FlyBase Gene Report: Dmel\rols

General Information

Symbol

Dmel\rols

Species

D. melanogaster

Name

rolling pebbles

Annotation Symbol

CG32096

Feature Type

protein_coding_gene

FlyBase ID

FBgn0041096

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

rolling pebbles (rols) encodes a protein required for myoblast fusion in founder cells. it interacts with the membrane spanning Immunoglobulin superfamily protein encoded by kirre and with the product of Mhcl. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Interactive Fly

Also Known As

Rols7, Ants, l(3)08232, antisocial

Key Links

Genomic Location

Cytogenetic map

68F1-68F1

Sequence location

3L:12,008,225..12,064,859 [-]

Recombination map

3-38

RefSeq locus

NT_037436 REGION:12008225..12064859

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

enables cell adhesion molecule binding

inferred from physical interaction with FLYBASE:kirre; FB:FBgn0028369

(Kreiskother et al., 2006)

enables protein binding

inferred from physical interaction with FLYBASE:mbc; FB:FBgn0015513

(Chen and Olson, 2001)

inferred from physical interaction with FLYBASE:kirre; FB:FBgn0028369

(Chen and Olson, 2001)

inferred from physical interaction with FLYBASE:Actn; FB:FBgn0000667

(Kreiskother et al., 2006)

inferred from physical interaction with FLYBASE:blow; FB:FBgn0004133

(Kreiskother et al., 2006)

inferred from physical interaction with FLYBASE:zormin; FB:FBgn0052311

(Kreiskother et al., 2006)

enables structural constituent of muscle

inferred from mutant phenotype

(Kreiskother et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in anterior Malpighian tubule development

inferred from mutant phenotype

(Pütz et al., 2005)

involved_in border follicle cell migration

inferred from mutant phenotype

(Borghese et al., 2006)

involved_in myoblast fusion

inferred from mutant phenotype

(Bulchand et al., 2010, Menon et al., 2005, Chen and Olson, 2001, Menon and Chia, 2001, Rau et al., 2001)

involved_in sarcomere organization

inferred from direct assay

(Kreiskother et al., 2006)

inferred from mutant phenotype

(Kreiskother et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Menon et al., 2005)

colocalizes_with cell-cell junction

inferred from direct assay

(Menon et al., 2005)

located_in cytoplasm

inferred from direct assay

(Menon et al., 2005, Chen and Olson, 2001)

located_in Z disc

inferred from direct assay

(Kreiskother et al., 2006)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Summary (Interactive Fly)

RING-finger motif, ankyrin repeat and TR repeat protein - a signaling protein that interacts with the cytoplasmic domain of Dumbfounded and with Myoblast city - functions to integrate initial myoblast fusion with myotube structural organization

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\rols for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VTS9)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.42

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

rols-RA

FBtr0076056

NM_168488

5505

1569

rols-RB

FBtr0076053

NM_168487

6275

1900

rols-RC

FBtr0076057

NM_168489

5832

1670

rols-RD

FBtr0076054

NM_140285

6026

1569

rols-RE

FBtr0076055

NM_168490

5849

1670

rols-RF

FBtr0310009

NM_001274807

5846

1669

rols-RG

FBtr0310010

NM_001274808

5972

1799

rols-RH

FBtr0334109

NM_001274809

5502

1568

rols-RI

FBtr0334110

NM_001274810

5969

1798

Additional Transcript Data and Comments

Reported size (kB)

7, 6 (northern blot)

(Menon and Chia, 2001)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

rols-PA

FBpp0075788

169.1

1569

8.23

FBpp0075785

204.6

1900

6.64

FBpp0075789

180.1

1670

8.46

FBpp0075786

169.1

1569

8.23

FBpp0075787

180.1

1670

8.46

FBpp0301715

179.9

1669

8.46

FBpp0301716

193.6

1799

6.40

FBpp0306229

168.9

1568

8.23

FBpp0306230

193.5

1798

6.40

M9PC91

NP_001261739

AGB94432

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1569 aa isoforms: rols-PA, rols-PD

1670 aa isoforms: rols-PC, rols-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

1900 (aa); 205 (kD)

(Menon and Chia, 2001)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\rols using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.00

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 9 -- 10

dorsal ectoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11

presumptive embryonic/larval muscle system & myoblast | subset

(Menon and Chia, 2001)

Malpighian tubule primordium

Comment: transcripts originating from downstream promoter

(Pütz et al., 2005)

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

anterior midgut primordium

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

dorsal pharyngeal muscle primordium

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

embryonic stage 11 -- 13

embryonic/larval pharynx

(Pütz et al., 2005)

anterior midgut primordium

(Pütz et al., 2005)

posterior midgut primordium

(Pütz et al., 2005)

somatic trunk mesoderm

(Pütz et al., 2005)

embryonic stage 11 -- 14

presumptive embryonic/larval muscle system | restricted

(Chen and Olson, 2001)

embryonic stage 12 -- 15

myotube

(Menon and Chia, 2001)

embryonic Malpighian tubule

Comment: transcripts originating from downstream promoter

(Pütz et al., 2005)

embryonic stage 13

endoderm

(Pütz et al., 2005)

visceral trunk mesoderm

(Pütz et al., 2005)

embryonic stage 13 -- 17

endoderm

(Menon and Chia, 2001)

embryonic stage 15

muscle attachment site

(Chen and Olson, 2001)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis

border follicle cell

(Borghese et al., 2006)

Additional Descriptive Data

rols is enriched in border follicle cells relative to follicle cells.

(Borghese et al., 2006)

rols transcription from the upstream promoter (called rols7) is observed in the differentiating embryonic somatic and visceral mesoderm. Transcription from the downstream rols promoter (called rols6) is strong in early embryonic stage 11 between the ectodermal hindgut anlage and the posterior midgut primordium, where the renal tubes originate. Transcription continues throughout Malpighian tubule development.

(Pütz et al., 2005)

Transcript is first detected in the progenitors of visceral, somatic and pharyngeal muscles. Visceral mesoderm expression decreases during germband shortening while somatic mesoderm expression persists. At stage 15 rols transcript is no longer detected in the mesoderm but rather in muscle attachment sites along the segmental border.

(Chen and Olson, 2001)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

presumptive embryonic/larval muscle system & myoblast | subset

• cytoplasm

(Menon and Chia, 2001)

embryonic stage 11 -- 14

presumptive embryonic/larval muscle system | restricted

• cytoplasm | restricted

(Chen and Olson, 2001)

embryonic stage 12 -- 15

myotube

• cytoplasm & plasma membrane

(Menon and Chia, 2001)

embryonic stage 14

muscle founder cell

(Jin et al., 2011)

embryonic stage 15

muscle attachment site

• cytoplasm | restricted

(Chen and Olson, 2001)

Additional Descriptive Data

The protein is detected in discrete aggregates in the cytoplasm of founder muscle progenitor cells.

(Chen and Olson, 2001, Menon and Chia, 2001)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in apical plasma membrane

inferred from direct assay

(Menon et al., 2005)

colocalizes_with cell-cell junction

inferred from direct assay

(Menon et al., 2005)

located_in cytoplasm

inferred from direct assay

(Menon et al., 2005, Chen and Olson, 2001)

located_in Z disc

inferred from direct assay

(Kreiskother et al., 2006)

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.1}rols^{MI02479-TG4.1}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult glial cell | subset

(Lee et al., 2018)

Reporter: P{rols-Gal4.7}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15 -- 17

larval somatic muscle cell

(Stute et al., 2006)

larval visceral muscle cell

(Stute et al., 2006)

embryonic stage 12 -- 14

muscle founder cell

(Stute et al., 2006)

somatic trunk mesoderm

(Stute et al., 2006)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\rols in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\rols

View exonic expression by tissue for Dmel\rols

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0041096

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39368/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041096

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041096

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041096

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32096

Flygut - An atlas of the Drosophila adult midgut

FBgn0041096

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\rols

Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 36 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of rols

Transgenic constructs containing regulatory region of rols

Aberrations (Deficiencies and Duplications) ( 11 )

Inferred from experimentation ( 11 )

Gene not disrupted in

Df(3L)vin2

(Chen and Olson, 2001)

Df(3L)vin3

(Chen and Olson, 2001)

Gene duplicated in

Dp(3;2)GV-CH321-52G11

(Guo et al., 2019)

Inferred from location ( 10 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal (with Df(3L)BK9)

rols^08232ex18

lethal (with Df(3L)ED4475)

rols^{MI02479-TG4.1}

lethal | recessive

lethal - all die during embryonic stage

rols^{MI02479-TG4.1}

lethal - all die during embryonic stage (with Df(3L)ED4475)

rols^{MI02479-TG4.1}

lethal - all die during embryonic stage | recessive

partially lethal - majority live

rols^5a

viable

rols^LA00796

Sterility

Allele

fertile

rols^5a

Other Phenotypes

Allele

abnormal courtship behavior, with Scer\GAL4^repo

some die during embryonic stage | recessive

Phenotype manifest in

Allele

border follicle cell | somatic clone

rols^T627

dorsal acute muscle & myotube

embryonic/larval midgut longitudinal visceral muscle cell | embryonic stage

rols^AD328

embryonic/larval midgut longitudinal visceral muscle cell | embryonic stage (with Df(3L)BK9)

rols^XX117

embryonic/larval pharynx

rols⁰⁸²³²

embryonic anterior Malpighian tubule

embryonic Malpighian tubule

embryonic midgut chamber | embryonic stage 16

rols^XX117

embryonic midgut constriction 1

rols^T627

embryonic myoblast

embryonic myoblast (with Df(3L)BK9)

rols^XX117

embryonic myoblast (with Df(3L)vin4)

embryonic somatic muscle cell

longitudinal trunk visceral muscle primordium | embryonic stage 12 (with Df(3L)BK9)

rols^XX117

Malpighian tubule main body primordium

muscle cell of A1-7 dorsal acute muscle 1 | embryonic stage 15 (with Df(3L)BK9)

rols^08232ex18

NMJ bouton | increased number | third instar larval stage, with Scer\GAL4^C57

presumptive embryonic/larval digestive system

rols^unspecified

presumptive embryonic/larval muscle system (with Df(3L)BK9)

rols^08232ex18

visceral muscle fiber | embryonic stage

rols^T627

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (37)

Hsap\TANC1

11 of 14

Yes

Hsap\TANC2

11 of 14

Yes

Hsap\ANKRD50

2 of 14

Hsap\ANKRD65

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ANKRD31

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\RNF225

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (23)

Rnor\Tanc1

10 of 14

Yes

Rnor\Tanc2

10 of 14

Yes

Rnor\Ankrd50

2 of 14

Rnor\Ankrd65

2 of 14

Yes

Rnor\Aipl1

1 of 14

Rnor\Aip

1 of 14

Rnor\Ankrd53

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (23)

Mmus\Tanc1

10 of 14

Yes

Mmus\Tanc2

10 of 14

Yes

Mmus\Ankrd65

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (16)

Xtro\tanc1

8 of 13

Yes

Xtro\tanc2

6 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ttc9b

1 of 13

Xtro\ttc12

1 of 13

Xtro\unc45b

1 of 13

Danio rerio (Zebrafish) (25)

Drer\tanc1b

10 of 14

Yes

Drer\tanc2a

10 of 14

Yes

Drer\tanc1a

9 of 14

Yes

Drer\tanc2b

9 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\anks4b

1 of 14

Drer\asb14b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (27)

Cele\T28D6.4

2 of 14

Yes

Cele\Y38C1AA.19

2 of 14

Yes

Cele\aipr-1

1 of 14

Cele\arc-1

1 of 14

Cele\B0416.4

1 of 14

Yes

Cele\B0416.10

1 of 14

Yes

Cele\C30F2.2

1 of 14

Yes

Cele\C32E8.1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\F53F8.3

1 of 14

Yes

Cele\fkb-6

1 of 14

Cele\rnf-1

1 of 14

Yes

Cele\T01C3.3

1 of 14

Yes

Cele\T01G5.7

1 of 14

Yes

Cele\T20F5.6

1 of 14

Yes

Cele\T22B7.8

1 of 14

Yes

Cele\unc-45

1 of 14

Cele\Y6D1A.2

1 of 14

Yes

Cele\Y45F10B.8

1 of 14

Cele\Y45F10B.9

1 of 14

Cele\Y51F10.2

1 of 14

Yes

Cele\ZK993.2

1 of 14

Yes

Cele\ZK1240.2

1 of 14

Yes

Cele\ZK1240.9

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (17)

Agam\AgaP_AGAP004244

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (26)

Atha\ANK1

1 of 13

Yes

Atha\AT1G05640

1 of 13

Yes

Atha\AT1G07710

1 of 13

Yes

Atha\AT1G10340

1 of 13

Yes

Atha\AT1G34050

1 of 13

Yes

Atha\AT1G56090

1 of 13

Yes

Atha\AT1G69330

1 of 13

Yes

Atha\AT2G24600

1 of 13

Yes

Atha\AT2G31820

1 of 13

Yes

Atha\AT3G09550

1 of 13

Yes

Atha\AT4G03500

1 of 13

Yes

Atha\AT5G48570

1 of 13

Yes

Atha\AT5G54710

1 of 13

Yes

Atha\AT5G60070

1 of 13

Yes

Atha\ITN1

1 of 13

Yes

Atha\KEG

1 of 13

Yes

Atha\PAS1

1 of 13

Yes

Atha\PP5.2

1 of 13

Yes

Atha\ROF1

1 of 13

Yes

Atha\SQN

1 of 13

Yes

Atha\TOC64-III

1 of 13

Yes

Atha\TPR5

1 of 13

Yes

Atha\TPR6

1 of 13

Yes

Atha\TPR7

1 of 13

Yes

Atha\TTL1

1 of 13

Yes

Atha\TWD1

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\CNS1

1 of 13

Yes

Scer\CPR6

1 of 13

Yes

Scer\STI1

1 of 13

Yes

Scer\TOM71

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:rols. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (12)

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

roq

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

intellectual developmental disorder with autistic features and language delay, with or without seizures

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

rols^HMC04426

model of intellectual developmental disorder with autistic features and language delay, with or without seizures

CEC

(Guo et al., 2019)

rols^HMJ22326

model of intellectual developmental disorder with autistic features and language delay, with or without seizures

CEC

(Guo et al., 2019)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

TANC2; tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2

model of intellectual developmental disorder with autistic features and language delay, with or without seizures

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

rols⁰⁸²³²

ameliorates alveolar rhabdomyosarcoma

modeled by Hsap\FOXO1::Hsap\PAX7^UAS.cGa

(Avirneni-Vadlamudi et al., 2012)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TANC1; tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 1

11 of 14

611397

TANC2; tetratricopeptide repeat, ankyrin repeat and coiled-coil containing 2

11 of 14

TETRATRICOPEPTIDE REPEAT-, ANKYRIN REPEAT-, AND COILED-COIL-CONTAINING PROTEIN 2; TANC2

INTELLECTUAL DEVELOPMENTAL DISORDER WITH AUTISTIC FEATURES AND LANGUAGE DELAY, WITH OR WITHOUT SEIZURES; IDDALDS

intellectual developmental disorder with autistic features and language delay, with or without seizures

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

rols - Actn

two hybrid

(Kreiskother et al., 2006)

rols - Mhcl

two hybrid

(Bonn et al., 2013)

rols - blow

two hybrid

(Kreiskother et al., 2006)

rols - kirre

anti tag coimmunoprecipitation, anti tag western blot, two hybrid

(Bulchand et al., 2010, Kreiskother et al., 2006, Chen and Olson, 2001)

rols - mbc

anti tag coimmunoprecipitation, anti tag western blot

(Chen and Olson, 2001)

rols - zormin

two hybrid

(Kreiskother et al., 2006)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

peb

enhanceable

rols

(Wilk et al., 2004)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64728

DroID - A comprehensive database of gene and protein interactions.

FBgn0041096

MIST (protein-protein) - An integrated Molecular Interaction Database

39368

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8WS24

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-38

Cytogenetic map

68F1-68F1

Sequence location

3L:12,008,225..12,064,859 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

68F1-68F1

Limits computationally determined from genome sequence between P{PZ}CycA⁰²⁴⁶¹&P{PZ}CycA⁰³⁹⁴⁶ and P{PZ}rols⁰⁸²³²&P{lacW}l(3)j2D3^j2D3

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

68E-68F

(Chen, 2001.10.29)

68F1-68F2

(Menon, 2001.8.18)

68F1-68F3

(determined by in situ hybridisation)

(Spradling et al., 1999)

68F1-68F3

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-38

(FlyBase, 2016)

3-30.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

92197

y¹ w^* P{UAS-rols.7}1

58262

y¹ v¹; P{TRiP.HMJ22326}attP40

35988

y¹ w^*; Mi{MIC}rols^MI02479

37687

y¹ w^*; Mi{MIC}rols^MI04487

22194

y¹ w^*; P{Mae-UAS.6.11}rols^LA00796

11027

P{lArB}rols^A490.2M3 ry⁵⁰⁶/TM3, Sb¹

64471

y¹ w^*; Mi{PT-GFSTF.1}rols^{MI02479-GFSTF.1}

56986

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04426}attP40

76150

y¹ w^*; Mi{Trojan-GAL4.1}rols^{MI02479-TG4.1}/TM3, Sb¹ Ser¹

Kyoto

120796

y^* w^*; P{Mae-UAS.6.11}rols^LA00796

NIG-Fly

HMJ22326

y¹ v¹; P{TRiP.HMJ22326}attP40/CyO

VDRC

v330613

P{VSH330613}attP40

More stocks available...

Genomic Clones (45)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (27)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH15583

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0041096

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP02947

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0041096

Antibody Information

Laboratory Generated Antibodies

monoclonal

(Kesper et al., 2007)

polyclonal

(Kreiskother et al., 2006, Chen and Olson, 2001, Menon and Chia, 2001)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The ankyrin repeats of the rols protein are essential for kirre-dependent translocation, whereas the TPR/coiled-coil region functions after translocation.

(Menon et al., 2005)

rols acts during myoblast fusion to link the initial event of myoblast attraction with the downstream event of myotubule structural organisation.

(Menon and Chia, 2001)

rols mutants are defective in myoblast fusion.

(Menon and Chia, 2001)

Mutations in rols result in severe defects in myoblast fusion.

(Rau et al., 2001)

rols is involved in muscle fusion in the developing embryo.

(Rau et al., 2000)

The fusion process during muscle development is severely disrupted in mutant embryos.

(Rau et al., 2000)

Complementation groups l(3)68Fa, l(3)68Fb, l(3)68Fc, l(3)68Fd, l(3)68Fe, Nrx-IV, l(3)69Aa and l(3)69Ab isolated by Hoogwerf et al. (FBrf0048238) probably correspond to complementation groups l(3)68Fg, l(3)j2D3, l(3)68Fh, rols, l(3)68Fi, Nrx-IV, l(3)69Ah and l(3)69Ai isolated by Baumgartner at al, 1996, Cell 87: 1059--1068, but a one to one correspondence cannot be made as Hoogwerf mutations are apparently lost.

(Bellen, 1997.1.14)

Complementation group isolated during complementation analysis of Nrx-IV region.

(Baumgartner et al., 1996)

Relationship to Other Genes

Source for database merge of

Source for merge of: rols l(3)08232

(Rau et al., 2001)

Source for merge of: rols CG12277

(FlyBase, 1996-)

Source for merge of: rols anon-WO0257455.19

(FlyBase, 1996-)

Source for merge of: rols CG17155 CG5679

(FlyBase, 1996-)

Additional comments

Annotations CG12277, CG17155, CG5679 merged as CG32096 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

"CG17155" is a candidate gene for "E(sda)H".

(Zhang et al., 2002)

Source for merge of rols CG12277 was sequence identity ( date:011221 ).

(FlyBase, 1996-)

Source for merge of rols anon-WO0257455.19 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Source for merge of rols CG17155 CG5679 was sequence comparison ( date:011124 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

The gene is named "rolling pebbles" after the mutant phenotype; mutant embryos have a scattered distribution of unfused myoblasts.

(Rau et al., 2001)

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

ANTS

(Menon et al., 2005, Chen et al., 2003, Donaldson, 2003, Chen and Olson, 2002, Chen and Olson, 2001)

Ants

(Lee and Chen, 2019, Abmayr and Pavlath, 2012, Dobi et al., 2011, Jin et al., 2011, Chen et al., 2007, Kim et al., 2007, Chen and Olson, 2005, Chen and Olson, 2004)

CG12277

(Molnar et al., 2006)

CG17155

(Zhang et al., 2002)

CG32096

(Meadows, 2018.10.31, Abmayr and Pavlath, 2012, Ni et al., 2010.12.1)

CG5679

Rol/Ants

(Taylor, 2003)

Rols

(Dubey et al., 2020, Bothe and Baylies, 2016, Dobi et al., 2011, Bulchand et al., 2010, Kreiskother et al., 2006, Menon et al., 2005)

Rols6

(Kreiskother et al., 2006)

Rols7

(Bonn et al., 2013, Bonn et al., 2013, Abmayr and Pavlath, 2012, Jin et al., 2011, Chen et al., 2007, Griemert et al., 2007, Kesper et al., 2007, Kreiskother et al., 2006, Chen and Olson, 2005, Menon et al., 2005, Chen and Olson, 2004)

anon-WO0257455.19

ants

(Ruan et al., 2024, Borghese et al., 2006, Hossain et al., 2005, Dworak and Sink, 2002, Taylor, 2002, Chen, 2001.10.29, Chen and Olson, 2001)

l(3)08232

(Birchler et al., 2001, Spradling et al., 1999, Bellen, 1997.1.14, BDGP Project Members, 1994-1999)

l(3)68Fd

(Baumgartner et al., 1996)

l(3)j2A6

rols

(Bischoff et al., 2025, Chen et al., 2025, de Queiroz et al., 2025, Luo et al., 2025, Trujillo et al., 2024, Burghardt et al., 2023, Fanara et al., 2023, Marcogliese et al., 2022, Rout et al., 2022, Kvon et al., 2021, Salim et al., 2021, Walls et al., 2020, Guo et al., 2019, Lee et al., 2018, Cannavò et al., 2016, Gene Disruption Project members, 2016-, Shimaji et al., 2016, Duff et al., 2015, Gene Disruption Project members, 2015-, Hsu et al., 2015, Ahmad et al., 2014, Ashwal-Fluss et al., 2014, Bothe et al., 2014, Montgomery et al., 2014, Rudolf et al., 2014, Bonn et al., 2013, Avirneni-Vadlamudi et al., 2012, Du et al., 2011, Haralalka et al., 2011, Bulchand et al., 2010, Cook et al., 2009.11.25, Hazelett et al., 2009, Carrasco-Rando and Ruiz-Gómez, 2008, Christensen et al., 2008.7.11, Beckett and Baylies, 2007, Christensen et al., 2007.10.29, Estrada et al., 2007, Kesper et al., 2007, Massarwa et al., 2007, Richardson et al., 2007, Zeitouni et al., 2007, Borghese et al., 2006, Molnar et al., 2006, Stute et al., 2006, Hossain et al., 2005, Menon et al., 2005, Pütz et al., 2005)

rols7

(Lee and Chen, 2019)

rols\ants

(Artero et al., 2003)

Name Synonyms

Antisocial

(Dobi et al., 2011, Jin et al., 2011, Chen et al., 2007, Donaldson, 2003, Chen and Olson, 2002)

Rolling pebbles

(Dobi et al., 2011, Bulchand et al., 2010)

antisocial

(Ruan et al., 2024, Borghese et al., 2006, Menon et al., 2005, Taylor, 2002, Chen, 2001.10.29)

rolling pebbles

(Cannavò et al., 2016, Montgomery et al., 2014, Avirneni-Vadlamudi et al., 2012, Kreiskother et al., 2006, Burnette et al., 2005, Pütz et al., 2005, Rau et al., 2000)

rolling pebbles 7

(Menon et al., 2005)

Secondary FlyBase IDs

FBgn0010928
FBgn0011323
FBgn0036241
FBgn0036243
FBgn0036244
FBgn0052096
FBgn0063612
FBgn0063798

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 96 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39368

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VTS9

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VTS9

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0041096

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39368/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0041096

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0041096

FlyMine - An integrated database for Drosophila genomics

FBgn0041096

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG32096

MARRVEL_MODEL - MARRVEL (model organism gene)

39368

Linkouts

BioGRID - A database of protein and genetic interactions.

64728

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0041096

DroID - A comprehensive database of gene and protein interactions.

FBgn0041096

DRSC - Results frm RNAi screens

FBgn0041096

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0041096

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0041096

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG32096

Flygut - An atlas of the Drosophila adult midgut

FBgn0041096

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002665

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

39368

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q8WS24

References (123)

The following external sites may use different assemblies or annotations than FlyBase.

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