FlyBase Gene Report: Dmel\Sec23

General Information

Symbol

Dmel\Sec23

Species

D. melanogaster

Name

Secretory 23

Annotation Symbol

CG1250

Feature Type

protein_coding_gene

FlyBase ID

FBgn0262125

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Secretory 23 (Sec23) encodes a COPII vesicle-coating component that functions in vesicle transport from the ER to the Golgi apparatus. It is involved in cell polarity maintenance and extracellular matrix secretion. [Date last reviewed: 2019-08-01] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Red Book
Interactive Fly

Also Known As

dSec23, dSec23p, haunted, COPII, hau

Key Links

Genomic Location

Cytogenetic map

83B7-83B8

Sequence location

3R:5,649,065..5,652,629 [+]

Recombination map

3-47.5

RefSeq locus

NT_033777 REGION:5649065..5652629

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (22 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables GTPase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN000124617

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with SGD:S000006385

(Gene Ontology Curators, 2002-)

enables zinc ion binding

inferred from electronic annotation with InterPro:IPR006895, InterPro:IPR036174

(InterPro Project Members, 2004-)

Biological Process (15 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

acts_upstream_of_positive_effect chitin-based cuticle development

inferred from mutant phenotype

(Norum et al., 2010)

involved_in chitin-based larval cuticle pattern formation

inferred from mutant phenotype

(Abrams and Andrew, 2005)

involved_in endoplasmic reticulum to Golgi vesicle-mediated transport

inferred from mutant phenotype

(Norum et al., 2010)

involved_in extracellular matrix constituent secretion

inferred from mutant phenotype

(Norum et al., 2010)

involved_in Golgi organization

inferred from genetic interaction with FLYBASE:ecd; FB:FBgn0000543

(Kondylis et al., 2001)

involved_in larval chitin-based cuticle development

inferred from mutant phenotype

(Abrams and Andrew, 2005)

acts_upstream_of_positive_effect lumen formation, open tracheal system

inferred from mutant phenotype

(Norum et al., 2010)

involved_in positive regulation of dendrite morphogenesis

inferred from mutant phenotype

(Ye et al., 2007)

involved_in protein localization to cell surface

inferred from mutant phenotype

(Norum et al., 2010)

involved_in protein localization to plasma membrane

inferred from mutant phenotype

(Kumichel et al., 2015)

involved_in protein secretion

inferred from mutant phenotype

(Norum et al., 2010)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in COPII-coated vesicle budding

inferred from electronic annotation with InterPro:IPR037550

(InterPro Project Members, 2004-)

involved_in COPII-coated vesicle cargo loading

inferred from biological aspect of ancestor with PANTHER:PTN000124617

(GO Reference Genome Project, 2011-)

involved_in endoplasmic reticulum to Golgi vesicle-mediated transport

inferred from electronic annotation with InterPro:IPR006895, InterPro:IPR006896, InterPro:IPR006900, InterPro:IPR036174, InterPro:IPR036175

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR006896

(InterPro Project Members, 2004-)

involved_in intracellular protein transport

inferred from electronic annotation with InterPro:IPR006895, InterPro:IPR006896, InterPro:IPR006900, InterPro:IPR036174, InterPro:IPR036175

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR006896

(InterPro Project Members, 2004-)

involved_in regulation of COPII vesicle coating

inferred from sequence or structural similarity with SGD:S000006385

(Gene Ontology Curators, 2002-)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

part_of COPII vesicle coat

inferred from mutant phenotype

(Norum et al., 2010)

located_in endoplasmic reticulum exit site

inferred from direct assay

(Ivan et al., 2008, Herpers and Rabouille, 2004)

located_in endoplasmic reticulum

inferred from direct assay

(Kondylis et al., 2001)

located_in Golgi stack

inferred from direct assay

(Kondylis et al., 2001)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of COPII vesicle coat

inferred from electronic annotation with InterPro:IPR006895, InterPro:IPR006896, InterPro:IPR006900, InterPro:IPR036174, InterPro:IPR036175, InterPro:IPR037550

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR006896

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000124617

(GO Reference Genome Project, 2011-)

inferred from sequence or structural similarity with SGD:S000006385

(Gene Ontology Curators, 2002-)

located_in COPII-coated ER to Golgi transport vesicle

traceable author statement

(Kondylis et al., 2001)

is_active_in endoplasmic reticulum exit site

inferred from biological aspect of ancestor with PANTHER:PTN000124617

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

COAT PROTEIN COMPLEX II

ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

COAT PROTEIN COMPLEX II -: The Coat Protein Complex II (COPII) mediates protein transport from the ER to Golgi (anterograde transport). COPII recruitment is initiated by the activation of the small GTPase Sar1. Sar1 recruits a heterodimeric complex comprising of Sec23/24, which interacts with cargo and the outer cage - a heterotetramer composed of Sec13/31. (Adapted from PMID:18060556).
ADP-RIBOSYLATION FACTOR GTPASE ACTIVATING PROTEINS -: ArfGAPs are GTPase activating proteins for ARF family GTPases. ARFGAPs contain a characteristic 130 amino acid module, the ArfGAP domain, originally defined as the minimal fragment possessing ArfGAP activity. (Adapted from FBrf0158741, PMID:15731001 and PMID:18809720).

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

hau: haunted

Homozygous lethal; only head skeleton visible in homozygous embryo; no differentiation of cuticle.

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Sec23 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0B4K5Z8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.40

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.49

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Sec23-RA

FBtr0078711

NM_169088

2706

773

Sec23-RB

FBtr0078710

NM_169089

2798

773

Sec23-RD

FBtr0304794

NM_001260005

2819

780

Sec23-RE

FBtr0309091

NM_001260003

2701

781

Sec23-RF

FBtr0310266

NM_001260004

2698

780

Sec23-RG

FBtr0330314

NM_001275365

2590

773

Sec23-RH

FBtr0339320

NM_001300255

2677

773

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Sec23-PA

FBpp0078360

86.8

773

6.78

FBpp0078359

86.8

773

6.78

FBpp0293334

87.5

780

6.78

FBpp0301099

87.6

781

6.78

FBpp0301949

87.5

780

6.78

FBpp0303346

86.8

773

6.78

FBpp0308424

86.8

773

6.78

Q9VNF8

NP_001287184

AHN57183

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

780 aa isoforms: Sec23-PD, Sec23-PF

773 aa isoforms: Sec23-PA, Sec23-PB, Sec23-PG, Sec23-PH

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Sec23 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sec23_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.02

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

yolk

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

head mesoderm

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

foregut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

proventriculus primordium

(Fisher et al., 2012)

salivary gland body primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of COPII vesicle coat

inferred from mutant phenotype

(Norum et al., 2010)

located_in endoplasmic reticulum exit site

inferred from direct assay

(Ivan et al., 2008, Herpers and Rabouille, 2004)

located_in endoplasmic reticulum

inferred from direct assay

(Kondylis et al., 2001)

located_in Golgi stack

inferred from direct assay

(Kondylis et al., 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Sec23 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Sec23

View exonic expression by tissue for Dmel\Sec23

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262125

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40694/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262125

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037357

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262125

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1250

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Sec23

Transgenic constructs containing regulatory region of Sec23

Aberrations (Deficiencies and Duplications) ( 4 )

Inferred from experimentation ( 4 )

Gene disrupted in

Df(3R)by10

(Giesen, 2001.1.24)

Df(3R)ED5187

(Norum et al., 2010)

Df(3R)Exel7283

(Ryder, 2004.4.26)

Gene not disrupted in

Df(3R)p-XT103

(Giesen, 2001.1.24)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Sec23^GD7746

lethal, with Scer\GAL4^pnr-MD237

Sec23^GD7746

lethal - all die before end of first instar larval stage

lethal - all die before end of first instar larval stage (with Df(3R)ED5187)

lethal - all die before end of first instar larval stage (with Sec23^9G)

Sec23^CK

lethal - all die before end of first instar larval stage (with Sec23^CK)

Sec23^9G

lethal - all die during embryonic stage | recessive

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

Sec23^GD7746

viable

Sec23^EY06757

viable (with Df(3R)ED5187)

Sec23^j13C8

viable (with Sec23^9G)

Sec23^j13C8

viable (with Sec23^j13C8)

Sec23^9G

Sterility

Allele

fertile

Sec23^EY06757

Other Phenotypes

Allele

abnormal cell adhesion | first instar larval stage

Sec23^9G

abnormal cell polarity | late embryonic stage

Sec23^9G

abnormal cell shape | first instar larval stage

Sec23^9G

abnormal neuroanatomy

Sec23^8-2-55

some die during embryonic stage | recessive

some die during first instar larval stage

some die during first instar larval stage (with Df(3R)ED5187)

some die during first instar larval stage (with Sec23^9G)

Sec23^CK

some die during first instar larval stage (with Sec23^CK)

Sec23^9G

Phenotype manifest in

Allele

adherens junction

Sec23^9G

basement membrane

Sec23^9G

cephalopharyngeal skeleton

cephalopharyngeal skeleton (with Df(3R)ED5187)

cephalopharyngeal skeleton (with Sec23^9G)

Sec23^CK

cephalopharyngeal skeleton (with Sec23^CK)

Sec23^9G

dendrite & dendritic arborising neuron

Sec23^8-2-55

denticle

denticle (with Df(3R)ED5187)

denticle (with Sec23^9G)

Sec23^CK

denticle (with Sec23^CK)

Sec23^9G

embryonic/first instar larval cuticle

embryonic/first instar larval cuticle (with Df(3R)ED5187)

embryonic/first instar larval cuticle (with Sec23^9G)

Sec23^CK

embryonic/first instar larval cuticle (with Sec23^CK)

Sec23^9G

embryonic/larval epidermis

Sec23^9G

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

Sec23^HMS00356

embryonic/larval tracheal dorsal trunk | embryonic stage 17

Sec23^9G

embryonic/larval tracheal section

embryonic/larval tracheal section (with Df(3R)ED5187)

embryonic/larval tracheal section (with Sec23^9G)

Sec23^CK

embryonic/larval tracheal section (with Sec23^CK)

Sec23^9G

embryonic/larval tracheal section | first instar larval stage

Sec23^9G

endoplasmic reticulum | late embryonic stage

Sec23^9G

epicuticle

Sec23^9G

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

Sec23^HMS00356

Golgi apparatus | embryonic stage 16

Sec23^9G

larval apodeme

Sec23^9G

male germ cell nucleus | increased number, with Scer\GAL4^VP16.bam

Sec23^NIG.1250R

procuticle

Sec23^9G

septate junction

Sec23^9G

spermatid, with Scer\GAL4^VP16.bam

Sec23^NIG.1250R

taenidial fold | first instar larval stage

Sec23^9G

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (6)

Hsap\SEC23A

14 of 14

Yes

Hsap\SEC23B

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (6)

Rnor\Sec23a

14 of 14

Yes

Rnor\Sec23b

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (6)

Mmus\Sec23a

14 of 14

Yes

Mmus\Sec23b

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (7)

Xtro\sec23a

11 of 13

Yes

Xtro\sec23b

10 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (6)

Drer\sec23a

13 of 14

Yes

Drer\sec23b

12 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\sec-23

14 of 14

Yes

Cele\sec-24.1

1 of 14

Cele\sec-24.2

1 of 14

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP001759

12 of 12

Yes

Agam\AgaP_AGAP005263

1 of 12

Arabidopsis thaliana (thale-cress) (10)

Atha\AT2G21630

13 of 13

Yes

Atha\AT1G05520

12 of 13

Yes

Atha\AT3G23660

12 of 13

Yes

Atha\AT4G14160

12 of 13

Yes

Atha\AT5G43670

8 of 13

Yes

Atha\AT4G01810

3 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (5)

Scer\SEC23

13 of 13

Yes

Scer\NEL1

4 of 13

Yes

Scer\SEC24

1 of 13

Scer\SFB2

1 of 13

Scer\SFB3

1 of 13

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\sec231

12 of 12

Yes

Spom\sec2302

10 of 12

Yes

Spom\sec24

1 of 12

Spom\SPBC4.03c

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Sec23. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (2)

Sec24AB

1 of 13

Sec24CD

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 3 )

Human Ortholog

Disease

Evidence

References

SEC23A; SEC23 homolog A, COPII component

model of craniolenticulosutural dysplasia

IEA

(FlyBase, 2019-)

SEC23B; SEC23 homolog B, COPII component

model of Cowden syndrome 7

IEA

(FlyBase, 2019-)

model of congenital dyserythropoietic anemia type II

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SEC23A; SEC23 homolog A, COPII component

14 of 14

610511

CRANIOLENTICULOSUTURAL DYSPLASIA; CLSD

craniolenticulosutural dysplasia

SEC23B; SEC23 homolog B, COPII component

13 of 14

610512

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Sec23 - Cpsf6

experimental knowledge based

(Guruharsha et al., 2011)

Sec23 - Gtpbp2

experimental knowledge based

(Guruharsha et al., 2011)

Sec23 - Lst8

anti tag coimmunoprecipitation, peptide massfingerprinting

(Glatter et al., 2011)

Sec23 - MED17

experimental knowledge based

(Guruharsha et al., 2011)

Sec23 - MED8

experimental knowledge based

(Guruharsha et al., 2011)

Sec23 - PAPLA1

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Kunduri et al., 2014)

Sec23 - ebi

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

cactin

suppressible

Sec23

(Miao et al., 2022)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0262125

MIST (protein-protein) - An integrated Molecular Interaction Database

40694

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Protein processing in endoplasmic reticulum

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

gene

gene_with_edited_transcript

(Rodriguez et al., 2012, Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47.5

Cytogenetic map

83B7-83B8

Sequence location

3R:5,649,065..5,652,629 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

83B7-83B8

Limits computationally determined from genome sequence between P{lacW}Atu^s1938 and P{lacW}cas^j1C2

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

86B3-86B7

(Rabouille, 2000.3.15)

83B6-83B7

(determined by in situ hybridisation)

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-47.5

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

3-48

(Tearle and Nusslein-Volhard, 1987)

3-48.4

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

19921

y¹ w^67c23; P{EPgy2}Sec23^EY06757

79400

y¹ sc^* v¹ sev²¹; P{TKO.GS00801}attP40

32365

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00356}attP2

78874

y¹ w^*; TI{CRIMIC.GT36}Sec23^CR00083/TM3, Sb¹ Ser¹

10218

w¹¹¹⁸; P{lacW}Sec23^j13C8, l(3)j13C8^j13C8/TM3, P{ftz-lacC}SC1, ry^RK Sb¹ Ser¹

3094

ru¹ hry¹ Diap1^th-1 st¹ Sec23^9G cu¹ sr¹ e^s ca¹/TM3, Sb¹ Ser¹

NIG-Fly

1250R-1

P{NIG.1250R}1

HMS00356

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00356}attP2

VDRC

v110568

P{KK100659}VIE-260B

v24552

w¹¹¹⁸; P{GD7746}v24552/TM3

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (190)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE35250

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0262125

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0262125

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

S2R+-Sec23-GFP-6

Other Stable Cell Lines

New stable cell line derived from S2-unspecified : The following stable S2 cell lines were used: Delta-WTNdeMYC, fng-GFP, Sar1[H74G]-GFP or GFP-Sec23.
(Ivan et al., 2008)

Other Comments

S2 cells treated with dsRNA directed against sec23 show a marked abnormality in actin polymerization and a total absence of bacterial binding in a phagocytosis assay.

(Stuart et al., 2007)

Mutants have reduced dendritic arbors but normal axonal growth in the class IV dendritic arborization (da) neurons.

(Ye et al., 2007)

hau mutants display only a visible head skeleton, no differentiation of cuticle.

(Jurgens et al., 1984)

Relationship to Other Genes

Source for database merge of

Source for merge of: sec23 hau

(Norum et al., 2010)

Additional comments

Source for identity of sec23 CG1250 was sequence comparison ( date:000327 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: sec23 CG1250

(FlyBase, 1996-)

Source for identity of: Sec23 sec23

(FlyBase, 2009-)

Nomenclature comments

FlyBase Curator comment: Although the "hau" symbol has precedence over the "sec23" symbol, "sec23" has been retained as the approved FlyBase symbol for this gene because of its currency in the literature.

(Norum et al., 2010)

Etymology

Named "Sec23" after the S. cerevisiae ortholog.

(FlyBase, 2009-)

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

CG1250

(Hosono et al., 2015, Christis and Munro, 2012, Glatter et al., 2011, Ni et al., 2010.12.1, Norum et al., 2010, Keleman et al., 2009.8.5, Sepp et al., 2008, Dorer et al., 2006, Dunne et al., 2002, Levis, 2002.12.19, Kondylis et al., 2001, Rabouille, 2000.5.2)

COPII

(Feng et al., 2020, Kunduri et al., 2014, Aikin et al., 2012)

Hau

(Moussian, 2013)

SEC23

(Dorer et al., 2006)

Sec23

(Dzaki and Alenius, 2024, Matsuura et al., 2024, Mitchell et al., 2023, Hodgson et al., 2022, Miao et al., 2022, Song et al., 2022, Anqueira-González et al., 2021, Evans et al., 2021, Götz et al., 2021, Pang et al., 2021, Ricolo et al., 2021, Yang et al., 2021, Gene Disruption Project members, 2018-, Chung et al., 2017, Liu et al., 2017, Ríos-Barrera et al., 2017, Transgenic RNAi Project members, 2017-, Aguilera-Gomez et al., 2016, DeBruhl et al., 2016, Copf, 2015, Kumichel et al., 2015, Ng and Jackson, 2015, DeBruhl, 2014.2.5, Gupta et al., 2014, Kunduri et al., 2014, Zacharogianni et al., 2014, Zacharogianni and Rabouille, 2013, Aikin et al., 2012, Kondylis et al., 2011, Pastor-Pareja and Xu, 2011, Zacharogianni et al., 2011, Norum et al., 2010, Vacaru et al., 2009, Beller et al., 2008, Ivan et al., 2008, Castro et al., 2007, Stuart et al., 2007, Ye et al., 2007, Bernards, 2003)

Sec23A

(Rabouille, 2000.5.2)

Sec23p

(Sone et al., 2009)

dSec23

(Kondylis et al., 2011, Sinka et al., 2008, Friggi-Grelin et al., 2006, Dunne et al., 2002, Dunne and Rabouille, 2001)

dSec23p

(Castro et al., 2007, Kondylis et al., 2007, Kondylis et al., 2005, Kondylis and Rabouille, 2003, Kondylis et al., 2001)

dar2

(Ye et al., 2007)

dsec23

(Dunne et al., 2002)

dsec23p

(Herpers and Rabouille, 2004)

hau

(Kumichel et al., 2015, Norum et al., 2010)

Name Synonyms

Haunted

(Moussian, 2013, Norum et al., 2010)

Sec23 ortholog

Secretory 23

coat protein II

(Kunduri et al., 2014)

dendritic arbor reduction 2

(Ye et al., 2007)

haunted

(Wieschaus and Nüsslein-Volhard, 2016, Kumichel et al., 2015, Norum et al., 2010)

sec23

(Sepp et al., 2008)

Secondary FlyBase IDs

FBgn0001178
FBgn0037357
FBgn0011441

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 74 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40694

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0B4K5Z8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0B4K5Z8

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0262125

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40694/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0262125

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0262125

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1250

MARRVEL_MODEL - MARRVEL (model organism gene)

40694

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0262125

DroID - A comprehensive database of gene and protein interactions.

FBgn0262125

DRSC - Results frm RNAi screens

FBgn0262125

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Sec23_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0037357

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0262125

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1250

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009191

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Protein processing in endoplasmic reticulum

MIST (protein-protein) - An integrated Molecular Interaction Database

40694

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (143)

The following external sites may use different assemblies or annotations than FlyBase.

1.02

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