FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Gene: Dmel\PolE1
Open Close
General Information
Symbol
Dmel\PolE1
Species
D. melanogaster
Name
DNA polymerase epsilon subunit 1
Annotation Symbol
CG6768
Feature Type
protein_coding_gene
FlyBase ID
FBgn0264326
Gene Model Status
Current
Stock Availability
9 publicly available
Enzyme Name (EC)
DNA-directed DNA polymerase (2.7.7.7)
exodeoxyribonuclease I (3.1.11.1)
Gene Summary
DNA polymerase epsilon subunit 1 (PolE1) encodes the large subunit of DNA pol epsilon, an essential DNA polymerase participating with DNA pol alpha and DNA pol delta in cellular DNA replication. The C-terminal and N-terminal regions of the products of PolE1 have differential requirements in mitotic and endo-replicating cells. [Date last reviewed: 2019-09-26] (FlyBase Gene Snapshot)
All Summaries
Also Known As

DNApol-epsilon, DNApol-ε, DNApol-ε255, DNA polymerase ε, l(3)pl10

Key Links
Genomic Location
Cytogenetic map
94E13-94E13
Sequence location
Recombination map
3-78
RefSeq locus
NT_033777 REGION:23348128..23355163
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (20 terms)
Molecular Function (8 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
enables 3'-5'-DNA exonuclease activity
inferred from direct assay
(Oshige et al., 2004, Aoyagi et al., 1997)
enables DNA-directed DNA polymerase activity
inferred from direct assay
(Oshige et al., 2004, Aoyagi et al., 1997)
enables protein binding
inferred from physical interaction with UniProtKB:Q9VIT0
(Ruike et al., 2006)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
enables DNA binding
inferred from electronic annotation with InterPro:IPR006134, InterPro:IPR029703
(InterPro Project Members, 2004-)
enables DNA-directed DNA polymerase activity
inferred from electronic annotation with InterPro:IPR006172, InterPro:IPR013697, InterPro:IPR029703
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(GO Reference Genome Project, 2011-)
enables nucleic acid binding
inferred from electronic annotation with InterPro:IPR006172, InterPro:IPR036397
(InterPro Project Members, 2004-)
enables nucleotide binding
inferred from electronic annotation with InterPro:IPR006134, InterPro:IPR006172
(InterPro Project Members, 2004-)
enables single-stranded DNA 3'-5' DNA exonuclease activity
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(GO Reference Genome Project, 2011-)
enables zinc ion binding
inferred from electronic annotation with InterPro:IPR013697
(InterPro Project Members, 2004-)
Biological Process (10 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
involved_in DNA repair
inferred from mutant phenotype
(Verma et al., 2012)
involved_in DNA replication proofreading
inferred from direct assay
(Oshige et al., 2004)
involved_in DNA synthesis involved in DNA replication
inferred from direct assay
(Oshige et al., 2004)
involved_in DNA-templated DNA replication
inferred from direct assay
(Aoyagi et al., 1997)
involved_in endomitotic cell cycle
inferred from mutant phenotype
(Suyari et al., 2012)
involved_in mitotic cell cycle
inferred from mutant phenotype
(Suyari et al., 2012)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
involved_in base-excision repair, gap-filling
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(GO Reference Genome Project, 2011-)
involved_in DNA repair
inferred from electronic annotation with InterPro:IPR029703
(InterPro Project Members, 2004-)
involved_in DNA replication proofreading
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(GO Reference Genome Project, 2011-)
involved_in DNA replication
inferred from electronic annotation with InterPro:IPR013697, InterPro:IPR029703
(InterPro Project Members, 2004-)
involved_in leading strand elongation
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(GO Reference Genome Project, 2011-)
involved_in nucleotide-excision repair, DNA gap filling
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(GO Reference Genome Project, 2011-)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of epsilon DNA polymerase complex
inferred from physical interaction with FLYBASE:PolE2; FB:FBgn0035644
(Oshige et al., 2004)
located_in nucleus
inferred from direct assay
(Yamaguchi et al., 1995)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of epsilon DNA polymerase complex
inferred from biological aspect of ancestor with PANTHER:PTN000072300
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR029703
(InterPro Project Members, 2004-)
located_in nucleus
inferred from electronic annotation with InterPro:IPR013697
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the DNA polymerase type-B family. (Q9VCN1)
Protein Signatures (InterPro)
Catalytic Activity (EC/Rhea)
DNA-directed DNA polymerase activity
DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate (2.7.7.7)
single-stranded DNA 3'-5' DNA exonuclease activity
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates (3.1.11.1)
Summaries
Gene Snapshot
DNA polymerase epsilon subunit 1 (PolE1) encodes the large subunit of DNA pol epsilon, an essential DNA polymerase participating with DNA pol alpha and DNA pol delta in cellular DNA replication. The C-terminal and N-terminal regions of the products of PolE1 have differential requirements in mitotic and endo-replicating cells. [Date last reviewed: 2019-09-26]
Gene Group (FlyBase)
DNA POLYMERASE EPSILON -
DNA polymerase epsilon functions as a leading strand polymerase during nuclear DNA synthesis. It is a heterotetramer in which DNApol-ε255 encodes the catalytic subunit with polymerase and 3'-5' exonuclease proofreading activities. (Adapted from PMID:30005324.)
DNA POLYMERASE CATALYTIC SUBUNITS -
Multiple DNA polymerases are required for DNA replication and/or repair in eukaryotic cells. The alpha-primase, delta, epsilon, zeta and gamma polymerases exist as multi-subunit complexes in which one of the subunits has catalytic activity, whereas other DNA polymerases act as single molecules. (Adapted from PMID:30005324.)
3'-5' DNA EXONUCLEASES -
3'-5'-exodeoxyribonucleases catalyze the sequential degradation of double-stranded DNA acting progressively in a 3'-to-5' direction, releasing nucleoside 5'-phosphates.
Protein Function (UniProtKB)
Catalytic component of the DNA polymerase epsilon complex (PubMed:15135399). Participates in chromosomal DNA replication (PubMed:15135399). Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork (By similarity). Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication (PubMed:15135399). Has a role in the G1-S transition and/or S-phase progression of the mitotic cycle and endocycle progression (PubMed:11054539, PubMed:15135399, PubMed:21898761). Involved in DNA synthesis during DNA repair (By similarity). Plays roles in larval tissue development (PubMed:21898761, PubMed:22245183).
(UniProt, Q9VCN1)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\PolE1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VCN1)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Differences between protein sequence and SWP:Q9GV40 are in the center of a long ORF and may correspond to strain differences.

Gene model reviewed during 5.46

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
PolE1-RA
FBtr0084408
NM_079738
6849
2236
Additional Transcript Data and Comments
Reported size (kB)

8.0 (northern blot)

(Oshige et al., 2000)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
PolE1-PA
FBpp0083800
256.7
2236
6.42
Q9VCN1
NP_524462
AAF56126
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

2220 (aa); 255 (kD predicted)

(Oshige et al., 2000)

250 (kD observed)

(Aoyagi et al., 1997)
Comments

DNApol-ε protein was purified and characterized.

(Aoyagi et al., 1997)
External Data
Subunit Structure (UniProtKB)

Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit PolE1/DNApol-epsilon255 and the accessory subunits PolE2/DNApol-epsilon58, Chrac-14/DNApolE3 and PolE4.

(UniProt, Q9VCN1)
Domain

The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for maintenance of the complex.

The C-terminal domain (1001-2236 aa) is required for mitotic DNA replication in the eye but not for DNA endoreplication in salivary glands.

The CysA-type zinc finger is required for PCNA-binding.

The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes.

(UniProt, Q9VCN1)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\PolE1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.40

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
embryonic/larval Malpighian tubule
(Verma et al., 2012)
larval brain
(Verma et al., 2012)
wing disc
(Verma et al., 2012)
leg disc
(Verma et al., 2012)
embryonic/larval salivary gland
(Verma et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- pupal stage
(Oshige et al., 2000)
adult stage | female
(Oshige et al., 2000)
adult stage | male
(Oshige et al., 2000)
unfertilized egg stage
unfertilized egg
(Oshige et al., 2000)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood | male -- day 7 of adulthood | male
spermatozoon
(Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of epsilon DNA polymerase complex
inferred from physical interaction with FLYBASE:PolE2; FB:FBgn0035644
(Oshige et al., 2004)
located_in nucleus
inferred from direct assay
(Yamaguchi et al., 1995)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\PolE1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of PolE1
Transgenic constructs containing regulatory region of PolE1
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene not disrupted in
Df(3R)e-Gp4
(Akanksha et al., 2008)
Df(3R)GC14
(Akanksha et al., 2008)
Inferred from location ( 2 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4insc-Mz1407
lethal | pupal stage | recessive
lethal | recessive | third instar larval stage
lethal - all die before end of pupal stage, with Scer\GAL4Act5C.PU
lethal - all die before end of pupal stage, with Scer\GAL4Act.PU
lethal - all die before end of pupal stage | recessive
partially lethal - majority die | pupal stage
partially lethal - majority die | third instar larval stage
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
abnormal developmental rate | larval stage | recessive
abnormal DNA repair | first instar larval stage
abnormal DNA repair | third instar larval stage
abnormal endomitotic cell cycle, with Scer\GAL4l(1)BP-1
abnormal locomotor behavior | recessive | third instar larval stage
abnormal mitotic cell cycle, with Scer\GAL4ey.PU
abnormal neuroanatomy | larval stage, with Scer\GAL4insc-Mz1407
abnormal size | recessive | third instar larval stage
decreased cell number | recessive | somatic clone | third instar larval stage
decreased cell number | recessive | third instar larval stage
decreased cell size | recessive | third instar larval stage
decreased occurrence of cell division | larval stage, with Scer\GAL4ey.PU
melanotic mass phenotype | recessive | third instar larval stage
some die during pupal stage, with Scer\GAL4Act5C.PU
some die during pupal stage, with Scer\GAL4Act.PU
some die during pupal stage | recessive
visible, with Scer\GAL4ap-md544
visible, with Scer\GAL4ey.PU
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
embryonic/larval gut | third instar larval stage
embryonic/larval salivary gland | third instar larval stage
embryonic/larval salivary gland | third instar larval stage, with Scer\GAL4Act5C.PU
eye, with Scer\GAL4ey.PU
eye disc, with Scer\GAL4ey.PU
ganglion | third instar larval stage
ganglion mother cell | larval stage, with Scer\GAL4insc-Mz1407
imaginal disc
imaginal disc | somatic clone | third instar larval stage
imaginal disc | third instar larval stage, with Scer\GAL4Act5C.PU
larval brain | third instar larval stage, with Scer\GAL4Act5C.PU
larval neuroblast | larval stage, with Scer\GAL4insc-Mz1407
mesonotum, with Scer\GAL4pnr-MD237
ommatidium, with Scer\GAL4ey.PU
ommatidium | third instar larval stage
pupa
salivary gland, with Scer\GAL4l(1)BP-1
wing, with Scer\GAL4ap-md544
wing disc | third instar larval stage
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (4)
Hsap\POLE
14 of 14
Yes
Yes
Hsap\POLA1
1 of 14
No
No
Hsap\POLD1
1 of 14
No
No
Hsap\REV3L
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
Rnor\Pole
14 of 14
Yes
Yes
Rnor\Pola1
1 of 14
No
No
Rnor\Pold1
1 of 14
No
No
Rnor\Rev3l
1 of 14
No
No
Mus musculus (laboratory mouse) (4)
Mmus\Pole
13 of 14
Yes
Yes
Mmus\Pola1
1 of 14
No
No
Mmus\Pold1
1 of 14
No
No
Mmus\Rev3l
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (1)
Xtro\pole
11 of 13
Yes
Yes
Danio rerio (Zebrafish) (4)
Drer\pole
14 of 14
Yes
Yes
Drer\pola1
1 of 14
No
No
Drer\pold1
1 of 14
No
No
Drer\rev3l
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\pole-1
14 of 14
Yes
Yes
Cele\F10C2.4
1 of 14
No
No
Cele\rev-3
1 of 14
No
No
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP004615
12 of 12
Yes
Yes
Agam\AgaP_AGAP001929
1 of 12
No
No
Agam\AgaP_AGAP011731
1 of 12
No
No
Agam\AgaP_AGAP013386
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (6)
Atha\TIL1
13 of 13
Yes
Yes
Atha\TIL2
13 of 13
Yes
Yes
Atha\AT1G49180
1 of 13
No
No
Atha\AT5G63960
1 of 13
No
No
Atha\ICU2
1 of 13
No
No
Atha\REV3
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (4)
Scer\POL2
13 of 13
Yes
Yes
Scer\POL1
1 of 13
No
No
Scer\POL3
1 of 13
No
No
Scer\REV3
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (4)
Spom\cdc20
12 of 12
Yes
Yes
Spom\cdc6
1 of 12
No
No
Spom\pol1
1 of 12
No
No
Spom\rev3
1 of 12
No
No
Escherichia coli (enterobacterium) (1)
Ecol\polB
1 of 11
Yes
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:PolE1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (3)
PolA1
1 of 13
PolD1
1 of 13
PolZ1
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    PolE1pl10R
    exacerbates  neurodegenerative disease
    modeled by Zzzz\CAG127Q.UAS.Tag:HA
    (Sengupta and Lakhotia, 2006)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    POLE; DNA polymerase epsilon, catalytic subunit
    14 of 14
    174762
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Interaction Browsers
      View in FlyBase Interactions Browser   View in MIST tool (external link)
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      PolE1 - PolE2
      affinity chromatography technology, western blot
      (Oshige et al., 2004)
      PolE1 - Su(var)205
      proximity ligation assay, fluorescence microscopy
      (Vo et al., 2017)
      Summary of Genetic Interactions
      Interaction Browsers
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Subunit Structure (UniProtKB)
      Component of the DNA polymerase epsilon complex consisting of four subunits: the catalytic subunit PolE1/DNApol-epsilon255 and the accessory subunits PolE2/DNApol-epsilon58, Chrac-14/DNApolE3 and PolE4.
      (UniProt, Q9VCN1 )
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      FlyBase
      External Links
      External Data
      Class of Gene
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map
      3-78
      Cytogenetic map
      94E13-94E13
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      94E13-94E13
      Limits computationally determined from genome sequence between P{PZ}cnc03921 and P{PZ}l(3)0690606906
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      94E13-94F1-94E13-94F1
      (Sengupta and Lakhotia, 2006)
      94F6-95A1
      (determined by in situ hybridisation)
      (Oshige et al., 2000)
      Experimentally Determined Recombination Data
      Location

      3-78

      (FlyBase, 2016)

      3-80.3

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (9)
      Genomic Clones (15)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (4)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Yamaguchi et al., 1995)
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            Expression pattern and subcellular distribution of the protein during development is studied using antibodies.

            (Yamaguchi et al., 1995)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: DNApol-ε CG6768

            (FlyBase, 1996-)
            Additional comments

            Source for merge of DNApol-ε CG6768 was sequence comparison ( date:001216 ).

            (FlyBase, 1996-)
            Nomenclature History
            Source for database identify of

            Source for identity of: DNApol-ε255 DNApol-ε

            (FlyBase, 2009-)

            Source for identity of: PolE1 DNApol-ε255

            (Marygold et al., 2020)
            Nomenclature comments

            Renamed from 'DNApol-ε' to 'DNApol-ε255' to better distinguish from the other DNApol-ε subunit (DNApol-ε58) and to be consistent with the nomenclature of other DNA polymerase genes.

            (FlyBase, 2009-)
            Etymology

            Source for merge of: DNApol-ε l(3)pl10

            (Verma et al., 2012)
            Synonyms and Secondary IDs (26)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 51 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            References (70)