FlyBase Gene Report: Dmel\Mtpα

General Information

Symbol

Dmel\Mtpα

Species

D. melanogaster

Name

Mitochondrial trifunctional protein α subunit

Annotation Symbol

CG4389

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028479

Gene Model Status

Current

Stock Availability

10 publicly available

Enzyme Name (EC)

long-chain-3-hydroxyacyl-CoA dehydrogenase (1.1.1.211)

3-hydroxyacyl-CoA dehydrogenase (1.1.1.35)

enoyl-CoA hydratase (4.2.1.17)

Gene Summary

Mitochondrial trifunctional protein α subunit (Mtpα) encodes a subunit of the mitochondrial trifunctional protein. It possesses 3-enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase activities, which catalyze the second and third steps,respectively, of the β-oxidation of long-chain fatty acids. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

BcDNA:GH12558

Key Links

Genomic Location

Cytogenetic map

30B12-30B12

Sequence location

2L:9,573,563..9,576,769 [-]

Recombination map

2-35

RefSeq locus

NT_033779 REGION:9573563..9576769

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (7 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (7 terms)

CV Term

Evidence

References

enables (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity

inferred from electronic annotation with InterPro:IPR012803

(InterPro Project Members, 2004-)

enables catalytic activity

inferred from electronic annotation with InterPro:IPR018376

(InterPro Project Members, 2004-)

enables enoyl-CoA hydratase activity

inferred from biological aspect of ancestor with PANTHER:PTN000592895

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR012803

(InterPro Project Members, 2004-)

inferred from sequence or structural similarity with UniProtKB:Q64428

(Gene Ontology Curators, 2002-)

enables long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity

inferred from sequence or structural similarity with UniProtKB:Q64428

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000592895

(GO Reference Genome Project, 2011-)

enables NAD+ binding

inferred from electronic annotation with InterPro:IPR006176

(InterPro Project Members, 2004-)

enables oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

inferred from electronic annotation with InterPro:IPR006108

(InterPro Project Members, 2004-)

enables oxidoreductase activity

inferred from electronic annotation with InterPro:IPR006108

(InterPro Project Members, 2004-)

Biological Process (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in determination of adult lifespan

inferred from mutant phenotype

(Kishita et al., 2012)

involved_in fatty acid beta-oxidation

inferred from mutant phenotype

(Kishita et al., 2012)

involved_in response to starvation

inferred from mutant phenotype

(Kishita et al., 2012)

involved_in wound healing

inferred from high throughput mutant phenotype

(Campos et al., 2010)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in fatty acid beta-oxidation

inferred from electronic annotation with InterPro:IPR012803

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000592895

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with UniProtKB:Q64428

(Gene Ontology Curators, 2002-)

involved_in fatty acid metabolic process

inferred from electronic annotation with InterPro:IPR006108, InterPro:IPR006176

(InterPro Project Members, 2004-)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

located_in peroxisome

inferred from direct assay

(Faust et al., 2012)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

part_of mitochondrial fatty acid beta-oxidation multienzyme complex

inferred from electronic annotation with InterPro:IPR012803

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000592780

(GO Reference Genome Project, 2011-)

is_active_in mitochondrial inner membrane

inferred from sequence or structural similarity with UniProtKB:P40939

(Gene Ontology Curators, 2002-)

located_in mitochondrion

inferred from electronic annotation with InterPro:IPR012803

(InterPro Project Members, 2004-)

inferred from sequence model

(Faust et al., 2012)

located_in peroxisome

inferred from sequence or structural similarity with UniProtKB:Q08426

(Faust et al., 2012)

inferred from sequence model

(Faust et al., 2012)

Gene Group (FlyBase)

MITOCHONDRIAL TRIFUNCTIONAL PROTEIN COMPLEX

ENOYL-COA HYDRATASES

UNCLASSIFIED CH-OH OXIDOREDUCTASES, NAD OR NADP AS ACCEPTOR

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity

a long-chain (3S)-3-hydroxy fatty acyl-CoA + NAD(+) = a long-chain 3-oxo- fatty acyl-CoA + NADH + H(+) (1.1.1.211)

RHEA 52656: (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity

a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + NADH + H(+) (1.1.1.35)

RHEA 22432: enoyl-CoA hydratase activity

(1) a 4-saturated-(3S)-3-hydroxyacyl-CoA = a (3E)-enoyl-CoA + H2O (4.2.1.17)

(2) a (3S)-3-hydroxyacyl-CoA = a (2E)-enoyl-CoA + H2O (4.2.1.17)

oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Summaries

Gene Snapshot

Gene Group (FlyBase)

MITOCHONDRIAL TRIFUNCTIONAL PROTEIN COMPLEX -: Mitochondrial trifunctional protein (MTP) catalyzes the latter three steps (hydration, oxidation and thiolysis) in the beta-oxidation of long-chain fatty acids. MTP consists of a hetero-octamer of four MTP-alpha and four MTP-beta subunits. The MTP-alpha subunit possesses long-chain 3-enoyl-CoA hydratase and long-chain 3-hydroxyacyl-CoA dehydrogenase activities, which catalyze the second and third steps, respectively. The MTP-beta subunit has long-chain 3-ketoacyl-CoA thiolase activity, which catalyzes the fourth step. Mitochondrial beta-oxidation is the major metabolic system to catabolize fatty acids, yielding the primary energy source for various cellular processes. (Adapted from FBrf0217677.)
ENOYL-COA HYDRATASES -: Enoyl-CoA hydratases catalyse the hydration of an enoyl-CoA molecule to form a 3-hydroxyacyl-CoA, or the reverse dehydratase reaction.
UNCLASSIFIED CH-OH OXIDOREDUCTASES, NAD OR NADP AS ACCEPTOR -: This group comprises CH-OH oxidoreductases using NAD or NADP as acceptor that do not classify under other groups in FlyBase.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Mtpα for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V397)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mtpα-RA

FBtr0079858

NM_135455

2725

783

Mtpα-RB

FBtr0079857

NM_164860

2886

744

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Mtpα-PA

FBpp0079454

84.1

783

9.72

FBpp0079453

79.6

744

9.31

Q8IPE8

NP_723470

AAN10697

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mtpα using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.20

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval lymph gland

(Tiwari et al., 2020)

Additional Descriptive Data

Transcripts were detected in the late third instar lymph gland.

(Tiwari et al., 2020)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 5 of adulthood | male -- day 7 of adulthood | male

spermatozoon

(Wasbrough et al., 2010)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

located_in peroxisome

inferred from direct assay

(Faust et al., 2012)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Mtpα in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mtpα

View exonic expression by tissue for Dmel\Mtpα

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028479

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34276/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028479

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028479

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028479

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4389

Flygut - An atlas of the Drosophila adult midgut

FBgn0028479

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mtpα

Transgenic constructs containing regulatory region of Mtpα

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2L)Exel7042

(Ryder, 2004.4.26)

Inferred from location ( 9 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Mtpα^KO

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Mtpα^GD11299

viable, with Scer\GAL4^Mef2.PR

Mtpα^GD11299

viable, with Scer\GAL4^pnr-MD237

Mtpα^GD11299

viable, with Scer\GAL4^tin.CΔ4

Mtpα^GD11299

Sterility

Allele

fertile

Mtpα^KO

male fertile, with Scer\GAL4^bam.PU

Mtpα^HMS00660

Other Phenotypes

Allele

abnormal cell number | larval stage

Mtpα^KO

abnormal locomotor behavior | adult stage

Mtpα^KO

abnormal starvation stress response | adult stage

Mtpα^KO

short lived

Mtpα^KO

Phenotype manifest in

Allele

adult fat body

Mtpα^KO

lipid droplet

Mtpα^KO

primary lobe of embryonic/larval lymph gland | larval stage

Mtpα^KO

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\HADHA

14 of 14

Yes

Hsap\EHHADH

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (16)

Rnor\Hadha

14 of 14

Yes

Rnor\Ehhadh

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (16)

Mmus\Hadha

13 of 14

Yes

Mmus\Ehhadh

6 of 14

Yes

Mmus\Echdc1

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (13)

Xtro\hadha

8 of 13

Yes

Xtro\ehhadh

4 of 13

Yes

Xtro\cdyl2

1 of 13

Xtro\cdyl

1 of 13

Xtro\cyp3a5

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (13)

Drer\hadhaa

14 of 14

Yes

Drer\hadhab

14 of 14

Yes

Drer\ehhadh

6 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (19)

Cele\ech-1.1

14 of 14

Yes

Cele\ech-1.2

13 of 14

Yes

Cele\ech-8

3 of 14

Yes

Cele\ech-9

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (13)

Agam\AgaP_AGAP007784

11 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (15)

Atha\AIM1

9 of 13

Yes

Atha\MFP2

9 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\DCI1

2 of 13

Yes

Scer\EHD3

2 of 13

Yes

Scer\BNA4

1 of 13

Scer\ECI1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC2D10.09

2 of 12

Yes

Escherichia coli (enterobacterium) (8)

Ecol\fadJ

10 of 11

Yes

Ecol\fadB

4 of 11

Yes

2 of 11

2 of 11

1 of 11

1 of 11

1 of 11

1 of 11

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Mtpα. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (15)

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Dci

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

mitochondrial trifunctional protein deficiency 1

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Allele

Disease

Evidence

References

Mtpα^KO

model of steatotic liver disease

CEA

(Kishita et al., 2012)

model of lipid metabolism disorder

CEA

(Kishita et al., 2012)

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

HADHA; hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha

model of long chain 3-hydroxyacyl-CoA dehydrogenase deficiency

IEA

(FlyBase, 2019-)

model of mitochondrial trifunctional protein deficiency 1

IEA

(FlyBase, 2019-)

EHHADH; enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase

model of Fanconi renotubular syndrome 3

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HADHA; hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha

14 of 14

HYDROXYACYL-CoA DEHYDROGENASE/3-KETOACYL-CoA THIOLASE/ENOYL-CoA HYDRATASE, ALPHA SUBUNIT; HADHA

EHHADH; enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase

6 of 14

607037

FANCONI RENOTUBULAR SYNDROME 3; FRTS3

Fanconi renotubular syndrome 3

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Mtpα - CG7407

experimental knowledge based

(Guruharsha et al., 2011)

Mtpα - Mtpβ

experimental knowledge based

(Guruharsha et al., 2011)

Mtpα - ds

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Mtpα - fj

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Mtpα - ft

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Mtpα - su(Hw)

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Mtpα - wts

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Cln3

enhanceable

Mtpα

(Llavero Hurtado et al., 2017)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60375

DroID - A comprehensive database of gene and protein interactions.

FBgn0028479

MIST (protein-protein) - An integrated Molecular Interaction Database

34276

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-35

Cytogenetic map

30B12-30B12

Sequence location

2L:9,573,563..9,576,769 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

30B12-30B12

Limits computationally determined from genome sequence between P{EP}Oatp30B^EP890 and P{EP}EP361&P{EP}pelo^EP2160

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-35

(FlyBase, 2016)

2-30.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

18373

w¹¹¹⁸; PBac{WH}Mtpα^f00822/CyO

77201

y¹ sc^* v¹ sev²¹; P{TKO.GS00727}attP40

32873

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00660}attP2

NIG-Fly

M2L-3258

y¹ w¹¹¹⁸; Mtpα^SK14 P{neoFRT}40A/CyO-Tb

M2L-3259

y¹ w¹¹¹⁸; Mtpα^SK18 P{neoFRT}40A/CyO-Tb

HMS00660

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00660}attP2

2LG-1448

y² VhaAC39-1^cho-1 v¹; P{U6-Mtpα.NIG.sgRNA.1}attP40/CyO

2LG-3167

y² VhaAC39-1^cho-1 v¹; P{U6-Mtpα.NIG.sgRNA.2}attP40/CyO

VDRC

v100021

P{KK102959}VIE-260B

v21845

w¹¹¹⁸; P{GD11299}v21845

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (105)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH12558

Other clones

DPiM_GH12558

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0028479

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0028479

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Additional comments

It is not clear whether or not " NEST:bs09e08 " and " BcDNA:GH12558 " represent a single gene or two neighbouring genes transcribed in the same direction.

(Andrews et al., 2000)

Nomenclature History

Source for database identify of

Source for identity of: Mtpα CG4389

(Kishita et al., 2012)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

BcDNA:GH12558

(Giot et al., 2003)

CG 4389

(Alonso et al., 2005)

HADHA

(Baron et al., 2016)

Hadha

(Llavero Hurtado et al., 2017)

LBP

(Anderson-Baron and Simmonds, 2018)

MTP

(Li et al., 2024)

Mtp α

(Choo et al., 2015)

Mtpalpha

(Stobdan et al., 2019, Marygold, 2018.6.26, Laflamme et al., 2017, Paiardi et al., 2017, Transgenic RNAi Project members, 2017-, Kwon et al., 2013)

Mtpα

(Geronazzo et al., 2025, Haas et al., 2025, Gera et al., 2024, Lee et al., 2024, François et al., 2023, McMullen et al., 2023, Eickelberg et al., 2022, Zipper et al., 2022, Pridie et al., 2020, Hughes and Simmonds, 2019, Anderson-Baron and Simmonds, 2018, O'Sullivan et al., 2013, Kishita et al., 2012)

Name Synonyms

Mitochondrial trifunctional protein α subunit

(Anderson-Baron and Simmonds, 2018)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

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Title

External Crossreferences and Linkouts ( 86 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34276

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V397

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V397

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028479

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

34276/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028479

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028479

FlyMine - An integrated database for Drosophila genomics

FBgn0028479

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4389

MARRVEL_MODEL - MARRVEL (model organism gene)

34276

Linkouts

BioGRID - A database of protein and genetic interactions.

60375

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0028479

DroID - A comprehensive database of gene and protein interactions.

FBgn0028479

DRSC - Results frm RNAi screens

FBgn0028479

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028479

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0028479

FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4389

Flygut - An atlas of the Drosophila adult midgut

FBgn0028479

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0028479

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014680

KEGG Metabolic Pathways - A collection of manually drawn metabolic pathway maps representing knowledge of molecular interaction, reaction and relation networks.

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

MIST (protein-protein) - An integrated Molecular Interaction Database

34276

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Reactome Metabolic Pathways - An open-source, open access, manually curated and peer-reviewed metabolic pathway database.

References (105)

The following external sites may use different assemblies or annotations than FlyBase.

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