functional sites

PDB - An information portal to biological macromolecular structures

2FNJ
2IHS

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\gus using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

gus_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.60

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 9

Comment: reference states 0-4 hr AEL

(Styhler et al., 2002)

adult stage | female

(Styhler et al., 2002)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

(Kugler et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

germarium & nurse cell

• cytoplasm

(Styhler et al., 2002)

adult stage oogenesis stage S6 -- S9

• cytoplasm

(Styhler et al., 2002)

oogenesis stage S10

• cytoplasm

(Styhler et al., 2002)

oogenesis stage S10A

• cytoplasm

(Styhler et al., 2002)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

(Kugler et al., 2010)

Additional Descriptive Data

A band corresponding to the 281aa gus protein is observed on western blots. No sign of a possible longer isoform (translated from an upstream AUG) was observed.

(Kugler et al., 2010)

gus protein can be detected in the cytoplasm of nurse cells during the germarial stage and by stage 3 of oogenesis is punctate in the cytoplasm. gus protein also accumulates in the cytoplasm of the oocyte from stages 6-9. At stage 10 there is a transient accumulation of the protein at the posterior pole. However, in later stages of oogenesis and in early embryos there is the protein is distributed throughout the oocyte cytoplasm.

(Styhler et al., 2002)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cell cortex

inferred from direct assay

(Kugler et al., 2010)

part_of Cul5-RING ubiquitin ligase complex

inferred from direct assay

(Kugler et al., 2010)

located_in cytoplasm

inferred from direct assay

(Styhler et al., 2002)

inferred from direct assay

(Kugler et al., 2010)

part_of elongin complex

inferred from direct assay

(Woo et al., 2006)

located_in nucleus

inferred from direct assay

(Kugler et al., 2010)

located_in perinuclear region of cytoplasm

inferred from direct assay

(Kugler et al., 2010, Styhler et al., 2002)

located_in pole plasm

inferred from direct assay

(Kugler et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\gus in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\gus

View exonic expression by tissue for Dmel\gus

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0026238

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35478/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0026238

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0026238

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0026238

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2944

Flygut - An atlas of the Drosophila adult midgut

FBgn0026238

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of gus

Transgenic constructs containing regulatory region of gus

Aberrations (Deficiencies and Duplications) ( 6 )

Inferred from experimentation ( 6 )

Gene disrupted in

(Myster et al., 2004)

(Myster et al., 2004)

(Kugler et al., 2010)

(Styhler et al., 2002)

(Myster et al., 2004)

Gene not disrupted in

(Myster et al., 2004)

Inferred from location ( 1 )

Df(2R)BSC630

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage, with Scer\GAL4^da.G32

gus^GD3725

lethal - all die during embryonic stage | maternal effect, with gus^f07073

gus^UAS.L.Tag:HA

lethal - all die during embryonic stage | maternal effect, with gus^UAS.L.Tag:HA

gus^f07073

partially lethal - majority live | maternal effect (with Df(2R)nap1)

gus^f04367

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

gus^GD3725

viable, with Scer\GAL4^Mef2.PR

gus^GD3725

viable, with Scer\GAL4^pnr-MD237

gus^GD3725

viable, with Scer\GAL4^tin.CΔ4

gus^GD3725

Sterility

Allele

gus^Z409

female sterile (with Df(2R)nap14)

gus^Z409

gus^f04367

Other Phenotypes

Allele

some die during embryonic stage, with Scer\GAL4^da.G32

gus^GD3725

visible (with Df(2R)nap1)

Phenotype manifest in

Allele

abdominal ventral denticle belt | maternal effect

gus^Z409

dorsal appendage

gus^Z409

egg | maternal effect

gus^f07073

egg | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}

egg | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, gus^f07073

gus^UAS.S.Tag:HA

egg | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, gus^UAS.S.Tag:HA

gus^f07073

gus^Z409

embryo | maternal effect

embryo | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}

embryo | maternal effect (with Df(2R)nap1)

gus^f07073

gus^Z409

oocyte | oogenesis stage S8

gus^Z409

oocyte | oogenesis stage S9

gus^Z409

gus^Z409

primordial germ cell | maternal effect

gus^f07073

primordial germ cell | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}

wing blade (with Df(2R)nap1)

wing margin | ectopic (with Df(2R)nap1)

wing vein (with Df(2R)nap1)

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

14 of 14

Yes

Yes

13 of 14

No

Yes

4 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (21)

14 of 14

Yes

Yes

13 of 14

No

Yes

4 of 14

No

Yes

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

Mus musculus (laboratory mouse) (23)

14 of 14

Yes

Yes

12 of 14

No

Yes

4 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

Xenopus tropicalis (Western clawed frog) (13)

11 of 13

Yes

Yes

8 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

Yes

1 of 13

No

Yes

1 of 13

No

No

1 of 13

No

No

Danio rerio (Zebrafish) (42)

14 of 14

Yes

Yes

13 of 14

No

Yes

9 of 14

No

Yes

Drer\si:ch1073-228j22.2

3 of 14

No

Yes

2 of 14

No

No

2 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Drer\si:ch73-54f23.4

1 of 14

No

Yes

Drer\si:ch73-106l15.3

1 of 14

No

Yes

Drer\si:ch211-107m4.1

1 of 14

No

No

Drer\si:ch211-114c12.5

1 of 14

No

Yes

Drer\si:dkey-23n7.10

1 of 14

No

No

Drer\si:dkey-219e21.2

1 of 14

No

Yes

Drer\si:dkey-264f17.2

1 of 14

No

Yes

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

Yes

1 of 14

No

Yes

1 of 14

No

No

Caenorhabditis elegans (Nematode, roundworm) (6)

13 of 14

Yes

Yes

9 of 14

No

Yes

3 of 14

No

No

1 of 14

No

No

1 of 14

No

No

1 of 14

No

No

Anopheles gambiae (African malaria mosquito) (6)

Agam\AgaP_AGAP010197

12 of 12

Yes

Yes

Agam\AgaP_AGAP009203

2 of 12

No

No

Agam\FBSP1_ANOGA

2 of 12

No

No

Agam\AgaP_AGAP004765

1 of 12

No

No

Agam\AgaP_AGAP005063

1 of 12

No

No

Agam\AgaP_AGAP008750

1 of 12

No

No

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPCC1259.12c

1 of 12

Yes

No

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:gus. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (5)

4 of 13

4 of 13

4 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SPSB4; splA/ryanodine receptor domain and SOCS box containing 4

14 of 14

SPSB1; splA/ryanodine receptor domain and SOCS box containing 1

13 of 14

SPSB2; splA/ryanodine receptor domain and SOCS box containing 2

4 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

anti tag coimmunoprecipitation, western blot

(Kugler et al., 2010)

experimental knowledge based

(Guruharsha et al., 2011)

pull down, autoradiography

(Lunardi et al., 2010)

(Battersby et al., 2003)

two hybrid, pull down, molecular weight estimation by staining, isothermal titration calorimetry, predetermined participant, anti bait coimmunoprecipitation, western blot, x-ray crystallography, anti tag western blot

(Dehghani and Lasko, 2016, Kugler et al., 2010, Woo et al., 2006, Woo et al., 2006, Styhler et al., 2002)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Kugler et al., 2010)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Interacts (via B30.2/SPRY domain) with vas; this interaction may be necessary for the transport of vas to the posterior pole of the oocyte. Interacts with Cul-5. May associate with the Elongin BC complex composed of Elongin-B and Elongin-C.

(UniProt, A1Z6E0 )

Linkouts

BioGRID - A database of protein and genetic interactions.

61396

DroID - A comprehensive database of gene and protein interactions.

FBgn0026238

MIST (protein-protein) - An integrated Molecular Interaction Database

35478

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

2R

Recombination map

2-55

Cytogenetic map

Sequence location

2R:5,116,990..5,128,321 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

41D3-41D3

Limits computationally determined from genome sequence between P{PZ}vlc⁰⁷⁰²²&P{lacW}l(2)09851^k08138 and P{PZ}l(2)09851⁰⁹⁸⁵¹&P{lacW}Src42A^k10108

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-55.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (6)

Aberrations including deletions of this gene Aberrations including duplications of this gene

18764

w¹¹¹⁸; PBac{WH}gus^f04367

606544

y¹ w^*; TI{CRIMIC.TG4.1}gus^{CR01562-TG4.1}/SM6a

66321

y¹ sc^* v¹ sev²¹; P{TRiP.HMC06209}attP2

2RG-1050

y² VhaAC39-1^cho-1 v¹; P{U6-gus.NIG.sgRNA}attP40/CyO

v101738

P{KK108241}VIE-260B

v8688

w¹¹¹⁸; P{GD3725}v8688

Genomic Clones (24)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (122)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_LD34464

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0026238

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0026238

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Kugler et al., 2010, Styhler et al., 2002)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

gus binds with 1:1 stoichiometry to vas.

(Woo et al., 2006)

gus is present in cytoplasmic, ribonucleo-protein particles, and is required for localization vas protein in the oocytes.

(Styhler et al., 2002)

gus mutants have defects in anterior-posterior patterning of the eggshell, and embryo (maternal effect).

(Styhler et al., 2002)

Identification: Defined in yeast two hybrid system using vas as a bait.

(Styhler et al., 1999)

Relationship to Other Genes

Source for database merge of

Source for merge of: CG2944 gus

(Styhler et al., 2002)

Source for merge of: CG2944 CG2946

(FlyBase, 1996-)

Additional comments

Source for merge of CG2944 CG2946 was sequence comparison ( date:001104 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

This gene is named "gustavus" ("gus") since that was King Vasa's first name, and the gus gene product interacts with that of vas.

(Lasko, 1999.3.4)

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

A11

(Styhler et al., 1999)

CG2944

(National Institute of Genetics Fly Stocks, 2022-, Cho et al., 2020, Caizzi et al., 2016, Keleman et al., 2009.8.5, Perkins et al., 2009, Rossi et al., 2007, Brandt, 2006, Woo et al., 2006, Dimitri et al., 2005, Marygold et al., 2005, Myster et al., 2004, Stanyon et al., 2004, Battersby et al., 2003, Corradini et al., 2003, Giot et al., 2003, Styhler et al., 2002)

CG2946

GUS

(Woo et al., 2006)

Gus

(Sankaranarayanan and Weil, 2020, Kugler and Lasko, 2007)

dmSSB4

(Kile et al., 2002)

gus

(Pavlidaki et al., 2022, Gene Disruption Project members, 2018-, Kline et al., 2017, Ashwal-Fluss et al., 2014, Toshima et al., 2014, Rodriguez et al., 2012, Di Stefano et al., 2011, Friedman et al., 2011, Coulthard et al., 2010, Kugler et al., 2010, Kugler et al., 2010, Jiang et al., 2009, Kugler et al., 2008, Brandt, 2006)

i25

(Battersby et al., 2003)

Name Synonyms

GUSTAVUS

(Woo et al., 2006)

Gustavus

(Kugler et al., 2013, Kugler et al., 2010, Kugler et al., 2008, Kugler and Lasko, 2007)

gustavus

(Kugler et al., 2010, Kugler et al., 2010, Lunardi et al., 2010, Woo et al., 2006, Lasko, 1999.3.4)

Secondary FlyBase IDs

FBan0002944
FBgn0033008
FBgn0033009

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 71 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35478

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A1Z6E0

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A1Z6E0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4K6X3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A1Z6E0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0026238

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35478/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0026238

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0026238

FlyMine - An integrated database for Drosophila genomics

FBgn0026238

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2944

MARRVEL_MODEL - MARRVEL (model organism gene)

35478

PDB - An information portal to biological macromolecular structures

2FNJ
2IHS

Linkouts

BioGRID - A database of protein and genetic interactions.

61396

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0026238

DroID - A comprehensive database of gene and protein interactions.

FBgn0026238

DRSC - Results frm RNAi screens

FBgn0026238

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

gus_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0026238

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0026238

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2944

Flygut - An atlas of the Drosophila adult midgut

FBgn0026238

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0026238

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034075

MIST (protein-protein) - An integrated Molecular Interaction Database

35478

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (67)