FlyBase Allele Report: Dmel\spi[s.UAS]

General Information

Symbol

Dmel\spi^s.UAS

Species

D. melanogaster

Name

secreted

FlyBase ID

FBal0046516

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

Carried in Construct

P{UAS-spi.s}

Also Known As

UAS-sSpi, UAS-sSpitz, UAS-sSpi4a, Sspi, UASp-sSpi

Key Links

Transgenic product class

stop_gained

(Schweitzer et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

stop_gained

(Schweitzer et al., 1995)

Mutagen

in vitro construct

(Buff et al., 1998, Hazelett et al., 1998, Kerber et al., 1998, Gabay et al., 1997, Szuts et al., 1997, Schweitzer et al., 1995)

Progenitor genotype

spi^UAS.cSa

(Schweitzer et al., 1995)

Carried in construct

P{UAS-spi.s}

Cytology

Description

UASt regulatory sequences drive expression of a secreted form of spi (a stop codon has been introduced at K129, the putative cleavage site of the precursor protein).

(Schweitzer et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

spi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intestinal cancer

CEA

(Chen et al., 2016)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle, with Scer\GAL4^GMR.PF

(Yang and Baker, 2003)

abnormal neuroanatomy | embryonic stage 13, with Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

abnormal neuroanatomy | embryonic stage 16, with Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

decreased cell number, with Scer\GAL4^{VP16.nanos.UTR}

(Gilboa and Lehmann, 2006)

increased cell number | embryonic stage 12

(Maybeck and Röper, 2009)

increased cell number | embryonic stage 13

(Maybeck and Röper, 2009)

increased cell number | third instar larval stage, with Scer\GAL4^NP7397

(Jiang and Edgar, 2009)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP5130

(Buchon et al., 2010)

increased occurrence of cell division | adult stage, with Scer\GAL4^NP7397

(Perea et al., 2017)

lethal, with Scer\GAL4^cb41

(McDonald et al., 2006)

lethal, with Scer\GAL4^h-H10

(Lesokhin et al., 1999)

lethal | female | pupal stage, with Scer\GAL4^71B

(Wessells et al., 1999)

lethal | male | pharate adult stage, with Scer\GAL4^71B

(Wessells et al., 1999)

lethal - all die before end of larval stage, with Scer\GAL4^hs.PB

(Freeman, 1996)

lethal - all die before end of pupal stage, with Scer\GAL4^GMR.PF

(Freeman, 1996)

neoplasia | adult stage, with Scer\GAL4^Su(H).GBE

(Chen et al., 2016)

some die during larval stage, with Scer\GAL4^hs.PB

(Freeman, 1996)

some die during pupal stage, with Scer\GAL4^GMR.PF

(Freeman, 1996)

visible, with Scer\GAL4^bs-1348

(Schnepp et al., 1998)

visible, with Scer\GAL4^GMR.PF

(Schnepp et al., 1998)

visible, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

visible | female, with Scer\GAL4^71B

(Wessells et al., 1999)

visible | homeotic, with Scer\GAL4^ey.PB

(Kumar and Moses, 2001)

Phenotype Manifest In

abdominal lateral pentascolopidial chordotonal organ lch5 | increased number, with Scer\GAL4^en-e16E

(Elstob et al., 2001)

adult midgut, with Scer\GAL4^Su(H).GBE

(Chen et al., 2016)

adult midgut progenitor cell | third instar larval stage, with Scer\GAL4^NP7397

(Jiang and Edgar, 2009)

adult subretinal glial cell, with Scer\GAL4^GMR.PF

(Firth and Baker, 2007)

antenna | ectopic, with Scer\GAL4^ey.PB

(Kumar and Moses, 2001)

border follicle cell, with Scer\GAL4^slbo.2.6

(McDonald et al., 2006, Jekely et al., 2005)

border follicle cell | somatic clone, with Scer\GAL4^cb41

(McDonald et al., 2006)

bract & leg | ectopic, with Scer\GAL4^sca-537.4

(Held, 2002)

bract | ectopic, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

denticle, with Scer\GAL4^69B

(Payre et al., 1999)

denticle, with Scer\GAL4^e22c

(Payre et al., 1999)

denticle | ectopic, with Scer\GAL4^arm.PS

(Urban et al., 2002)

denticle | ectopic, with Scer\GAL4^en-e16E

(O'Keefe et al., 1997)

denticle row 5, with Scer\GAL4^arm.PS

(Szuts et al., 1997)

denticle row 6, with Scer\GAL4^arm.PS

(Szuts et al., 1997)

dorsal appendage, with Scer\GAL4^55B

(Sapir et al., 1998)

embryonic/first instar larval cuticle, with Scer\GAL4^en-e16E

(O'Keefe et al., 1997)

embryonic/first instar larval cuticle & denticle, with Scer\GAL4^en-e16E

(O'Keefe et al., 1997)

embryonic/larval cuticle, with Scer\GAL4^arm.PS

(Szuts et al., 1997)

embryonic/larval oenocyte | ectopic, with Scer\GAL4^ato.3.6

(Brodu et al., 2002)

embryonic/larval oenocyte | ectopic, with Scer\GAL4^en-e16E

(Brodu et al., 2002)

embryonic/larval oenocyte | increased number, with Scer\GAL4^en-e16E

(Elstob et al., 2001)

embryonic/larval optic lobe, with Scer\GAL4^unspecified

(Daniel et al., 1999)

embryonic/larval salivary gland, with Scer\GAL4^fkh.PH

(Maybeck and Röper, 2009)

embryonic/larval wrapping glial cell | third instar larval stage, with Scer\GAL4^repo.PL

(Matzat et al., 2015)

embryonic epidermis, with Scer\GAL4^69B

(Payre et al., 1999)

embryonic epidermis, with Scer\GAL4^e22c

(Payre et al., 1999)

embryonic epidermis, with Scer\GAL4^Kr.PM

(Schweitzer et al., 1995)

embryonic mesothoracic segment, with Scer\GAL4^ato.3.6

(Brodu et al., 2002)

embryonic mesothoracic segment, with Scer\GAL4^en-e16E

(Brodu et al., 2002)

embryonic metathoracic segment, with Scer\GAL4^ato.3.6

(Brodu et al., 2002)

embryonic metathoracic segment, with Scer\GAL4^en-e16E

(Brodu et al., 2002)

embryonic prothoracic segment, with Scer\GAL4^ato.3.6

(Brodu et al., 2002)

embryonic prothoracic segment, with Scer\GAL4^en-e16E

(Brodu et al., 2002)

enterocyte | increased number, with Scer\GAL4^NP0001

(Jiang et al., 2011)

eye, with Scer\GAL4^ey.PB

(Kumar and Moses, 2001)

eye, with Scer\GAL4^GMR.PF

(Schnepp et al., 1998)

eye disc | somatic clone, with Scer\GAL4^Act5C.PU

(Steinhauer et al., 2013)

filzkorper, with Scer\GAL4^ems.HRE

(Merabet et al., 2005)

filzkorper, with Scer\GAL4^salm-459.2

(Merabet et al., 2005)

germline stem cell, with Scer\GAL4^{VP16.nanos.UTR}

(Gilboa and Lehmann, 2006)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP7397

(Perea et al., 2017)

lamellocyte | larval stage, with Scer\GAL4^Ugt36A1.PK

(Sinenko et al., 2011)

lamina & neuron, with Scer\GAL4^hs.PB

(Huang et al., 1998)

larval DA1 motor neuron, with Scer\GAL4^sim.PS

(Menne et al., 1997)

larval dorsal hair, with Scer\GAL4^55B

(Sapir et al., 1998)

larval thoracic perisympathetic organ, with Scer\GAL4^sim.PS

(Luer et al., 1997)

lch5 ligament attachment cell | increased number, with Scer\GAL4^repo

(Inbal et al., 2004)

leg disc, with Scer\GAL4^Dll-md23

(Kubota et al., 2000)

mesothoracic tarsal segment 1 & bract | ectopic, with Scer\GAL4^sca-537.4

(Held, 2002)

mesothoracic tibial apical bristle, with Scer\GAL4^Dll-md23

(Held, 2002)

mesothoracic tibial apical bristle, with Scer\GAL4^sca-537.4

(Held, 2002)

mesothoracic tibial bract | ectopic, with Scer\GAL4^sca-537.4

(Held, 2002)

midgut, with Scer\GAL4^NP5130, Scer\GAL80^ts.αTub84B

(Buchon et al., 2010)

midgut interstitial cell | increased number, with Scer\GAL4^NP7397

(Jiang et al., 2011)

midline glial cell, with Scer\GAL4^hs.PB

(Bergmann et al., 2002)

midline glial cell | embryonic stage 13 | increased number, with Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

midline glial cell | embryonic stage 16 | increased number, with Scer\GAL4^sim.P3.7

(Zhang et al., 2011)

muscle founder cell | embryonic stage, with Scer\GAL4^twi.PG

(Buff et al., 1998)

neuron | ectopic, with Scer\GAL4^hs.PB

(Huang et al., 1998)

ommatidium, with Scer\GAL4^GMR.PF

(Lesokhin et al., 1999)

ommatidium, with Scer\GAL4^hs.PB

(Freeman, 1996)

pCC neuron, with Scer\GAL4^sim.PS

(Menne et al., 1997)

photoreceptor | ectopic | somatic clone, with Scer\GAL4^Act5C.PU

(Steinhauer et al., 2013)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Freeman, 1996)

photoreceptor neuron, with Scer\GAL4^hs.PB

(Freeman, 1996)

presumptive embryonic/larval peripheral nervous system, with Scer\GAL4^en-e16E

(Elstob et al., 2001)

prothoracic tibial apical bristle, with Scer\GAL4^Dll-md23

(Held, 2002)

prothoracic tibial apical bristle, with Scer\GAL4^sca-537.4

(Held, 2002)

retina, with Scer\GAL4^GMR.PF

(Lesokhin et al., 1999)

stigmatophore, with Scer\GAL4^ems.HRE

(Merabet et al., 2005)

stigmatophore, with Scer\GAL4^salm-459.2

(Merabet et al., 2005)

stomatogastric nervous system anlage, with Scer\GAL4^Act.PU

(Gonzalez-Gaitan and Jackle, 2000)

taste bristle & leg | ectopic, with Scer\GAL4^Dll-md23

(Held, 2002)

taste bristle & leg | ectopic, with Scer\GAL4^sca-537.4

(Held, 2002)

trichome, with Scer\GAL4^hs.PB

(del Alamo et al., 2002)

ventral denticle belt, with Scer\GAL4^55B

(Sapir et al., 1998)

ventral denticle belt, with Scer\GAL4^arm.PS

(Szuts et al., 1997)

wing, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

wing | female, with Scer\GAL4^71B

(Wessells et al., 1999)

wing disc, with Scer\GAL4^71B

(Wessells et al., 1999)

wing disc, with Scer\GAL4^Dll-md23

(Kubota et al., 2000)

wing disc, with Scer\GAL4^dpp.blk1

(Nagaraj et al., 1999)

wing hair | female, with Scer\GAL4^71B

(Wessells et al., 1999)

wing vein, with Scer\GAL4^bs-1348

(Schnepp et al., 1998)

wing vein | ectopic, with Scer\GAL4^kni.L2

(Lunde et al., 2003)

wing vein | ectopic | female, with Scer\GAL4^71B

(Wessells et al., 1999)

Detailed Description

Statement

Reference

Adult flies expressing spi^s.Scer\UAS driven by Scer\GAL4^esg-NP7397 combined with tub-Gal80[ts] (for temporal control) display significant increase in midgut stem cell proliferation (assessed by number of pH3-positive cells).

(Perea et al., 2017)

Expression of spi^s.Scer\UAS under the control of Scer\GAL4^Su(H).GBE results in enteroblast-like tumors in the intestine.

(Chen et al., 2016)

Expression of spi^s.UAS under the control of Scer\GAL4^repo.PL result in significantly increased axon wrapping index (proportion of axon clusters wrapped by wrapping glia), whereas expression under Scer\GAL4^elav.PU does not affect the axon wrapping by the wrapping glia in third instar larvae.

(Matzat et al., 2015)

Somatic clones in eye imaginal discs that express spi^s.Scer\UAS under the control of Scer\GAL4^Act5C.PU recruit ectopic photoreceptors.

(Steinhauer et al., 2013)

Expression of spi^s.Scer\UAS in the midgut, under the control of Scer\GAL4^esg-NP7397 results in the generation of many new midgut cells, including enterocyte-like cells.

Expression of spi^s.Scer\UAS in mature enterocytes, under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B results in the generation of many new midgut cells, including enterocyte-like cells. However, expression in enterocyte cells, under the control of Scer\GAL4^{Myo31DF-NP0001} and Scer\GAL80^ts.αTub84B fails to affect midgut mitosis.

(Jiang et al., 2011)

Expression of spi^s.Scer\UAS in the posterior signalling center (PSC) under the control of Scer\GAL4^Dot.PK leads to an increase in the number of circulating lamellocytes

(Sinenko et al., 2011)

Stage 13 embryos expressing spi^s.Scer\UAS under the control of Scer\GAL4^sim.P3.7 have approximately 8 anterior midline glia (AMG) compared to six in wild type. By stage 16 the number of AMG has reduced to 5, compared to 3 in wild type.

(Zhang et al., 2011)

Expression of spi^s.Scer\UAS in precursor cells under the control of Scer\GAL4^esg-NP5130 and Scer\GAL80^ts.αTub84B induces high level intestinal stem cell (ISC) proliferation.

(Buchon et al., 2010)

Induction of spi^s.Scer\UAS by Scer\GAL4^esg-NP7397 results in extensive overproliferation of adult midgut progenitor cells, while limiting their dispersal, thus reducing the number of AMP clusters in wandering third instar larval midguts.

(Jiang and Edgar, 2009)

Over-expression using Scer\GAL4^fkh.PH leads to ectopic cells within the salivary glands and glands positioned too anteriorly without a discernible duct. Whereas control salivary gland placode cells at stages 12-13 do not undergo division, Scer\GAL4^fkh.PH spi^s.Scer\UAS placode cells are actively dividing.

(Maybeck and Röper, 2009)

Animals expressing spi^s.Scer\UAS under the control of Scer\GAL4^GMR.PF show defects in migration and localisation of glia in the eye. Basal glia in the mutant retinas migrate anterior to the morphogenetic furrow past differentiating photoreceptors. In addition, glia are also found apical to the photoreceptors and along the Bolwig nerve. These animals have more plasmatocytes on the developing retina than wild type.

(Firth and Baker, 2007)

Primordial germ cell numbers are significantly reduced when spi^s.Scer\UAS is expressed in the primordial germ cells, under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Gilboa and Lehmann, 2006)

Border cells are found off their normal migration pathway on the side of the egg chamber when spi^s.Scer\UAS is expressed under the control of Scer\GAL4^cb41 in follicle cell clones. Due to the lethality caused by expression of spi^s.Scer\UAS under the control of Scer\GAL4^cb41, this phenotype was observed in adult flies for whom expression of spi^s.Scer\UAS was initially inhibited by Scer\GAL80^ts.αTub84B and this inhibition was relieved by a temperature shift after the lethal stage.

Expression of one copy of spi^s.Scer\UAS in the border cell cluster, driven by Scer\GAL4^slbo.2.6, results in the inhibition of border cell migration in around 80% of egg chambers.

(McDonald et al., 2006)

In spi^s.Scer\UAS Scer\GAL4^slbo.2.6 egg chambers, 70% of border follicle clusters fail to migrate.

(Jekely et al., 2005)

Expression of spi^s.Scer\UAS in embryos after posterior spiracle specification, using Scer\GAL4^ems.HRE, results in stigmatophores that are reduced in size and filzkorpers that do not elongate properly. Expression of this transgene with Scer\GAL4^salm-459.2 causes stronger defects in stigmatophore size and filzkorper elongation.

(Merabet et al., 2005)

Stage 16 spi^s.Scer\UAS; Scer\GAL4^repo embryos have increased numbers of lch5 ligament attachment cells. When Scer\GAL4^69B is used instead as a driver then ectopic cells are formed near to endogenous lch5 ligament attachment cells, but these don't express all ligament attachment cell markers.

(Inbal et al., 2004)

Expression of spi^s.Scer\UAS under the control of Scer\GAL4^kni.L2 results in ectopic veins forming 2-3 cell diameters away from the L2 wing vein.

(Lunde et al., 2003)

The second mitotic wave is completely inhibited in spi^s.Scer\UAS; Scer\GAL4^GMR.PF 3rd instar eye discs.

(Yang and Baker, 2003)

Stage 17 spi^s.Scer\UAS; Scer\GAL4^hs.PB embryos have increased numbers of midline glial cells compared to wild-type.

(Bergmann et al., 2002)

Expression of spi^s.Scer\UAS under the control of Scer\GAL4^en-e16E or Scer\GAL4^ato.3.6 results in the formation of ectopic oenocytes in the T1-T3 segments (they are normally only found in segments A1-A7).

(Brodu et al., 2002)

When spi^Scer\UAS.cSa is driven by Scer\GAL4^sca-537.4 the number of bristles with extra bracts averages about 1.5 per tibia, 7 per basitarsus, and 25 per leg overall. Most of the affected bristles have two adjacent bracts of normal size, while a few (one or two bristles per leg) have three adjacent bristles in a proximal arc. Also in 3 and 1 in 10 legs (when driven by Scer\GAL4^Dll-md23 and Scer\GAL4^sca-537.4 respectively) a transformation of the huge 'apical' bristle into a chemosensory bristle of ordinary size is seen.

(Held, 2002)

spi^s.Scer\UAS (driven by Scer\GAL4^unspecified) has no effect on Malpighian tubule cell number.

(Sudarsan et al., 2002)

Extra denticles are produced in spi^s.Scer\UAS; Scer\GAL4^arm.PS embryos.

(Urban et al., 2002)

Clones in the prospective notum or wing hinge in the wing disc expressing spi^s.Scer\UAS under the control of Scer\GAL4^αTub84B.PC have wiggly borders.

(Zecca and Struhl, 2002)

When spi^s.Scer\UAS is driven by Scer\GAL4^hs.PB and heatshocked all trichomes are transformed into bracts.

(del Alamo et al., 2002)

The peripheral nervous system is highly disorganised in embryos expressing spi^s.Scer\UAS under the control of Scer\GAL4^en-e16E. Excess lateral chordotonal organs are formed with the number of lateral chordotonal organs in the cluster frequently being 6 or 7 (instead of the wild type 5). The oenocyte precursor whorl is enlarged and by stage 16 oenocyte clusters containing 21-39 cells are seen.

(Elstob et al., 2001)

Expression of spi^s.Scer\UAS under the control of Scer\GAL4^ey.PB can result in homeotic transformation of the eye to antenna.

(Kumar and Moses, 2001)

Expression of spi^s.Scer\UAS under the control of Scer\GAL4^Act.PU at 25[o]C results in a variable number of supernumerary infoldings within the stomatogastric nervous system anlagen in stage 11 embryos.

(Gonzalez-Gaitan and Jackle, 2000)

Expression of spi^s.Scer\UAS under the control of Scer\GAL4^Dll-md23 completely eliminates wing disc formation in embryos and causes a malformation of the leg disc (the leg disc has an elongated shape).

(Kubota et al., 2000)

Embryos overexpressing spi^s.Scer\UAS under the control of Scer\GAL4^unspecified show a 'cyclops' phenotype in which the optic lobes are enlarged and show dorsal fusion.

(Daniel et al., 1999)

When expression is driven by Scer\GAL4^GMR.PF at lower levels, ommatidia are prevented from forming, as happens in Egfr Elp mutants. At higher levels causes all retinal cells to differentiate as photoreceptors.

(Lesokhin et al., 1999)

Expression of spi^s.Scer\UAS under the control of Scer\GAL4^dpp.blk1 results in extensive outgrowth of the wing pouch.

(Nagaraj et al., 1999)

Ubiquitous expression of spi^s.Scer\UAS driven by Scer\GAL4^e22c or Scer\GAL4^69B induces ectopic rows of denticles.

(Payre et al., 1999)

In spi^s.Scer\UAS/Scer\GAL4^71B wing discs, the wing pouch and the surrounding hinge region are somewhat distorted. Males dies as pupae, and females as pharate adults. In the female wings, pigmentation and trichome density suggest that they have ectopic vein material that spans the region between L3 and L2, and between L4 and L5.

(Wessells et al., 1999)

Embryos expressing spi^s.Scer\UAS under the control of Scer\GAL4^twi.PG show a marked overproduction of eve-positive mesodermal founder cells.

(Buff et al., 1998)

Triggers ectopic neuronal differentiation in the lamina, when expression is driven by Scer\GAL4^hs.PB. Cells which were destined for apoptosis assume a proper L-neuron identity.

(Huang et al., 1998)

Scer\GAL4^55B-mediated expression causes non-autonomous effects, the production of dorsal appendages around the anterior circumference of the egg. A small fraction of the eggs are fertilised and develop into dorsalised embryos (absence or complete elimination of denticle bands and expansion of dorsal hairs).

(Sapir et al., 1998)

Scer\GAL4^bs-1348-mediated expression causes a strong extra-vein phenotype. Scer\GAL4^GMR.PF-mediated expression causes small disorganised eyes with blisters.

(Schnepp et al., 1998)

Scer\GAL4^sim.PS-mediated expression increases the number of dorsal medial cells. The implantation of one or two midline cells from these donors into wild type embryos induces a large number of additional dorsal medial cells. This increase in number is due to a trigger of additional mitoses, not by recruiting additional dorsal medial cell progenitors. Transplantation about 50 minutes after onset of gastrulation does not induce additional medial cell formation, the ability of mesodermal progenitors to respond to the spi signal appears to have already ceased after the early gastrula stage. Scer\GAL4^rho.PL-mediated expression does not increase dorsal medial cell number. Transplantation of one or two midline cells into wild type embryos induces a large number of additional dorsal medial cells.

(Luer et al., 1997)

Neither Scer\GAL4^sim.PS-mediated expression nor transplantation of single midline cells that secrete spi into the CNS midline of wild type hosts causes an increase in the number of lateral CNS neurones in each abdominal hemineuromere. Transplantation can increase the number of aCC/pCC neurons.

(Menne et al., 1997)

Embryos expressing spi^s.Scer\UAS under the control of Scer\GAL4^en-e16E have approximately two rows of ectopic denticles anterior to the denticle belts.

(O'Keefe et al., 1997)

Produces larvae with widened denticle belts and narrowed regions of naked cuticle between the belts, when expressed ubiquitously in embryos using Scer\GAL4^arm.PS. Within the denticle belts, rows 1-4 appear normal. Posterior to this, row 5 and 6 denticles are absent; they are replaced by a wide field of small denticles that appear to be of the row 1-4 type. In addition, 1-2 extra rows of similar small denticles are located anteriorly to the normal row 1.

(Szuts et al., 1997)

Scer\GAL4^btl.PS-mediated expression does not give rise to a tracheal phenotype.

(Wappner et al., 1997)

Scer\GAL4^GMR.PF induced expression causes dramatic overrecruitment of photoreceptors. Scer\GAL4^hs.PB induced expression causes a complex eye phenotype, even without heat shock: additional recruitment of photoreceptor in one area and loss of entire ommatidia in another.

(Freeman, 1996)

When driven by Scer\GAL4^Kr.PM or Scer\GAL4^rho.PL, embryos become ventralized.

(Schweitzer et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.P3.7, spi^s.UAS has abnormal neuroanatomy | embryonic stage 13 phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has abnormal neuroanatomy | embryonic stage 16 phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP7397, spi^s.UAS has increased occurrence of cell division | adult stage phenotype, non-suppressible by Ret^GD45, Scer\GAL4^NP7397

(Perea et al., 2017)

Scer\GAL4^sim.P3.7, spi^s.UAS has abnormal neuroanatomy | embryonic stage 13 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has abnormal neuroanatomy | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

NOT Enhancer of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-enhancer of abnormal neuroanatomy | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/spi^s.UAS is a suppressor of increased cell death | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

spi^s.UAS/Scer\GAL4^vn-GAL4 is a suppressor | partially of lethal - all die before end of pupal stage phenotype of vn^L6/vn^GAL4

(Austin et al., 2014)

spi^s.UAS, Scer\GAL4^Act5C.PU is a suppressor | partially of visible phenotype of DCTN1-p150^Δ.UASp, Scer\GAL4^Act5C.PU

(Iyadurai et al., 2008)

Scer\GAL4^sli.PS/spi^s.UAS is a suppressor of lethal phenotype of Scer\GAL4^sli.PS, spen^ΔC.UAS

(Chen and Rebay, 2000)

NOT Suppressor of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-suppressor of abnormal neuroanatomy | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Other

Statement

Reference

N^GD14477, Scer\GAL4^NP7397, spi^s.UAS has hyperplasia | adult stage phenotype

(Hudry et al., 2016)

Scer\GAL4^vn-GAL4, spi^s.UAS, vn^L6/vn^GAL4 has lethal - all die before end of larval stage phenotype

(Austin et al., 2014)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | embryonic stage 13 | increased number phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | embryonic stage 16 | increased number phenotype, non-enhanceable by mam^ΔC

(Zhang et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^Act5C.PP, rho^UAS.cGa, spi^s.UAS has eye photoreceptor cell | somatic clone phenotype, suppressible by rasp^T392/rasp^T802

(Miura et al., 2006)

Scer\GAL4^en-e16E, spi^s.UAS has abdominal lateral pentascolopidial chordotonal organ lch5 | increased number phenotype, suppressible by Scer\GAL4^en-e16E/salm^UAS.cKa

(Elstob et al., 2001)

Scer\GAL4^Dll-md23, spi^s.UAS has leg disc phenotype, suppressible by dpp^UAS.cSa/Scer\GAL4^Dll-md23

(Kubota et al., 2000)

Scer\GAL4^Dll-md23, spi^s.UAS has wing disc phenotype, suppressible | partially by dpp^UAS.cSa/Scer\GAL4^Dll-md23

(Kubota et al., 2000)

Scer\GAL4^dpp.blk1, spi^s.UAS has wing disc phenotype, suppressible by vg¹/vg¹

(Nagaraj et al., 1999)

Scer\GAL4^e22c, spi^s.UAS has embryonic epidermis phenotype, suppressible by ovo^svb-2

(Payre et al., 1999)

Scer\GAL4^e22c, spi^s.UAS has denticle | ectopic phenotype, suppressible by ovo^svb-2

(Payre et al., 1999)

Scer\GAL4^69B, spi^s.UAS has embryonic epidermis phenotype, suppressible by ovo^svb-2

(Payre et al., 1999)

Scer\GAL4^69B, spi^s.UAS has denticle | ectopic phenotype, suppressible by ovo^svb-2

(Payre et al., 1999)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP7397, spi^s.UAS has intestinal stem cell of posterior adult midgut epithelium phenotype, non-suppressible by Ret^GD45, Scer\GAL4^NP7397

(Perea et al., 2017)

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | increased number | embryonic stage 13 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^sim.P3.7, spi^s.UAS has midline glial cell | increased number | embryonic stage 16 phenotype, non-suppressible by mam^ΔC

(Zhang et al., 2011)

Scer\GAL4^repo, spi^s.UAS has lch5 ligament attachment cell | increased number phenotype, non-suppressible by rho^PΔ38/rho^PΔ38

(Inbal et al., 2004)

Scer\GAL4^en-e16E, spi^s.UAS has embryonic/larval oenocyte | increased number phenotype, non-suppressible by Scer\GAL4^en-e16E/salm^UAS.cKa

(Elstob et al., 2001)

NOT Enhancer of

Statement

Reference

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-enhancer of midline glial cell | embryonic stage 16 | increased number phenotype of mam^ΔC

(Zhang et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/spi^s.UAS is a suppressor | somatic clone of eye disc | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

Scer\GAL4^tin.CΔ4/spi^s.UAS is a suppressor of adult heart chamber phenotype of ru¹

(Yu et al., 2010)

spi^s.UAS, Scer\GAL4^Act5C.PU is a suppressor | partially of eye phenotype of DCTN1-p150^Δ.UASp, Scer\GAL4^Act5C.PU

(Iyadurai et al., 2008)

spi^s.UAS, Scer\GAL4^Act5C.PU is a suppressor | partially of ommatidium phenotype of DCTN1-p150^Δ.UASp, Scer\GAL4^Act5C.PU

(Iyadurai et al., 2008)

spi^s.UAS/Scer\GAL4^ato.3.6 is a suppressor of embryonic/larval oenocyte phenotype of abd-A^M1

(Brodu et al., 2002)

Scer\GAL4^en-e16E/spi^s.UAS is a suppressor of embryonic/larval oenocyte phenotype of abd-A^M1

(Brodu et al., 2002)

spi^s.UAS/Scer\GAL4^ey.PH is a suppressor | partially of photoreceptor neuron phenotype of Scer\GAL4^ey.PH, wg^UAS.cAa

(Hazelett et al., 1998)

spi^s.UAS/Scer\GAL4^ey.PH is a suppressor | partially of eye disc | posterior phenotype of Scer\GAL4^ey.PH, wg^UAS.cAa

(Hazelett et al., 1998)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/spi^s.UAS is a non-suppressor of photoreceptor | somatic clone | third instar larval stage phenotype of Vps4^3B1

(Legent et al., 2015)

spi^s.UAS/Scer\GAL4^sim.P3.7 is a non-suppressor of midline glial cell | increased number | embryonic stage 16 phenotype of mam^ΔC

(Zhang et al., 2011)

Other

Statement

Reference

N^GD14477, Scer\GAL4^NP7397, spi^s.UAS has adult posterior midgut epithelium | adult stage phenotype

(Hudry et al., 2016)

Scer\GAL4^Tub.PU, Vps4^3B1, spi^s.UAS has photoreceptor | increased number | somatic clone | cell non-autonomous | third instar larval stage phenotype

(Legent et al., 2015)

Scer\GAL4^vn-GAL4, spi^s.UAS, vn^L6/vn^GAL4 has wing vein | ectopic phenotype

(Austin et al., 2014)

Scer\GAL4^vn-GAL4, spi^s.UAS, vn^L6/vn^GAL4 has wing vein phenotype

(Austin et al., 2014)

Scer\GAL4^Act5C.PP, rho^UAS.cGa, spi^s.UAS has eye photoreceptor cell | somatic clone phenotype

(Miura et al., 2006)

Scer\GAL4^sim.P3.7, rho^PΔ38, spi^s.UAS has midline glial cell | ectopic phenotype

(Lanoue and Jacobs, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of intestinal progenitor cells in mitosis characteristic for adult flies expressing spi^s.Scer\UAS driven by Scer\GAL4^esg-NP7397 (combined with tub-Gal80[ts] for temporal control) is not suppressed by co-expression of Ret^GD45.

(Perea et al., 2017)

The adulthood-only co-expression of N^GD14477 and spi^s.UAS under the control of Scer\GAL4^esg-NP7397 (and Gal80[ts], for the temporal regulation of expression) leads to adult posterior midgut hyperplasia in males, as compared to controls.

(Hudry et al., 2016)

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf^15aΔ;Stam¹⁹ double homozygous somatic MARCM clones in third instar larval eye disc is strongly suppressed by expression of spi^s.Scer\UAS under the control of the Scer\GAL4^Act.PU driver in the mutant clones.

(Sheng et al., 2016)

Expression of spi^s.Scer\UAS does not suppress the photoreceptor differentiation defects seen in Vps4^3B1 mutant clones, but induces ectopic photoreceptor differentiation in the wild type cells surrounding the clones.

(Legent et al., 2015)

Expression of spi^s.Scer\UAS under the control of Scer\GAL4^vn.GAL4 rescues the lethality of vn^L6/vn^GAL4 mutants to the pharate adult stage. The wings have fused L3 and L4 veins and extra vein material. The wing disc phenotype is partially rescues and no leg defects are observed. The extent of the rescue depends on the level of expression, but viable flies are never seen. Very high levels of expression are toxic, with animals dying as larvae.

(Austin et al., 2014)

mam^ΔC does not suppress the extra anterior midline cells seen in stage 13 embryos when spi^s.Scer\UAS is expressed under the control of Scer\GAL4^sim.P3.7. At stage 16, embryos expressing spi^s.Scer\UAS under the control of Scer\GAL4^sim.P3.7 in a mam^ΔC mutant background contain a similar number of extra anterior midline glia cells as in either mutant alone.

(Zhang et al., 2011)

Cardiac-specific expression of spi^s.Scer\UAS driven by Scer\GAL4^tin.CΔ4 restores normal cardiac function in Df(3L)ED4238/+ mutants.

Cardiac-specific expression of spi^s.Scer\UAS driven by Scer\GAL4^tin.CΔ4 restores normal cardiac function in homozygous ru¹ mutants.

(Yu et al., 2010)

The rough eye phenotype caused by expression of Gl^{Δ.Scer\UAS.P\T} under the control of two copies of Scer\GAL4^Act5C is significantly suppressed by coexpression of spi^s.Scer\UAS.

(Iyadurai et al., 2008)

Coexpression of spi^s.Scer\UAS and Krn^{s.Scer\UAS.cUa}, under the control of Scer\GAL4^cb41, in follicle cell clones results in a higher penetrance of the border cell misguidance phenotype compared to expression of either transgene alone, although the effect is additive rather than synergistic.

(McDonald et al., 2006)

Somatic clones of Scer\GAL4^Act5C.PP cells in the eye disc in animals carrying rho^Scer\UAS.cGa and spi^s.Scer\UAS cause ectopic photoreceptor differentiation. This phenotype is completely suppressed by rasp^T392/rasp^T802.

(Miura et al., 2006)

Expression of spi^s.Scer\UAS under the control of Scer\GAL4^en-e16E or Scer\GAL4^ato.3.6 rescues the formation of oenocytes in abd-A^M1 mutant embryos.

(Brodu et al., 2002)

Coexpression of spi^s.Scer\UAS and salm^Scer\UAS.cKa under the control of Scer\GAL4^en-e16E results in a chordotonal phenotype identical to that seen when salm^Scer\UAS.cKa alone is expressed under the control of Scer\GAL4^en-e16E. Giant clusters of oenocyte cells are induced dorsally, as is seen when spi^s.Scer\UAS alone is expressed under the control of Scer\GAL4^en-e16E.

(Elstob et al., 2001)

Co-expression of dpp^Scer\UAS.cSa partially restores the formation of wing discs in embryos expressing spi^s.Scer\UAS under the control of Scer\GAL4^Dll-md23 and rescues the leg disc phenotype of these embryos.

(Kubota et al., 2000)

Addition of spi^s.Scer\UAS when driven by Scer\GAL4^sim.P3.7 in a rho^PΔ38 background produces double the number of midline glial cells seen compared to wild-type.

(Lanoue and Jacobs, 1999)

There is no expansion of the wing pouch in animals expressing spi^s.Scer\UAS under the control of Scer\GAL4^dpp.blk1 if they are also homozygous for vg¹.

(Nagaraj et al., 1999)

The ectopic rows of denticles induced by expression of spi^s.Scer\UAS driven by Scer\GAL4^e22c or Scer\GAL4^69B are suppressed by ovo^svb-2.

(Payre et al., 1999)

Photoreceptor differentiation and growth is weakly rescued in flies expressing wg^Scer\UAS.cAa under the control of Scer\GAL4^ey.PH if they are also co-expressing spi^s.Scer\UAS.

(Hazelett et al., 1998)

Homozygous wg^l-17 larvae derived from embryos in which spi^s.Scer\UAS is expressed under the control of Scer\GAL4^arm.PS have lawns of denticles; these denticles are clearly smaller than those present in homozygous wg^l-17 larvae that do not carry spi^s.Scer\UAS, and probably correspond to row 1-4 denticles. Homozygous arm² larvae derived from embryos in which spi^s.Scer\UAS is expressed under the control of Scer\GAL4^arm.PS have denticle lawns composed of small denticles.

(Szuts et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The normal protocol for creating transgenic flies caused excessive embryonic lethality. Reducing the concentration of injected DNA to 0.2mg/ml allowed 7% embryonic survival. Injection of the same coding region driven by an Hsp70 promoter results in 100% embryonic lethality.

(Schweitzer et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

spi^s.Scer\UAS

spi^s.UAS

spitz^secreted

(Matzat et al., 2015)

Name Synonyms

secreted

(Schweitzer et al., 1995)

Secondary FlyBase IDs

References (91)

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