FlyBase Allele Report: Dmel\N[int.U.UAS]

Expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^tj-NP1624 leads to ovaries with a large increase in the number of cap cells, as compared to controls, and some germaria with a cluster of undifferentiated germ cells that resemble germline stem cells, while other germaria are devoid of germ cells altogether.

(Panchal et al., 2017)

Expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^elav-C155 in the larval optic lobe (in combination with a Gal80[ts] transgene to restrict expression to the larval stage) leads to ectopic neuroblasts among medulla neurons.

Larval optic lobe post-mitotic neuron clones expressing N^{int.U.Scer\UAS} under the control of Scer\GAL4^Act.PU include ectopic neuroblasts.

(Xu et al., 2017)

Somatic clones expressing N^{int.U.Scer\UAS} under the control of Scer\GAL4^Act.PU in wandering third instar wing discs present a significant increase in relative clonal area as compared to controls.

The induction of somatic clones expressing N^{int.U.Scer\UAS} under the control of Scer\GAL4^GMR.PU leads to a significant decrease in the eclosion rate, as compared to controls.

(Casas-Tintó et al., 2016)

Constitutive expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^GMR.PU does not lead to any obvious cell migration defects in the pupal eye tissue, as compared to controls.

(Padash Barmchi et al., 2016)

Expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^Antp-21 in third instar larvae results in a significant increase in crystal cell number in lymph gland anterior lobes, and these develop in abnormal clusters, in contrast to controls.

Expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} in third instar lymph gland clones induces crystal cell fate both in the primary lobe, but occasionally also in the posterior lobe, where crystal cells are not normally found.

(Small et al., 2014)

Clones expressing N^{int.U.Scer\UAS} under the control of Scer\GAL4^Act5C.PU show an increase in the number of neuroblasts per clone compared to wild type.

(Lee et al., 2013)

The gl-positive corpora cardiaca precursor cells are missing in stage 12 embryos expressing N^{int.U.Scer\UAS} under the control of either Scer\GAL4^twi.2PE or Scer\GAL4^Mef2.PR.

The number of gl-positive corpora cardiaca precursor cells present at stage 12 is not altered by expression of N^{int.U.Scer\UAS} under the control of either Scer\GAL4^gl.5.2 or Scer\GAL4^gt.1.CRM.

(Park et al., 2011)

Transient expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B for seven days in young flies shortens lifespan significantly compared to controls. Longevity is not significantly affected when these flies are kept at permissive temperature throughout life.

(Biteau et al., 2010)

Expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^dpp.3KK eliminates the wing veins.

(Molnar et al., 2006)

Expression of N^{int.U.Scer\UAS} in the eye disc under the control of Scer\GAL4^dpp.blk1 results in larger third instar larval eye discs compared to wild-type. Proper R8 neuron specification is observed. Neuronal clusters are highly disorganised or absent in the posterior region of the disc.

Expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in disruption of cluster assembly and neuronal differentiation in late third instar larval eye discs. Nearly normal selection of R8 neurons occurs, but aberrant formation of more posterior clusters, completely lacking R8 cells and with reduced numbers of photoreceptors.

(Zhang et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

N^int.U.UAS, Scer\GAL4^Act.PU has abnormal size | wandering third instar larval stage | somatic clone phenotype, enhanceable by wupA^f06492, Scer\GAL4^Act.PU

(Casas-Tintó et al., 2016)

Suppressed by

Statement

Reference

N^int.U.UAS, Scer\GAL4^GMR.PU has partially lethal - majority die phenotype, suppressible by wupA^JF02172, Scer\GAL4^GMR.PU

(Casas-Tintó et al., 2016)

N^int.U.UAS, Scer\GAL4^repo.PU has abnormal mitotic cell cycle phenotype, suppressible by Ser^sec.UAS, Scer\GAL4^repo.PU

(Pérez-Gómez et al., 2013)

NOT Enhancer of

Statement

Reference

N^int.U.UAS, Scer\GAL4^Act.PU is a non-enhancer of abnormal size | wandering third instar larval stage | somatic clone phenotype of Scer\GAL4^Act.PU, wupA^f06492

(Casas-Tintó et al., 2016)

Suppressor of

Statement

Reference

N^int.U.UAS, Scer\GAL4^esg.PU is a suppressor of visible | adult stage phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

N^int.U.UAS, Scer\GAL4^esg.PU is a suppressor of abnormal size | adult stage phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

NOT Suppressor of

Statement

Reference

N^int.U.UAS, Scer\GAL4^bbg-C96 is a non-suppressor of visible phenotype of Scer\GAL4^bbg-C96, Taf9^RNAi.UAS.cUa

(Xie et al., 2014)

Other

Statement

Reference

Dronc^KO/Dronc^KO.-FRT.QF, N^int.U.UAS, Scer\GAL4^esg.PU has decreased cell number | somatic clone | adult stage phenotype

(Arthurton et al., 2020)

N^int.U.UAS, Scer\GAL4^NP1624, tj^NIG.10034R has abnormal size | adult stage phenotype

(Panchal et al., 2017)

N^int.U.UAS, Scer\GAL4^NP1624, tj^NIG.10034R has decreased cell number | adult stage phenotype

(Panchal et al., 2017)

N^int.U.UAS, Scer\GAL4^Act.PU, wupA^f06492 has visible | somatic clone | adult stage phenotype

(Casas-Tintó et al., 2016)

N^int.U.UAS, Scer\GAL4^Act.PU, wupA^f06492 has partially lethal - majority die | somatic clone phenotype

(Casas-Tintó et al., 2016)

N^int.U.UAS, Scer\GAL4^Act.PU, wupA^f06492 has neoplasia | somatic clone | adult stage phenotype

(Casas-Tintó et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, N^int.U.UAS, Scer\GAL4^GMR.PU has abnormal cell migration | pupal stage phenotype

(Padash Barmchi et al., 2016)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, N^int.U.UAS, Scer\GAL4^GMR.PU has lethal - all die before end of P-stage phenotype

(Padash Barmchi et al., 2016)

N^int.U.UAS, Nct^ST3/Nct^ST1, Scer\GAL4^dpp.blk1 has increased cell number phenotype

(Zhang et al., 2005)

Phenotype Manifest In

Enhanced by

Statement

Reference

N^int.U.UAS, Scer\GAL4^Act.PU has wing disc | wandering third instar larval stage | somatic clone phenotype, enhanceable by wupA^f06492, Scer\GAL4^Act.PU

(Casas-Tintó et al., 2016)

Suppressed by

Statement

Reference

N^int.U.UAS, Scer\GAL4^Act5C.PU has neuroblast | increased number | somatic clone phenotype, suppressible by Drp1²

(Lee et al., 2013)

N^int.U.UAS, Scer\GAL4^repo.PU has glial cell phenotype, suppressible by Ser^sec.UAS, Scer\GAL4^repo.PU

(Pérez-Gómez et al., 2013)

N^int.U.UAS, Scer\GAL4^dpp.blk1 has ommatidial precursor cluster | wandering third instar larval stage phenotype, suppressible by Nct^ST3/Nct^ST1

(Zhang et al., 2005)

NOT suppressed by

Statement

Reference

N^int.U.UAS, Scer\GAL4^twi.PB has dorsal acute muscle cell | precursor phenotype, non-suppressible by pnt^P2.VP16.UAS, Scer\GAL4^twi.PB

(Carmena et al., 2002)

N^int.U.UAS, Scer\GAL4^twi.PB has dorsal oblique muscle cell | precursor phenotype, non-suppressible by pnt^P2.VP16.UAS, Scer\GAL4^twi.PB

(Carmena et al., 2002)

N^int.U.UAS, Scer\GAL4^twi.PB has pericardial cell | precursor phenotype, non-suppressible by pnt^P2.VP16.UAS, Scer\GAL4^twi.PB

(Carmena et al., 2002)

NOT Enhancer of

Statement

Reference

N^int.U.UAS, Scer\GAL4^Act.PU is a non-enhancer of wing disc | wandering third instar larval stage | somatic clone phenotype of Scer\GAL4^Act.PU, wupA^f06492

(Casas-Tintó et al., 2016)

Suppressor of

Statement

Reference

N^int.U.UAS, Scer\GAL4^esg.PU is a suppressor of adult abdominal fat mass phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

N^int.U.UAS, Scer\GAL4^esg.PU is a suppressor of ovary | adult stage phenotype of Scer\GAL4^esg.PU, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Pranoto et al., 2023)

N^int.U.UAS/Scer\GAL4^dpp.blk1 is a suppressor of eye photoreceptor cell | wandering third instar larval stage phenotype of Nct^ST3/Nct^ST1

(Zhang et al., 2005)

N^int.U.UAS/Scer\GAL4^dpp.blk1 is a suppressor of eye disc | wandering third instar larval stage phenotype of Nct^ST3/Nct^ST1

(Zhang et al., 2005)

NOT Suppressor of

Statement

Reference

N^int.U.UAS, Scer\GAL4^bbg-C96 is a non-suppressor of wing margin phenotype of Scer\GAL4^bbg-C96, Taf9^RNAi.UAS.cUa

(Xie et al., 2014)

Other

Statement

Reference

N^int.U.UAS, Scer\GAL4^NP1624, tj^NIG.10034R has germline cell | adult stage phenotype

(Panchal et al., 2017)

N^int.U.UAS, Scer\GAL4^NP1624, tj^NIG.10034R has ovarian sheath | adult stage phenotype

(Panchal et al., 2017)

N^int.U.UAS, Scer\GAL4^NP1624, tj^NIG.10034R has ovary | adult stage phenotype

(Panchal et al., 2017)

N^int.U.UAS, Scer\GAL4^NP1624, tj^NIG.10034R has terminal filament | adult stage phenotype

(Panchal et al., 2017)

HPV18\E6^UAS.Tag:MYC, Hsap\UBE3A^UAS.cRa, N^int.U.UAS, Scer\GAL4^ap.PU has imaginal disc epithelial cell | pupal stage phenotype

(Padash Barmchi et al., 2016)

N^int.U.UAS, Nct^ST3/Nct^ST1, Scer\GAL4^dpp.blk1 has eye disc | wandering third instar larval stage phenotype

(Zhang et al., 2005)

N^int.U.UAS, Nct^ST3/Nct^ST1, Scer\GAL4^dpp.blk1 has photoreceptor cell R8 | increased number | wandering third instar larval stage phenotype

(Zhang et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Adult posterior midgut clones specifically generated in esg-positive progenitors (ISCs and EBs), and which both express N^int.U.UAS under the control of Scer\GAL4^esg.PU and are Dronc^KO.-FRT.QF/Dronc^KO transheterozygous show decreased proportion of progenitors.

(Arthurton et al., 2020)

Expression of both N^{int.U.Scer\UAS} and tj^NIG.10034R under the control of Scer\GAL4^tj-NP1624 leads to severe ovary defects. Most ovaries are extremely small, not connected to an oviduct and often attached to the gut, and consist largely of terminal filaments and a few germline cells embedded in fat body. The epithelial sheaths are missing and terminal filaments are located side-by-side. In the most severe cases, these ovaries consist only of terminal filaments. Terminal filaments in these flies are on average longer than those in flies expressing only N^{int.U.Scer\UAS}, but slightly shorter than those in flies expressing only tj^NIG.10034R.

(Panchal et al., 2017)

Somatic clones co-expressing wupA^f06492 and N^{int.U.Scer\UAS} under the control of Scer\GAL4^Act.PU in wandering third instar larval wing discs present a significant increase in the relative clonal area, as compared to clones only expressing N^{int.U.Scer\UAS} but not to clones only expressing wupA^f06492; animals bearing clones co-expressing wupA^f06492 and N^{int.U.Scer\UAS} occasionally reach adulthood and present visible outgrowths.

The co-expression of wupA^JF02172 suppresses the decreased eclosion rate of individuals bearing somatic clones expressing N^{int.U.Scer\UAS} under the control of Scer\GAL4^GMR.PU.

(Casas-Tintó et al., 2016)

Drp1² suppresses the increase in the number of neuroblasts per clone seen in clones expressing N^{int.U.Scer\UAS} under the control of Scer\GAL4^Act5C.PU.

(Lee et al., 2013)

Expression of N^{int.U.Scer\UAS} under the control of Scer\GAL4^dpp.blk1 in a nct^ST1/nct^ST3 mutant background results in larger third instar eye discs compared to wild-type. The missing neuronal clusters observed in N^{int.U.Scer\UAS}; Scer\GAL4^dpp.blk1 eye discs are restored in a nct^ST1/nct^ST3 mutant background. However, R8 cells are not as regularly arrayed as in wild-type or N^{int.U.Scer\UAS}; Scer\GAL4^dpp.blk1 eye discs. An increase R8 cell number is also observed, including more than one R8 cell per cluster.

(Zhang et al., 2005)

Xenogenetic Interactions

Statement

Reference

The co-expression of N^{int.U.Scer\UAS} and Hsap\UBE3A^Scer\UAS.cRa with HPV18\E6^{Scer\UAS.T:Hsap\MYC}, but not with HPV18\E6^{V158A.Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^GMR.PU, leads to the migration of a considerable proportion of the expressing eye cells into distant pupal tissues (e.g. abdomen), as compared to controls.

(Padash Barmchi et al., 2016)

Complementation and Rescue Data

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Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

N^act

(Lee et al., 2013)

N^{int.U.Scer\UAS}

N^int.U.UAS

N^intra

(Canales Coutiño et al., 2020)

NΔECD

(Feng et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (85)

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