FlyBase Gene Report: Dmel\MBD-like

General Information

Symbol

Dmel\MBD-like

Species

D. melanogaster

Name

Methyl-CpG binding domain protein-like

Annotation Symbol

CG8208

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027950

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

Methyl-CpG binding domain protein-like (MBD-like) encodes a protein involved in chromosome condensation and transcription repression. [Date last reviewed: 2019-08-01] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

dMBD2/3, MBD2/3, MBD3, MBD2

Key Links

Genomic Location

Cytogenetic map

85D24-85D24

Sequence location

3R:9,559,760..9,561,533 [+]

Recombination map

3-48

RefSeq locus

NT_033777 REGION:9559760..9561533

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

enables histone deacetylase binding

inferred from physical interaction with FLYBASE:HDAC1; FB:FBgn0015805

(Ballestar et al., 2001, Tweedie et al., 1999)

enables methyl-CpG binding

inferred from direct assay

(Roder et al., 2000)

enables protein binding

inferred from physical interaction with FLYBASE:Caf1-55; FB:FBgn0263979

(Marhold et al., 2004)

enables protein homodimerization activity

inferred from physical interaction with FLYBASE:MBD-like; FB:FBgn0027950

(Marhold et al., 2004)

enables transcription corepressor activity

inferred from direct assay

(Ballestar et al., 2001)

NOT enables methyl-CpG binding

inferred from direct assay

(Ballestar et al., 2001, Tweedie et al., 1999)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables DNA binding

inferred from electronic annotation with InterPro:IPR001739, InterPro:IPR016177

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR016177

(InterPro Project Members, 2004-)

enables methyl-CpG binding

inferred from biological aspect of ancestor with PANTHER:PTN000963505

(GO Reference Genome Project, 2011-)

NOT enables methyl-CpG binding

traceable author statement

(Hendrich and Tweedie, 2003)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in chromosome condensation

inferred from mutant phenotype

(Nikalayevich and Ohkura, 2015)

involved_in negative regulation of DNA-templated transcription

inferred from direct assay

(Ballestar et al., 2001, Roder et al., 2000)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in DNA methylation-dependent constitutive heterochromatin formation

inferred from biological aspect of ancestor with PANTHER:PTN000963505

(GO Reference Genome Project, 2011-)

involved_in negative regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000963505

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in nucleus

inferred from physical interaction with FLYBASE:HDAC1; FB:FBgn0015805

(Tweedie et al., 1999)

inferred from physical interaction with FLYBASE:Mi-2; FB:FBgn0262519

(Tweedie et al., 1999)

part_of NuRD complex

inferred from physical interaction with FLYBASE:Mi-2; FB:FBgn0262519

(Ballestar et al., 2001, Tweedie et al., 1999)

inferred from physical interaction with FLYBASE:HDAC1; FB:FBgn0015805

(Ballestar et al., 2001)

located_in polytene chromosome

inferred from direct assay

(Ballestar et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000963505

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

NUCLEOSOME REMODELING DEACETYLASE COMPLEX

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Methyl-CpG binding domain protein-like (MBD-like) encodes a protein involved in chromosome condensation and transcription repression. [Date last reviewed: 2019-08-01]

Gene Group (FlyBase)

NUCLEOSOME REMODELING DEACETYLASE COMPLEX -: The nucleosome remodelling and deacetylase (NuRD) complex couples chromatin remodelling and histone deacetylation to mediate transcriptional repression. (Adapted from FBrf0228973 and FBrf0228920).

Summary (Interactive Fly)

methyl-DNA binding protein involved in methylation dependent transcriptional repression - co-repressor that targets the MI-2 complex to methylated DNA

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\MBD-like for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A0B4KGZ0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

MBD-like-RA

FBtr0082069

NM_169293

1222

339

MBD-like-RB

FBtr0082068

NM_141650

883

226

MBD-like-RC

FBtr0334654

NM_001275492

1225

340

Additional Transcript Data and Comments

Reported size (kB)

1.2, 1 (northern blot)

(Marhold et al., 2002)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

MBD-like-PA

FBpp0081547

36.3

339

10.57

FBpp0081546

25.3

226

10.44

FBpp0306716

36.4

340

10.57

A0A0B4KGZ0

NP_001262421

AGB95803

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

30, 25 (kD)

(Marhold et al., 2002)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MBD-like using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

MBD-like_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.65

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | faint | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

testis

(Marhold et al., 2002)

ovary

(Marhold et al., 2002)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

• cytoplasm

(Marhold et al., 2002)

embryonic stage 5

• cytoplasm

(Marhold et al., 2002)

adult stage

primary gonial cell

• cytoplasm

(Marhold et al., 2002)

spermatogonium

• cytoplasm

(Marhold et al., 2002)

spermatocyte

• nucleus

(Marhold et al., 2002)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 15

Comment: reference states 0-12 hr AEL

(Marhold et al., 2002)

embryonic stage 11 -- 17

Comment: reference states 6-20 hr AEL

(Marhold et al., 2002)

Additional Descriptive Data

Developmental western analysis shows that the long MBD-like isoform is present in embryonic extracts from 0-12 hours of development.

(Marhold et al., 2002)

Developmental western analysis shows that the short MBD-like isoform is present in embryonic extracts from 6-20 hours of development.

(Marhold et al., 2002)

Homogenous expression of MBD-like protein is observed in cleavage embryos. The staining intensity increases and staining is cytoplasmic. At cellular blastoderm stage there is a transient colocalization of the protein and DNA indicating a nuclear localization of MBD-like protein at this stage. However in gastrula and later embryonic development protein staining is detected primarily in the cytoplasm and nuclear periphery. Nuclear accumulation of MBD-like protein coincides with zygotic genome activation. Nuclear MBD-like protein was also observed in primary spermatocytes at the time of Y-chromosome activation. The protein was predominately cytoplasmic at earlier stages of spermatogenesis.

(Marhold et al., 2002)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from physical interaction with FLYBASE:HDAC1; FB:FBgn0015805

(Tweedie et al., 1999)

inferred from physical interaction with FLYBASE:Mi-2; FB:FBgn0262519

(Tweedie et al., 1999)

part_of NuRD complex

inferred from physical interaction with FLYBASE:Mi-2; FB:FBgn0262519

(Ballestar et al., 2001, Tweedie et al., 1999)

inferred from physical interaction with FLYBASE:HDAC1; FB:FBgn0015805

(Ballestar et al., 2001)

located_in polytene chromosome

inferred from direct assay

(Ballestar et al., 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\MBD-like in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\MBD-like

View exonic expression by tissue for Dmel\MBD-like

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027950

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41151/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027950

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027950

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027950

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8208

Flygut - An atlas of the Drosophila adult midgut

FBgn0027950

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of MBD-like

Transgenic constructs containing regulatory region of MBD-like

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3R)Exel6153

(Ryder, 2004.4.26)

Df(3R)Exel6264

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

MBD-like^EY04582

viable, with Scer\GAL4^Mef2.PR

MBD-like^GD1306

viable, with Scer\GAL4^pnr-MD237

MBD-like^GD1306

viable, with Scer\GAL4^tin.CΔ4

MBD-like^GD1306

Sterility

Allele

fertile

MBD-like^EY04582

Other Phenotypes

Allele

abnormal cell adhesion | oogenesis, with Scer\GAL4^slbo.2.6

MBD-like^HMS02825

abnormal cell migration | oogenesis, with Scer\GAL4^slbo.2.6

MBD-like^HMS02825

decreased body size | embryonic stage

MBD-like^EY04582

suppressor of variegation, with In(1)w^m4h

MBD-like^EP1112

suppressor of variegation | dominant, with In(1)w^m4h

MBD-like^EY04582

Phenotype manifest in

Allele

border follicle cell, with Scer\GAL4^slbo.2.6

MBD-like^HMS02825

chromosome | adult stage | female, with Scer\GAL4^{VP16.mat.αTub67C}

MBD-like^HMS02825

mitotic anaphase & nuclear chromosome

MBD-like^EY04582

oocyte, with Scer\GAL4^{VP16.mat.αTub67C}

MBD-like^HMS02825

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (11)

Hsap\MBD2

13 of 14

Yes

Hsap\MBD3

13 of 14

Yes

Hsap\MBD3L1

6 of 14

Yes

Hsap\MBD1

4 of 14

Yes

Hsap\MBD3L2

3 of 14

Yes

Hsap\MBD3L3

3 of 14

Yes

Hsap\MBD3L4

3 of 14

Yes

Hsap\MBD3L5

3 of 14

Yes

Hsap\MECP2

3 of 14

Yes

Hsap\MBD3L2B

2 of 14

Yes

Hsap\MBD4

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (7)

Rnor\Mbd2

13 of 14

Yes

Rnor\Mbd3

13 of 14

Yes

Rnor\Mbd3l1

5 of 14

Yes

Rnor\Mbd1

4 of 14

Yes

Rnor\Mbd4

2 of 14

Yes

Rnor\Mecp2

2 of 14

Yes

Rnor\Mbd3l2

1 of 14

Yes

Mus musculus (laboratory mouse) (7)

Mmus\Mbd2

13 of 14

Yes

Mmus\Mbd3

13 of 14

Yes

Mmus\Mbd1

4 of 14

Yes

Mmus\Mbd3l1

4 of 14

Yes

Mmus\Mecp2

3 of 14

Yes

Mmus\Mbd3l2

2 of 14

Yes

Mmus\Mbd4

1 of 14

Xenopus tropicalis (Western clawed frog) (7)

Xtro\mbd3

10 of 13

Yes

Xtro\mbd2

7 of 13

Yes

Xtro\mbd1

2 of 13

Yes

Xtro\dbp

1 of 13

Xtro\gapdh

1 of 13

Xtro\mbd4

1 of 13

Yes

Xtro\mecp2

1 of 13

Yes

Danio rerio (Zebrafish) (7)

Drer\mbd3a

13 of 14

Yes

Drer\mbd3b

12 of 14

Yes

Drer\mbd2

7 of 14

Yes

Drer\mbd1b

3 of 14

Yes

Drer\mecp2

3 of 14

Yes

Drer\mbd1a

1 of 14

Yes

Drer\phf20l1

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\mbd-2

6 of 14

Yes

Anopheles gambiae (African malaria mosquito) (2)

Agam\AgaP_AGAP003982

11 of 12

Yes

Agam\AgaP_AGAP006428

1 of 12

Arabidopsis thaliana (thale-cress) (8)

Atha\MBD02

2 of 13

Yes

Atha\MBD5

2 of 13

Yes

Atha\MBD6

2 of 13

Yes

Atha\MBD7

2 of 13

Yes

Atha\MBD12

2 of 13

Yes

Atha\MBD1

1 of 13

Yes

Atha\MBD3

1 of 13

Yes

Atha\MBD4

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:MBD-like. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (1)

MBD-R2

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 4 )

Human Ortholog

Disease

Evidence

References

MECP2; methyl-CpG binding protein 2

model of X-linked intellectual disability-psychosis-macroorchidism syndrome

IEA

(FlyBase, 2019-)

model of Rett syndrome

IEA

(FlyBase, 2019-)

model of syndromic X-linked intellectual disability Lubs type

IEA

(FlyBase, 2019-)

model of severe congenital encephalopathy due to MECP2 mutation

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MBD2; methyl-CpG binding domain protein 2

13 of 14

603547

MBD3; methyl-CpG binding domain protein 3

13 of 14

603573

MBD3L1; methyl-CpG binding domain protein 3 like 1

6 of 14

607963

MBD1; methyl-CpG binding domain protein 1

4 of 14

156535

MBD3L2; methyl-CpG binding domain protein 3 like 2

3 of 14

607964

MBD3L3; methyl-CpG binding domain protein 3 like 3

3 of 14

MBD3L4; methyl-CpG binding domain protein 3 like 4

3 of 14

MBD3L5; methyl-CpG binding domain protein 3 like 5

3 of 14

MECP2; methyl-CpG binding protein 2

3 of 14

METHYL-CpG-BINDING PROTEIN 2; MECP2

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

MBD-like - CDK2AP1

experimental knowledge based

(Guruharsha et al., 2011)

MBD-like - Caf1-55

anti tag coimmunoprecipitation, Identification by mass spectrometry, molecular sieving, molecular weight estimation by staining

(Zhang et al., 2016)

MBD-like - EcR

two hybrid array

(Shokri et al., 2019)

MBD-like - Eip78C

two hybrid array

(Shokri et al., 2019)

MBD-like - HDAC1

experimental knowledge based, molecular sieving, molecular weight estimation by staining

(Zhang et al., 2016, Guruharsha et al., 2011)

MBD-like - MEP-1

anti bait coimmunoprecipitation, Identification by mass spectrometry

(Reddy et al., 2010)

MBD-like - MTA1-like

anti tag coimmunoprecipitation, molecular weight estimation by staining, experimental knowledge based, ion exchange chromatography, molecular sieving, western blot

(Zhang et al., 2016, Guruharsha et al., 2011, Ballestar et al., 2001)

MBD-like - Mi-2

anti tag coimmunoprecipitation, western blot, ion exchange chromatography, molecular sieving, anti bait coimmunoprecipitation, Identification by mass spectrometry

(Fasulo et al., 2012, Reddy et al., 2010, Ballestar et al., 2001)

MBD-like - Odj

two hybrid array

(Shokri et al., 2019)

MBD-like - ecd

two hybrid array

(Shokri et al., 2019)

MBD-like - kn

two hybrid array

(Shokri et al., 2019)

MBD-like - otp

two hybrid array

(Shokri et al., 2019)

MBD-like - ttk

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

MBD-like - wor

two hybrid array

(Shokri et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

66310

DroID - A comprehensive database of gene and protein interactions.

FBgn0027950

MIST (protein-protein) - An integrated Molecular Interaction Database

41151

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

ATP-dependent chromatin remodeling

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

85D24-85D24

Sequence location

3R:9,559,760..9,561,533 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

85D24-85D24

Limits computationally determined from genome sequence between P{PZ}ps¹⁰⁶¹⁵&P{PZ}Ras85D⁰⁶⁶⁷⁷ and P{EP}CG9393^EP3122

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-46.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

15753

y¹ w^67c23; P{EPgy2}MBD-like^EY04582

35347

y¹ sc^* v¹ sev²¹; P{TRiP.GL00259}attP2

80175

y¹ sc^* v¹ sev²¹; P{TOE.GS02113}attP40

38239

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01683}attP40

44105

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02825}attP40

NIG-Fly

HMJ21420

y¹ v¹; P{TRiP.HMJ21420}attP40/CyO

GL00259

y¹ sc^* v¹ sev²¹; P{TRiP.GL00259}attP2

HMS02825

y¹ sc^* v¹ sev²¹; P{TRiP.HMS02825}attP40/CyO

VDRC

v107151

P{KK100936}VIE-260B

v9261

w¹¹¹⁸; P{GD1306}v9261

v341363

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00420}attP40/CyO-GFP

v341432

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00499}attP40/CyO-GFP

More stocks available...

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (46)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD22928

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027950

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027950

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Ballestar et al., 2001)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days.

(Claridge-Chang et al., 2001)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: methyl-CpG-binding-domain-like-protein MBD2-3

(FlyBase, 1996-)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

CG8208

(Ni et al., 2011.7.19, Ni et al., 2010.12.1, Reddy et al., 2010, Keleman et al., 2009.8.5, Kim et al., 2004, Claridge-Chang et al., 2001)

Dm Mbd2/3

(Hendrich and Tweedie, 2003)

DmMbd2/3

(Hendrich and Tweedie, 2003)

MBD-like

(Jiang et al., 2019, Raza et al., 2019, Shokri et al., 2019, Gene Disruption Project members, 2018-, Transgenic RNAi Project members, 2017-, Dietz et al., 2015, Nikalayevich and Ohkura, 2015, Rogers et al., 2014, Reddy et al., 2010, van Steensel et al., 2010, de Wit et al., 2008, Jamieson et al., 2006)

MBD2

(Schuettengruber et al., 2011, Clapier and Cairns, 2009, Bouazoune and Brehm, 2006)

MBD2-3

MBD2/3

(Bilinovich et al., 2025, Fasulo et al., 2012, Shevelyov et al., 2009, Lyko et al., 2004, Marhold et al., 2004, Marhold et al., 2004, Rasheva et al., 2003, Lyko, 2002)

MBD3

(Schuettengruber et al., 2011, Clapier and Cairns, 2009, Bajpe et al., 2008, Bouazoune and Brehm, 2006)

dMBD-like

(Ballestar et al., 2001)

dMBD2/3

(Singh et al., 2025, Mu et al., 2021, Vidaurre and Chen, 2021, Yang et al., 2020, Gupta et al., 2016, Marhold et al., 2002, Barlow et al., 2001, Lyko, 2001, Zbylut et al., 2001, Roder et al., 2000, Tweedie et al., 1999)

dMbD2/3

(Eberharter and Becker, 2004)

Name Synonyms

MBD-like

(Jamieson et al., 2006)

Methyl-CpG binding domain protein-like

methyl-CpG-binding-domain-like-protein

Secondary FlyBase IDs

FBgn0029156

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 52 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

41151

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A0B4KGZ0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A0B4KGZ0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027950

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

41151/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027950

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027950

FlyMine - An integrated database for Drosophila genomics

FBgn0027950

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8208

MARRVEL_MODEL - MARRVEL (model organism gene)

41151

Linkouts

BioGRID - A database of protein and genetic interactions.

66310

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027950

DroID - A comprehensive database of gene and protein interactions.

FBgn0027950

DRSC - Results frm RNAi screens

FBgn0027950

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

MBD-like_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027950

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027950

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8208

Flygut - An atlas of the Drosophila adult midgut

FBgn0027950

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC003775

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

ATP-dependent chromatin remodeling

MIST (protein-protein) - An integrated Molecular Interaction Database

41151

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (75)

The following external sites may use different assemblies or annotations than FlyBase.

1.65

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