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Citation
Hodgson, J.J., Buchon, N., Blissard, G.W. (2019). Identification of insect genes involved in baculovirus AcMNPV entry into insect cells.  Virology 527(): 1--11.
FlyBase ID
FBrf0241100
Publication Type
Research paper
Abstract

The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is a model enveloped DNA virus that infects and replicates in lepidopteran insect cells, and can efficiently enter a wide variety of non-host cells. Budded virions of AcMNPV enter cells by endocytosis and traffic to the nucleus where the virus initiates gene expression and genome replication. While trafficking of nucleocapsids by actin propulsion has been studied in detail, other important components of trafficking during entry remain poorly understood. We used a recombinant AcMNPV virus expressing an EGFP reporter in combination with an RNAi screen in Drosophila DL1 cells, to identify host proteins involved in AcMNPV entry. The RNAi screen targeted 86 genes involved in vesicular trafficking, including genes coding for VPS and ESCRT proteins, Rab GTPases, Exocyst proteins, and Clathrin adaptor proteins. We identified 24 genes required for efficient virus entry and reporter expression, and 4 genes that appear to restrict virus entry.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Virology
    Title
    Virology
    Publication Year
    1955-
    ISBN/ISSN
    0042-6822 1096-0341
    Data From Reference
    Genes (28)
    Cell Lines (1)