FlyBase Allele Report: Dmel\drk[e0A]

General Information

Symbol

Dmel\drk^e0A

Species

D. melanogaster

Name

FlyBase ID

FBal0287941

Feature type

allele

Associated gene

Dmel\drk

Associated Insertion(s)

Carried in Construct

Also Known As

drk^eOA

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Cooper et al., 1996)

Mutagen

ethyl methanesulfonate

(Simon et al., 1993)

Progenitor genotype

Cytology

Description

Amino acid replacement: H106Y.

(Moressis et al., 2009)

Amino acid replacement: H106Y. H106 is a highly conserved residue among SH2 domains.

(Olivier et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:13,497,514..13,497,514 [-]

Nucleotide change:

C13497514T

Amino acid change:

Reported amino acid change:

H106Y

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Moressis et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of autosomal recessive nonsyndromic deafness 114

CEA with drk⁶

(Li et al., 2019)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates autosomal recessive intellectual developmental disorder

modeled by Nab2^EP3716

(Rounds et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning

(Moressis et al., 2009)

lethal

(Li et al., 2019)

lethal (with drk^ΔP24)

(Li et al., 2019)

lethal | recessive

(Cooper et al., 1996, Simon et al., 1993, Simon et al., 1991)

lethal - all die before end of pupal stage (with drk^R1)

(Olivier et al., 1993)

partially lethal - majority live (with drk⁶)

(Li et al., 2019)

partially lethal - majority live (with drk^14-7)

(Li et al., 2019)

some die during pupal stage (with drk^R1)

(Olivier et al., 1993)

visible | dominant

(Cooper et al., 1996)

Phenotype Manifest In

photoreceptor cell R7

(Cooper et al., 1996)

Detailed Description

Statement

Reference

drk⁶/drk^e0A transheterozygotes exhibit disorganized scolopidia (as revealed by phalloidin staining, which labels cap rods, scolopale rods, and the actin bundles within the cilia of the mechanosensory neurons).

(Li et al., 2019)

Olfactory association learning is lower in drk^e0A heterozygotes compared to controls.

Histological and immunohistochemical examination of drk^e0A heterozygous heads with multiple antigenic markers does not reveal and gross structural anomalies in the brain. Avoidance of odors used as CS and electroshock (US) are no different from that of controls. Heterozygous mutants exhibit normal odor avoidance after pre-exposure to electric shock.

(Moressis et al., 2009)

Mutation disrupts development of receptor cell R7.

(Cooper et al., 1996)

Reduces the ability of a cell to develop as an R7 cell or as any other photoreceptor.

(Simon et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

drk^e0A has abnormal learning phenotype, suppressible by Scer\GAL4^c747/Raf^fl.UAS

(Moressis et al., 2009)

drk^e0A has abnormal learning phenotype, suppressible by Scer\GAL4^c747/Raf^UAS.F179

(Moressis et al., 2009)

Enhancer of

Statement

Reference

drk[+]/drk^e0A is an enhancer of visible | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Rounds et al., 2022)

drk[+]/drk^e0A is an enhancer of abnormal eye color phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Rounds et al., 2022)

drk[+]/drk^e0A is an enhancer of abnormal size | adult stage phenotype of sl²

(Murillo-Maldonado et al., 2011)

NOT Enhancer of

Statement

Reference

drk[+]/drk^e0A is a non-enhancer of abnormal size | adult stage phenotype of sl⁹

(Murillo-Maldonado et al., 2011)

drk^e0A is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

drk^e0A is a non-enhancer of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

drk^e0A is a non-enhancer of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

drk[+]/drk^e0A is a suppressor of increased cell number | adult stage phenotype of sl²

(Murillo-Maldonado et al., 2011)

drk[+]/drk^e0A is a suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

drk[+]/drk^e0A is a suppressor of visible phenotype of Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B

(MacDougall et al., 2004)

drk[+]/drk^e0A is a suppressor of visible phenotype of Src64B^Δ540.hs.sev

(Cooper et al., 1996)

NOT Suppressor of

Statement

Reference

drk^e0A is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

drk^e0A is a non-suppressor of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

drk^e0A is a non-suppressor of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

drk^e0A is a non-suppressor of visible phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

drk^e0A is a non-suppressor of visible phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^dpp.blk1

(Ishimaru et al., 1999)

Phenotype Manifest In

Enhancer of

Statement

Reference

drk[+]/drk^e0A is an enhancer of eye phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Rounds et al., 2022)

drk[+]/drk^e0A is an enhancer of wing blade phenotype of sl²

(Murillo-Maldonado et al., 2011)

drk^e0A is an enhancer of larval longitudinal connective phenotype of Ggal\Cam^{KA.ftz.Tag:MT(Khc)}

(Fritz and VanBerkum, 2000)

drk^e0A is an enhancer of phenotype of svp^hs.ro1

(Kramer et al., 1995)

drk^e0A is an enhancer of photoreceptor cell R7 phenotype of sev^B4

(Simon et al., 1993)

NOT Enhancer of

Statement

Reference

drk[+]/drk^e0A is a non-enhancer of wing blade phenotype of sl⁹

(Murillo-Maldonado et al., 2011)

Scer\GAL4^Tub.PU/drk^e0A is a non-enhancer of wing vein phenotype of Scer\GAL4^Tub.PU, csw^Y279C.UASp

(Oishi et al., 2009)

drk^e0A is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

drk^e0A is a non-enhancer of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

drk^e0A is a non-enhancer of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

drk[+]/drk^e0A is a suppressor of wing vein | ectopic phenotype of sl²

(Murillo-Maldonado et al., 2011)

drk[+]/drk^e0A is a suppressor of photoreceptor cell R7 | adult stage phenotype of sl²

(Murillo-Maldonado et al., 2011)

drk[+]/drk^e0A is a suppressor of dorsal appendage phenotype of Cbl^L.UAS, Scer\GAL4^EQ1

(Wang and Pai, 2011)

drk[+]/drk^e0A is a suppressor of dorsal appendage phenotype of Cbl^L.Δ70Z.UAS, Scer\GAL4^EQ1

(Wang and Pai, 2011)

drk[+]/drk^e0A is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

drk[+]/drk^e0A is a suppressor of wing vein | ectopic phenotype of Pi3K68D^{KD.UAS.Tag:MYC}, Scer\GAL4^69B

(MacDougall et al., 2004)

drk^e0A is a suppressor of photoreceptor cell R1 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

drk^e0A is a suppressor of photoreceptor cell R2 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

drk^e0A is a suppressor of photoreceptor cell R3 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

drk^e0A is a suppressor of photoreceptor cell R4 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

drk^e0A is a suppressor of photoreceptor cell R5 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

drk^e0A is a suppressor of photoreceptor cell R6 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

drk[+]/drk^e0A is a suppressor of eye phenotype of Src64B^Δ540.hs.sev

(Cooper et al., 1996)

drk^e0A is a suppressor of phenotype of Src42A^KR.hs.2sev

(Takahashi et al., 1996)

drk^e0A is a suppressor of phenotype of svp^1.sev

(Begemann et al., 1995)

drk^e0A is a suppressor of phenotype of tor^11D

(Doyle and Bishop, 1993)

NOT Suppressor of

Statement

Reference

drk[+]/drk^e0A is a non-suppressor of dorsal appendage phenotype of Cbl^L.mPR.UAS, Scer\GAL4^EQ1

(Wang and Pai, 2011)

Scer\GAL4^Tub.PU/drk^e0A is a non-suppressor of wing vein phenotype of Scer\GAL4^Tub.PU, csw^Y279C.UASp

(Oishi et al., 2009)

drk^e0A is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

drk^e0A is a non-suppressor of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

drk^e0A is a non-suppressor of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

drk^e0A is a non-suppressor of eye phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

drk^e0A is a non-suppressor of wing vein L3 phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^dpp.blk1

(Ishimaru et al., 1999)

drk^e0A is a non-suppressor of photoreceptor cell R7 phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Other

Statement

Reference

da², drk[+]/drk^e0A has follicle cell phenotype

(Smith et al., 2002)

da², drk^e0A has follicle cell phenotype

(Smith et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

dock⁰⁴⁷²³ drk^e0A double mutant embryos do not show defects in myoblast fusion.

(Kaipa et al., 2013)

One copy of drk^e0A enhances the reduction in wing blade area seen in homozygous sl² males.

One copy of drk^e0A does not enhance the reduction in wing blade area seen in homozygous sl⁹ males.

One copy of drk^e0A partially suppresses the ectopic wing vein phenotype seen in sl² homozygotes.

One copy of drk^e0A partially suppresses the percentage of sl² mutant ommatidia that contain extra R7 photoreceptors.

(Murillo-Maldonado et al., 2011)

drk^e0A/+ significantly suppresses the ventralised egg shell phenotype observed when Cbl^L.Scer\UAS is expressed in follicle cells under the control of Scer\GAL4^EQ1.

drk^e0A/+ fails to suppresses the ventralised egg shell phenotype observed when Cbl^{L.mPR.Scer\UAS} is expressed in follicle cells under the control of Scer\GAL4^EQ1.

The dorsalised eggshell phenotype observed when Cbl^{L.Δ70Z.Scer\UAS} is expressed under the control of Scer\GAL4^EQ1 is suppressed in a drk^e0A/+ background.

(Wang and Pai, 2011)

Expression of phl^fl.Scer\UAS under the control of Scer\GAL4^c747 results in full reversal of the learning deficit of drk^e0A heterozygotes.

Expression of phl^{Scer\UAS.F179} under the control of Scer\GAL4^c747 results in full reversal of the learning deficit of drk^e0A heterozygotes.

(Moressis et al., 2009)

The presence of a drk^e0A background does not affect ectopic wing vein formation in csw^{Y279C.Scer\UAS} (Scer\GAL4^tub) mutants.

(Oishi et al., 2009)

drk^e0A/+ suppresses the ectopic wing vein phenotype caused by expression of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^69B.

(MacDougall et al., 2004)

Weakly enhances the eye phenotype produced by activated arm constructs. (either arm^S44Y.GMR or arm^S56F.GMR).

(Freeman and Bienz, 2001)

Has no effect on the eye phenotype caused by C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} expressed under the control of Scer\GAL4^GMR.PF. Has no effect on the wing vein phenotype caused by C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} expressed under the control of Scer\GAL4^dpp.blk1.

(Ishimaru et al., 1999)

drk^e0A significantly suppresses the formation of extra outer photoreceptors caused by B-H1^sev.PH, although it does not affect the loss of R7 cell phenotype caused by B-H1^sev.PH.

(Hayashi et al., 1998)

Presence of the mutation in Src64B^Δ540.hs.sev flies alleviates the rough eye phenotype.

(Cooper et al., 1996)

No effect on the faf eye phenotype.

(Huang and Fischer-Vize, 1996)

Suppressor of the dosage-dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors.

(Begemann et al., 1995)

Mutation has no effect on the rough eye phenotype caused by two insertions of P{GMR-Rho1}.

(Hariharan et al., 1995)

Suppression of the rough eye phenotype (formation of extra R7 cells) caused by P{sev-svp2} and enhancement of the P{ro-svp1} rough eye phenotype (loss of one or more outer photoreceptors from many of the ommatidia).

(Kramer et al., 1995)

Weak suppressor of tor^11D phenotype, embryos display 2 to 3 abdominal denticle belts.

(Doyle and Bishop, 1993)

In drk^E(sev)2B sev^B4 combination adults nearly all of the ommatidia lack the R7 cell. The presence of P{sev-drk} restores an R7 cell to most ommatidia. Homozygous drk^E(sev)2B clones in P{sevRas1.V12} flies frequently develop as photoreceptors despite the lack of drk function. The rescued cells can be inner R1-R6-like photoreceptors or outer R7-like photoreceptors.

(Simon et al., 1993)

Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling.

(Simon et al., 1991)

Xenogenetic Interactions

Statement

Reference

The penetrance of the Fas2-positive axon crossover phenotype seen in embryos carrying Khc::Ggal\MLCK^KA.ftz is increased if they are also homozygous for drk^e0A.

(Fritz and VanBerkum, 2000)

Complementation and Rescue Data

Partially complements

drk⁶

(Li et al., 2019)

drk^14-7

(Li et al., 2019)

Rescued by

drk^e0A is rescued by drk^hs.PM

(Moressis et al., 2009)

Comments

The lethality seen in drk^R1/drk^E(sev)2B transheterozygotes can be rescued by drk product supplied under a heat shock promoter.

(Olivier et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (2)

5691

108368

Notes on Origin

Discoverer

Comments

The recessive lethal mutation, "l(2)E(sev)2B", is the same lesion as drk^E(sev)2B.

(Simon et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

Drk^EOA

(Wang and Pai, 2011)

Drk^H106Y

(Cooper et al., 1996)

E(sev)2B^e0A

(Takahashi et al., 1996)

drk^E(sev)2B

drk^E0A

(Moressis et al., 2009, Huang and Fischer-Vize, 1996)

drk^e0A

(Rounds et al., 2022, Li et al., 2019, Kaipa et al., 2013, Murillo-Maldonado et al., 2011, Oishi et al., 2009, Oishi et al., 2006, MacDougall et al., 2004)

drk^eOA

(Tsarouhas et al., 2014, Smith et al., 2002)

drk^eOa

(Schweitzer et al., 1995)

drk^eoa

(Fritz and VanBerkum, 2000)

Name Synonyms

Secondary FlyBase IDs

FBal0031081
FBal0031079

References (30)

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