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FB2026_01
,
released March 12, 2026
F
G-protein coupled receptor for Sevenless - regulates determination of R7 photoreceptor fate - responds to extracellular glucose levels in order to regulate energy homeostasis
Please see the JBrowse view of Dmel\boss for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Gene model reviewed during 5.53
3.2 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
96 (kD)
896 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\boss using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
The boss transcript is expressed at a level at least 100-fold greater in poly(A) RNA isolated from head than in that isolated from total disc tissue.
bossEX protein was localized to the apical membranes of receptor cells, cone cells and the mystery cell. It was also found in a perinuclear region of all cells in the developing eye disc in a pattern consistent with localization to the endoplasmic reticulum.
boss1TM protein is targeted to the plasma membrane in S2 cells but accumulates in the perinuclear endoplasmic reticulum in eye discs. boss1TM protein is localized apically in FBgn0003366:sev @-expressing cells.
In the eye imaginal disc, boss protein is first expressed in cells lying three rows posterior to the morphogenetic furrow and is restricted to R8 cells. Later boss protein is observed within multivesicular bodies in R7 cells. In later stages of eye development, boss protein is more broadly expressed. About 24 hours after puparium formation, it is expressed in the bristle neurons of the retina. About 24 hours later, it is expressed in all photoreceptor cells in the retina. boss protein expression is also observed in many sensory neurons in the antenna and leg discs and in the ocelli.
bosshs.P is strongly expressed on the apical membranes of all cells anterior and posterior to the morphogenetic furrow.
JBrowse - Visual display of RNA-Seq signals
View Dmel\boss in JBrowseMaps just proximal to Pr.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
boss functions as a glucose-responding receptor.
One of a class of genes with TATA-less promoters that have the conserved DPE sequence.
In vitro studies show that boss activates tyrosine phosphorylation of the sev receptor and the transmembrane domain of boss is necessary for its function. A soluble form of boss acts as an antagonist of the sev receptor both in vivo and in vitro.
Functional interchangeability of D.melanogaster and D.virilis sev and boss were demonstrated using chimeric genes. Noncoding sequences which may regulate boss expression were identified based on sequence conservation during evolution.
Antibody staining demonstrates that the entire boss protein is internalized by sev-expressing tissue culture cells and by the R7 precursor cell in the developing eye imaginal disc demonstrating the receptor mediated transfer of a transmembrane ligand.
Some aspect of R7 differentiation is independent of the genetic pathway involving sev, boss and sina as some P{HZ}klgH214 expression is present in R7 of boss mutants.
Double mutant flies with sevS11.T:Hsap\MYC demonstrate that the recruitment of multiple ommatidial precursors into the R7 developmental pathway is independent of the boss pathway.
The R8 photoreceptor neuron induces a neighbouring cell to assume an R7 cell fate. The induction is mediated by two transmembrane proteins encoded by sev and boss.
Homozygotes lack photoreceptor cell R7; phenotype indistinguishable from that of sev. Mosaic studies demonstrate that boss+ activity required in R8 and in no other photoreceptor cell for the normal development of the R7 cell in the same ommatidium. boss+ R8 cell cannot rescue R7 development in adjacent ommatidia.
