GFP(N146I) is a green fluorescent protein. It is an artificial derivative of the naturally occurring fluorescent protein encoded by the Aequorea victoria GFP gene (UniProtKB:P42212), containing the mutation N146I that has 10 times less affinity for the GFP nanobody (VHH(GFP4)) compared to wild type GFP (PMID:20945358, PMID: 33139893).