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FB2026_01
,
released March 12, 2026
P450, cytochrome P450, P-450-B
Please see the JBrowse view of Dmel\Cyp6a2 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.48
Gene model reviewed during 5.43
There is only one protein coding transcript and one polypeptide associated with this gene
507 (aa); 59 (kD predicted)
56 (kD)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cyp6a2 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Cyp6a2 protein is 10-20 times more prevalent in insecticide resistant strains than in susceptible strains.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Cyp6a2 in JBrowsePlease Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Expression of Cyp6a2 is inducible by phenobarbital but constitutive in DDT resistant strains.
Identified molecularly using degenerate PCR primers designed from conserved regions of known insect P450 genes.
Molecular data about the genomic structure of Cyp6a2, its transcription during different developmental stages and its tissue-specific expression in larvae is studied.
Presence or absence of a long terminal repeat of 17.6 is uncorrelated with resistance or susceptibility to DDT in 31 strains of D.melanogaster and D.simulans from around the world.
The adenylate cyclase inactivator forskolin and its pharmacologically inactive derivative 1,9-dideoxyforskolin inhibit, in a dose-dependent fashion, the ecdysone 20-monooxygenase activity associated with wandering stage larvae.
The level of cytochrome P450-B1 monooxygenase is much higher in strains resistant to DDT. In a susceptible strain a solitary 17.6 element LTR is present in the 3' untranslated end of the gene; this is absent from a resistant strain.
Monoclonal antibody studies demonstrate that Cyt-P450-B1 expression is regulated by resistance genes on the second and third chromosomes, and is correlated with the resistance to phenylurea, DDT and malathion.
Dmnd gene expression is regulated by loci on the third chromosome.
Cyp6a2 gene expression is regulated by loci on the third chromosome, and to some extent by loci on the first and fourth chromosomes.
Dimethylnitrosamine demethylase (DMND) is a cytochrome-P450-dependent mixed function oxigenase (Waters et al., 1982). DMND has been characterised from microsomes of Hikone-R strain by Waters, Nix and Epler (1982) and correlated with dimethylnitrosamine-dependent mutagenesis (Waters, Nix and Epler, 1983). Activity very low, or absent, in insecticide susceptible strains. Activity correlates with a 55.8kD cytochrome P450-B band on electrophoresis (Waters and Nix, 1988). Loci on chromosome 3 are necessary for maximal expression of DMND (Waters and Nix, 1988).
Cyp6a2 expression varies considerably between different strains. For instance, higher RNA levels are found in the 91R strain (insecticide resistant) when compared to the 91C strain (insecticide sensitive) (FBrf0057534). Monoclonal antibodies to cytochrome CYP6A2 raised by Sundseth, Kennel and Waters (1989) and these reveal protein even in insecticide-susceptible strains in which activity cannot be seen otherwise.
Source for merge of: Cyp6a2 Dmnd
FlyBase curator comment: Previous papers from Waters et al. associated dimethylnitrosamine demethylase activity (for which FlyBase created the 'Dmnd' gene) with the P450-B component, which is identified here as Cyp6a2.
